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MEDIA PETERNAKAN - Journal of Animal Science and Technology

mediapeternakan
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Published by Institut Pertanian Bogor

ISSN : - EISSN : - DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

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Articles 12 Documents

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Search results for , issue "Vol. 35 No. 3 (2012): Media Peternakan" : 12 Documents clear

Growth Hormone Gene Polymorphism and Its Association with Partial Cumulative Milk Yields of Holstein Friesian Dairy Cattle R Misrianti; A Anggraeni; E Andreas; C Sumantri
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Growth hormone gene (GH gene) plays an important role in regulating body growth and in developing mammary gland, similar with its interaction to specific receptors. The GH gene has been considered as one of candidate gene associated with selection on lactation trait and milk production. This study was aimed to determine genetic polymorphism of the GH-AluI gene and to associate its genotype variants on various 15-d partial cumulative milk yields in Holstein Friesian (HF) dairy cows. A number of 370 blood samples were collected from six HF populations, respectively from small dairy farmer under the supervision of the North Bandung Milk Cooperation (NBMC) in Cilumber (98) and Pasir Kemis village (96), Dairy Cattle Breeding and Improvement Station (Cikole DCBIS) Cikole (88), Lembang Artificial Insemination Center (Lembang AIC) (17), Singosari Artificial Insemination Center (Singosari AIC (32), and Cipelang Livestock Embryo Center (Cipelang LEC) (40). A polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method was used to identify variant genotypes of the GH gene using AluI restriction enzyme. Genotyping results produced only two genotypes, i.e. LL and LV genotypes, without VV genotype. Frequency of the former was dominant, whilst that was low for the latter (89% vs. 11%); leading to the frequency of L allele was very high (94%) compared to that of V allele (6%). No significant association between variant genotypes (LL and LV) and various 15-d partial cumulative milk yields.

Microbial and Oligosaccharides Treatments of Feces and Slurry in Reducing Ammonia of the Poultry Farm Y Yusrizal
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study was conducted to investigate the effectiveness of Lactobacillus sp and fructooligosaccaride (FOS) to reduce the volatile ammonia formation from chicken excreta and layer slurry. For each treatment-replication, 150 g of fecal material were collected from the poultry farm and placed in 500 ml beaker glass. The fecal sample was then treated with 2% Lactobacillus sp (2.6x106) cfu/g and 2% FOS and covered with plastic wraps. The volatile ammonia contents and pH were measured after one hour of standing (0 d) and repeated at 48 h intervals for 6 d. For the dropping slurry study, 300 g of each layer dropping slurry sample were used. Results indicated that 2% Lactobacillus sp or FOS supplementations in the feces and dropping slurry after 1 h up to 6 d reduced the ammonia odor formation, fecal pH, and moisture content. The Lactobacillus sp and FOS treated manure resulted in increasing Lactobacillus sp count and reducing in E. coli, Salmonella, and Campylobacter in 6 days for both feces and layer dropping slurry. In addition, reducing moisture content was observed in treated manure. It is concluded that Lactobacillus sp and FOS reduced the volatile ammonia formation and pathogenic bacteria from chicken excreta and layer slurry.

Allelic Frequency of Kappa-Casein Locus (Asp148/Ala) in F1: Simmental (Bos taurus) x Ongole Grade (Bos indicus) M A Mu'in; A Supriyantono
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study was conducted to detect the genetic variants (single nucleotide polymorphism) of kappa-casein locus (Asp148/Ala) in F1: Simmental (Bos taurus) x Ongole grade (Bos indicus), SIMPO. Genomic DNA was isolated from blood sample of 40 SIMPO (21 males and 19 females). A 780 bp specific fragment of kappa-casein gene spanning from the forth exon region (517 bp) to forth intron (263 bp) was successfully amplified. The result of the PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphisms) analysis using HindIII enzyme showed that two genotypes (AA and AB) were found at this locus in SIMPO. The frequencies of A and B alleles in SIMPO were 0.79 and 0.21, respectively. The frequency lies between B. taurus (Simmental) and B. indicus group.

