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MEDIA PETERNAKAN - Journal of Animal Science and Technology

mediapeternakan
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Published by Institut Pertanian Bogor

ISSN : - EISSN : - DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Arjuna Subject : -

Articles 522 Documents

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Estimates of Genetic and Phenotypic Trends of Growth Traits in Bali Cattle A Gunawan; R Sari; . Jakaria
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of this study was to estimate genetic and phenotypic trends for growth traits including birth (BW), weaning (WW) and yearling weight (YW) in Bali cattle. The number of cattle used to determine growth traits of BW, WW, and YW were 235, 215, and 178 heads, respectively. Estimation of breeding value, phenotypic and genetic correlation were calculated by Restricted Maximum Likelihood and General Linier Model (GLM) procedures, respectively. Genetic trends analysis was performed using the regression mean breeding values on birth year. Phenotypic and genetic correlation among BW and WW were 0.10 and 0.08 respectively and 0.90 and 0.70 for WW and YW respectively. The phenotypic trends for traits of birth and weaning weight were constant, whereas yearling weight was fluctuating from 2000 to 2008. Likewise, in the case of genetic trends, the birth and weaning weight were constant from 2000 to 2008 except for WW in 2005, whereas the genetic trends for yearling weight showed a fluctuation of wide range. According to the breeding value estimated for all traits, the best was the sire No. 0565, whose breeding value for BW, WW and YW were +0.07, +2.79, and +10.25 kg, respectively higher than the mean value of the population. The genetic trends showed that there have been a significant and positive genetic improvement in all growth traits and indicate that selection would be effective. Genetic correlation between WW and YW was high (0.70) which indicates that the selection on weaning weight might also increase yearling weight in Bali cattle.

The Genetic Diversity of TLR4 MHC-DRB Genes in Dairy Goats Using PCR-RFLP Technique M Petlane; R R Noor; R R.A Maheswari
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This research was aimed at evaluating the genetic polymorphism of TLR4 and MHC-DRB genes in dairy goats [(Saanen, Etawah Grade-Saanen Crossbred (PESA), and Etawah Grade (PE)] using PCR -RFLP. The two genes are involved in immunity where they play a crucial role in pathogens recognition and presentation to T-cells and CD4 cells. PCR was used to amplify genomic DNA for TLR4 (382 bp) and CaLA-DRB (285 bp) genes fragments. Genetic polymorphism was detected by digesting TLR4 amplimer with AluI while DRB amplimers were digested with PstI and TaqI in two separate reactions. The results showed that TLR4|AluI was monomorphic and fixed with allele T in all three breeds while DRB|TaqI and DRB|PstI loci were found polymorphic for all breeds. Heterozygosity expected (He) and PIC were found low at both DRB|TaqI and DRB|PstI loci in PE and Saanen. Χ2 results showed that DRB|PstI in PE and DRB|TaqI in PESA were not in H-W equilibrium and did not display homozygous recessive genotype. The results declared that TLR4|AluI was not a good for marker for diseases resistance whereas DRB|TaqI and DRB|PstI gave hope for resistance based on their PIC.

A Novel Single Nucleotide Polymorphism in Exon 4 of Insulin-Like Growth Factor-1 Associated with Production Traits in Bali Cattle . Maskur; C Arman; C Sumantri; E Gurnadi; . Muladno
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Insulin-like growth factor-1 (IGF-1) is one of the gene candidates that can be used in selection strategy by using DNA markers (marker assisted selection). Gene candidate strategy is a molecular biology techniques to identify quantitative trait loci directly, with the assumption that genetic variation associated to quantitative trait variation. This study was designed to identify any new mutations in exon 4 that can cause the IGF-1 gene polymorphism and then affect the production traits on Bali cattle. Single nucleotide polymorphism (SNP) discovery was conducted by using the direct sequencing technique. Genetic variation of the genes candidate was identified by using PCR-RFLP technique. The results of this study indicate the presence of a new SNP in exon 4 of IGF-1 gene caused by the T/C transition, which can be identified using Rsa1 restriction enzyme. Genotypic polymorphism of IGF-1/Rsa1 has a significant influence on birth weight, weaning weight and average daily gain of Bali cattle. CC genotype had a birth weight rate, weaning weight and average daily gain of: 15.64±1.83; 83.15±9.00, and 0.439±0.07 respectively, higher than the TT and CT genotype. IGF-1/Rsa1 can be used as a genetic marker for selection of birth weight, weaning weight, and daily body weight gain.

