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Arif Rahman Hikam
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BioEksakta : Jurnal Ilmiah Biologi Unsoed
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 27148564     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
Jurnal BioEksakta menerbitkan artikel ilmiah hasil penelitian dalam bidang biologi umum termasuk Ekologi dan konservasi, Taksonomi dan Struktur, Biogeografi, Evolusi, Biodeversitas, Fisiologi dan Reproduksi, Biologi sel, Biologi Molekuler dan Genetika.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 16 Documents
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Search results for , issue "Vol 3 No 2 (2021): BioEksakta" : 16 Documents clear
FITOREMEDIASI MENGGUNAKAN KAYU APU (Pistia stratiotes) UNTUK MENURUNKAN KADAR COD LIMBAH CAIR TEKSTIL Utami Sari Dewi; Slamet Santoso; Elly Proklamasiningsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4375

Abstract

Waste water treatment is needed to avoid the impact of pollution to the aquatic environment. Techniques for treating waste water, among others, are through phytoremediation using the help of water lettuce (Pistia stratiotes).This research aims to determine the effect of the percent covering area of water lettuce, the length of exposure time, and interaction of both in reducing levels COD, and to determine the percent of covering area, the length of exposure time, and interaction of both that is the most efficient in reducing The result of this research showed that the percent covering area, and the length of exposure time had a very significant effect of decreasing levels of COD. The most efficient percent covering area in reducing COD was in treatment of 75%. The most efficient length of exposure time in reducing COD was in treatment 9 days. The most efficient interaction of both in reducing COD levels was at treatment of 100% coverage area with exposure time of 9 days.
PENAMBAHAN IAA DAN BAP TERHADAP PERTUMBUHAN KALUS EKSPLAN DAUN ANGGREK Coelogyne pandurata Lindl. Johanes De Britto Bintan Cahyo Adi; Kamsinah Kamsinah; Lucky Prayoga
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4255

Abstract

Anggrek Coelogyne pandurata Lindl atau anggrek hitam merupakan anggrek endemik dari Kalimantan. Anggrek ini mempunyai keunikan yakni mempunyai sepal berwarna hijau serta labelum yang berwarna hitam. Perbanyakan secara alami tingkat keberhasilannya sangat kecil maka perlu di lakukan kultur in vitro. Salah satu cara kultur in vitro adalah kultur menggunakan eksplan daun untuk memacu pertumbuhan kalus. Pertumbuhan kalus dapat dipacu dengan auksin (IAA) dan sitokinin (BAP). Perpaduan antara konsentrasi auksin dan sitokinin yang seimbang diharapkan bisa memacu pertumbuhan kalus. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan IAA dan BAP terhadap pertumbuhan kalus anggrek C. pandurata Lindl dan mengetahui interaksi IAA dan BAP yang terbaik terhadap pertumbuhan kalus anggrek C. pandurata Lindl. Metode penelitian dilakukan secara eksperimental dengan rancangan acak lengkap menggunakan pola faktorial , faktor 1 kosentrasi IAA dengan 4 taraf : 0, 1, 2, 3 mg/L. Dan faktor ke 2 konsentrasi BAP dengan 4 taraf perlakuan : 0, 1 , 2, 3 mg/L, sehingga ada 16 perlakuan , dan masing-masing perlakuan diulang 3 kali sehingga ada 48 unit percobaan. Parameter yang diamati adalah : waktu munculnya kalus, tebal kalus, jenis kalus, berat kalus dan presentasi terbentuknya kalus. Data yang diperoleh dianalisis menggunakan ANOVA dan dilakukan uji lanjut DMRT dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan pemberian IAA dan BAP dapat mempengaruhi pertumbuhan kalus eksplan daun anggrek Coelogyne pandurata Lindl. Pengaruh secara interaksi terdapat pada wantu munculnya kalus, tebal kalus dan Prosentase hidup kalus. Serta mampu memacu perkembangan kalus ke arah proliferatif. Sedangkan pemberian IAA secara mandiri dapat memacu penebalan kalus. Interaksi yang terbaik dalam pertumbuhan kalus eksplan daun anggrek Coelogyne pandurata Lindl. adalah A2B2 (IAA 2 mg/L dan BAP 2 mg/L) dan A2B3 (IAA 2 mg/L dan BAP 3 mg/L).
Keanekaragaman Serangga pada Tanaman Cabai Merah Di Desa Kubangwungu Kecamatan Ketanggungan Kabupaten Brebes Dwi Yuliani; Devi Ayu Susilowati; Anton Maulana Ibrahim
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4278

