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Indian Journal of Forensic Medicine & Toxicology
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Articles 5,497 Documents
Determination and Correlation of Electrolytes and Trace Elements from Vitreous Humour and Synovial Fluid by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) for Estimation of Post-Mortem Interval (PMI): Experimental Study in an Animal Model Panjai Woharndee1 , Jutapit Prungkramphun2, Sarawut Kumphune3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12130

Abstract

The estimation of post-mortem interval (PMI) procedures seem to subjectivity in assessment and lesssensitivity and specificity. Biochemical determination of trace elements from closed-system samples,such as vitreous humour (VH) and synovial fluid (SF) is an alternative laboratory investigation for PMIestimation. The inductively coupled plasma-mass spectrometry (ICP-MS) provides more rapid, precise andsensitive analysis of elements samples. However, determination of electrolytes and trace elements in VHand SF by ICP-MS, and application for PMI has not been intensively investigated. In this study, VH andSF from twenty adult domesticated pig carcasses were collected at 1, 3, 6, 12, 24, 36, and 48-hour afterdeath. Samples were analysed for total protein concentration, Ca, Cu, Fe, K and, Na using ICP-MS. Theresults showed that the protein concentration in VH was significantly increased in time dependent manner,but not for SF. The level of K and Fe in both VH and SF increased in time dependent manner, with a goodcorrelation between both sample sources. The calcium concentration of both VH and SF increased in timedependent manner, but there was no correlation between both sample sources. Sodium was significantlyreduced at 36-48 h, with a correlation between both sample sources. However, the copper level in VH wasincreased at 36-48 h but reduced in SF. In conclusion, this study showed for the first time that multiple traceelements and electrolytes could be detected, using the ICP-MS, in both of VH and SF simultaneously andadditionally its correlation.
Comparison of Surgically Induced Corneal Astigmatism in Cataract Patients after Phacoemulsification Versus Manual Small-Incision Cataract Surgery Giyathaldeen T. Neameh1, Mohammed Qasim Al Nuwaini1, Hayder M. M. Al-Talqani2, Mohammed Qasim Al Nuw
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12131

Abstract

The advances in cataract surgery are aimed at finding a surgical method capable of providing accurate andpredictable results in terms of outcome. Surgically Induced Astigmatism (SIA) is one of the causes of the poorquality of vision post-operatively because of the blurring of images. SIA in turn, depends on the type, lengthand the position of the incision.so this study to compare the mean keratometeric reading preoperative andone month postoperative in phacoemulsification versus manual small-incision cataract surgery and to findwhich produce less surgically induced astigmatism. This was a prospective, observational, cohort study onpatient with cataract carried out from April 2018 to September 2018. This study conducted on (206) patientsattending to ophthalmic department in Najaf. The cases were divided into two groups. Each group had 103patients. One group had undergone phacoemulsification and the other group had undergone superior scleraltunnel incision and follow up after one month to find which procedure less surgically induced astigmatism.Preoperative and postoperative keratometeric was performed after one month. According to this study meankeratometeric reading in the Phaco group preoperative k1= 43. 80, k 2= 44.69 and postoperative k1 =43.71and K2 =44.62.While Mean keratometeric reading in the SSTI group preoperative k1=44.00, K2 =45.02 and postoperative k1 =43.91 and K2= 45.41.Mean astigmatism at one month postoperative in patient undergoing Phaco was 1.3 ±0.9 and undergoingSSTI was 1.3± 0.7, there was no statistical difference in astigmatism at one month postoperative (P=0.5).This study reveals that superior approach in PHACO and MSICS produced similar astigmatic outcome,there is no significant difference in the mean surgically induced astigmatism in both types of surgery
The Improvements in the Prolactin, Thyroid Hormones and Lipid Profiles that are Observed During Three Pregnancy Periods Khalid .S. Sahab1 , Ali. S. Alsaadi2 , Ammar.M.Kadhim3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12132

Abstract

This research was to explore improvements in lipid profile, TSH, T3, T4 and prolactin during threestage of the progress. Pregnancy leads to development in both hormones and biochemistries of thematernal blood. The study involved a control group of 20 non-pregnant women and 30 pregnant and.Automatic hormone analyzer1 and biochemistry automatic analyzer measured hormones, with thelipid profile2. Although T3, T4, TC and TG showed no significant increases in first (p? 0.05) comparedwith the control group, TC and TG increased significantly in the second phase and third stage of pregnancytrimesters and T3 and T4 significantly in the third phase of pregnancy quarter (p ?0.05) compared with thecontrol team (p ?0.05). The results demonstrate a significant increase in prolactin in three stage of pregnancy(p?0.05). No significant increase (p ? 0.05) in comparison with control groups was shown in LDL-C andHDL -C over all phases of the prolactin. Increased prolactin, thyroid hormones and the rise in lipid fractionsfollow normal pregnancy.
Correlation between Seasons and the Prevalence of Preeclampsia at Tertiary Hospital, Indonesia Monika Lijuwardi1, Budi Prasetyo1 , Muhammad Ardian Cahya Laksana1 , Rizki Pranadyan1 , Hanifa Erlin
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12133

