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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 21 Documents
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Search results for , issue "Vol. 30 No. 3 (2023): May 2023" : 21 Documents clear
Leclercia adecarboxylata C12, The Newly Isolated Cellulose-degrading Bacteria from Indonesian Coffee Pulp Agustin Krisna Wardani; Ajeng Astrini Brahmanti; Erryana Martati
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.588-595

Abstract

Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Java, Indonesia. Fifty isolates were obtained, and thirty-three isolates showed hydrolyzing zone on Carboxy Methyl Cellulose agar plates after Congo-Red staining. The highest specific CMCase activity was observed by isolates C12, identified as Leclercia adecarboxylata based on 16S-rRNA gene sequence analysis. SDS-PAGE of Leclercia adecarboxylata C12 cellulase revealed two bands with a molecular mass of 95.49 and 81.28 kDa, respectively. Activity gel analysis showed the cellulolytic ability of Leclercia adecarboxylata C12 cellulase by clear zone formation. The optimal CMCase activity was achieved at 50°C and pH 9, and the activity retained 47% of its initial activity after incubation at 50°C for 90 minutes. The purified enzyme remains stable from pH 5 to 10, with 77% of its maximum activity. The activity of CMCase was stimulated by the presence of K+, Ca2+, Mg2+, and Fe3+, while SDS and EDTA reduced its activity. The current study shows that the thermostable-alkalophilic cellulase produced by Leclercia adecarboxylata C12 is very promising for industrial applications.
Phytotoxic and Cytotoxic Polyketides Produced by Fungal Endophytes Isolated from Psidium guajava Tendy Oktriawan; Nanang Ariefta; Tri Joko Raharjo; Endang Astuti; Takuya Koseki; Yoshihito Shiono
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.473-479

Abstract

Endophytic fungi reside within their host plants with no obvious symptoms. They have been proven as abundant sources of new bioactive compounds with diverse structures. In our continuous search of the new compound from endophytic fungi, a new compound, 5,7-dihydroxy-3(R)-methylphthalide (1), was isolated from Xylaria brevipes PGR1, and three known compounds, (–)-altenuene (2), alternariol (3), and altertoxin I (4), were isolated from Alternaria alternata D-8. Both endophytic fungi were isolated from the branch of an Indonesian medicinal plant, Psidium guajava. Their structures were elucidated using extensive spectroscopy analyses, including 1D/2D NMR, HRESITOFMS, and data comparisons with the literature. Compounds 1–3 exhibited phytotoxicity at IC50 (µg/ml) values of 4.6, 23.07, and 77.28, respectively. Additionally, 1–4 showed significant cytotoxicity in the brine shrimp lethality test (BSLT), with mortality rates at 75%, 50%, 90%, and 80% at the concentration of 0.19 μg/ml, respectively.
Enhancing Solubility of Recombinant Plasmodium Lactate Dehydrogenase (pLDH) Using Combination of Cold-Inducible Expression System and Cold-Stirred Bioreactor Alimuddin; Muhamad Ali; Sahrul Alim; Muhamad Amin
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.561-566

Abstract

A major drawback associated with an expression of a high-level Plasmodium Lactate Dehydrogenase (pLDH) using Escherichia coli is the low solubility due to the formation of an inclusion body (IB). This study aimed to develop a suitable protocol for enhancing the solubility of pLDH expressed in E. coli. Firstly, a pLDH-encoding gene was amplified from the blood sample of malaria-infected patients and ligated into pBlueScript II KS+ for sequencing. Afterward, the pLDH gene was digested and cloned into pColdTF for expression. The recombinant plasmid was transformed into the E. coli BL21 (DE3) RIPL Codon Plus Strain. Then, the bacterial host was initially cultured at 37°C until reaching optical density (OD) at 600 nm: 0.5. Thereafter, the growth temperature was lowered to 15°C, followed by the addition of 0.1 mM IPTG into the culture medium for inducing pLDH expression. Thereafter, the bacterial hosts were cultured in a cold-stirred bioreactor (15°C). The result showed that a combination of the low culture conditions (15°C) and a low amount of IPTG increased the solubility of pLDH. This result suggests that this protocol can be a convenient method for generating high-quality recombinant protein using the E. coli system.
Expression of SARS-CoV-2 Nucleocapsid (N) Recombinant Protein Using Escherichia coli System Rizki Aulia Ansari; Uus Saepuloh; Silmi Mariya; Yuliana; Rachmitasari Noviana; Irma Herawati Suparto; Huda Shalahudin Darusman
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.445-450