Passive Immunization of Anti bZP3 (Zone Pellucida3) in Wistar Rat (Rattus novergicus) and Mouse (Mus musculus) Y Pantiwati
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study was aimed at comparing the influence of anti bZP3's passive immunization on anti-anti bZP3's titer and pregnancy level on Wistar rats and mice. This study employed factorial design experiment with completely randomized design. The first factor was immunogenic type. The treated rats were immunized with 100 L anti bZP3 in 100 L Complete Freund's Adjuvant (CFA), while the treated mice were injected with 50 L anti bZP3 in 50 L CFA. Control Wistar rats and mice were immunized with CFA and Incomplete Freund's Adjuvant (IFA) without anti bZP3. The second factor was animal type. The third factor was the length of serum incubation, i.e. 38, 49, 63, 86, 100, and 126 d. Dot blot on the treated Wistar rats and mice showed positive response proven by blue gradation; pre-immune mice as well as control Wistar rats and mice showed negative response proven by white gradation. The highest antibody titer in treated mouse serum was shown in 63 d incubation. The pregnancy on treated mice, control mice and Wistar rat occurred 100% until day 126; while the failure percentage on the treated mice was 4.5%. The pregnancy on treated mice occurred in 86 d incubation (1 rat), 100 d incubation (1 rat), and 126 d incubation (3 rats). Effective passive immunization on similar hospes occurred until day 63; while different hospes was ineffective. Antibodi anti-bZP3 was potential as a contraception through passive immunization on similar hospes.

Assessment of Oxidative Stress in Peste des petits ruminants (Ovine rinderpest) Affected Goats A K Kataria; N Kataria
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of the present investigation was to evaluate oxidative stress in goats affected with peste des petits ruminants (PPR). The experiment was designed to collect blood samples from PPR affected as well as healthy goats during a series of PPR outbreaks which occurred during February to April 2012 in different districts of Rajasthan state (India). Out of total 202 goats of various age groups and of both the sexes, 155 goats were PPR affected and 47 were healthy. Oxidative stress was evaluated by determining various serum biomarkers viz. vitamin A, vitamin C, vitamin E, glutathione, catalase, superoxide dismutase, glutathione reductase and xanthine oxidase, the mean values of which were 1.71±0.09 µmol L-1, 13.02±0.14 µmol L-1, 2.22±0.09 µmol L-1, 3.03±0.07 µmol L-1, 135.12±8.10 kU L-1, 289.13±8.00 kU L-1, 6.11± 0.06 kU L-1 and 98.12±3.12 mU L-1, respectively. Each parameter analysis of variance showed highly significant effect (P=0.0001) of health status and age category. Further interaction between health status and age category was also highly significant (P=0.0001) for each parameter studied. The results indicated that vitamins A, C, E and glutathione levels depressed by 18.95%, 38.67%, 47.64%, and 47.39%, respectively and the serum catalase, superoxide dismutase, glutathione reductase and xanthine oxidase activities increased by 90.79%, 75.11%, 90.34%, and 44.06%, respectively in affected animals as compared to that in healthy ones. On the basis of the altered levels of serum biomarkers of oxidative stress it was concluded that the animals affected with PPR developed oxidative stress.

Evaluation of Antimicrobial Efficacy Against E. coli Isolates of Different Serogroups Obtained from Diarrhoeic Neonates of Calves, Kids, and Lambs R Pachaury; A K Kataria
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Fourteen antimicrobials were evaluated their efficacy against 115 Escherichia coli isolates recovered from faecal samples collected from neonatal diarrhoeic calves (66), kids (25) and lambs (24). Of these isolates 37 were nontypeable, four were of rough type and 74 isolates were typeable for 'O' antigen belonging to 19 different serogroups (O2, O3, O5, O17, O21, O22, O25, O41, O45, O55, O60, O65, O70, O76, O114, O116, O147, O152, and O158). Evaluation of antimicrobial efficacy by disc diffusion method revealed that the most effective antibiotics were ciprofloxacin, sparfloxacin and triple sulpha followed by co-trimoxazole, chloramphenicol, nitrofurantoin, gentamicin, nalidixic acid, polymixin-B and amoxyclav in decreasing order of their efficacy while ampicillin and cephotaxime were found totally ineffective against these isolates. All the isolates showed multiple drug resistance to various antibiotics ranging from two to ten antibiotics. The resistance patterns were independent of serogroups of isolates and the animal species from which isolates were obtained. However, there was a variation in resistance pattern for same antibiotic vis-a vis locations of their isolation.

Fiber Quality of Iranian Carpet-Wool Sheep Breeds H R Ansari-Renani
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This experiment was aimed to identify characteristics and comparative merits of Iranian sheep wool breeds. Fiber characteristics of 75 male and 137 female of 1, 2, 3 and 4 yr of age belonging to Afshari, Zandi, Mehrabani, Lori, and Baluchi sheep breeds were studied. Samples of fiber were taken from the left midside of sheep and analyzed using standard objective measurements for staple length (SL), mean fiber diameter (MFD), coefficient of variation of fiber diameter (FDCV), inner coat fiber ICF, outer coat fiber (OCF), kemp fiber (KF) and yield (Y). A general linear model including sex and age as fixed effects and breed as random effect was used to analyze the data. The mean (and s.e) for SL, MFD, FDCV, ICF, OCF, KF and Y were 110.8±0.1 mm, 36.9±0.5 µm, 50.2%±0.8%, 84.2%±0.6%, 9.8%±0.4%, 5.9%±0.4% and 69.3%±0.7% respectively. MFD of 22% of all wool samples was between 22-30 µm. MFD of a further 36% and 42% of wool samples was between 31-37 µm and coarser than 37 µm. SL of 12% of wool samples was shorter than 100 mm and 38% of samples between 100-120 mm and 50% of samples were longer than 120 mm. There is substantial scope to improve the quality of fiber produced by Iranian sheep breeds through genetic selection.