The Characteristics of Cytochrome C Oxidase Gene Subunit I in Wild Silkmoth Cricula trifenestrata Helfer and Its Evaluation for Species Marker . Suriana; D D Solihin; R R Noor; A M Thohari
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The study was conducted to assess the characteristics of partial gene of cytochrome C oxidase subunit I (COI) of wild silkmoth Cricula trifenestrata, and to detect the diagnostic sites from these gene for evaluation as species marker. A total of fifteen larvae of C. tifenestrata were collected from Bogor, Purwakarta, and Bantul Regencies. Genomic DNA was extracted from silk gland of individual larvae, then amplified by PCR method and sequenced. DNA sequencing was done to characterize their nucleotide and amino acid contents. The results showed that 595 nucleotides at the 5 'end of COI gene of C. tifenestrata was conserved at the species level, but varies at the family level. Nucleotide dominated by thymine and adenine bases (± 70%). There were 25 diagnostic sites for C. tifenestrata, and four diagnostic sites for genus level. One hundred eigthty nine (189) amino acids were alignment, and only one percent of the genes was varied among species. The 107th amino acid (valine) and 138th(threonine) were diagnostics amino acid for C. tifenestrata. Based on nucleotides and amino acids sequences, the phylogeny showed that C. tifenestrata lied on the same nodes with Antheraea, so the Saturniidae family is monophyletic.

Curing Ingredients, Characteristics, Total Phenolic, and Antioxidant Activity of Commercial Indonesian Dried Meat Product (Dendeng) T Suryati; M Astawan; H N Lioe; T Wresdiyati
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Dendeng is Indonesian dried meat product that used spices rich in antioxidant component as ingredient. In addition, commercial dendeng usually use saltpeter (nitrate/nitrite salt) as curing ingredient to inhibit rancidity and to produce stable red color. The aims of this study were to investigate composition of spices and saltpeter added, characteristic, total phenolic, and antioxidant activity of commercial dendeng. This research was conducted through interview with some dendeng producers to get information about saltpeter adding and spices composition used in their products. Then the interview results were verified by laboratory analysis. The results showed that spices and saltpeter adding from some producers varied. The saltpeter added in curing process produced inconsistent red color on commercial dendeng in this investigation. Total phenolic of dendeng from producers ranged from 42.47 to 102.0 mg GAE/100 g DM for raw dendeng, and 36.51 to 95.49 mg GAE/100 g DM for fried dendeng. Antioxidant capacity against DPPH ranged from 79.35 to 379.40 mg VCE/100 g DM for raw dendeng, and 94.30 to 559.40 mg VCE/100 g DM for fried dendeng. Antioxidant capacity of raw dendeng was influenced by phenolic content about 87.2%, but in fried dendeng was only 59.0%. In conclusion, dendeng has a significant antioxidant activity, even after frying, and saltpeter addition was not effective to maintain stable red color in dendeng products.

Performance of Broiler Chickens Offered Drinking Water Contained Water Extracted Beluntas (Pluchea indica L.) Leaf and Sugar Cane A Sudarman; . Sumiati; R Kaniadewi
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Our previous study showed that the addition of water extracted beluntas leaf into drinking water could be used as an anti-stress and improved performance of broiler chicken. However, the addition was only up to 10% of drinking water. The objective of this experiment was to study whether the addition of sugar and water extracted beluntas leaf in levels higher than 10% of drinking water can give greater benefit on performances and meat cholesterol content of broiler. Two hundred sixteen DOCs CP707 strain were reared for four weeks in high stocking density of 15 birds/m2. The birds were subjected to experimental drinking waters with or without water extracted beluntas leaf (v/v) and sugar which is consisted of T1 (only drinking water), T2 (drinking water + commercial antistress), T3 (drinking water + 10% extract of beluntas leaf ), T4 (drinking water + 20% extract of beluntas leaf), T5 (drinking water + 40% extract of beluntas leaf), T6 (drinking water + 80% extract of beluntas leaf), T7 (drinking water + 40% extract of beluntas leaf + 1.6% sugar), and T8 (drinking water 80% extract of beluntas leaf + 3.1% sugar). The results showed that water extracted beluntas leaf addition higher than 10% significantly reduced (P < 0.05) body weight gain, final body weight, and increased (P < 0.05) feed conversion ratio. It is concluded that the addition of water extracted beluntas leaf more than 10% into drinking water cannot give greater benefit on performances as an antistress of broiler. Sugar addition cannot help the disadvantage due to the bitter taste of higher level of water extracted beluntas leaf addition.

