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Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
Published by Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medis Indonesia
ISSN : 27750108     EISSN : 27742504     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology
Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) merupakan jurnal AIPTLMI (Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia) yang dapat diakses secara terbuka (open acces) yang berfokus pada pengembangan ilmu pengetahuan Teknologi Laboratorium Medik (TLM) dengan tujuan untuk memperluas pengetahuan, pemikiran dan penelitian di bidang laboratorium kesehatan. Topik yang diangkat meliputi hematologi, kimia klinik, imunoserologi, imunohematologi, sitohitoteknologi, mikrobiologi, parasitologi, dan toksikologi klinik.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 82 Documents
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PENGUJIAN AKTIVITAS ANTIMIKROBA EKSTRAK ETIL ASETAT DAN METANOL KAYU RANTING SENGON (Falcataria moluccana) SAKIT Popy Listiani; Popy Hasanah; Alfi Rumidatul; Feldha Fadhila; Yayan Maryana
Journal of Indonesian Medical Laboratory and Science Vol 2 No 1: April 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i1.23

Abstract

Klebsiella pneumoniae, Shigella dysenteriae, Salmonella typhi, Escherichia coli, and Candida albicans are the microorganisms that most often infect. Infectious diseases can be cured and the transmission is minimized using various kinds of antibiotics.The search for antimicrobial compounds from nature is expected to reduce the negative effect of antibiotics. One of the plants suspected of having antimicrobial content is the sengon tree. This study aims to determine the ability of sengon sore wood extract with ethyl acetate and methanol as an antimicrobial solvent. The test method used is Kirby bauer with treatments 9%, 9.5%, 10%, 10.5%, and 11%. The result of this study showed that the ethyl acetate extract of sengon sick wood showed antimicrobial activity against Shigella dysenteriae at all concentrations and in Klebsiella pneumoniae only at a concentration of 11%, there’s no antimicrobial activity against E. coli. A Methanol extract of sengon sick twig wood showed antimicrobial activity against Klebsiella pneumoniae at concentrations of 10%, 10.5%, 11%, and on Candida albicans at all concentrations, but didn’t show antimicrobial activity against E. coli, S. typhi, and S. dysenteriae. Can be concluded that the ethyl acetate extract of sengon sick wood branches could inhibit the growth of Shigella dysenteriae and Klebsiella pneumoniae. Meanwhile, methanol extract of sengon sick twig wood can inhibit the growth of Klebsiella pneumoniae and Candida albicans.
ANALISA EKSTRAK ETIL ASETAT AKAR KAIK-KAIK (Uncaira cordata (Lour.) Merr.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus Reza Amelia; Riky; Febri Nur Ngazizah
Journal of Indonesian Medical Laboratory and Science Vol 2 No 1: April 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i1.24

Abstract

The people of Kalimantan use many herbs for treating diseases, one of them is akar kaik-kaik (Uncaira cordata (Lour.) Merr.) The plant has a high concentration of alkaloids, phenolic, steroids, terpenoids, and flavonoids. that act as antibacterias using diffusion method so the Kirby Bauer/disk paper method used to define antibacterial activity marked the inhibition zone for S. aureus, which is a spherical positive Gram bacterium. U. cordata cordata was extracted through multilateral extraction by n-hexane solution followed by ethyl acetate. The extract used in this study was ethyl acetate with 5 variations of concentration treatment (100 mg/1, 200 mg/1, 300 mg/1, 400 mg/1, 500 mg/1). This research was an experiment design and analyzed by One Way ANOVA test, the mean score for inhibition zone for U. cordata ethyl ecetate extracts in 100 mg/L concentration was 12.7 mm under the weak category, for the concentration of 200 mg/L was 14.2 mm in the weak category, for the concentration of 300 mg/L was 15.3 mm in the weak category, for  the concentration of 400 mg/1 was 16.3 mm in the medium category and for the concentration of 500 mg/1 was 17.7 mm in the medium category.
PEMANFAATAN KUBIS UNGU (Brassica oleracea L) SEBAGAI INDIKATOR FERMENTASI KARBOHIDRAT PADA MEDIA UJI BIOKIMIA Rochmanah Suhartati; Dewi Peti V; Fanzi Afsgar
Journal of Indonesian Medical Laboratory and Science Vol 2 No 1: April 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i1.32

