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Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Published by Universitas Nusa Nipa
ISSN : 2716151X     EISSN : 2722869X     DOI : http://dx.doi.org/10.55241/spibio
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Other
Jurnal ini fokus pada bidang kajian biologi sains dan pendidikan biologi. Kami menerima artikel berupa hasil penelitian maupun kajian pustaka yang original dan belum pernah dipublikasi.
Arjuna Subject : Umum - Umum
Articles 193 Documents
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Pemanfaatan dan Pengelolaan Keanekaragaman Tumbuhan di Persawahan berbasis Kearifan Lokal Masyarakat Adat Using Kemiren, Banyuwangi Nandini , Alda Alfiah Dwi; Utomo, Agus Prasetyo; Priantari, Ika
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.607

Abstract

Plant diversity in agricultural landscapes plays a vital role in maintaining ecosystem sustainability and local food security. This study aims to analyze the utilization, management, and conservation of plant diversity by the Using Indigenous community in Kemiren Village, Banyuwangi. A qualitative approach was used, with data collected through interviews, observation, and documentation. A total of 10 informants (2 key informants and 8 recommended informants) were selected using purposive sampling and snowball sampling techniques. The informant selection criteria were traditional elders, local farmers, and the Using community of Kemiren Village who possessed local knowledge regarding the types and uses of plant diversity found in their rice fields. Data validity was tested using cross-reference information, repeated information, and data triangulation. Data analysis was conducted qualitatively. The research results show that the community uses 23 plants for food, medicine, traditional ceremonies, animal feed and building materials. Plant management is carried out through land clearing, seed preparation, planting, maintenance, and harvesting. The steps combining traditional and modern technique, and supported of local rituals such as labuhan and adeg-adeg.  Conservation practices include the use of organic fertilizers, post-harvest waste management, crop diversification, natural pest control, and water management based on local wisdom. The integration of local knowledge and conservation practices has been shown to play a role in conserving biodiversity, supporting food security, and promoting sustainable agriculture.
Penerapan Model Problem Based Learning (PBL) Berbantuan Media Padlet terhadap Asesmen Sumatif siswa Biologi Fase F Safitri, Ifla; Meizeli, Annika; Zikra, Zikra
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.625

Abstract

This research is motivated by the learning process using group discussion methods, lectures and scientific approaches. The ongoing discussion activities have not run well because they are not heterogeneous so that student involvement in group discussions is not optimal. This results in low student learning outcomes. By implementing a direct learning model that is not optimal, it makes student motivation and understanding of the material low. This study aims to determine the application of the Problem Based Learning (PBL) model assisted by Padlet media on Biology learning outcomes in phase F students at MAN1 West Pasaman (Even Semester 2024/2025). The research method is experimental research. Using two sample classes, namely the experimental class and the control class. The learning process in the experimental class uses the Problem Based Learning (PBL) model assisted by Padlet media while the control class uses a scientific approach. The design used is Randomized Control Group Posttest Only. The sample consists of two classes (experimental and control classes), selected by purposive sampling. The research instrument is in the form of multiple-choice questions through summative assessment. Data were analyzed using the t test. The summative assessment was higher (75.82 > 58.72), there was a significant (t??? > t table). It can be concluded that the Problem Based Learning (PBL) learning model assisted by Padlet media can improve learning outcomes in the summative assessment of students in phase F of MAN 1 West Pasaman. The implications of this research are: 1) Biology teachers can use the Problem Based Learning model assisted by Padlet media to improve students' biology learning outcomes, 2) Other researchers can apply the Problem Based Learning model assisted by Padlet media to different materials and different variables.
Agensia Hayati Antifungi Ekstrak Batang Faloak (Sterculia quadrifida R.Br.) terhadap Fusarium oxysporum Asmiati, Asmiati; Linome, Yampianus; Ernawati, Ernawati
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.672

Abstract

Fusarium oxysporum is one of the fungi that cause diseases in plants, which can result in losses for farmers. Efforts to control fungi using synthetic fungicides can lead to pathogen resistance, environmental pollution, and even poisoning in humans. Therefore, the search for new sources of natural fungicides is considered necessary because they are safer and pose minimal risk.This study aims to determine the activity of faloak stem extract (Sterculia quadrifida R.Br.) as a fungicidal biological agency in inhibiting the growth of Fusarium oxysporum. This research has been carried out at the Biology Laboratory, Muhammadiyah University of Kupang. This study is a laboratory experimental study with 4 treatments and 4 repeats, namely A as a control, B = 0.5 ml, C = 0.75 ml, and D = 1 ml. The parameter measured was the diameter of the colony of F.oxysporium during 5 days of incubation. Colony diameter data were analyzed with Anova and further tested with Duncan. The research results showed that the lowest average colony diameter was obtained from treatment D, measuring 1.01 cm, with very strong antifungal activity. Meanwhile, fungal colony growth in the control medium reached 8.5 cm, indicating no antifungal activity. Further analysis revealed that each treatment was significantly different. This indicates that faloak stem extract is capable of inhibiting the growth of F. oxysporum, and it can therefore be concluded that faloak stem extract has potential as a biological fungicidal agent against Fusarium oxysporum.
Keanekaragaman Spesies Famili Myrtaceae Tepian Sungai Barito, Desa Sungai Gampa Kabupaten Barito Kuala Kalimantan Selatan Sasmita, Sasmita; Dharmono, Dharmono; Suyidno, Suyidno
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.691

