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Indian Journal of Forensic Medicine & Toxicology
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Articles 5,497 Documents
Ability of Staphylococcus spp. Isolated from Meningitis Patients to Biofilm Formation Abbas Hameed Sh. Al-Wandawy1 , Luma Abdulhady Zwain2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11828

Abstract

A total 248 clinical samples of cerebrospinal fluid (CSF) were collected from meningitis patients, for theperiod of July to October 2018 from the Child Protection Teaching Hospital in the Medical City Compoundin Baghdad. All isolates were identification depending on macroscopic, microscopic, biochemical testsand definite with Vitek-2 compact system. The results showed a growth in 42 samples. Sixteen isolates ofStaphylococcus spp. were obtained from samples. The number (percentage) of isolates according to speciesas follow: 7/16) 44%) of S. epidermidis, 3/16 (19%) of S. hominis, 3/16) 19%) of S. haemolyticus, 2/16(12%) of S. aureus, 1/16 (6%) of S.warneri. These isolates were tested for ability to biofilm formation byusing Luria Broth and Tryptone Soya Broth (TSB) supplemented with 0.25% and 2% glucose. The abilityof the isolates to form biofilm with Luria Broth, for S.epidermidis, S. aureus, S. haemolyticus, S.hominis andS. warneri were 7/7 (100%), 1/2 (50%), 2/3 (66.66%), 1/3 (33.33%),1/1 (100%) respectively not formingbiofilm.As for using a TSB supplemented with 0.25% glucose, S.epidermidis, S. aureus, S. haemolyticusand S.hominis were 6/7 (85.71%), 2/2 (100%), 2/3 (66.66%) and 3/3 (100%) respectively were weaklybiofilm formed and when using TSB supplemented with 2% glucose, showed that all Staphylococcus spp.(100%) were positive for the test..The study showed the effect of minimum inhibition concentration(8 ?g/mL) and minimum bactericidal concentrationand (16?g/mL) of Erythromycin on biofilm of Staphylococcusspp. Staphylococcus spp. isolated from CSF showed weak ability to biofilm formation and depending on thehost content of sugars . This study showed also weak biofilm forming of Staphylococcus spp. was resistenceto erythromosin.
Evaluating the Role of High Molecular Weight Adiponectin in Women with Polycystic Ovary Syndrome Treated with Omega-3 Fatty Acids Fadhil A. Nasser
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11829

Abstract

Polycystic ovary syndrome (PCOS) is a significant health problem affecting women during the reproductiveage and characterized by hormonal disturbance resulting in irregular menstrual periods and infertility.Adiponectin is a protein produced by adipocytes and involved in glucose and lipid metabolism. This studyincluded the evaluation of two doses of omega-3 (300 and 600mg/day for 14 weeks). Age, body massindex (BMI), fasting blood glucose level, homeostatic model assessment (HOMA), lipid profile, luteinizinghormone (LH), follicle-stimulating hormone (FSH), prolactin, estrogen and HMW adiponectin levels wereevaluated. Our data showed no significant differences between control group and treatment groups in BMI,age, sex hormones, fasting blood glucose, and HOMA levels. However, the level of HMW adiponectin wassignificantly higher in women treated with 600mg/day for 14 weeks (p < 0.0001) but not 300mg / day ofOmega-3 fatty acids for the same period.
Levels of Leptin and IL-6 With Some Biochemical Parameters in Iraqi Obese Children Asmehan Adnan AL- naqeeb1 , Salwa Gahzi Turki2 , Zahid Jasim Muhamed2, Wesam Hameed Ahmed3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11832