Abstract

Purple cabbage (Brassica oleracea L) contains anthocyanins that are soluble in 96% ethanol. These anthocyanin compounds can change color at acidic, neutral and alkaline pH. The color change can be used as an indicator of carbohydrate fermentation reaction in the biochemical test media for mannitol and lactose by utilizing ethanol extract of purple cabbage as an alternative acid-base indicator in liquid sugar medium. The research method used is experimental. The results showed that the purple cabbage extract solution could be used as an alternative acid-base indicator to see the results of the fermentation reaction of mannitol and lactose in the medium. The color change that occurs is from colorless to pink. Based on the results it can be concluded that the ethanol extract of purple cabbage can be used as a color indicator in the mannitol dan lactose carbohydrates fermentation test.
PERBANDINGAN LARUTAN FIKSATIF NAFS DENGAN ALKOHOL 96%-NBF 10% DALAM PEMBUATAN BLOK SEL PADA SAMPEL CAIRAN PLEURA Dewi Inderiati; Bella Eka Pratiwi
Journal of Indonesian Medical Laboratory and Science Vol 2 No 1: April 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i1.40

Abstract

Pleural fluid is a liquid located in the pleural cavity, between the pleural visceral and pleural parietal. Analysis of pleural fluid is mandatory in all cases of diseases that give rise to pleural effusion. Cytopathological test gives the first indication of malignancy in malignant effusion, one of which is by making cell block. The stages of making cell block begin with fixation to maintain the cell component and its state is close to the original. The purpose of this study was to determine the comparison of the quality of the cytopathological examination results from the manufacture of cell block using a fixative solution of Nathan Alcohol Formalin Substitute (NAFS) and alcohol 96% -NBF 10% in pleural fluid samples. Experimental research with cross-sectional research design. The results showed that 13 samples of pleural fluid preparations in cell block preparations with NAFS fixative solution and cell block preparations with 96%-NBF 10% alcohol fixative solution there was no significant difference in the morphological figure of the cells, the number of cells produced, the number of malignant cells analyzed, the absorption of staining and cytodiagnosis sensitivity. Based on the results of this study, it can be concluded that alcohol 96% -NBF 10% can be used as an alternative solution to fixative for pleural fluid samples.
PENGARUH PEMBERIAN SUSU KEDELAI TERHADAP PROFIL LIPID WANITA MENOPAUSE Rianti; Meti Kusmiati
Journal of Indonesian Medical Laboratory and Science Vol 2 No 1: April 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i1.42

Abstract

Soy milk is the result of extraction from soybeans. Soy milk protein has an amino acid composition similar to cow's milk. Soy milk can be used as a source of nutrition to improve nutritional quality, especially for menopausal women. Menopause is a period when a woman no longer has menstruation because her estrogen hormone production stops. After menopause there are changes in lipid metabolism, which is a risk factor for atherosclerosis. This study aims to determine the effect of soy milk consumption on lipid profiles in menopausal women. The sampling method used was purposive sampling. The number of samples taken was 15 menopausal women. Samples were taken before and after the respondents consumed 200 ml of soy milk every morning and evening for 14 days. The parameters measured include total cholesterol, HDL cholesterol (High Density Lipoprotein), LDL cholesterol (Low Density Lipoprotein) and triglycerides. The statistical results using the Paired T test showed that the significance value for total cholesterol was 0.248, HDL cholesterol 0.000, and LDL cholesterol 0.022 while for triglycerides the significance value was 0.496, So it can be concluded that there is a significant difference in the mean levels of HDL cholesterol and LDL cholesterol but there is no significant difference in total cholesterol and triglyceride levels between before and after giving soy milk to menopausal women.
KORELASI ANTARA KADAR HbA1c DENGAN LAJU FILTRASI GLOMERULUS (LFG) PADA PASIEN DIABETES MELITUS Retno Martini Widhyasih; Ria Nur Puspa Sari; Bagya Mujianto
Journal of Indonesian Medical Laboratory and Science Vol 2 No 1: April 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i1.45