Abstract

The increasing number of population and the growing food industry that requires raw material for red chilies have led to an increase in demand for red chilies. The research was conducted using a survey method which was carried out from February 2021 to April 2021. Sampling of insect diversity was carried out on 1,000 m2 of red chili plantations. The land for the red chili plants was determined by 10 points randomly. Each sampling point consisted of 2 pitfall traps, 1 light trap, and 1 insect net with 4 replications. Insect sampling was carried out when the chili plants began to enter the generative period (44-50 days after planting) for 6 consecutive days, in this phase the insect pests began to be seen in many chili plants. The results of the sampling were then identified using the J. Borror (1992) determination key book. Analysis of insect diversity data was carried out by calculating the diversity index (diversity) based on Magurran (1988) using the Shannon-Evenness Index and the Shannon-Evenness Index. The results showed that 28 species consisted of the order hemiptera, coleopteran, prthoptera, diptera, hymenoptera, odonata, mantodea, and Lepidoptera. The results showed that the diversity index of insect pollinators on red chili agricultural land using the pit fall method (H´ = 2.874) was lower than the light (H '= 3.029) and netting methods (H' = 3.0159), while the evenness index on land The pit fall method of red chili farming (E = 0.8627) was lower than the light method (E = 0.909) and netting method (E = 0.9481). Keywords: diversity, insects, red chilies
KUALITAS BIJI DAN KARAKTER AGRONOMI PADI HITAM (Oryza sativa L.) LOKAL PEKALONGAN DENGAN PENAMBAHAN PAKLOBUTRAZOL DAN GIBERELIN Endang Triyani Prihantari; Triani Hardiyati; Siti Samiyarsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4240

Abstract

Black rice (Oryza sativa L.) is a rice variety that has many additional functions and is used as a functional food. However, black rice has a longer harvest period and has a higher apparent stem height than other types of rice so that black rice plants are easy to break. This will reduce the quality of rice plants. One of the methods that can be done to overcome this problem is treating by paclobutrazol and gibberellin. The combination of paclobutrazol and gibberellin is expected to provide better rice growth and improve the quality of rice seeds. The purpose of this study was to determine the effect of the addition of paclobutrazol and gibberellin on seed quality, palea-lemma size and agronomic character of local Pekalongan black rice (Oryza sativa L.). The research was conducted experimentally using a completely randomized design (CRD) of factorial treatment pattern. The first factor was paclobutrazol with 4 levels of concentration (0 ppm, 100 ppm, 200 ppm and 300 ppm) applied once at 4 weeks of plant age and the second factor was gibberellin with 4 levels of concentration (0 ppm, 100 ppm, 200 ppm and 300 ppm ) applied once at the age of 6 weeks of plants, so that are 48 combinations with each treatment was replicated 3 times. The parameters observed included plant height, leaf width, flowering age, harvest age, palea-lemma size, weight of 100 seeds, protein and amylose content. The data obtained were analyzed using the ANOVA test at the 95% confidence level, and continued with the Honestly Significant Difference (HSD) test at an error rate of 5%.The results showed that the interaction between paclobutrazol and gibberellin was not significant, but treatment of 300 ppm paklobutrazol showed the best results in plant height inhibition, while the treatment of paclobutrazol 200 ppm was the best concentration to stimulate flowering and harvesting age. Treatment of gibberellin 100 ppm was found the best concentration to increase leaf width while a concentration of 300 ppm proved to give the best results for palea-lemma size, amylose content, protein content and weight of 100 seeds per plant.
Karakteristik Morfologi Post Larva Udang Penaeus dan Metapenaeus dari Kawasan Timur Segara Anakan Cilacap Harditya Firdhaus; Dian Bhagawati; Kusbiyanto Kusbiyanto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4554

Abstract

Information about the presence of post larvae in nature could indicate the specific presence of the sites used for growth and development of larvae and post larvae. This could be an alternative option to determine the diversity of shrimp in an area with the presence of post larvae. Research about species richness of post larvae phase of shrimp based on morphology characters, especially in the eastern region of Segara Anakan Cilacap is still rarely done, so it is necessary to collected basic data on the morphological characters of post larvae phase of shrimp. The purpose of this research is to knew species richness and morphological description post larvae phase of shrimp in the eastern region of Segara Anakan, Cilacap Regency, Central Java. The method used survey with a random sampling technique from the collection of the Laboratory of Animal Taxonomy, Faculty of Biology, Jenderal Soedirman University. The variables in this research were morphological performance, standar morphometric, and meristic of post larvae shrimps. The parameters observed were characters of morphological description on each post larvae phase of shrimp, ratio standard morphometric, and meristic calculation. Species richness, morphological performance, standard morphometric, and meristic data were analyzed descriptively. The results obtained were three species of post larvae of Family Penaeidae namely Penaeus indicus, P. merguiensis, and Metapenaeus ensis.
POTENSI EKSTRAK ETIL ASETAT Coprinus comatus TERHADAP KADAR SGOT DAN SGPT PADA TIKUS PUTIH MODEL DIABETES Feryawan Feryawan; Nuniek Ina Ratnaningtyas; Nuraeni Ekowati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4238

