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INDONESIA
Indian Journal of Forensic Medicine & Toxicology
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Articles 5,497 Documents
Synthesis and Identification and Biological Studies of New Azo Dyes Derived from Imidazole and their Chelate Complexes Fatima J. Ali; Layla A. M. AL-Ameri; AbidAllah M. Ali
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14496

Abstract

Anew azo ligand (E)-2-((4- fluoro phenyl)diazenyl)-4,5-bis(4-methoxy phenyl)-1H-imidazolehas beensynthesized from coupling 4,5-bis(4-methoxyphenyl)imidazole with para-chloro-anline. The structures ofthe new ligand and their transition metal complexes ware investigated and characterized using differentphysicochemical studies as elemental analysis, IR spectra, 1HNMR,, UV-Vis spectra, mass spectra, Molarconductance ,and magnetic susceptibility .The complexes were found to have the general formula [ML2Cl2].H2O where M = Co(II), Ni(II), Cu(II) ,Mn(II),and Fe(II), [MLCl2].H2Owhere M=Zn(II),Cd(II),Hg(II), andPd(II). The IR results demonstrate that the co-ordination sites are the azomethine nitrogen of the imidazolering and azo nitrogen atom of the azo ligand. The electronic spectral and magnetic measurement dataindicate that the complexes exhibit octahedral geometry,except the Zn(II),Cd(II),andHg(II) complexessuggest tetrahedral and Pd(II) a square planar geometry.The cytotoxicity of Pd(II) complex on human LungCancer (SCLC) and normal cells.
The Effects of Mouth Bacterial Infection on Saliva Flow Mohammed F. Jubaz; Rawaa M. Mohammed; Syoof Khoman Alwan
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14497

Abstract

Background: Mouth bacterial infections most probably due to dental cares are a prevalent disease. Salivaryflow rate is affected by many diseases but there is not enough published data about the effect of mouthbacterial infection on the salivary flow rate. Objective: this study examines the relationship between salivaryflow rate and mouth bacterial infection in adult population. Methodology: cross sectional study. Result:salivary flow rate is affected by mouth bacterial infection as it is founded to be very low flow rate in 17% andlow flow rate in 40% of the sample population. Conclusion Salivary flow rate is affected by oral bacterialinfections that need urgent medical intervention to improve the outcome of those patients.
Histogenesis of Skin in Fetal Period of Rabbits Hajar Kamel Ahmed; Mahdi Salih Shallal Alheety
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14498

Abstract

This study was conducted on skin samples from New Zealand white rabbits during the period E15 daysprenatal to E25. to investigate the histomorphological development of the skin, hair follicle, Sebaceousglands.Early in the fetal life (E15) the epidermis is in the form of simple squamous epithelium or stratified Nonkeratinized squamous epithelium of two or more layers, depending on the region studied, while the dermisis in the form of loose connective tissue composed of mesenchymal cells in a loose and irregular shape.(E20) prenatal, the epidermis is stratified squamous epithelium non-keratinized composed of more thanthree layers including the periderm, the intermediate layer and the basal layer in all regions, except Fingeredhand and foot that appearance keratinized squamous. the cells of the mesoderm begin to condense underthe basal layer, indicating the beginning of the formation of hairs placodes. Stratification became evidentin(E25) of prenatal with a marked keratinized and extension of hair follicles in the dermis, accompanied bythe beginning of the development of sebaceous glands in the form of a group of cells. During our study ofthe development of the skin of the New Zealand rabbit before birth, we could not notice any sweat glandsin the examined skin samples.
Molecular detection of Escherichia coli Cause Urinary Tract Infections among Pregnant Women at Thi-Qar province, Iraq Doaa Sh. Al-Hashmay; Shrouk A. Hassan Alibraheem; Khwam R Hussein
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14500

Abstract

The prevalence of antibiotic resistance in Enterobacteriaceae has increased sharply in recent years. Extendedspectrum ß-lactamase-producing Enterobacteriaceae include Escherichia coli has become especiallycommon. Although traditionally linked to risk factors such as prior hospitalization and antibiotic use, thesebacteria have become increasingly recognized in the community, especially as pathogens in urinary tractinfections (UTIs). In this study urine samples from 150pregnant women clinically diagnosed with UTIswere used for Gram staining, culture, API 20 E and singleplex PCR. Singleplex PCR was performed withprimers targeted to chuA and yjaA genes and anonymous DNA fragment TspE4C2 of E. coli. The positivesingleplex PCR products were identified by presence of 279 bp, 211 bp and 152 bp amplicons of chuA andyjaA genes and anonymous DNA fragment TspE4C2 for of E.coli. Conventional methods of Gram staining,culture and API 20E test showed positive result for E. coli in 35 (40%) out of 150 pregnant clinicallydiagnosed with urinary tract infection. PCR detected 24 (27.5%) out of the 35 (40%) samples that werepositive for E. coli. The majority of UTIs caused by spectrum ß-lactamase-producing Enterobacteriaceaeinclude E. coli was acquired in the community, so rapid, specific and sensitive molecular are urgently neededto better prevalence, prevent and treat these infections in Iraq
Molecular Identification of T. mentagrophytes and M. canis Isolated from Children Infected with Tinea Capitis in Basra Governorate by Using AP-PCR Technique Shrouk A. AL-Ibraheem; Nasir A. AL-Mansour; Kadhim k.AL-Rubaiee
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14503