Abstract

One of the main antigen that can be used for serological testing is the nucleocapsid (N) which is the most abundant viral-derived protein in SARS-CoV-2 where this virus can cause COVID19 disease. The aim of this study was to develop the SARS-CoV-2 N recombinant protein using Escherichia coli expression system. A total of 1,089 nucleotides encoding 362 amino acids of SARS-CoV-2 N was cloned to pET-14b vector. The plasmid then expressed in E. coli BL21 (DE3) and induced with 1.0 mM IPTG (Isopropyl-β-d-1-thiogalactopyranoside). The cell was harvested using denaturation lysis buffer due to inclusion body formation of SARS-CoV-2 N protein. Dialysis processed and concentrated using PEG-6000 resulted 0.992 mg/ml protein yield. Analysis of SARS-CoV-2 N recombinant protein using SDS-PAGE technique showed approximately 37.0 kDa specific band target protein. Application of this SARS-CoV-2 N recombinant protein to vaccinated and non-vaccinated antibody serum samples using ELISA technique indicated the significant result of optical density mean at 0.603 and 0.135, respectively. This study revealed that the production of SARS-COV-2 N recombinant protein could be carried out in E. coli expression system under denatured conditions, therefore the methods are more effective in producing the protein as a basic material in immuno-diagnostic assay.
Phytoremediation of Acid Mine Drainage with Melaleuca cajuputi, Nauclea orientalis, and Vetiveria zizanioides in Floating Treatment Wetland Fitri Arum Sekarjannah; Irdika Mansur; Zaenal Abidin; Anas Miftah Fauzi
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.491-499

Abstract

The formation of acid mine drainage (AMD) is a common environmental problem in the mining industry. Its passive management through wetland construction has gained more consideration in recent years. However, the application in the field is constrained by the large area and relatively shallow depth. Indonesia has no passive technology to neutralize AMD in deep water. One solution is to apply a floating treatment wetland (FTW) system. Therefore, this study aimed to determine the ability of several hyperaccumulator plants, such as Melaleuca cajuputi, Nauclea orientalis, and Vetiveria zizanioides, to neutralize AMD using a floating system by conducting FTW trials. The method used was a Completely Randomized Design (CRD) with 3 treatments and control/without plants. Each treatment had 3 replications, resulting in 12 experimental units. The results showed that the FTW with or without plants could increase pH and decrease dissolved Mn by 75.31-90.74%. Heavy metals were chelated by organic matter, absorbed by plants, and deposited in the form of metal sulfides. The results also indicated that besides having a positive effect on pH and heavy metal reduction, the organic-based floating wetland increased biological oxygen demand (BOD) from 61.08-79.71%.
Evaluation of the Community Structure Leafminer Fly, Liriomyza spp. (Diptera: Agromyzidae) and Their Parasitoids on Various Host Plant Families in Bali Province I Wayan Supartha; I Wayan Susila; Aunu Rauf; B. Merle Shepard; I Wayan Eka Karya Utama; I Wayan Sandikayasa; I Kadek Wisma Yudha; Putu Angga Wiradana
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.432-444

