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HAYATI Journal of Biosciences

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Published by Institut Pertanian Bogor

ISSN : 19783019 EISSN : 20864094 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.

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Articles 9 Documents

Search results for , issue "Vol. 19 No. 1 (2012): March 2012" : 9 Documents clear

The effect of cholesterol on the survival and development of larval mud crab Scylla serrata were examined by feeding larvae with Artemia enriched with different level of cholesterol. Artemia enriched with four stated levels of cholesterol i.e., 0, 5, 10, and 20 ul/l (Chol 0, 5, 10, and 20). All treatments were mixed with DHA70G at 25 ul/l. All the oil was adjusted to 100 ul/l by adding the oleic acid. Survival rate, intermolt period, and carapace width at the fisrt crab stage of mud crab larvae MUHAMMAD AGUS SUPRAYUDI; TOSHIO TAKEUCHI; KATSUYUKI HAMASAKI
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.1.1

The effect of cholesterol on the survival and development of larval mud crab Scylla serrata were examined by feeding larvae with Artemia enriched with different level of cholesterol. Artemia enriched with four stated levels of cholesterol i.e., 0, 5, 10, and 20 ul/l (Chol 0, 5, 10, and 20). All treatments were mixed with DHA70G at 25 ul/l. All the oil was adjusted to 100 ul/l by adding the oleic acid. Survival rate, intermolt period, and carapace width at the fisrt crab stage of mud crab larvae fed Chol 0, 5, and 10 were higher compared to that of Chol 20 (P < 0.05). We suggest that free sterol contained in Artemia at 1.37% was harmful to the growth performance of mud crab larvae. This study suggests that mud crab larvae required at least 0.61% cholesterol for maintaining good survival and development and therefore no need to enrich Artemia by cholesterol for the practical purpose.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.1.11

Microalgae concentrates (paste) can be used as an alternative feed to replace live microalgae for aquaculture due to its nutritional value and convenience. However, the clumping of cells and negative buoyancy of algae concentrate can affect bivalve culture as bivalves only capture particles in suspension and ingest a certain size range of particles. This study investigated the effect of shaking and blending treatments on the preparation of food suspensions prepared from algae concentrates (Isochrysis and Pavlova). The results indicated that the higher the shaking time (5, 10, and 15 times) or blending time (10, 30, and 60 seconds), the smaller was the diameter of the resulting algae particles. Moreover, the greater the volume of algae concentrate used in preparation, the larger the diameter of algae particles produced. Shaking may be the best option because it is cheaper and simpler. However, all the treatments provided a suitable particle size range for ingestion by bivalves.

Isolation and Identification of Transforming Growth Factor β from In Vitro Matured Cumulus Oocyte Complexes . WIDJIATI; ARIEF BOEDIONO; SUTIMAN BAMBANG SUMITRO; AUCKY HINTING; . AULANI’AM; TRINIL SUSILOWATI
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

Transforming growth factor-β (TGF-β) is a two-chain polypeptide with molecular weight of 25 kDa which takes significant role in the steroidogenesis process. In the ovarian oocyte in particular, TGF-β has an important role in regulating reproductive function. TGF-β represents a key intrafollicular protein that regulates follicle development and aromatization process. The purpose of this research was to characterize and identify a protein fraction of TGF-β from the bovine isolated oocytes, which is synthesized during in vitro oocyte maturation process. Oocytes were collected from follicles with diameter of 3-8 mm. Oocytes were then matured in TCM 199 media supplemented with 5 μg/mg LH, 3% BSA, and 50 μg/ml gentamicin sulfate, and cultured in CO2 incubator (5%, 38.5 oC) for 20 hours. TGF-β receptors were identified immunohistochemically. Characteristics of the TGF-β protein were determined using SDS PAGE and TGF-β specification was tested using Western Blotting. The results showed that TGF-β receptors were identified and found in cumulus oocyte complexes (COCs). TGF-β protein was isolated from bovine oocytes with molecular weight 25 kDa and it was identified by Western blotting methods in the same molecular weight.