Abstract

Background: Preeclampsia is the second most common cause of maternal mortality following postpartumhemorrhage. In East Java alone the maternal mortality rate due to preeclampsia was 31%. Some studiesexplain that the incidence of preeclampsia can be caused by seasonal variations, whereas other studies saythat seasonal factors can be a protective factor for preeclampsia.Objective: To find out how seasons relate to the prevalence of preeclampsia.Method: A descriptive analytic study using medical record data during. The sample meets the inclusioncriteria, namely pregnant women with preeclampsia, severe preeclampsia, chronic hypertension withsuperimposed severe preeclampsia, or eclampsia. The exclusion criteria were pregnant women with chronichypertension or gestational hypertension. The sampling technique used was total sampling and crosssectional design with observation method using secondary data from medical records. Data was processedusing IBM SPSS Statistics and presented narratively.Results: Respondents who experienced preeclampsia in the dry season were 558 people or 19.3% and thosewho did not experience preeclampsia were 844 people or 29.2% The results of data analysis with statisticaltests using Chi-Square obtained a significance value or p-value of 0.091 (p> 0.05).Conclusion: No correlation between seasonality and the prevalence of preeclampsia.
Construction of a New Scale for Biological Estimation of Public Speaking Stress Juliet Maarij Al-Assadi1 , Ali A. Al-fahham2 , Murtadha kanim Adea Aljebory3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12134

Abstract

Stress is a psychophysiological phenomenon that is needed to be quantified from biological aspect forfurther studies and clinical applications .The objectives of the current study was to construct a valid andreliable scale for providing a quantitative estimate of the public speaking stress among academic staff .Amethodological design was applied to achieve the objectives of the study.The study was conducted at theUniversity of Kufa in the city of Najaf. A purposive sample of 200 academic staff was used the presentstudy. A scale of 17 items for public speaking stress have been generated . Content validity was quantifiedthroughout the measurement of the following values : item content validity index (I-CVI) and scale contentvalidity index average (S-CVI/Ave) and content validity ratio (CVR). Two forms of reliability have beenused in the current study : stability (test-retest reliability) and internal consistency . The result of the studyrevealed that the majority of items have been accepted (I-CVI ? 0.8) and the average of all items is accepted(S-CVI/Ave = 0.88). All items have shown acceptable level of reliability between test and retest of thesample (r=0.99). Test of internal consistency (Cronbach’s Alpha reliability) for the overall public speakingstress scale (PSSS) is acceptable (> 0.7). It was concluded that the suggested public speaking stress scale canbe accepted as a valid and reliable scale for quantification of public speaking stress phenomenon.
Larvicidal Activity of Aedes Aegypti from a Simple Preparation of Cashew (Anacardium occidentale L.) Nut Shell Extract for Community Level Use Kowit Suwannahong1 , Kanittada Thongkao2 , Pimporn Thongmuang3 , Torpong Kreetachat4 , Yuttana Sudja
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12135

Abstract

Dengue fever is a major risk to public health due to the recent worldwide spread. Chemical vector control areusually considered, therefore, negative consequences are commonly occurred due to chemical resistance andenvironmental effects. Volume of cashew nut shell is become increasing as by-product after nut processing.This study was aimed to evaluate larvicial activity and toxicity of cashew shell extract with simple preparationfor community use. Fresh cashew nut shell was dried and grinded in powder form. Material was immersed inwater tank for 72 h and ratio of flavor and material and water ratio was 100 g/L (10% W/V). The supernatantof stock solution was diluted to 1:5, 1:10, 1:20, 1:50 and 1:100 of larvicidal solution for phenolic contentmeasurement, analysis of alkyl phenols by gas chromatography-mass spectrometry (GC/MS) and larvicialactivity test. Larvicidal activity was effective at LC50 = 3.8 mg of GAE/ml; LC90 = 11.5 mg of gallic acidequivalent (GAE) /ml. The solution was contained five alkyl phenols, which included saturated cardol,monounsaturated cardol, anacardic acid, diunsaturated cardanol and monounsaturated cardanol. Anacardicacid was major active compound, which was correlated to mass spectra. This larvicidal solution preparationwas simple and cost effectiveness, which may useful in community level especially in cashew cultivatingarea. Field application and public training on this study will be conduct on controlling of mosquito-bornediseases.
Analysis of the Mean Gray Scale and the Mandibular Thickness (Micro-CT) To Mandibular bone Density in Synthetic SCAFFOLD-hADMSC Latief Mooduto1 , Arief Rahman2 , Fedik A Rantam3 , Jenny Sunariani4 , Hendy Jaya Kurniawan5
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12136

