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MEDIA PETERNAKAN - Journal of Animal Science and Technology

mediapeternakan
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Published by Institut Pertanian Bogor

ISSN : - EISSN : - DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

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Articles 522 Documents

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Performance Enhancement and Immunity Profile of Broiler Treated Feed Additive Containing Lactic Acid Bacteria and Ganoderma lucidum A Sofyan; M Angwar; H Herdian; L Istiqomah; A Febrisiantosa; H Julendra; M H Wibowo; T Untari
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The experiment was conducted to evaluate the influence of feed additive containing lactic acid bacteria (LAB) and Ganoderma lucidum (GL) on body weight gain (BWG), feed efficiency (FE), performance index (PI), antibody titer (AT) against Newcastle disease and histopathology of broilers. Bacteria used were Lactobacillus salivarius and Pediococcus pentosaceus, which were isolated from broiler's intestine. A number of 195 unsexed day old chicks (Cobb strain) were arranged in a completely randomized design and consisted of 5 treatments, each in 3 equal replicates. The treatments were as followed T0: control/without-feed additive, T1: 1% LAB (109 cfu g-1), T2: 1% GL, T3: 1% of LAB 109 cfu g-1 + GL (1:1), T4: commercial antibiotic. Data were analyzed using ANOVA and conti-nued to Duncan's multiple range test. The results showed that T2, T3, T4 treatments significantly improved (P < 0.05) BWG, FE and PI of broilers. Broilers fed T3 had the highest PI, followed by T4, T1, T2 and T0. Broilers fed T3 had the highest AT value followed by T0, T2, T4, and T1. Histopathology profile showed that broiler fed T3 had no lesion on liver and intestine compared to others. The result of this experiment indicated that additive containing 0.25% L. salivarius, 0.25% P. pentosaceus, and 0.5% G. lucidum was able to enhance broiler performance.

Efficacy of Dietary Urea-Impregnated Zeolite in Improving Rumen Fermentation Characteristics of Local Lamb D Kardaya; D Sudrajat; E Dihansih
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

A research on dietary inclusion of urea-impregnated zeolite as slow-release urea (SRU) agent had been conducted to reveal its effect on ruminal fermentation characteristics in local lambs. The research used 24 heads of 7-8 mo old of local male lambs with (20.12±2.1 kg BW) designed upon a randomized block design. Treatments consisted of diets contained no urea, urea, zeolite, and urea-impregnated zeolite. The collected data was analyzed with UNIANOVA and Duncan’s multiple-range test. Results indicated that feeding no urea, zeolite, or urea-impregnated zeolite ration produced lower ruminal ammonia nitrogen than feeding urea ration (P < 0.05). Feeding zeolite ration produced lower ruminal pH than feeding urea ration (P < 0.05). Despite total VFAs were similar across the treatments, feeding urea-impregnated zeolite ration produced lower ruminal acetate, acetate to propionate ratio, or methane production than feeding urea ration (P < 0.05). Feeding urea ration produced the lowest molar proportion of branch-chained VFAs (P < 0.05). Feeding urea ration produced higher plasma urea concentration than feeding no urea ration (2.75 mM vs. 2.16 mM; P < 0.05). In conclusion, zeolite or urea-impregnated zeolite as slow-release ammonia or SRU agent was potential in decreasing ruminal ammonia, pH, acetate to propionate ratio, methane, and maintaining low plasma urea within its physiological range.

Cashmere Quality of Iranian Goat Breeds H. R. Ansari-Renani
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This paper assesses the cashmere quality in different Iranian cashmere goat breeds to determine the scope for improvement of fiber quality. In April 2009 midside cashmere samples were taken from a total of 168 male and female cashmere goats of 1, 2, 3, and 4 yr of age. The goats were randomly chosen from Raeini, Birjandi, and Nadoushan breeds respectively from Kerman, South Khorasan, and Yazd provinces. Cashmere yield (CY) was determined from the percentage of weight of dehaired cashmere to weight of shorn fibre. Cashmere fiber diameter was analyzed using a projection microscope instrument. A general linear model including sex and age as fixed effects and breed as random effect was used to analyze the data and measure the relationships between different cashmere characteristics and fleece attributes. The overall means ± standard deviations were for cashmere yield (CY) 51.4%±1.5%, mean fiber diameter (MFD) 18.7±0.2 µm, coefficient of variation of fiber diameter (CVFD) 19.1%±0.3% and staple length (SL) 42.8±1.6 mm. One year old goats had finer cashmere than older goats. CVFD were higher in males and CY and SL were higher in young animals. Iranian cashmere goat breeds have an excellent SL but are relatively coarse. Given the differences between goats there seems to be substantial scope to improve the commercial value of cashmere.