Abstract

Diabetes mellitus (DM) is a metabolic disorder characterized by prolonged hyperglycemia with a risk of complications in the kidney. Glycemic control can be monitored by means of an HbA1c test. Poor glycemic control can lead to decreased kidney function which can be assessed from the glomerular filtration rate (GFR). GFR is measured using serum creatinine levels using the equation Modification of Diet in Renal Disease (MDRD). This study aims to determine the correlation between HbA1c levels and LFG in DM patients. The research method was observational analytic with cross sectional design on 90 medical records of DM patients in 2019 at Budhi Asih Hospital, which carried out HbA1c and serum creatinine tests as the basis for calculating LFG. Data analysis used Spearman's correlation test with a 95% CI (? = 0.05). The results showed that the average HbA1c level was 7.9%, meaning that most patients had bad HbA1c levels and the average LFG result was 60 mL/min/1.73m2, meaning that most patients had an LFG value <90 mL/min/1.73m2 and indicates a disturbance in renal function. The results of Spearman's correlation show a negative direction (r) of -0.396. It can be concluded that the higher the HbA1c level, the smaller the LFG value so that DM patients must carry out regular glycemic control to prevent a decrease in kidney function which can lead to complications.
UJI DAYA HAMBAT EKSTRAK BAWANG DAYAK (Eleutherine palmifolia (L.) Merr.) TERHADAP BAKTERI Salmonella parayphi Nor Aini Aida; Febri Nur Ngazizah; Miftachul Sobirin; Riky Riky
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.48

Abstract

Dayak onions (Eleutherine palmifolia (L.) Merr.) Is one of the natural ingredients which is often used for medicine in Kalimantan. E.  palmifolia used for treatment because it contains secondary metabolite compounds that have potential as antibacterial. Such as phenolic compounds, flavonoids, alkaloids, saponins, tannins and glycosides which are included in polar compounds. This study aims to determine the effect of ethanol extract 70% E. palmifolia against bacteria Salmonella paratyphi with the extraction method maceration. S. paratyphi is a bacterial pathogen against humans whose habitat is in the digestive tract. This research was conducted to test the extract's antibacterial activity with gradient concentration of 20 mg / mL, 30 mg / mL, 40 mg / mL, 50 mg / mL, and 60 mg / mL and chloramphenicol as a positive control and sterile aquadest as a negative control.  The antibacterial test method used is diffusion method with 5 repetitions. Our results showed that E. palmifolia extract could inhibit S. paratyphi growth as evidenced by the presence of an inhibition zone that is formed around paper disk on media with concentration 20 mg / mL, 30 mg / mL, 40 mg / mL, 50 mg / mL, and 60 mg / mL. Where the average diameter of the inhibition zone was 6.6 mm, 7.4 mm, 8.8 mm, 9.9 mm, respectively fall into the category of ineffective inhibition and 11 mm is in the weak category. Based on the research that has been done, it was found that there was an effect of giving E. palmifolia extract on the growth of S. paratyphi bacteria but there was no significant difference based on the one way ANOVA test.
ANALISA EKSTRAK ETANOL DAUN BAJAKAH KAIT-KAIT (Uncaria acida (Hunt.) Roxb.) TERHADAP BAKTERI Escherichia coli MENGGUNAKAN METODE KIRBY BAUER Mia Irawan; Febri Nur Ngazizah; Riky Riky; Iqlila Romaidha
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.49

