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Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Published by Universitas Nusa Nipa
ISSN : 2716151X     EISSN : 2722869X     DOI : http://dx.doi.org/10.55241/spibio
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Other
Jurnal ini fokus pada bidang kajian biologi sains dan pendidikan biologi. Kami menerima artikel berupa hasil penelitian maupun kajian pustaka yang original dan belum pernah dipublikasi.
Arjuna Subject : Umum - Umum
Articles 392 Documents
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Potensi Ekstrak Etanol Daun Afrika (Vernonia amygdalina Del.) terhadap Perbaikan Kerusakan Ginjal Tikus Putih (Rattus norvegicus) yang Diberikan Sopi Petra Willem Rodnels Melatunan; Chomsa Dintasari Umi Baszary; Meillisa Carlen Mainassy
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.723

Abstract

Excessive consumption of sopi in the long term can cause various chronic diseases such as kidney failure. This study aims to determine the potential of African leaf ethanol extract in repairing sopi-induced kidney damage in white rats. The experimental design used was a completely randomized design (CRD) with five treatments. The sample was 18 mice. Groups of mice K (-), K (+), P1, P2 and P3 were induced with 1.28 ml of sopi/animal. K (+) mice were given Furosemide as a comparison. Groups P1, P2 and P3 were given ethanol extract of African leaves at doses of 1.28, 2.56 and 5.12 ml/head/day respectively. The results obtained were analyzed using analysis of variance (ANOVA) then followed by the Tukey HSD post hoc test. The results of the study showed that there was an effect of reducing creatinine levels and improving kidney damage in white mice due to administration of African leaf ethanol extract, where the effective dose was 2.56ml/head/day. So it can be concluded that the ethanol extract of African leaves at a dose of 2.56 ml/head/day has the potential to reduce creatinine levels and repair sopi-induced kidney damage in white rats, the results of which are also the same as those shown by the drug Furosemide. However, giving higher doses will be toxic to the body.
Pengaruh Blended Learning terhadap Hasil Belajar Mahasiswa Jurusan Biologi FMIPA Universitas Negeri Makassar Adnan Adnan; Andi Rahmat Saleh; Dian Dwi Putri Ulan Sari Patongai; Suhardi Aldi
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.724

Abstract

Student learning outcomes are still a challenge in higher education, especially in a learning system that is still teacher-centered and lack interactivity. Therefore, it is necessary to improve the quality of learning through more responsive and flexible approaches, such as blended learning. This study aims to analyze the effect of implementing blended learning on the learning outcomes of biology students in the Faculty of Mathematics and Natural Sciences (FMIPA) at the University of Makassar (UNM). This study employed a quasi-experimental design with random sampling. The study population consisted of all Biology Department students at FMIPA UNM enrolled in the Cell and Molecular Biology course. The sample comprised 75 students divided into three classes: two experimental classes and one control class. The assessment instrument used was a learning outcome test in the form of multiple-choice questions. Data analysis was conducted descriptively and inferentially using a one-way ANOVA test. The results showed that Experimental Class 1, which implemented blended learning, achieved a higher average learning outcome score compared to Experimental Class 2 and the control class. The ANOVA test indicated a significant difference between the groups (p < 0.05). Thus, blended learning has a significant effect on student learning outcomes.
Efektivitas Pemanfaatan Media Online terhadap Partisipasi Mahasiswa dalam Kegiatan Sosial yang Berorientasi Lingkungan Emanuel Hanoe; Erlin Fatima Halek; Antonius T. T. Ruron; Yeremias Binsasi
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.726

Abstract

The presence of technology as a manifestation of the development of human resource potential, especially social media that can be used to communicate, disseminate information. Students as agents of change, are the group most vulnerable to internet dependence and utilize social media as the main tool for communication, participation in social and environmental actions. The purpose of this study was to determine student participation in environmentally oriented social activities. The research method used a qualitative descriptive approach. Qualitative descriptive data analysis techniques with data reduction, interpretation and verification stages. The results of observations found problems in the Biology Education study program in the field of science and the environment experiencing a decline in terms of quality. Student involvement in social actions as agents of change has not been seen in overcoming environmental problems. The low participation of students in environmental activities in the community, such as conservation activities, tree planting, environmental cleanups or environmental campaigns due to lack of motivation, social interaction and understanding of science/environmental learning. Types of environmental activities known by students and practiced in organizational or group activities such as: urban waste management, the use of chemical pesticides, drought and environmentally friendly schools. Online media used by students to socialize environmental campaign activities such as using YouTube, Instagram and Facebook. 
Evaluasi Tepung dan Infusum Kacang Hijau (Vigna radiata) sebagai Media Alternatif Pertumbuhan Staphylococcus aureus Febri Sembiring; Suparni; Dewi Setiyawati; Wardati Humaira; Sri Widia Ningsih; Nin Suharti; Astrid Siska Pratiwi
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.727