Histomorphology and Physical Characteristics of Buffalo Meat at Different Sex and Age H Nuraini; . Mahmudah; A Winarto; C Sumantri
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of this experiment was to evaluate histology and physical variables of buffalo meat at different sex and ages. Thirty head of buffaloes were used in this experiment. Muscle fiber diameter, fasciculus diameter, thickness of connective tissue, pH, water holding capacity, Warner-Bratzler shear force and cooking loss were measured in this experiment. Analysis of variance of randomized factorial design was used to analyse the data. The differences were analysed by Tukey test. The result showed that muscle fiber diameter was influenced by ages. The difference muscle fiber diameter among ages indicated that 8-12 mo and 1.5 yr old have smaller diameter compared to three year old (P<0.05), but there was no differences between two, three and four years old. The fasciculus diameter, thickness of connective tissue, tenderness, pH value, water holding capacity, and cooking lost indicated no significant found in all stage of age and sex of samples. Meat qualities of buffaloes were not affected by age (2-4 yr) and sex.

Performance and Meat Quality of Broilers Infected with Escherichia coli and Administered with Bio Additive, Probiotic, and Antibiotic L Istiqomah; S N Hayati; E Damayanti; H Julendra; A A Sakti; T Untari
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

An experiment was conducted to determine the efficacy of bio additive administration (a mixture of Lumbricus rubellus extract, Morinda citrifolia leaves extract and lactic acid bacteria), probiotic, and antibiotic to the performance and meat quality of broiler infected with Avian Pathogenic Escherichia coli (APEC). In this study, 140 Jumbo 747 unsexed one-day old chicks were distributed randomly into 20 units of cages, each filled with 7 broilers. Twenty cages were assigned into 5 treatment groups, each treatment in 4 equal replicates. The treatments were as follows: A= E. coli infection (positive control), B= E. coli infection + bio additive, C= E. coli infection + probiotic, D= E. coli infection + antibiotic, E= No E. coli infection (negative control). A commercial corn-soybean-based broiler diet was formulated as the basal diets. The experimental period was 35 d and at 21st d of age the broilers were infected with E. coli except the E treatment. The result showed that bio additive administration (B) increased the final body weight (1,659.52 g) and body weight gain (1,616.81 g) and resulted in less FCR (1.87) among other treatments. The lowest mortality rate was recorded in B treatment (3.57%) and D treatment (3.57%). Probiotic (C treatment) and antibiotic (D treatment) decreased (P < 0.05) meat pH and tenderness compared to other treatments. Meanwhile bio additive administration did not affect the meat quality (pH, cooking loss, water-holding capacity, tenderness, and fat) compared to positive and negative controls. The lowest meat cholesterol content was observed in B treatment (54.02 mg/100 g). It is concluded that bio additive administration on broiler infected with E. coli increased the broiler performance and decreased the meat cholesterol compared to other treatments.

Identification of Single Nucleotide Polymorphism on Growth Hormone Gene in Aceh Cattle E M Sari; R R Noor; C Sumantri; M Yunus; Han J L; . Muladno
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This research was aimed to identify the changes of nucleotide (Single Nucleotide Polymorphism) growth hormone gene in the population of Aceh cattle. There were 44 samples of DNA sequenced, and a few samples from Gen Bank (M57764). Based on the analysis using MEGA program, it was identified one new mutation on exon five on 2230 bp in which C nucleotide turned into T nucleotide, and this was called Silent Mutation (Leusine-Leusine/ CTC-CTT). The frequency of Single Nucleotide Polymorphism (SNP) genotype on 2230 bp (C/T) was CC (0.36), TT (0.14) and CT (0.50). The genotype TT was not possessed by Aceh cattle from Saree, but possessed by those from Banda Aceh and Indrapuri. Chi-square test showed not significant differences in allele frequencies for three population. The frequency of genotype SNP on 2291 bp (A/C) was AC (0.11) and CC (0.89). The frequency of allele C was higher than allele A and T.

In Vitro Fertility of Post-thawed Epididymal Ram Spermatozoa after Storage at 5 °C before Cryopreservation N W.K Karja; M fahrudin; M A Setiadi
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study addressed the effects of storage duration of epididymides at 5 °C before sperm collection and their fertility after cryopreservation in vitro. Spermatozoa from one of the testes pairs were immediately collected, evaluated and frozen (control group). The remaining epididymides were cooled to 5 °C and stored for 24, 48, 72, and 96 h (experimental groups), after which spermatozoa were collected and frozen as in the control group. Before and after thawing, sperm motility, sperm viability and plasma membrane integrity were assessed. The fertilizing ability of frozen-thawed spermatozoa of each group was evaluated by in vitro fertilization of matured sheep oocytes. Sperm quality (sperm motility, viability, and plasma membrane integrity) at collection and after cryopreservation decreased as the duration of the epididymal storage interval increase (P < 0.05). The motility decreased steadily along the studied time periods. Although, the fertilizing ability of post-thawed epididymal spermatozoa gradually decreased as the storage period was prolonged, the spermatozoa collected from the cauda epididymides stored at 5 °C for up to 96 h were able to fertilize 16%-65% of oocytes in vitro. Results of the present study showed that ram epididymal spermatozoa survive in storage at 5 °C for up to 96 h. These spermatozoa maintain their fertilizing ability and may be suitable for use in IVF and other assisted reproductive procedures.

