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KLOROFIL: Jurnal Ilmu Biologi dan Terapan
Published by Universitas Indraprasta PGRI
ISSN : -     EISSN : 25986015     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology
KLOROFIL: Jurnal Ilmu Biologi dan Terapan is a journal that published by Major of biology and applied sciences, Departmen Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sumatera Utara Medan, Indonesia.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi
Articles 124 Documents
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PENGARUH PEMBERIAN LARUTAN HASIL FERMENTASI BERBASIS CAMPURAN BAHAN ORGANIK TERHADAP PERTUMBUHAN SELADA MERAH (Lactuca sativa L.) VARIETAS RED RAPID Itsna Safira Ridwan
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 2 (2022): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i2.12822

Abstract

The purpose of this research is to get concentration of fermented solution based on a mixture of organic material which gave the highest effect on the growth of red lettuce (Lactuca sativa L.) Red Rapid varieties. The research method used is an experimental method with a single factor Randomized Block Design (RBD) with 8 treatments and 4 replications, the treatments: A0 (concentration of fermented solution 0 ml/l), A1 (concentration of fermented solution 100 ml/l), A2 (concentration of fermented solution 200 ml/l), A3 (concentration of fermented solution 300 ml/l), A4 (concentration of fermented solution 400 ml/l), A5 (concentration of fermented solution 500 ml/l), A6 (concentration of POC NASA 6 ml/l), and A7 (dose of NPK fertilizer 250 kg/ha). The results showed that there was a significant effect of giving fermented solution based on a mixture of organic material on the average plant height of 7 dap, 14 dap, 21 dap, 28 dap, and 35 dap and the average number of leaves 35 dap. The treatment of A7 (dose of NPK fertilizer 250 kg/ha) obtained the highest results to the average plant height of 7 dap, 14 dap, 21 dap, 28 dap, and 35 dap, meanwhile the treatment of A2 (concentration of fermented solution 300 ml/l) obtained the highest results to the average number of leaves.
PENAPISAN FITOKIMIA DAN PENENTUAN KADAR FLAVONOID EKSTRAK DAUN SEMBUNG [BLUMEA BALSAMIFERA (L.) DC] DI DESA HASANG DAN DESA SIMANGALAM KECAMATAN KUALUH SELATAN KABUPATEN LABUHANBATU UTARA masdingin naipospos; Muhammad Idris; Rahmadina Rahmadina
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 2 (2022): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i2.11687

Abstract

Sembung [Blumea balsamifera (L.) DC] is a medicinal plant that contains secondary metabolites. Differences in geographical location, climate, morphology and plant parts used can affect the content of secondary metabolites and the levels of flavonoids produced. The purpose of this study was to analyze the presence of secondary metabolites and flavonoid levels in sembung leaf extract from Hasang and Simangalam villages. The method used is to observe and observe samples in the field and identify the presence of secondary metabolites and flavonoid levels through laboratory tests. Extraction is done by the maceration method. Flavonoid testing was carried out qualitatively (phytochemical screening test, KLT test) and quantitative (Test levels with UV-Vis spectrophotometry ). The results showed that sembung plants from Hasang and Simangalam villages were positive for flavonoids, saponins and steroids. The KLT test for flavonoid compounds in Hasang village on N-hexane : ethyl acetate resulted in 3 spots and on Chloroform : ethyl acetate  8 spots for flavonoid compounds. Extract from Simangalam village on N-hexane : ethyl acetate resulted in 8 spots and Chloroform : ethyl acetate produced 19 spots for flavonoid compounds.  The result showed that the flavonoid content of sembung leaf extract from Hasang village was 89,2971 mg QE/g, which was higher than the flavonoid content from Simangalam village was 83,9828 mg QE/g.
FRUGIPERDA) TANAMAN JAGUNG DI DESA MLATIREJO BULU REMBANG Afina Tur Rohmah
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 2 (2022): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i2.12645