Abstract

The current study included molecular identification of dermatophytes causing Tinea capitis in children inBasrah governorate, including T. Mentagrophytes&M. canis, using AP-PCR or RAPD –PCR polymerasechain reaction technique. By using EZ-10 Spin Column Fungal Genomic DNA Mini –Preps Kit used toextract DNA from dermatophytes fungi, where DNA was obtained from these two dermatophytes fungi.The DNA bands appeared clearly after the electrophoresis of the DNA extract showed the results of DNAamplification .The efficiency of the primeres used in the diagnosis of these two types of dermatophytes, called: randomprimeres OPAA11, OPAA17, OPU15, OPD18, was obtained by the positive results obtained when amplifiedusing these primeres.Used for both dermatophytes The results obtained by using OPAA17 showed successin DNA amplification - obtained from M.canis and T. Mentagrophytes, with six bands for M.canis. As forT. Mentagrophytes, the number of bands obtained was 13, and the amplification results obtained usingOPAA11 showed the efficiency of this primer.Five bands were obtained when used to amplify the DNA of the dermatophytes. While seven bands belongedto T. Mentagrophytes, The amplification results of OPU15 indicated that there were six bands for M.canis,while only one bande for T. Mentagrophytes showed the results of amplification using OPD18 used indiagnosis of dermatophytes to four bands for M.canis while the number of bands for T. Mentagrophytes hasten bands. This method for identifecation is fast and has a high diagnostic efficiency of dermatophytes, asthe diagnosis based on genetic characteristics is more accurate than resulting on phenotypic characteristics .
Isolation of Bacteria and Detection of Cephalosporin Antibiotics Effects among Patients with UTI in al-hilla City Rawaa M. Mohammed; Asma’a HM
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14505

Abstract

90 urine samples were collected randomly from patients whose visit the Urology department in MurjanMedical City in Al-Hilla in period between April to July 2020 , the patients included 35 males and 55females and in age between 17-77 years old. The urine samples divided into two parts , the first part using forgeneral urine examination and the second part using for culture and sensitivity . The results of current studyappeared to 35 (39%) males among total patients infected with UTI while 55(61%) females among totalpatients infected with UTI . In field of effects of age on infection the current results appeared in females theinfection of urinary tract increase in young age group (17-27 years) in percentage (33%) in comparison withother age groups (20 %) in (27-37 years) , (16%) in (37-47 years) , (14%) in ( 47-57 years) , (10%) in (57-67years) and (7%) in (67-77 years) and in males the results appeared increase the UTI with age , (67-77 years)in percentage (32%) in comparison with other age groups, (3%) in (17-27 years) , (9%) in (27-37 years), (11%) in (37-47 years) , (20%) in ( 47-57 years) , (25%) in (57-67 years) . the results of API20 test thatusing for identification of types of bacteria which cause UTI appeared to 34 isolates of E.coli , 15 isolates ofEnterobacter spp. , 18 isolates of Klebsiella spp. , 11 isolates of Pseudomonas spp. , 13 isolates of S.aureusand 4 isolates of Acinetobacter spp. bacteria .In field of antibiotic sensitivity the results appeared the effectsof third and fourth generations of cephalosporin more than other generations .
Iraqi Dentist Response to the Covid-19 Spreading Marwah M. Adnan; Bassam Alsheekhly; Rehab Adil
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14520

Abstract

Introduction: These new generations of virus “COVID -19” now are not the chines nightmare only howeversoon as speedy as feasible spread all over the world and grew to be the most existence threating healthdisorder in the world.Aim: This descriptive research aim to measure the commitment of Iraqi dentists to the Iraqi dental associationrecommendations at the time of lockdowns during the corona virus infection.Result: In this study the Iraqi dentists who had participated in it were 169 from all over the country, thisdescriptive research shows great response of the Iraqi dentists to control infection and reduce the spread of“COVID-19”.Discussion: “COVID -19” is a pandemic disease lead to large changes all over the world, actually dentistrywere one of the careers affected by this disease, the dentist during the daily work with dental slow speedhandpiece or high speed turbine produce droplets and aerosols which probably are contaminated with thevirus “COVID -19”.Conclusion: This study had been showed that the Iraqi dentists have a great commitment toward the societyduring the period of curfew they had been attend to close their privets clinic in order to reduce the viralspread and control the infection.
Bioremediation of Chlorpyrifos Insecticide by using Aeromonas Hydrophila Bacteria Sufyan Mohammed Shartooh; Mohammed Fadhil Abood; Rasha Kifah Hassan
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14523