Abstract

Kalimantan forest is the largest tropical rain forest which is one of the places with biodiversity that has the potential for development in the field of health or medicine, one of the plants used for treatment is hook root (Uncaria acida (Hunt.) Roxb.). This plant has indole alkaloid compounds, triterpenes, flavonoids and phenylpropanoids that act as antibacterial. In this study, an antibacterial test was conducted using the Kirby Bauer method to determine the antibacterial activity which was characterized by the formation of an inhibition zone around the paper disc. The bacteria tested were E. coli. The extract tested in this study was the ethanolic extract of U. acida leaves with 5 concentrations of treatment (5 mg/mL, 15 mg/mL, 30 mg/mL, 40 mg/mL and 50 mg/mL). The average result of the inhibition zone of the ethanolic extract of U. acida leaves in a concentration of 5 mg/mL is 8.1 mm in the weak category, the concentration of 15 mg/mL is 8.3 mm in the weak category, the concentration of 30 mg/mL is 9.1 mm in the weak category, 40 mg/mL ie 9.2 mm in the weak category and 50 mg/mL in the moderate category. Using significance (? > 0.05) which means that the inhibition zone data on the growth test of E. coli bacteria at the 5 concentrations affected the inhibition zone of E. coli bacteria.
PERBANDINGAN EFEKTIVITAS EKSTRAK BAWANG PUTIH (Allium sativum L.) DENGAN DAUN BAJAKAH (Uncaria acida Roxb.) SEBAGAI LARVASIDA VEKTOR DENGUE Aedes aegypti Fitra Akbar Mulyono; Iqlila Romaidha; Nur Aini Hidayah Khasanah; Febri Nur Ngazizah
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.50

Abstract

Dengue fever is a disease caused by the dengue virus carried by the A. aegypti mosquito. Making natural larvicides from plants such as Alium sativum L. and Uncaria acida Roxb. are indispensable. This study aims to make natural larvicides from A. sativum and U. acida. This study is an experimental study using a Completely Randomized Design (CRD) to see the effectiveness of the extract A. sativum with U. acida as larvicide of the mosquito A. aegypti.This study used 20 mosquito larvae for each concentration and repeated 4 times. The results showed that the larvicide of A. sativum at the highest concentration reached 100% mortality, while the larvicide of U. acida at the highest concentration reaching 80% mosquito larvae death A. aegypti. Analysis of research data was processed using SPSS version 20. One Way Anova analysis of A. sativum extract was sig 0.049 and U. acida extract was sig 0.245. In the extract of A. sativum there was a significant difference in the mortality of mosquito larvae at each concentration, while in the extract of U. acida there was no significant difference in the mortality of mosquito larvae at each concentration. T-test analysis of the extracts of A. sativum and U. acida was sig 0.80, so there was no difference in effectiveness between the two larvicidal extracts. It can be concluded that the larvicidal research results of A. sativum extract and U. acida extract were equally effective on A. aegypti larvae.
PERBEDAAN DARAH SEGERA DIPERIKSA, DILAKUKAN PENYIMPANAN PADA SUHU 20ºC-25ºC DAN 4ºC-8ºC SELAMA 6 JAM TERHADAP JUMLAH ERITROSIT Ferdi Afriansyah; Bastian Bastian; Indah Sari; Denny Juraijin
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.51

Abstract

Errors that often occur in laboratory examinations are in the pre-analytic stage with an error percentage of 50%-75%. Examination of blood samples to maintain conditions so that they are not damaged, the samples are usually stored at a temperature 4-8oC for several hours to 4-5 days and blood specimens stored temperature of 20-24oC for a maximum examination 24 hours. However, the examination of the number of erythrocytes should be carried out immediately after collection without storage because the delay in examination time can affect the number of erythrocytes, the longer the delay, the number of erythrocytes decreases because the cells undergo hemolysis, biochemical, biomechanical and immunological reactions. This researcher aims to determine the difference in blood immediately examined, blood stored temperature of 20oC-25oC and 4oC-8oC for 6 hours on the number of erythrocytes. The research design used a true experiment with a pre-test design and a post-test control group design with a hypothesis test using the Two-way ANOVA test. This research was conducted 30 respondents of DIV Medical Laboratory Technology students. The results of the study the number of erythrocytes in the blood immediately with an average value of 4.57 million/µI, at a temperature of 20oC-25oC has an average value of 4.65 million/µL, temperature of 4oC-8oC has an average value of 4.50 million/µL and Friedman test obtained p value = 0.172. Conclusion of the study showed that there was no difference in blood immediately examined, the blood was stored at a temperature of 20oC-25oC and 4oC-8oC for 6 hours on the number of erythrocytes.

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