Abstract

The riparian zone of the Barito River is an important ecosystem, yet its utilization patterns by indigenous communities in the area remain relatively limited. The lack of comprehensive data may hinder efforts in documentation, conservation, and the management of biological resources based on local wisdom. This study aims to document the diversity of plant species used by the Dayak Bakumpai community along the banks of the Barito River in Sungai Gampa Village, Barito Kuala Regency, South Kalimantan. The research will be conducted from August to September 2025 using a descriptive exploratory design with both qualitative and simple quantitative approaches. Data will be collected through field observations using an exploration method to record morphological characteristics of roots, stems, leaves, flowers, and fruits. The results identified nine plant species, namely Syzygium aqueanum (water apple), Syzygium polyanthum, Syzygium malaccense (L.) Merr., Psidium guajava (red guava and white guava), Melaleuca cajuputi Powell (large-leaf and small-leaf galam), and Melastoma candidum, with the genus Syzygium dominating in terms of species number. The utilization of these plants by the community includes fruit as a food source, traditional medicine, and essential oil production, demonstrating the close relationship between riparian plant diversity and the socio-economic needs of the local community. This inventory provides baseline data on the riparian flora of the Barito River, which is valuable for biodiversity documentation, vegetation monitoring, and community-based riparian area management.
Pengembangan Kit Isolasi DNA Low-Cost dan Ramah Lingkungan untuk Berbagai Sampel Tirtasari , Ni Luh; Eralita, Norma; Siswanti, Siswanti
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.694

Abstract

Science learning includes the study of concepts as well as practical activities. Practical science learning can run effectively when supported by adequate laboratory facilities and infrastructure. This study aims to develop a low-cost and environmentally friendly DNA isolation kit as an alternative medium for molecular biology practicum that is more affordable and easier to implement in educational laboratories. The kit development utilizes simple materials and equipment that are easily obtained from the surrounding environment, such as enzyme-based liquid detergent, table salt, natural enzyme extracts (pineapple, ginger, papaya leaves, and papain), and ethanol. The research method employed an experimental approach through stages of kit formula design, optimization of lysis buffer composition, and effectiveness testing on various plant and animal tissue samples, namely strawberry, broccoli, chicken liver, and chicken meat. The success parameters were observed through the visualization of DNA threads resulting from precipitation and the measurement of DNA quantity and purity using a spectrophotometer. The results indicate that all kit formulas were able to extract DNA with varying levels of success, where strawberry and chicken liver samples showed the clearest DNA thread visualization and the best color appearance. These findings demonstrate that a locally based, low-cost, and eco-friendly DNA isolation kit is effective as an alternative to commercial kits and has strong potential to support science learning, particularly in genetics and biotechnology, through more practical and economical laboratory activities.
Persepsi Masyarakat Sunda terhadap Pemanfaatan Beragam Tanaman Pekarangan di Desa Solear, Tangerang, Banten Hartati, Sri; Kusumaningrum, Elizabeth Novi
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.698

Abstract

The rapid growth and development of urban communities in the central area of Tangerang Regency, followed by advances in information technology, have indirectly influenced changes in the lifestyles of rural communities in the regency. The purpose of this study was to identify and describe the use of plants growing in the yards of Sundanese communities in Solear Village, Solear District, Tangerang Regency, Banten. This study used a qualitative descriptive method conducted through a survey to collect data on yard plant species and interviews on their utilization by the Sundanese community in the village. The data was analyzed through reduction, grouping, data presentation, and conclusion drawing. Data analysis from yard surveys and interview results was carried out by classifying plant species and patterns of use based on their functions and the local wisdom of the community. The results of the study show that there are 55 species of plants growing in the yards of the Sundanese community in Solear Village, which are used in daily life for six types of benefits, namely as vegetables, kitchen spices, fruits, traditional medicine, ornamental plants, and building materials. This research contributes to ethnobotanical studies by providing systematic data on the use of yard plants, which supports the preservation of traditional knowledge and sustainable management of biological resources in areas affected by urbanization.  
Analisis Kombinasi Bakteri Lactobacillus lactis dan Lactobacillus bulgaricus terhadap Protein dan Lemak Keju Susu Kerbau (Bubalus bubalis) selama Masa Simpan Sari, Linda; Nasution, Rizki Amelia; Selvia, Irda Nila
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.702