Abstract

Introduction. The concept of biofactor transplantation in porous and degradable materials as scaffolds is aplace for regenerating new cell and bone tissue growth in the application of tissue engineering. Human AdiposeDerived Mesenchymal Stem Cells (hADMSC) is a multipotent cell which has osteogenic, chondrogenic, andadipogenic potential. It also has somany similar characteristics with Bone Marrow stem cells (BMSCs) buthADMSC has higher proliferation rate compared to BMSCs.Inspite of this, another ability of biofactors isgene engineering. Aim. This study aimed to analyze mandibular bone density from the synthesis of scaffoldhADMSC with the mean gray scale and the mandibular bone thickness (Micro-CT).Material and Methods.This research involved several processes, namely synthetic scaffold manufacturing process, differentiationand characterization of hADMSC, and Micro-CT. Results. The results of the marked expression of thecharacterization of hADMSCand analysis of the mean gray scale and mandibular thickness (Micro-CT) tomandibular bone density in synthetic scaffold-hADMSC showed to be significant. Conclusions. Syntheticscaffold-hADMSC can increase mandibular bone density after Micro-CT analysis.
The Effect of Trivalent Influenza Vaccines on Immunoglobulin G and Interleukin 4 in Ferret (Mustela putorius furo) Lestari Dewi1,2,, Erman Tritama3 , Reviany Vibrianita Nidom4,5, Kuncoro Puguh Santoso6 , Sri Agus Su
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12137

Abstract

The seasonal influenza virus has infected 5-15% of the human population every year, resulting in 250,000-500,000 deaths worldwide. The seasonal influenza epidemic that occurs every year is caused by thecontinuous evolution of seasonal influenza viruses, which allows them to avoid body immunity due toprevious infection or vaccination, and the ability of these viruses that can be transmitted efficiently fromhuman to human through breathing, direct contact, and through items that have been touched by sufferersof seasonal influenza. Vaccination is the most effective method for controlling the infection of seasonalinfluenza and the most important strategy for pandemic prevention and control. The ideal vaccine mustimprove the humoral and cellular immune response to reduce morbidity and mortality. This study wasconducted to determine the role of trivalent influenza vaccination against the response of ferret antibodiesby testing IgG levels and IL-4 levels. IgG is an antibody formed in response to vaccination, whereas IL-4is a cytokine that stimulates B cells to differentiate into plasma cells and produce antibodies. The methodof this study is an experimental laboratory. Twenty five ferrets separated into 5 group, control, trivalentvaccine dose 3.8 µg, dose 7.5 µg, dose 15 µg, and dose 30 µg. Ferrets were vaccinated with trivalentvaccine, intramuscular, 3 weeks later, that were challenge by H1N1, H3N2 and by influenza H1, H3 dan B(wild subtype) virus. On day 35th serum was taken and examined for IgG and IL-4. The level of IgG andIL-4 was measured by Elisa. We used SPSS 23 for data analysis. From the results of the research conductedenhancement in specific levels of IgG was obtained against H1 and H3 antigen at dose of 3.8 ?g and 7.5 ?g.There is no change in specific levels of IgG against By antigen. There is no change in IL-4 levels. Therefore,we conclude that IgG and IL-4 values can be used as biomarkers in testing influenza vaccines.
The Possible Nephroprotective Effect of Roflumilast Ameliorating Glycerol -Induced Myoglobinuric Acute Renal Injury in Rabbits Model Mukhallad A. Ramadhan1 ; Haider F. Al-Saedi2 ;Ghufran L. Ismaeel2 ; Uday Abdul-Reda Hussein3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12138

Abstract

urther leads to tissue necrosis and inflammation. While, roflumilast N-oxide is an active metabolite ofroflumilast that rising the cyclic adenosine-3?, 5?-monophosphate by inhibiting PDE4.Thirty rabbits were divided into three groups, control group was received normal saline intra-muscularly anddrived from water for eighteen hours before. Second group was received a single dose glycerol injected intramuscularly after drived from water for eighteen hours .Third group was received roflumilast intraperitonealdaily for three days after received a single dose of glycerol .All animals were sacrificed after 72 hour, and then blood collected by cardiac puncture for evaluation thechanges in the levels of renal function tests and histopathological study of rabbit’s kidneys tissues areevaluated evaluation and scoring the changes of tested agents. The numerical data are expressed as means ±standard error means and analyzed by using SPSS version 20.The results of the levels serum urea and creatinine found to be significantly reduced by roflumilast usingANOVA test. Besides, the histopathological changes showed ameliorative effects of roflumilast in comparedto glycerol group. From this study, one could be concluded the protective effect of roflumilast in case ofrenal failure induction by glycerol.
Diagnosis of Empathic Abilities of Medical Students Philipp D. Rasskazov1 , Marina A. Stavruk2 , Lubov A. Kushnyr3 , Anna V. Pichueva3 , Tatiana S. Tsar
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12139

Abstract

The authors of the article focus on empathy as one of the dispositions related to the emotional functioningin the process of communication, which plays an important role in determining success in a profession. Itis believed that people with higher empathy tend to solve professional, personal problems more readily insubject-subject relationships. The technique for diagnosing the empathic abilities of medical students wasbased on a questionnaire developed by V.V. Boyko. Methods of analytical and synthetic study of scientific andmethodological literature, questioning are applied. For empirical data percent distribution and ranking areused. As a result of the study, the following levels of empathic abilities in medical students were evaluated:rational, intuitive, attitudes, penetration, identification. The authors of the article came to the conclusionthat the empathic abilities of medical students are important and influential factors of communication notonly in the doctor-patient model, but also in their interpersonal communication, academic performance ineducational activities.