Abstract

Coprinus comatus or shaggy in cap mushroom contain bioactive compounds including flavonoids, alkaloids, terpenoids, tocopherols, comatin, and ergotionin which function as antioxidants and antidiabetic. Diabetes mellitus is a chronic disease associated with impaired insulin secretion or decreased insulin sensitivity which causes blood glucose levels to rise above normal (hyperglycemia). Hyperglycemia conditions can causes oxidative stress and increase Reactive Oxygen Species (ROS), to trigger lipid peroxidation which damages the pancreas, kidneys and liver. One way to determine liver damage is by measuring the increase in liver enzyme levels of Serum Glutamate Oxaloacetate Transaminase (SGOT) and Serum Glutamate Pyruvate Transaminase (SGPT) in the bloods. The aims in this study is determine the effect of administration and determine the effective doses of ethyl acetate C. comatus extract with different doses on the levels of SGOT and SGPT in the bloods of diabetic rats. The results showed that C. comatus ethyl acetate extract could reduce SGOT and SGPT levels as well as streptozotocin-induced rat bloods glucose levels. Ethyl acetate C. comatus extract with doses 500 mg / kg BW is the most effective doses in reducing SGOT and SGPT levels in the bloods of streptozotocin-induced rats with a mean SGOT level of 57,96 ± 1,58 U/L and SGPT levels of 29,67 ± 3,56 U/L, and the percentage reduction in SGOT and SGPT levels compared to negative controls respectively was 43,15% and 68,70%, and blood glucose was 19,62%.
KAJIAN ETNOBOTANI TANAMAN Curcuma longa L. DAN Curcuma zanthorrhiza ROXB. OLEH MASYARAKAT BOGOR JAWA Malia Ulfah; Wardah Wardah; Rusmadi Rusmadi; Muhammad A'tourrohman
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4287

Abstract

The community of Bogor, West Java are a group of people who still apply the traditional pattern of life. The use of turmeric plants (Curcuma longa L.) and temu lawak (Curcuma zanthorrhiza Roxb.) by local people is still ongoing today. This research was conducted to determine the economic value of turmeric and temulawak for the people of Bogor, the use and use of turmeric and temu lawak plants, and the role of ethnobotany for the development of science. The method used in this study is data collection techniques in the form of literature studies, observations, interviews, and documentation. The results show that turmeric and temu lawak have economic potential for the people of Bogor, West Java. Its uses include medicinal plants, food additives and as vegetables. How to use it is to be mashed, cut and consumed directly. Revitalizing community knowledge about the use of plants around is very important for the development of science.
Prevalensi dan Intensitas Tungau Parasit pada Kucing Peliharaan yang Diperiksakan di beberapa Klinik Hewan Purwokerto Amelia Nurma Hidayah; Bambang Heru Budianto; Hery Pratiknyo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4564

Abstract

This study aimed to determine the prevalence and intensity of parasitic mites in domestic cats at several veterinary clinics in Purwokerto. This research was conducted by cross sectional method using random sampling technique at three veterinary clinics in Purwokerto City. The identification of parasitic mites was analyzed descriptively, the data on the mites found were then analyzed using the prevalence and intensity formulas. The prevalence and intensity figures obtained were then compared with the prevalence and intensity category tables. The results of the study reported that the total prevalence of parasitic mites was 85%, which was included in the category of prevalence usually with moderate infestation, with a total intensity of parasitic mites of 196.7 which included in the category of very severe intensity. Species of parasitic mites found include Otodectes cynotis, Notoedres cati and Lynxacarus radovskyi.
POTENSI EKSTRAK ETIL ASETAT Coprinus comatus TERHADAP KADAR SGOT DAN SGPT PADA TIKUS PUTIH MODEL DIABETES Feryawan, Feryawan; Ratnaningtyas, Nuniek Ina; Ekowati, Nuraeni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4238