Substitution of Concentrate with Mulberry Leaves in Ongole Grade Cattle Fed Rice Straw Based Diet S Syahrir; K G Wiryawan; A Parakkasi; M Winugroho; A Natsir
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

An experiment was conducted to assess the substitution of concentrate with mulberry (Morus spp.) leaves combined with rice straw offered to beef cattle. The study used 12 male Ongole grade cattle weighing 217+10.53 kg. Rations consisted of rice straw, mulberry leaves and concentrate. Research was carried out according to completely randomized design with 3 treatments and 4 replications for each treatment. The treatments were: P1= 50% rice straw + 50% concentrate; P2= 50% rice straw + 25% concentrate + 25% mulberry; P3= 50% rice straw + 50% mulberry. The results of the study indicated that rumen fermentation in cattlle fed on P2 ration was more effective than those receiving P1 and P3 rations. The fermentation was characterized by a higher production of total VFA and allantoin excretion in the urine. The presence of 1-deoxynojirimycin (DNJ) compound of mulberry leaves in the rumen system in treatment P2 and P3 improved digestibility of fiber fractions of rice straw-based rations. Mulberry leaves in rations also accounted for isoacids in the rumen system, which supported the development of cellulolytic bacteria. In conclusion, mulberry leaves containing concentrate had a potency to substitute concentrate when used together with fiber source feed such as rice straw.

Feeding Diets Containing Different Forms of Duckweed on Productive Performance and Egg Quality of Ducks B Indarsih; M H Tamsil
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The present experiment was undertaken to study the feeding effect of diets containing different forms of duckweed for local ducks on their productive performance and egg quality or egg yolk pigmentation. A total of 90 birds of 24 wk old ducks were randomly divided into 18 experimental units of 2.0 x 1.0 m2 of cages. The experiment was assigned in a completely randomized design (3 treatments with 6 replicates, 5 birds each). There were 3 dietary treatments, namely P1= ducks fed a complete diet containing 20 % of dried duckweed and given in the form of dry-mash; P2= a complete diet in P1 but it was offered in wet form (slurry); and P3= ducks were offered basal diet in the form of dry-mash and fresh duckweed was offered separately ad libitum. Diets were formulated to have similar nutritional contents. Feed consumption, feed conversion ratio (FCR), and egg yolk pigmentation were measured. The result of the study showed that these three parameters were affected by the feeding different forms of duckweed. Feeding diet with fresh duckweed brought about the best pigmentation than did the dry one. Fresh duckweed offers a promise as a potential feedstuff for ducks and has a good implication in reducing feed processing cost.

Physiological Adaptation and Biomass Production of Macroptilium bracteatum Inoculated with AMF in Drought Condition S Sowmen; L Abdullah; P D.M.H Karti; D Sopandie
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The objective of this experiment was to evaluate the effect of drought stress and mycorrhizal inoculation on physiological adaptation and biomass production of Macroptilium bracteatum. This experiment was arranged in completely randomized design with four treatments: M0 (no AM + watered), M1 (AM + watered), M2 (no AM + drought), and M3 (AM + drought) with three replicates. The observed variables were soil water content, leaf water potential, leaf relative water content, leaf proline, leaf water soluble carbohydrate (WSC), root and shoot dry weight. Data were analyzed by ANOVA and differences between treatments were tested by DMRT. Drought treatments (M2 and M3) significantly (P < 0.05) decrease soil water content, leaf water potential, leaf relative water content and increased the leaf proline content. The result in root and shoot dry weight appear that M1 treatment was significantly different (P < 0.05) with treatment M0, M2, and M3. For leaf WSC, M0 and M2 treatments were significantly different (P < 0.05) with treatment M1 and M3. It is concluded that mycorrhiza inoculation was more effective on M. bracteatum, in drought stress. One mechanism of drought resistance of M. bracteatum is the accumulation of osmotic compounds proline. Therefore, proline can be used as an indicator of drought resistance in leguminous plants.

The Application of Planned Behavior Theory to Predict the Consumption of Processed Body-Shaping Milk F S Yulianti; S P Syahlani; F T Haryadi
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The research aims were to identify the influence of attitude, subjective norms and perceived behavioral control on intention to buy, and effect of intention to buy and perceive behavioral control over buying behavior on processed body-shapping milk which added by supplement for body shaping. Research was conducted in Sleman Regency and design used was survey. Sampling was taken by using judgmental sampling method with criteria that were female, aged 18-25 yr old and was categorized as normal to overweight appearance. Respondents used were 100 undergraduate female students. Questionnaire was used as an instrument in order for data collecting to measure research variable that had been tested on validity and reliability. Analyses used were single and multiple linier regression. The results showed that only attitude and perceived behavioral control influenced (P < = 0.05) intention to buy in normal-weight group, while in over-weight group attitude, subjective norm and perceived behavioral control affected (P < = 0.001) attitude. Then, attitude and perceived behavioral control influenced buying behavior significantly both in normal and overweight. Therefore, it can be concluded that prediction capability of planned behavior theory are better when product relevancy to respondents was closed.

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