Abstract

Leaf miners, Liriomyza spp., have developed as a severe pest in recent years, reducing the production of several ornamental and crops in Indonesia. As a result, monitoring and control mechanisms have been proposed. This research aimed to investigate community structure and identify Liriomyza spp., which impacts crops and natural parasitoids in Bali Province. Leaf samples of vegetables, ornamental plants, and related species were collected in all regencies/cities of Bali Province in 2019 and 2020 and preserved in the laboratory for observation and counting of leaf miners and related parasitoids. The findings revealed that four Liriomyza spp. were detected in host plants, namely Liriomyza sativae Blanchard (Diptera: Agromyzidae), Liriomyza huidobrensis Blanchard (Diptera: Agromyzidae), Liriomyza chinensis Kato (Diptera: Agromyzidae), and Liriomyza trifolii Burgess (Diptera: Agromyzidae). Asteraceae species were the most common host plants attacked by leaf miners in the field. The same occurrence was discovered at both heights (high and lowlands). The dry season is the most crucial component in the abundance of Liriomyza spp. In addition, parasitoids of the type Opius cromatomiyae Belokobylskij and Wharton (Hymenoptera: Braconidae) and Hemiptarsenun varicornis Girault (Hymenoptera: Eulophidae) were identified in abundance in Asteraceae plants. Similarly, the parasitization rate of the two parasitoids was found to be high in plants of the Asteraceae and Brassicae families. This approach highlights the decision-making process for controlling Liriomyza spp. on diverse host plant families by developing ecologically beneficial and sustainable parasitoids.
COI Gene Analysis of Asian Horseshoe Crab in Banyuasin Estuarine Waters, Sumatra, Indonesia Fatimah Fatimah; Apon Zaenal Mustopa; Fauziyah; Anna Ida Sunaryo Purwiyanto; Langgeng Priyanto; Nita Puspita Sari; Fitri Agustriani; Rozirwan Rozirwan
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.567-575

Abstract

There is limited study on DNA barcoding of horseshoe crabs in South Sumatra, Indonesia. The present study was the first to record horseshoe crabs' COI DNA barcoding in three localities of Banyasin estuarine waters. The nucleotide composition was in a strong adenine thymine bias (AT = 63.90%) with high haplotypic diversity (Hd = 0.858) relative to low nucleotide diversity (π = 0.0534). A total of 16 haplotypes were recorded, of which two were in Carat Cape, 4 in Makati Jaya, and 12 in the Banyuasin river estuary (BRE), and Hap 1 was a dominant type (62.5%). The population differentiation (FST) value was varied, and only in the comparison of BRE-Carat Cape was significant (FST = 0.637), while the gene flow (Nm) value in Makati Jaya-BRE was high (6.563). The neutrality test, Tajima's D (0.4142), and Fu's Fs values were negative (-0.492), suggesting these populations have experienced a current bottleneck or multiplication. Analysis of molecular variation (AMOVA) suggested that 68% was distributed within populations. The haplotype network, PCoA, and phylogenetic of COI DNA sequences clustered in three groups correspond to Carcinoscorpius rotundicauda, Tachypleus gigas, and Tachypleus tridentatus. This finding is a matter of concern for managing and conserving horseshoe crab species in Banyuasin estuarine waters.
In Silico Analysis of 14-Deoxy 11, 12-Didehydro Andrographolide (AGP 2) from Sambiloto (Andrographis paniculata) as Drug Candidate Against SARS-CoV-2 Muhammad Vicky Astria; Lud Waluyo; Roimil Latifa; Moh. Mirza Nuryadi; Rr. Eko Susetyarini; Mohd. Affendi Labo
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.480-490