Abstract

Coprinus comatus atau jamur paha ayam mengandung senyawa bioaktif antara lain flavonoid, terpenoid, comatin, tokoferol, dan ergotionin yang berfungsi sebagai antioksidan dan antidiabetes. Diabetes mellitus merupakan penyakit kronis yang berkaitan dengan gangguan sekresi insulin atau menurunnya sensitivitas insulin yang menyebabkan kadar glukosa dalam darah meningkat melebihi normal (hiperglikemia). Hiperglikemia dapat menyebabkan stres oksidatif dan meningkatkan Reactive Oxygen Species (ROS) sehingga memacu terjadinya peroksidasi lipid yang merusak pankreas, ginjal, dan hati. Salah satu cara untuk mengetahui kerusakan hati dengan mengukur kadar enzim hati Serum Glutamat Oksaloasetat Transaminase (SGOT) dan Serum Glutamat Piruvat Transaminase (SGPT) yang meningkat dalam darah. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian dan menentukan berapakah dosis yang efektif ekstrak etil asetat C. comatus dengan dosis yang berbeda terhadap kadar SGOT dan SGPT dalam darah tikus putih model diabetes. Hasil penelitian menunjukkan bahwa pemberian ekstrak etil asetat C. comatus dapat menurunkan kadar SGOT dan SGPT serta kadar glukosa darah tikus putih yang diinduksi streptozotocin. Ekstrak etil asetat C. comatus dosis 500 mg/kg BB merupakan dosis yang paling efektif dalam menurunkan kadar SGOT dan SGPT dalam darah tikus yang diinduksi streptozotocin dengan rerata kadar SGOT sebesar 57,96 ± 1,58 U/L dan SGPT sebesar 29,67 ± 3,56 U/L, serta presentase penurunan kadar SGOT dan SGPT yang dibandingkan kontrol negatif masing-masing sebesar 43,15% dan 68,70%, serta glukosa darah sebesar 19,62%. Kata kunci : Coprinus comatus, diabetes mellitus, SGOT, SGPT
KUALITAS BIJI DAN KARAKTER AGRONOMI PADI HITAM (Oryza sativa L.) LOKAL PEKALONGAN DENGAN PENAMBAHAN PAKLOBUTRAZOL DAN GIBERELIN Prihantari, Endang Triyani; Hardiyati, Triani; Samiyarsih, Siti
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4240

Abstract

Padi hitam (Oryza sativa L.) adalah varietas padi yang memiliki banyak fungsi tambahan dan dijadikan pangan yang fungsional. Namun, padi hitam memiliki masa panen yang lebih lama dan memiliki batang yang lebih tinggi dibandingkan padi jenis lain sehingga tanaman padi hitam rentan mengalami rebah. Hal ini akan menurunkan kualitas tanaman padi. Salah satu metode yang dapat dilakukan untuk mengatasi hal ini dengan memberi perlakuan paklobutrazol dan GA3. Perpaduan antara paklobutrazol dan GA3 diharapkan dapat memberikan pertumbuhan padi yang lebih baik dan meningkatkan kualitas biji padi. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan paklobutazol dan GA3 terhadap kualitas biji, ukuran palealemma dan karakter agronomi padi hitam kultivar Pekalongan. Penelitian dilakukan secara eksperimental menggunakan rancangan acak lengkap (RAL) pola perlakuan faktorial. Faktor pertama yaitu paklobutrazol dengan 4 taraf konsentrasi (0 ppm, 100 ppm, 200 ppm dan 300 ppm) diberikan 1 kali pada usia tanaman 4 minggu dan faktor kedua yaitu GA3 dengan 4 taraf konsentrasi (0 ppm, 100 ppm, 200 ppm dan 300 ppm) diberikan 1 kali pada usia tanaman 6 minggu, sehingga terdapat 48 kombinasi dengan masing-masing perlakuan diulang sebanyak 3 kali. Parameter yang diamati meliputi tinggi tanaman, lebar daun, umur berbunga, umur panen, ukuran palea-lemma, berat 100 biji, kandungan protein dan amilosa. Data yang diperoleh dianalisis dengan uji ANOVA pada tingkat kepercayaan 95%, dilanjutkan dengan uji Beda Nyata Jujur (BNJ) pada tingkat kesalahan 5%.Hasil penelitian menunjukkan bahwa interaksi antara paklobutrazol dan GA3 tidak nyata namun perlakuan paklobutrazol mandiri konsentrasi 300 ppm memberikan hasil terbaik untuk penghambatan tinggi tanaman, sedangkan pemberian paklobutrazol konsentrasi 200 ppm merupakan konsentrasi terbaik dalam memacu umur berbunga dan umur panen. Pemberian GA3 100 ppm adalah konsentrasi terbaik untuk menambah lebar daun sedangkan pada konsentrasi 300 ppm mampu memberikan hasil terbaik untuk ukuran palea-lemma, kandungan amilosa, kandungan protein dan berat 100 biji per tanaman. Kata kunci: GA3, padi hitam kultivar Pekalongan (Oryza sativa L.), paklobutrazol

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