Abstract

Several bacteria species may have the ability to degrade and digest soundly the residuals of certain insecticidewith causing any deteriorated consequences. This work was designed to examine the ability of Aeromonashydrophila bacteria for biodegrading of chlorpyrifos insecticide from contaminated soil. However, soilsample was collected from home garden at depth of 10 cm while the chlorpyrifos insecticide was obtainedfrom the local market. Meanwhile Aeromonas hydrophila bacteria were isolated from the garden soil. Thebacterial cultural media were prepared to consist of (NH4)2SO4 (1g), NaCl (6g ), K2HPO4 (0.5g), yeastextract (1g ), MgSO4 (0.1g) and CMC (5g). This work has examined the efficiency of A. hydrophila bacteriato remove insecticide from the soil under various growing conditions such as five different pH values,five termperature degrees and four insecticide concentrations. The obtained results showed that the highestbacterial efficiency (98.0 ± 7.8%) was recorded as pH value of 6 and it was 91.1 ± 6.0% at temperature of40 ° C while it was 99.1 ± 6.8 % at insecticide concentration of 25 ppm.
Molecular Co-Localization of Human Epstein Barr Virus Infection Associated with the Expressed Protein of Cyclin D1 \Cyclin Dependent Kinase 4 in Hodgkin’s Lymphoma Tissues Nouri Jaffar Alalsaidissa; Zahraa Ali Abdullah; Ausama Abed-AlkadumAlajeely; Saad Hasan Mohammed Ali; Shakir H Mohammed Al-Alwany
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14524

Abstract

This retrospective case-control study to assess the impact of Cyclin D1 and CDK4 expression along withEBV infection with Hodgkin’s lymphoma (HL).80 lymph node included in this study; (40) formalin-fixed,paraffin- embedded biopsies from Hodgkin’s lymphoma (HL), and (40) apparently healthy lymph nodes ascontrol group. EBV detection was done by CISH whereas IHC detection system was used to evaluate bothCyclin D1 & CDK4 gene expression.The positive –EBERs CISH reactions detected in 16 / 40 (40 %) of Hodgkin lymphoma tissues while positive- EBERs reactions detected in 3 out of 40 tissues (7.5%) in control lymph nodes tissues. Statistically wasfound highly significant between study groups (P value = < 0.05). Cyclin D1 & CDK4 -IHC were detectedin 40% and 52.5% of HL, respectively. Statistically, significant differences noticed among negative, low andmoderate scoring of tissues (P<0.05).Significant EBV gene expressions along with higher percentage of tissues in which Cyclin D1 & CDK4genes being expressed may be indicator their possible role in the pathogenesis and / or the carcinogenesis ofa subset of our Hodgkin’s lymphoma cases.
Histological Features of the Vomeronasal Organ in New Born Lambs (Ovis aries) Dhyaa, Ab. Abood; Safaa Madi Hohammed
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14525

Abstract

Background: vomernasal organ is an auxiliary olfactory organ which located at the base of nasal septumcomposed of olfactory epithelium and being enclosed by cartilaginous capsule. The current study was aimedto investigate the histological structure of vomernasal organ in new born lamb.Methods: six heads of new born lamb were used for this study. The nasal cavity including the hard palatewas sward up into four transvers sections. The sections were processed with paraffin embedding techniqueand tissue sections were stained with H&E stain.Conclusions: The rostral and middle portions of vomeronasals duct had medial and lateral walls with crescentshape lumen and enclosed completely by hyaline cartilage. The medial wall was lined with still diffentiatingneuro-epithelium, possessed many of intra epithelial capillaries and composed of supporting, bipolar neuron& basal cells. The lateral wall was lined with differentiating respiratory epithelium. The lamina propriasubmucosa beneath both neuro and respiratory epithelia composed of ruminant of embryonic mesenchymaltissue contained newly formed glands, blood vessels and nerves. A significant (p<0.05) difference wasrecoded between the diameters of the rostral, middle portions and caudal portion of both gender and therewere no differences between genders. The epithelial height of the neuro epithelia of the middle portion inboth gender had significant value (p<0.05) in compare with epithelia of other portions.