Abstract

Ulat grayak atau Fall Armyworm (Spodoptera frugiperda J. E. Smith) merupakan hama baru pada tanaman jagung di Indonesia. Hama tersebut berasal dari benua Amerika dan telah menyebar ke berbagai wilayah Afrika dan Asia. Hama ini termasuk sulit untuk dikendalikan, karena penyebaran imago sangat cepat, dan termasuk penerbang yang sangat kuat. Kehilangan hasil yang ditimbulkan oleh kerusakan serangan Spodoptera frugiperda mencapai 40%. Maka diperlukan suatu tindakan untuk mengendalikan hama tersebut salah satunya yaitu dengan menggunakan insektisida. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian insektisida Ammate dengan pemberian dosis yang berbeda terhadap tingkat kematian hama ulat grayak Spodoptera frugiperda pada tanaman jagung. Penelitian ini menggunakan rancangan acak lengkap dengan tiga konsentrasi perlakuan yaitu 5%, 10%, 15% dan satu larutan kontrol yaitu 0%. Data hasil penelitian dianalisis menggunakan aplikasi SPSS versi 22. Hasil penelitian menunjukkan mortalitas yang berbeda pada konsentrasi 0% digunakan sebagai larutan kontrol tidak menunjukkan adanya mortalitas, pada konsentrasi larutan 5% menunjukkan rata-rata mortalitas sebanyak 44%, pada konsentrasi 10% menunjukkan rata-rata mortalitas sebanyak 78%, sedangkan pada konsentrasi larutan 15% menunjukkan rata- rata mortalitas sebanyak 89%, perlakuan dengan konsentrasi larutan 10% dan 15% menunjukkan hasil yang paling signifikan yaitu dapat mematikan 50% dari ulat uji.
Hubungan Kadar Glukosa Darah dan Glukosa Urin dengan Terjadinya Infeksi Saluran Kemih (ISK) Dengan Menggunakan Media Alternatif Pada Penderita Diabetes Melitus di UPTD Diabetes Center Kota Ternate Erpi Nurdin; Nikma Nikma; Dwiana Marta Risfiantika
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 2 (2022): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i2.13582

Abstract

Diabetes mellitus is a metabolic disease characterized by hyperglycemia resulting from defects in insulin secretion, insulin action or both. Blood glucose and urine glucose levels will increase in a history of diabetes mellitus, the accumulation of glucose in the urine is a medium for microbes to multiply, which can cause urinary tract infections in patients with diabetes mellitus. Alternative media of tuna extract agar, able to grow bacteria on gram positive and negative bacteria isolates. The aim is to determine the relationship between blood glucose and urine glucose levels on UTI-causing bacterial cultures in urine using tuna alternative media. The research method is descriptive with a comparative approach. Based on the research that has been carried out, the results are described that identified 100% increased blood glucose levels, 63 respondents with high blood glucose levels accompanied by positive results of urine culture on alternative media, and there were 29 respondents with positive urine glucose levels accompanied by positive results of bacterial culture causes of UTI in urine. The conclusion is that an increase in blood and urine glucose can lead to urinary tract infections, even though they are very weak. The p value is 0.036 which states that the correlation between blood glucose levels and the results of UTI-causing bacteria culture in urine using alternative media, namely the strength of the correlation is very weak (0.0 - <0.2). The p value is 0.014 which states that the correlation between urine glucose levels and urine culture results using alternative media, namely the strength of the correlation is very weak (0.0 - <0.2).
MINI REVIEW: PENGEMBANGAN BIOSENSOR KOLORIMETRI BERBASIS AGREGASI NANOPARTIKEL EMAS Hana Verdiani; Dinda Syafira; Husna Nugrahapraja
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.14657