Abstract

Buffalo milk (Bubalus bubalis) has a high protein and fat content and lower cholesterol than cow's milk, but this nutritional content causes a relatively short shelf life so that further processing is required, one of which is to make cheese. During the shelf life, the quality of cheese can decrease due to microbial activity, enzymatic reactions, and protein and fat degradation. Lactobacillus lactis and Lactobacillus bulgaricus bacteria produce proteolytic enzymes that can modify the protein and fat content in cheese. This study aims to analyze the effect of the combination of Lactobacillus lactis and Lactobacillus bulgaricus bacteria on the protein and fat content of buffalo milk cheese at different shelf lives. The study used four treatments without repetition with shelf lives of 1, 7, and 14 days. Protein content was analyzed using the Kjeldahl method and fat content using the Soxhlet method. Data on pH, water content, total LAB, protein content, and fat content were analyzed descriptively quantitatively, while organoleptic tests were analyzed using the Kruskal–Wallis test followed by the Mann–Whitney test (p<0.05). The results showed that the combination of bacteria increased protein and fat content as the shelf life increased, with the highest values ??being 47.44% and 32.94%, respectively, accompanied by a decrease in pH and total LAB. In conclusion, the addition of 3% Lactobacillus lactis and 3% Lactobacillus bulgaricus can improve the quality of buffalo milk cheese. This research contributes to the science of food fermentation regarding the role of the combination of Lactobacillus lactis and Lactobacillus bularicus bacteria on the chemical characteristics of buffalo milk cheese during shelf life.
Keanekaragaman Tumbuhan Pewarna Alami dan Pola Pemanfaatannya oleh Masyarakat Suku Aceh Hartono, Adi; Sulaiman, Nanda Fahira; Arifah, Annisyah; Fauziani, Fauziani
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.703

Abstract

The use of plants as natural dyes represents important traditional knowledge of the Acehnese people, closely linked to cultural practices, crafts, and the use of local natural resources. However, modernization and synthetic dyes threaten the continuity of these traditions. This study aimed to examine the diversity of natural dye plants and their utilization patterns within Acehnese communities. A qualitative ethnobotanical approach was applied through field observations, semi-structured interviews with key informants, and documentation of dye-producing plant species. Data were analyzed descriptively by grouping plants based on parts used, colors produced, and forms of utilization. The findings show that various local plant species are used as natural dye sources, with leaves, bark, and fruits being the most commonly utilized parts. Utilization patterns are strongly connected to cultural needs and local wisdom in maintaining environmental balance. The study concludes that ethnobotanical knowledge of natural dyes persists despite modernization, highlighting its importance for preserving local knowledge and promoting environmentally friendly dye alternatives in cultural and applied science contexts.
Analisis Kebutuhan Media Pembelajaran Interaktif Biologi pada Siswa SMA Kelas X Putriyani, Eryzinda Dwigita; Murtini, Iin
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.704

Abstract

Biology learning at the senior high school level requires the support of interactive learning media to help students understand complex and abstract biological concepts. This study aims to analyze the need for interactive biology learning media among tenth-grade students at SMA Negeri 5 Tuban. A quantitative descriptive method was employed, using non-test data collection techniques in the form of questionnaires and teacher interviews. The instrument was developed using a Guttman scale (Yes/No) consisting of 15 items grouped into three aspects: learning media needs, accessibility and ease of use of learning media, and learning motivation and interest. The subjects of this study were tenth-grade students of SMA Negeri 5 Tuban. Data were analyzed descriptively to determine the level of students’ needs for interactive biology learning media. The results showed that the learning media needs aspect reached 92%, accessibility and ease of use reached 85%, and motivation and learning interest reached 99%, with an overall average of 92%, categorized as a very high level of need. These findings indicate that students have a very high demand for interactive biology learning media, highlighting the importance of developing interactive biology learning materials that align with students’ needs and characteristics.
Identifikasi dan Patogenisitas Agen Bakteri Penyebab Penyakit Busuk Batang pada Buah Naga (Hylocereus sp.) Fahmi, Riziq Ilham Nur; Cahlia, Uci; Wibowo, Risky Hadi; Sipriyadi, Sipriyadi
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.705

Abstract

The increasing intensification of dragon fruit (Hylocereus sp.) cultivation is followed by an increase in disease attacks that have the potential to reduce plant productivity. This study aims to isolate, identify, and test the pathogenicity of bacteria that cause dragon fruit stem rot in Pematang Donok Village, Kepahiang, Bengkulu. Bacterial isolation was carried out from disease-infected stem tissue, followed by isolation purification. Bacterial identification was carried out based on colony morphology and Gram staining, while pathogenicity and hemolysis tests were carried out on potatoes and blood agar media, respectively. The data were analyzed descriptively qualitatively through observation of colony morphological characteristics and Gram staining, as well as pathogenicity tests on potatoes and hemolysis tests on blood agar. The isolation results obtained two bacterial isolates, namely isolates BBNK1 and BBNK2 with different colony morphology characteristics. Both isolates are Gram-positive bacteria with bacillus and coccus cell shapes. Pathogenicity tests on potatoes showed that both isolates were able to cause soft rot symptoms such as tissue softening, discoloration, and mucus formation compared to the control. Hemolysis tests showed that only isolate BBNK1 showed hemolytic activity with the formation of a clear zone around the isolate. The findings of this study offer foundational data to support future research on molecular identification and the development of more environmentally friendly and sustainable disease management strategies.

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