Abstract

Limited access to and the high cost of commercial growth media have driven the development of alternative media based on locally available materials. Although several legume-based materials have been reported to support bacterial growth, the use of mung beans (Vigna radiata) as an alternative medium—particularly in the form of flour and infusion—and comparisons of their effectiveness against standard media remain limited. This study aims to evaluate the potential of these two forms as growth media for Staphylococcus aureus. This was an experimental study conducted from March to May 2024 at the Microbiology Laboratory of the Medan Public Health Polytechnic (Poltekkes Kemenkes). The method used was growth analysis via the Total Plate Count (TPC) method. The results showed that the average TPC of S. aureus on mung bean flour medium was 1.07 × 10⁷ CFU/mL, and on mung bean infusion medium it was 4.7 × 10⁶ CFU/mL, whereas TSA medium, as the standard medium, yielded 5.8Z× 10⁶ CFU/mL. This indicates that both forms of the mung bean alternative media were capable of supporting optimal bacterial growth, with the flour medium yielding a higher cell count compared to the standard medium (TSA). Morphologically, the colonies on the alternative media were smaller (±2 mm, yellowish-white) compared to those on TSA (±3 mm, yellow). Thus, it can be concluded that mung bean flour has the potential to be an effective alternative medium for growing S. aureus compared to the infusion medium, which is less optimal due to the possible degradation of nutrients during the heating process.
Isolasi dan Uji Aktivitas Antimikrobia Bakteri Endofit Akar Mangrove Rhizophora mucronata di Kawasan Ekowisata Mangrove Kupang Alexander Tage; Chatarina Gradict Semiun; Yulita Iryani Mamulak
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.730

Abstract

Semi-arid mangrove ecosystems harbor significant potential as sources of endophytic bacteria producing adaptive antimicrobial compounds. This study aimed to isolate and evaluate the antimicrobial activity of endophytic bacteria from Rhizophora mucronata prop and lateral roots across three management zones (core, buffer, and utilization) in the Kupang Mangrove Ecotourism Area. Sampling was conducted in March–April 2026, followed by root surface sterilization and isolation on Nutrient Agar. Phenotypic characterization was performed macroscopically, microscopically, and biochemically, while antimicrobial activity was assessed using the Kirby-Bauer disk diffusion method against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. From 69 initial colonies, 18 pure isolates were obtained, with Bacillus sp. as the dominant genus (44.4%). Notably, Bacillus sp. isolates ZS-04 and ZI-05 exhibited the most potent bioactivity, yielding very strong (>20 mm) inhibition zones against S. aureus and strong (10–20 mm) inhibition against E. coli. One-way ANOVA revealed no significant zonal differences for S. aureus (p = 0.785), whereas a statistically significant zonal effect was observed for E. coli (p = 0.045), particularly between the utilization and buffer zones. These findings confirm that endophytic Bacillus sp. from R. mucronata roots, particularly isolates ZS-04 and ZI-05, produce bioactive compounds with strong, targeted antibacterial activity against Gram-positive pathogens, highlighting semi-arid mangrove endophytes as promising, stress-adapted reservoirs for novel natural antimicrobial agents.
Korelasi Kemampuan Berpikir Kritis Siswa Kelas X SMA Negeri 1 Labuhan Deli terhadap Literasi Lingkungan pada Materi Pencemaran Lingkungan Aqila Shabira Purba; Widya Arwita
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.731

Abstract

The low level of students' critical thinking skills has the potential to affect the level of environmental literacy. This study aims to determine the level of critical thinking skills, environmental literacy levels, positive relationships and how much critical thinking contributes to environmental literacy in grade X students of SMA Negeri 1 Labuhan Deli on environmental pollution material. The study used a quantitative approach with a correlational method on 62 students selected through a purposive sampling technique. The research instruments were essay tests and questionnaires, while the data were analyzed using the Pearson Product Moment correlation test and simple linear regression assisted by SPSS 26. The results of the prerequisite test showed that the data were normally distributed, linear, and homogeneous. Students' critical thinking skills were in the high category with a score of 72, while environmental literacy was in the high category with a score of 81. The results of the correlation test showed a very strong and positive relationship between critical thinking skills and environmental literacy (r = 0.879; p < 0.05) and a coefficient of determination of 77.3%. It was concluded that the higher students' critical thinking skills, the higher their environmental literacy. This research has implications for the importance of strengthening critical thinking skills in learning to improve students' environmental literacy.
Inventarisasi Jenis Talas-Talasan (Araceae) di Tepian Sungai Lembah Gunung Mandiangin KHDTK Universitas Lambung Mangkurat Muhammad Maulana; Bunda Halang; Mahrudin Mahrudin
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.746