Abstract

Gold nanoparticle (AuNP) is a material commonly used in colorimetric detection for biological sensors due to its low production cost, simplicity, and ease use. AuNP is known to generate different colors depending on the particle aggregation level. In the last two decades, changes in AuNP aggregation status have become the basis for developing a sensor to detect analytes in the sample. The solution color changes could be observed without sophisticated equipment, allowing ,more straightforward interpretation of the detection result. This review focuses on developing AuNP aggregation-based colorimetric tests for detecting various biological analytes. It discusses multiple mechanisms, strategies, functionalization, the latest research, and perspectives of AuNP use in colorimetric detection, which could be considered for future biosensor development. The concept of colloidal gold nanoparticle aggregation and stabilization is emphasized in this review. 
DETEKSI CEMARAN Salmonella sp. PADA KOMODITAS TUNA, TONGKOL DAN CAKALANG (TTC) YANG DIJUAL DI PASAR INPRES DI DAERAH ISTIMEWA YOGYAKARTA Ratnaningtyas, Susi; Wahyudi, Dzikri; Wulansari, Devi; Utami, Wayah Putri
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 2 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.13622

Abstract

Fish is a nutrition source, mainly protein, which is easily decomposed as a result of bacterial and enzyme activity. Therefor its fish after harvest if it is not done properly will cause the fish to become damaged quickly and experience a decrease in quality because fish is a type of food that is easily subject to biological damage and is susceptible to microbial contamination (Christanti and Azhar, 2019). Incorrect handling will lead to decreased product quality. Cross contamination is one result of poor handling processes, so that the product is contaminated by pathogenic bacteria. One of the pathogenic bacteria, namely Salmonella sp. which needs to be considered for its presence in fishery products, both fresh and frozen (Ijong, Berhimpon and Sumampow, 2015). The purpose of this research is to determine the contamination of Salmonella sp. and the prevalence of contamination in Tuna, Cob and Skipjack (TTC) commodities. Final Practical Work (KPA) will be held from 14 March to 10 June 2022 at the Fish Quarantine Station, Quality Control and Safety of Fishery Products in Yogyakarta, located in Sleman Regency, Yogyakarta. The samples tested were 19 fresh fish products in the Tuna, Cob and Cakalang (TTC) commodities. Microbiological quality testing of fishery products, both fresh and frozen, needs to be carried out in order to guarantee food safety for the community. Test procedure for Salmonella sp. using conventional methods in accordance with SNI 01-2332.2-2006, which includes pre-enrichment, enrichment, isolation of bacteria or selective, observation of typical Salmonella colony morphology, isolation of pure culture of Salmonella and biochemical tests. The test results showed that all fresh fish samples taken from the InPres market in the Yogyakarta area were negative for Salmonella sp. and the prevalence is 0%. The application of good sanitation and hygiene will prevent contamination of pathogenic bacteria and still maintain the cold chain to inhibit enzyme reactions found in the fish's body. Bacterial contamination Salmonella sp. usually found in fishery products, agriculture and food products
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 70% KLIKA TURI (Sesbania grandiflora L.) TERHADAP Staphyloccous epidermidis DAN Propionibacterium acnes Andi Dian Astriani
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.13519

Abstract

Acne is a skin disease that occurs due to bacterial infection, usually on the skin surface of the face, neck, chest, and back. The Turi plant is one plant with medicinal properties that can be used as an antibacterial. This study aims to determine the antibacterial activity of 70% ethanol extract of klika turi against Staphylococcus epidermidis and Propionibacterium acnes. Klika turi was extracted by reflux method using 70% ethanol solvent. They were testing the antibacterial activity of 70% ethanol extract against Staphylococcus epidermidis and Propionibacterium acnes using liquid dilution and agar diffusion methods. The results showed that the MIC values were 2.5% against Staphylococcus epidermidis and 5% against Propionibacterium acnes. Activity test with the diffusion method to obtain the diameter of the inhibition zone against Staphylococcus epidermidis at an extract concentration of 2.5%; 5%; 10%; and 20%, which is 7.35 mm; 8.67mm; 11.08 mm and 11.55 mm, while for Propionibacterium acnes at an extract concentration of 5%; 10%; and 20% obtained the diameter of the inhibition zone which is 8.77; 11.32mm; and 12.00mm. The results can be concluded that the 70% ethanol extract of klika Turi has effective antibacterial activity at a concentration of 10% against Staphylococcus epidermidis and Propionibacterium acnes.).
ISOLASI DAN KARAKTERISASI BAKTERI AMILOLITIK DARI SAMPEL AIR PANTAI PARIS TIGARAS Vila Tria Putri; Rasyidah Rasyidah; Ulfayani Mayasari
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.12880