Abstract

Obesity in children is attended by increased circulating leptin concentrations. Girls have higher leptinConcentrations than boys. IL-6 levels related positively with BMI and percent fat mass (PFM).The Objective: Was to detect the serum IL-6 and levels of leptin among girls and boys as obese and otherbiochemical parameters related with obesity. Methodology: This study involved 60 children were classifiedinto 2 groups; 30 obese ones (fifteen girls and boys fifteen) and 30 control (fifteen girls and fifteen boys)with years of seven- fifteen old who attended to the Obesity Research and Treatment Unit/ Al kindy MedicalCollege, during the period January 2017 – July 2017. A clinical examination and diagnosis were done foreach patient by the consultant medical staff and the biochemical laboratory examination results (FBS, bloodurea, serum creatinine, lipid profile, serum GOT, serum GPT, SCa and uric acid) were as a routine work.They compared with 30 healthy children with approximate age with patients. The sera were assessed forIL-6 and leptin by an ELISA kit. Results: Showed no significant differences among obese children (boysand girls) except the GPT and GOT were both higher significantly (P<0.05) in male (42.46±34.27 and33.3±13.6) than in girls (53.6 ±75.1 and 29.9±14.1). Whereas, obese girls revealed a significant increasingin (STRIG 120.3±26.7 P?0.01, SLDL 89.3±25.5 P?0.05, GOT 33.1±13.6 P?0.01, GPT 29.9±14.1 P?0.01and LIPTIN 2758.4±539.1 P?0.001) in comparison to the control girls. Similarly, for boys highly significantincreasing appeared in (SLDL 90.92±18.1, GOT 42.46±34.27, GPT 53.6±75.1 and LIPTIN 2883.93±512.7P?0.001), as well as in STRIG .69±.13 P?0.05 then normal boys. Inversely, it has been shown that (BURIA26.7±5.3 P?0.05, SCREAT .69±.13 P?0.01 and SVLDL 20.15±6.3 P?0.01) were decreased significantly.In boys IL-6 showed significant positive correlation with BMI, while leptin revealed strong associated waswith IL-6 Conclusion: Obesity associated with elevated blood leptin and IL-6 levels. Leptin and IL-6 playkeys role as a biomarker for childhood obesity, and can help in the prediction of get weight in obese childrenas well as its important interrelationship with other parameters.
Study the Histopathogic Effects of Arsenic Trioxide on Some Parenchymatous Organs of Male Rats and Under Alphalipoic Acid Khalil H. Aljeboori 1 , Khalil Gzar2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11833

Abstract

This study was designed to evaluate the histopathogical effects of arsenic trioxide (ATO) on the some organsof rats , for this reason group of rats (15) were I/P inoculated with 1.5 mg /Kg .B.wt . daily for 30 days othergroup( 15 rat) treated with 100 mg/kg.B.wt of alphalipoic acid and ATO ( 1.5 mg/ kg.B.wt) for 30 .Theresults was revealed that the extensive pathological lesions in the liver , kidney , spleen and testes in all ratsexposed to arscenci trioxide due to the pathotoxic effect of ATO and decreased lesions were in the grouptreated with the alpha lipoic acid .100 mg / kg .B.wt) for 30 days as ameliorative or protective effect againstpathotoxicity of ATO .
Claudin is an Immunohistochemical Changes Associated with Experimental Infection of White Mice with 0111 – of non – 0157 Shiga Toxin Producing Escherichia Coli Khalil H. ALjeboori1 , Mukhald A. Ramadhan2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11834

Abstract

Immunohistochemical stain for the tissue sections prepared from experimental infection of mice with 0111 –of non 0157 of shiga toxin producing Escherichia coli .The immunohistochemical changes revealed that theshiga toxin produced by 0111 –STEC effect on epithelial tight Junction ,The IHC stain was directed to detectthe claudin ( the structural protein of tight Junction ) revealed absence of this protein in the paracellular siteswith discohesion of the epithelial cells in the intestine and endothelial cells in the brain blood vessels whichwas responsible for induction of diarrhea and nervous signs
The Impact of Gold and Silver Nanoparticles on The DNA Yield of Mycobacterium Tuberculosis MTB Zaid Mohammed Joodi Al- Janabi1 , Arshed H.Yaseen2 , Raghad Abdullah Hassan3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11835

Abstract

The objective of this research study whether nanoparticles of gold (Au NP) and nanoparticles silver (Ag NP)have an effect on the yield of DNA from Mycobacterium tuberculosis (MTB) in this experiment when it wasextracted DNA from MTB using gold and silver nanoparticles at different concentrations (l0?l, 50?l and100?l) then showed a significant impact on the yield of DNA, especially gold nanoparticles compared withDNA extracted without any of the nanoparticles.by Using a graph obtained from the REALTIME qPCR,which became very clear when the control sample (MTB without any nanoparticles) showed a high value forthe Ct (cycle threshold), which meant that low quantities of DNA. While samples containing nanoparticleswere low in Ct which meant a high yield of DNA was shown. Ct values also decreased when the concentrationof nanoparticles increased, eventually we conclude from the above experiment that nanoparticles showedhigh yield of MTB DNA. These results are likely due the interaction of the nanoparticles utilized with thecell wall and cell membrane of bacterium, which has facilitated the yield of DNA. Further refinement can beto the above said method and the nanoparticles could possibly use as an effective component of Nucleic acidextraction kits for molecular diagnosis and research so that a superior yield is obtaine.
Molecular Investigation of fliC and blaTEM1 Gene among Clinical Isolates of Salmonella Typhi Hala Ridha Abbas Al-Fahham
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11836