The Occurrence of Hybrid in Nepenthes hookeriana Lindl. from Central Kalimantan can be Detected by RAPD and ISSR Markers KUSUMADEWI SRI YULITA; MUHAMMAD MANSUR
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

Nepenthes spp. (Nepenthaceae) is one of the most popular ornamental plants in Southeast Asia. There are 97 species of Nepenthes to which 64 are found in Indonesia with the center of its diversity located in Borneo. N. x hookeriana was hypothesised to be a natural hybrid between N. ampullaria and N. rafflesiana on the basis of morphological characters. Several variants of each species were also known. This present study aimed to detect the occurrence of hybrid within N. x hookeriana ‘spotted’ and ‘green’ variant using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR). Five RAPD primers and three ISSR primers were used to amplify total DNA genome and produced 83 polymorphic bands ranging in size from 300-1700 bp. Clustering analysis was performed based on RAPD and ISSR profiles using the UPGMA method. The genetic similarity of the combined markers range between 0.30-0.75 indicating a narrow range of genetic similarity among the accessions. Results from cluster analyses suggested that N. x hookeriana was indeed a hybrid between N. ampullaria and N. Rafflesiana, however it was genetically more similar to N. raflessiana.

Soy Germ Protein With or Without-Zn Improve Plasma Lipid Profile in Metabolic Syndrome Women HERY WINARSI; SIWI PRAMATAMA MARS WIJAYANTI; AGUS PURWANTO
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

The aim of this research was to determine the effect of soy germ protein on lipid profile of metabolic syndrome (MetS) patients. Respondents were 30 women with criteria, i.e. blood glucose level > normal, body mass index > 25 kg/m2, hypertriglyceridemia, low cholesterol-HDL level, 40-65 years old, living in Purwokerto, and signed the informed consent. The project was approved by the ethics committee of the Medical Faculty from Gadjah Mada University-Yogyakarta. Respondents were divided into three randomly chosen groups consisting of ten women each. The first, second, and third groups were treated, respectively, with milk enriched soy germ protein plus Zn, milk enriched soy germ protein (without Zn), and placebo for two months. Blood samples were taken at baseline, one and two months after observation. Two months after observation the groups consuming milk enriched with soy germ protein, both with or without Zn, had their level of cholesterol-total decrease from 215.8 to 180.2 mg/dl (P = 0.03), triglyceride from 240.2 to 162.5 mg/dl (P = 0.02), and LDL from 154.01 to 93.85 mg/dl (P = 0.03). In contrast, HDL increased from 38.91 to 49.49 mg/dl (P = 0.0008). In conclusion, soy germ protein can improve lipid profile, thus it can inhibit atherosclerosis incident.

Toxicity Study of Antidiabetics Functional Drink of Piper crocatum and Cinnamomum burmannii MEGA SAFITHRI; SEDARNAWATI YASNI; MARIA BINTANG; ANNA SETIADI RANTI
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

Piper crocatum and Cinnamomum burmannii formulations is known to be a new diabetes functional drink. Thus, its toxicological profile needs to be studied. At present, the formulation was evaluated for the repeated dose toxicity study. The Sprague dawley albino rats were treated with P. crocatum and C. burmannii formulations (0, 630, 1260, and 1890 mg/kg) and administered orally for a period of 28 days in albino rats. The effects on body weight, food and water consumption, organ weight, hematology, clinical biochemistry as well as histology were studied. There were no significant differences in the body weight, organ weights and feeding habits between control and treated animals. Hematological analysis showed no marked differences in any of the parameters examined in either the control or treated groups. There were no significant changes that occurred in the blood chemistry analysis including glucose, cholesterol, triglycerides, creatinine, SGPT, and SGOT in experimental animals. Pathologically, neither gross abnormalities nor histopathological changes were observed. The formulation of P. crocatum and C. burmannii was found safe in repeated dose toxicity studies.