Abstract

The outbreak of the COVID-19 pandemic in the world has urged researchers to develop a vaccine or therapeutic drugs to fight this virus. This study aimed to assay 14 deoxy-11,12-didehydroandrographolide (AGP 2) ability as an inhibitor of 3-chymotrypsin like-protease (3CLPro), Papain-like protease (PLPro), and RNA-dependent RNA-polymerase (RdRp), the viral proteins of SARS-CoV-2 and to evaluate it safeness as a drug candidate. In-silico technique was performed in this study to analyze the binding interaction, complex stability between protein and ligand, and drug-likeness properties. The proteins and ligands were obtained from Protein Data Bank (PDB) and PubChem web tools, then using PyRx to identify the binding affinity score, PyMoL to visualize the 3D binding interaction, and WebGro web tools to analyze the stability of each complex. A drug-likeness evaluation was done using SwissADME, pkCSM, and Way2drug web tools. The result of this study showed that the binding affinity score for each complex is; AGP 2-3CLPro (-6.7 kcal/mol), AGP 2- PLPro (-6.4 kcal/mol), and AGP 2-RdRp (-7.0 kcal/mol) where the AGP 2-RdRp and AGP 2-3CLPro showed a stable form indicating the inhibitor ability of AGP 2. This study also demonstrates that the drug-likeness properties of AGP 2 are safe to use. Additionally, it has been proved that AGP 2 can be developed into a therapeutic drug with further studies.
Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines Omat Rachmat Hasbullah; Ilma Fiddiyanti; Dewi Ratih Handayani; Endang Sutedja; Darmadji Ismono; Nucki Nursjamsi Hidajat; Wahyu Widowati; Ervi Afifah; Hanna Sari Widya Kusuma; Rizal Rizal; Firman Alamsyah; Warsito P. Taruno
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.522-531

Abstract

Osteosarcoma is a highly malignant primary tumor on bone that mainly attacks children and young adolescents. Until now, osteosarcoma therapy still combines some high costs and invasive therapy modalities that may give side effects, such as pain and nausea. Our previous studies suggested that non-contact electric field has anti-proliferative effect on breast cancer cells, in vitro and in vivo. In this study, we were interested studying alternating current electric field effects on osteosarcoma cells progression as well as its potential cytotoxic effects. MG-63 human osteosarcoma cells were cultured and treated with 200 kHz for 6 days. Several genes of interest including p53, p21, MDM2, caspase-3, caspase-8, and caspase-9 were analyzed using real-time qPCR method. Apoptotic index was measured using flow-cytometry assay. Apoptosis was observed through p53-independent p21 pathway (p = 0.011). Cells undergoing apoptosis through internal pathways were shown by the increase of caspase-3 (p = 0.015) and caspase-9 (p = 0.001) levels, but not caspase-8 (p = 0.080). This treatment has successfully reduced the number of living osteosarcoma cells by 14.7% (p = 0.000) and increased cell death up to 4.26% (p = 0.055). Apoptotic index was markedly increased to 16% (p = 0.001). 200 kHz non-contact electric field exposure can disrupt osteosarcoma progression through disruption of normal cell cycle via p53-independent p21 pathway and induction of apoptosis.
Ichthyofauna Diversity and Its Distribution in a Low-Saline Lake of Indonesia Ahmad Muhtadi; Fredinan Yulianda; Mennofatria Boer; Majariana Krisanti; Desrita
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.421-431

Abstract

Lake Siombak is a tidal lake that is an estuary around the lake covered with various mangrove trees. It is interesting to study the fish's spatial and temporal distribution as a unique and distinctive tidal lake ecosystem. This research was conducted for a year (from September 2018 to August 2019). Fish sampling was performed by installing gill nets (mesh size 1 inch) and cast nets (mesh size 0.5 inch). Gill nets and cast nets were installed at high and low tides during the full moon. This research showed that there were 27 species of 20 families. Tilapia, Mudskipper, Indo-pacific tarpon, and mainly Rice Fish are always found in Lake Siombak. The fish group at Lake Siombak consists of freshwater fish (mainly: Tilapia, Catfish, and Snakehead), brackish fish (mainly: Mudskipper, Rice fish, and Indo-pacific tarpon), and marine fish (mainly: Milkfish, Kanda mullet, and John's snapper). The actual resident in Lake Siombak consists of Oryziidae, Tilapia, and mudskipper. Other fish are temporary resident fish except for Lutjanidae and Scatophagidae as visitor fish during high tide and high salinity periods (dry season) and Synbranchidae during low salinity (rainy season).

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