Abstract

Information on plant species in special forest areas is important to support the conservation and management of biological resources, so that plant inventory needs to be carried out to determine their existence and characteristics. This study was conducted with the aim of describing the taro (Araceae) species found on the banks of the Mandiangin Mountain Valley River, KHDTK, Lambung Mangkurat University. Sampling of taro (Araceae) plants was carried out through the exploration method, namely by exploring the path around the banks of the Mandiangin Mountain Valley River. The results of the study found 18 types of taro (Araceae) which are classified into several genera, including Caladium bicolor, Caladium bicolor (Aiton) Vent, Caladium bicolor var. florida, Aglaonema commutatum, Aglaonema sp., Syngonium podophyllum, Syngonium angustatum, Colocasia esculenta (L.), Colocasia sp., Philodendron erubescens, Philodendron bipinnatifidum, Xanthosoma sagittifolium, Amydrium medium, Alocasia macrorrhiza, Dieffenbachia sp., Amorphophallus sp., Homalomena sp., and Monstera deliciosa. The diversity of these species indicates that the humid, shady, and organic-rich riverbank environment is a suitable habitat for the growth of taro (Araceae) species. Environmental factors such as soil pH, soil moisture, temperature, altitude, and light intensity also support the existence and distribution of taro (Araceae) plants in this area. The results of this study are expected to be basic data on biodiversity, a reference for identifying local taro (Araceae), and supporting materials for learning and management of the KHDTK area of ​​Lambung Mangkurat University.
Pengaruh Pembelajaran Inkuiri Terbimbing Berbantuan Worksheet terhadap Kemampuan Berpikir Kritis Siswa pada Materi Bioteknologi Koriatul Jannah Siregar; Wina Dyah Puspita Sari
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.749

Abstract

Critical thinking skills are among the essential competencies required for students to meet the challenges of the 21st century. However, students' critical thinking skills in biotechnology remain relatively low and need to be enhanced through the implementation of learning models that promote active participation in the learning process. This study aims to determine the effect of the Guided Inquiry learning model assisted by Student Worksheets on students' critical thinking skills in Biotechnology material at UPT SMP Negeri 35 Medan. This study used a quasi-experimental method with a non-equivalent control group design. The population consisted of all grade IX students of UPT SMP Negeri 35 Medan. The sample was selected using purposive sampling, with class IX-1 as the experimental class and class IX-3 as the control class. The research instrument was a validated two-tier multiple-choice test designed to measure students' critical thinking skills. Data analysis using the Independent Samples T-test showed a Sig. (2-tailed) value of 0.000 < 0.05, indicating a significant effect of the Guided Inquiry model assisted by worksheets on students' critical thinking skills. The experimental class showed a higher average improvement (55.38%) than the control class (44.51%). All indicators of critical thinking clarification, assessment, inference, and strategy improved significantly in the experimental class. Therefore, the Guided Inquiry learning model assisted by worksheet is effective in improving students' critical thinking skills in Biotechnology material.
Pengaruh Problem-Based Learning Berbantuan Video terhadap Keterampilan Berpikir Kritis dan Literasi Lingkungan Siswa dalam Pembelajaran Ekosistem Anisa Pramesti; Widya Arwita
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.735

Abstract

The low level of students' critical thinking skills and environmental literacy, as reflected in Indonesia's poor performance in PISA, along with the still-dominant use of conventional, teacher-centered learning methods, served as the background of this research. Which limit students' active participation and higher-order thinking development in ecosystem learning. This study aims to examine the effect of the Problem-Based Learning (PBL) model assisted by video media on students' critical thinking skills and environmental literacy in ecosystem learning. This study employed a quasi-experimental research design with a non-equivalent control group design. The research was conducted at SMAS Angkasa 1 Lanud Soewondo Medan, involving Grade X students in the even semester of the 2025/2026 academic year. Data were collected through pretest and post-test instruments measuring critical thinking skills and environmental literacy, which encompassed three components: knowledge, attitude, and behavior. The data analysis was performed using descriptive statistics and an independent sample t-test. The results indicate that students in the experimental class who were taught using the video-assisted PBL model demonstrated significantly higher critical thinking skills and environmental literacy scores compared to students in the control class who received conventional instruction. These findings contribute to the development of science, particularly by providing empirical evidence on the effectiveness of video-assisted PBL in enhancing critical thinking skills and environmental literacy, and by offering a reference for future research and innovation in biology education.
Hubungan antara Tingkat Pengetahuan tentang Keanekaragaman Hayati dengan Sikap Siswa terhadap Konservasi Priska Amanda; Tri Harsono
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.737

Abstract

Biodiversity is one of the important components in maintaining the balance of ecosystems and environmental sustainability. However, the low awareness of students towards environmental conservation is still a problem that needs to be considered in biology learning. This study aimed to determine the relationship between the level of knowledge about biodiversity and students’ attitudes toward conservation in Grade X of SMA Negeri 2 Medan, Academic Year 2025/2026. The method used in this study was quantitative research with a correlational approach. The population consisted of 426 students, while the sample consisted of 70 students. The sampling technique used was purposive sampling. The instruments used were multiple-choice tests to measure the level of biodiversity knowledge, as well as multiple-choice tests and observation sheets to measure students’ attitudes toward conservation. Data analysis was conducted using the Pearson Product Moment correlation test. The results of this study indicated that there was a significant relationship between the level of knowledge about biodiversity and students’ attitudes toward conservation. The correlation coefficient value of 0.733 indicated that the relationship between the two variables was categorized as strong and had a positive direction.

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