Physiological and Biochemical Responses to Aluminum Stress in the Root of a Biodiesel Plant Jatropha curcas L. RADITE TISTAMA; UTUT WIDYASTUTI; DIDY SOPANDIE; AKIHO YOKOTA; KINYA AKASHI; . SUHARSONO
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

We investigated J. curcas responses to aluminum stress, histochemically and biochemically. Histochemical stainings were observed to analysis aluminum accumulation, lipid peroxidation and the loss of plasma membrane integrity on the surface and tissue of the root apex. Enzymatic analysis was conducted to measure malate content in leaf, root and malate efflux in the medium. We used M. malabathricum as a comparison for Al-tolerance plant. J. curcas root elongation was inhibited by 0.4 mM AlCl3, while M. malabathricum root elongation was inhibited by 0.8 mM AlCl3 treatment. Inhibition of root elongation has high correlation with Al accumulation in the root apex, which caused lipid degradation and cell death. Generally, malate content in J. curcas leaf and root was higher than that in M. malabathricum. In the contrary malate efflux from the root into the medium was lower. J. curcas root has a different pattern compared to M. malabathricum in malate synthesis and malate secretion when treated with a different Al concentration. We categorized J. curcas acc IP3 as more sensitive to aluminum than M. malabathricum.

Morphological Characters and Histology of Pheretima darnleiensis ANDY DARMAWAN; RIKA RAFFIUDIN; TRI HERU WIDARTO
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

Pheretima darnleiensis is a native earthworm of Southeast Asia, India, and Japan. Although it is commonly found in Indonesia, the earthworm has never been studied well. This study was aimed to examine the morphological characters and structure of its several organs for an identification purpose, which is important for the earthworm culture. Earthworms were collected in a plot of 55-150 x 55-150 cm width and 20 cm depth at Bogor Agricultural University in Darmaga and Baranangsiang Campuses by hand sorting method. Examinations were carried out on its external as well as internal characters. The histology of the organs was studied using paraffin method. The observed characters on P. darnleiensis were the presence of prostate gland, one pair of male pores on segment XVIII, a cylindrical body with perichaetine setae, caeca on segment XXVII, copulatory pouches without diverticula and stalked glands, bithecal spermatheca with nephridia, and the first spermathecal pore on segment 4/5. In addition, other characters found on P. darnleiensis were the presence of an annular clitellum on segment XIV-XVI, an epilobus prostomium with open base, approximately 40 single pointed setae on segment XIII, one midventral female pore on segment XIV, one pair of lateroventral male pores on segment XVIII, four pairs of lateroventral spermathecal pores on segment 4/5, 5/6, 6/7, 7/8, and the first middorsal dorsal pore on segment 12/13. The histology of P. darnleiensis showed basic structure as found in other earthworms.

Anti Lithiasis Activity of Avocado (Persea americana Mill) Leaves Extract in White Male Rats IETJE WIENTARSIH; RINI MADYASTUTI; BAYU FEBRAM PRASETYO; ANGGARA ALDOBRATA
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

In Indonesia, avocado leaves have been used as traditional medicines for diureticum to cure urolithiasis. This research was to determine anti lithiasis activity of avocado leaves (Persea americana Mill) extract on white male rats nefrolithiasis model induced by ethylene glycol. Ethanol extraction method was used to get extract of avogadro leaves. Twenty adult male white rats were divided into 4 different induction treatments i.e. aquadest, ethylene glycol 0.75% and ammonium chloride 2%, and extract of avocado leaves with different levels of 100 and 300 mg/kg bw respectively. Their body weight was measured daily to determine their growth ratio. And at the end of the trial, the kidney was analyzed its calcium level and inhibitory activity to formation of calcium oxalate crystals. The results showed that the amount of calcium level in the kidney of rats treated with extract of avogadro leaves was significantly decreased than that of rats treated with ethylene glycol 0.75% and ammonium chloride 2% (P < 0.05). The extract avocado leaves as a herbal remedy can be recommended as a phytotherapeutic agent especially for preventive action for urolithiasis diseases.

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