Abstract

This study aimed to isolate and diagnose Salmonella typhi by flic gene and investigated the presence ofblaTEM1 gene by using PCR technique and electrophoresis systems in Najaf Province includes 40 clinicalspecimens blood for both sexes with an age ranged between )5-60) years old .The study result by using a flicgene was detected in 30 of the 40 isolates of S. typhi isolated from typhoid patients , which was conducted inthe diagnosis of bacteria concluded that the technique PCR are the most sensitive in the detection of S. typhicompare with other way, blaTEM-1 was detected in 16 of the 40 isolates from S.typhi . The conseder the bla TEMgene encoding TEM-1 b-lactamase is think to give resistance only to penicillins and early cephalosporins;thus , the spectrum resistance of TEM-1 descendants may extend to second, third and fourth-generationcephalosporins.
Correlation of Toxoplasmosis Seroprevalence and Serum Level of Interleukin-10 in Iraqi Breast Cancer Women Maysoon Abdul-zahra Merdaw1 , Zeina Anwar Jaffar2 , Zahraa Mohammed Ali3 , Heba Hashim Khalil4
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.11837

Abstract

Background: Toxoplasmosis is regarded as one of the most important global life-threatening diseases inimmune-compromised people. The intracellular protozoon Toxoplasma gondii is the causative pathogen oftoxoplasmosis. Aim of this study is to investigate the possible association between T. gondii infection andbreast cancer (BC) in Iraqi women, also to assess the effect of T. gondii on interleukin 10 (IL-10) of theimmune response. By ELISA method, blood samples from 81 women with breast cancer and 60 apparentlyhealthy women have been examined for presence of anti-toxoplasma antibodies, also the levels of serumIL-10 were estimated in these subjects. Results showed that women with BC had the highest prevalencerate of toxoplasmosis. The anti- T.gondii antibodies was 38.27% for IgG and 4.93% for IgM, while inthe control group it was 26.66% and 0.0% respectively, with no significant difference for IgG (P =0.148)and IgM (P = 0.081). A highly significant difference was found in the level of IL-10 between BC womenwith toxoplasmosis (10.24%) and BC women without toxoplasmosis (3.6%). These findings suggest thatincidence rate of T. gondii infection is higher in breast cancer women and higher level of IL-10 than theircross-matched control subjects. Therefore, we recommend the inclusion of a screening test for toxoplasmosisand the level of IL-10 in BC patients as in their routine workup.
CD14 C(-260)T Polymorphism and Blood Levels of the Soluble Endotoxin Receptor CD14, their Association with Risk of Ischemic Stroke in Iraqi Populations Manal M. Kadhim1 , Ali S. Shakir2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11838

Abstract

Background: Stroke is a main cause of death and adult long-term disability worldwide, and stays as anenormous burden for society due to lack of illustrated etiology and effective treatments. Aim: the presentstudy was conducted to investigate the association of CD14 genotype and plasma levels of soluble(s) CD14with risk of ischemic stroke. Methods : A total of 40 patients with ischemic stroke were included in the studywho were admitted to hospital from the period between March to August 2019, and other groups consistof 40 apparently healthy individuals. A five ml of blood samples were collected, 2 ml of each sample forpolymerase chain reaction amplification and detection of CD14 technique. The remaining (3ml) for CD14monocyte count by Flow cytometry technique. Results: CD14 C(-260)T genotype was not significantlyassociated with increased risk of IS (P= 0.367). However, sCD14 plasma levels were higher in subjects withTT genotype compared with those with CT or CC genotype (P= 0.013). The count of CD14 Monocytes,in Ischemic Stroke patients was significantly higher than that of healthy control group. Conclusion: asignificant correlation between CD14 genotype and sCD14 levels were higher level in subjects with TTgenotype compared with those with CT or CC genotype.
The Cytotoxicity Effect for the Crude Extract of (Cyperus Esculentus) Tubers on Human Breast Cancer Cell Line (MCF-7) in Vitro Zainab Abd Amir Mezher1 , Liqaa H. Saqban2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11839

Abstract

The study aimed to identify the aqueous and alcoholic extract of Cyperus esculentus tubers in affectinghuman breast cancer (MCF-7) Michigan Cancer Foundation,preliminary diagnosis chemical groups in theseextracts showed the presence of (Tannins, Terpenoids, Resins, Steroids, Flavonoids, Alkaloids, Glycosidesand Phenols). The effect of cellular toxicity of crude aqueous and alcoholic extract of Cyperus esculentustubers in vitro was tested in human breast cancer cells (MCF-7) through the use of six concentrations(midterm scares) (31.25,62.5,125,250,500,1000 ) ?g /mL and for periods of exposure (24, 48, 72) hours.Using the MTT Test. The study found that there is a toxic effect of the crude aqueous and alcoholic extractsof the grain of Cyperus esculentus tubers in cancer cells (MCF-7) and of all the concentrations used, Thehighest percentage and a significant difference (P ?0.01) for the average of the inhibitory average of cancercell growth (IR) was 68, 73 (%) for the aqueous extract and alcoholic extract, respectively, at the highestconcentration of 1000 ?g / mL and after 72 hours of exposure.