Abstract

Amylolytic bacteria have great genetic diversity with high enzymatic activity and have the expertise to break down starch into simpler materials and degrade organic substances in water. This study aims to determine the variety of types of amylolytic bacteria obtained and to see how the characterization of bacteria is isolated from the waters of Paris Tigaras Beach. Isolation of amylolytic bacteria from the waters of Paris Tigaras Beach was carried out using the spread plate method taken from the last dilution of 10-5. Isolates ST3 03 and ST3 04 showed amylolytic activity by showing a clear zone of 11.8 mm in isolates ST3 03 and 8.2 mm in isolates ST3 04. Characterization of isolates ST3 03 and ST3 04 was observed including Gram-positive bacteria with rod-shaped cells, that can produce catalase enzymes, ferment three types of sugars, and move (motil). From the results of observations of the morphology of bacterial colonies and cells, and biochemical tests, isolates of amylolytic bacteria ST3 03 and ST3 04 belong to the genus Bacillus.
Uji Aktivitas Ekstrak Akar Nipah (Nypa fruticans) Sebagai Antimikroba Staphylococcus aureus ATCC 25923 Dan Candida albicans ATCC 10231 Nia Agustina; Mauritz Pandapotan Marpaung
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.14059

Abstract

Nipah fruticans adalah sejenis palem yang mengandung senyawa metabolit sekunder yang berpotensi memiliki berbagai efek biologis, seperti antibakteri dan antijamur. Penelitian ini bertujuan menganalisis ekstrak akar nipah dapat menghambat pertumbuhan Staphylococcus aureus ATCC 25933 dan jamur Candida albicans ATCC 10231. Akar nipah diekstraksi dengan metode maserasi dalam pelarut etanol 96%. Skrining fitokimia dilakukan untuk mengindentifikasi senyawa metabolit sekunder. Uji mikroba dengan metode Difusi Agar dengan konsenterasi ekstrak 10%, 20%, 30%, 40%, 60%, 80%, dan 100%. Kontrol positif untuk uji antibakteri adalah klindamisin dan ketokonazol untuk uji antijamur. Hasil penelitian yaitu adanya metabolit sekunder berupa flavonoid, alkaloid, saponin, steroid, dan terpenoid. Diameter hambat ekstrak pada uji bakteri terdapat pada ekstrak 100%, sedangkan pada uji jamur, tidak ditemukan adanya daya hambat.
Kajian Etnobotani Tanaman Hanjuang (Cordyline fruticosa) pada Wawatjan Babad Sumedang Fauzan Ahmad Wijaya; Tri Cahyanto
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.14589

Abstract

Wawatjan Babad Sumedang is one of the ancient texts telling about the struggle of Prabu Geusan Ulun in defending and taking responsibility for Sumedang from the Cirebon attack as a result of the love between him and Harisbaya, the empress of Cirebon. In one part of this chronicle, the Hanjuang was planted by a character named Embah Djaja Perkosa. It is the background for the study of the manuscript from an ethnobotanical perspective. This study uses qualitative methods with data collection techniques through interviews, observation, and literature studies. Interviews were conducted in a semi-structured and snowball-sampling manner with community leaders. Furthermore, observation was carried out by visiting the Hanjuang Kutamaya complex in Padasuka Village, North Sumedang District, Sumedang Regency, West Java. While the literature study was performed on related references, especially the Wawatjan Babad Sumedang Manuscript. Hanjuang is mentioned in the text in verses 148, 149, 171, and 172. Hanjuang itself is a siloka of "hayu urang berjuang" or "let's fight" . In the written text, the word ngalakai is a sign that the Hanjuang needs an appropriate environment to live in. This suitable environment is filled with the flourishing of Kutamaya thanks to the existence of the river there. In addition, the process of planting Hanjuang in the events in the script is done by stem cuttings. However, the Hanjuang side as an indicator has not been resolved in this study, so further research is needed on this matter

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