Distribution of Cows by Days in Milk (DIM) at First AI and Calving to Conception Interval in Dairy Cows M Yusuf; A L Toleng; M F Syafar
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The objective of this study was to show the distribution of cows by days in milk (DIM) at first artificial insemination (AI) and the interval from calving to conception. The study was conducted in 47 commercial dairy herds in Enrekang Regency from May to October 2011. Of 289 animals, 143 of them or 49.5% were dairy Holstein Friesian cows with parities one to seven; mean (±SD) 2.05±1.50. The cows were classified into six groups based on DIM at first AI; within 40 d postpartum, between 41 and 85 d, 86 and 115 d, 116 and 150 d, 151 and 210 d, and 211 days or more. The cows were classified into five groups based on the interval from calving to conception; within 85 d postpartum, 86 and 115 d, 116 and 150 d, 151 and 210 d, and 211 days or more. The results of this study showed that the interval from calving to first AI was 131.6±121.8 d. The percentage of cows inseminated within 85 d after calving was only 56.1%; significantly lower (P < 0.01) than the percentage in the list of fertility management assessment standard. Likewise, cows conceived within 150 d after calving was only 32%. In conclusion, a longer average days in milk (DIM) at first AI in dairy cows was found in the present study, subsequently reduced the possibility of the cows to become pregnant in an optimum time, and reduced the reproductive performance of the herds.

The Ability of Immunoglobulin Yolk Recognized the Antigen in the Tissue of Ascaridia galli . Darmawi; U Balqis; M Hambal; R Tiuria; B P Priosoeryanto; E Handharyani
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Antigen-antibody reaction is an important tool for the analysis of localization of target molecules, including antigenic protein within worm tissues. The purpose of the present research was to demonstrate the ability of immunoglobulin yolk (IgY) anti-excretory/secretory recognized the antigen in the tissue of Ascaridia galli by mean of immunohistochemistry method. The excretory/secretory protein was procured from A. galli and concentrated by mean of vivaspin 30,000 MWCO. IgY was produced by egg yolks of immunized chickens with excretory/secretory, and purified using fast protein liquid chromatography (FPLC) method. A. galli adult worms were cut in transversal and longitudinal section of the center and anterior region. Slides were incubated with both primary IgY for overnight at 4 oC and secondary antibody rabbit anti-chicken IgY HRP-conjugate for one hour at room temperature. The slides were stained with 3-amino, 9-ethylcarbazole (AEC) chromogen, counterstained with Lillie Mayer Haematoxylin, and mounted in glyserin aqueous mount. Antigen-antibody reaction was investigated under a microscope. The result showed that antigen was appeared in the tissues such as cuticle, epicuticle, buccal cavity, and eggs inside the uterine of A. galli. This research concluded that IgY stimulated by the excretory/secretory was able to recognized the antigen scattered in the tissues of A. galli so the IgY could be applied for immunodiagnostic.

Carcass and Non-carcass Components of Priangan and Javanese Fat-tailed Rams Slaughtered at Mature Live Weight M Baihaqi; R Herman
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Twenty-three of indigenous Priangan and Javanese Fat-tailed (JFT) ram breeds were used to evaluate its carcass and non carcass components slaughtered at mature live weight. Five Priangan rams and six JFT rams were slaughtered at 32.5 kg of live weight while six other rams of each breeds were also slaughtered at 40 kg of live weight. Before the rams were slaughtered, they werefattened using pellet ration which was formulated according to NRC for fattening 10 kg of lamb, containing 73.3% TDN and 16% protein in dry matter bases. Feed and water were given ad libitum. Rams were slaughtered and dissected when they reached their assigned slaughter weight (32.5 and 40 kg). The results showed that both breeds had high percentage of carcass (53%-55%) with no significantly different between breeds (P > 0.05) either were slaughtered at 32.5 kg or 40 kg. However, carcass of Priangan rams had significantly more muscle, but less fat than Javanese Fat-tailed rams at mature live weight (P < 0.05). Non-carcass components were not significantly different between breeds (P > 0.05) except for head, tail and testes (P < 0.01) and liver (P < 0.05). The edible and inedible portion of non-carcass rangedfrom 30%-32% and 12%-15% of slaughter weight, respectively.

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