Application of Electrical Properties to Differentiate Lard from Tallow and Palm Oil . Sucipto; T Djatna; . Irzaman; Tun Tedja I; A M Fauzi
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study aimed to differentiate lard from tallow and palm oil based on its electrical properties, namely conductance, impedance and capacitance. These properties were measured at spectra frequencies of 4.20 to 5.00 MHz in room temperature (26-27 oC). Statistic multivariate that consist on principal component analysis (PCA) and cluster analysis (CA) were used to evaluate the data. The results showed that lard and tallow can be differentiated using whole parameters electrical properties of materials. On the other hand, lard and palm oil can only be differentiated using part of the material electrical properties. Good performance of differentiation process was obtained using PCA model at 4.91 to 4.98 MHz. The first two components of PCA, which was derived from conductance, impedance and capacitance, contributed more than 90% of the total variances. CA showed that lard and tallow are different groups based on the Euclidean distance of each electrical properties. This technique can be potentially developed as an electrical sensor for differentiation lard to tallow and palm oil.

Productivity of Local Goats Supplemented with Acacia villosa and Coripha gebanga A M Fuah; W A Pattie
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Most feed for goats in the villages of West Timor, Indonesia, came from communal grazing areas, consist of native grass during wet season, crop residues and tree leaves (Sesbania and Acacia species) in dry season. This study was aiming at examining the growth of goat supplemented with local feed. Twenty four goats were used, the average initial live weight was 12.5 kg. Four feeding treatments were applied: T1-200 g cut grass; T2-200 g Acacia villosa; T3-200 g Coripha gebanga; T4-100 g A. villosa + 100 g C. gebanga. Live weights and feed consumption were analyzed using repeated measures, analysis of variance. The average live weight showed a small increase, as well as daily weight gain of goats of which different significantly (PA. villosa, C. gebanga, and A. villosa plus C. gebanga, respectively. Goats given C. gebanga and mixed Acacia and C. gebanga gave higher average weight gain, but also consumed more feed than those given cut grass or A.villosa (P < 0.01). Supplementing feed to maintain growth of goats during dry seasons was better on A. villosa than on palm pith and its combination.

The Quality of Stallion Semen in Skim Milk and Dimitropoulos Extenders Preserved at 5 oC and Ambient Temperature Supplemented with Different Sugar R I Arifiantini; B Purwantara; T L Yusuf; D Sajuthi
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study was conducted to evaluate the effects of sugars supplementation in skim milk based (SM) and dimitropoulos (DV) extenders on the sperm motility and viability in stallion semen storage at 5 oC and ambient temperature (24-29 oC). Semen samples were collected from 3 stallions; evaluate individually and each of them divided into 8 aliquots. Four out of eight aliquots were diluted 1:1 with SM, while the remaining four were diluted 1:1 with DV; all were then centrifuged at 1006 g (3000 RPM) for 15 min. The supernatants were discarded, and each pellet was re-diluted with SM (control), SM trehalose (SMT), SM-raffinose (SMR), SM-fructose (SMF), DV (control), DV-trehalose (DVT), DV-raffinose (DVR), and DV-fructose (DVF). The diluted semen were divided into 2 aliquots and stored at 5 oC or ambient temperature. The sperm motility and viability were evaluated every 3 h on chilled semen stored at ambient temperature, and every 12 h on those stored at 5 oC. Results of the experiments demonstrated that sperm motility and viability in DV extender significantly higher (P < 0.05) in both temperature. The supplementation of fructose was the best on the motility and viability of the sperm at both temperatures compare to trehalose, raffinose, or the control group. The best extender and sugar combination was DVF, which the total motile sperm stored at 5 oC for 96 h was 45.1% followed by to DVT (40.2%) and DVR (39.2%). The sperm motility in DV and SMF were 35.3% and 35.6%, respectively; these were higher than those diluted with control (28.9%); SMT (30.3%), and SMR (29.6%). The study concluded that the supplementation of fructose in DV extender (DVF) was the best combination to preserve stallion sperm motility and viability stored at 5 oC or ambient temperature.

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