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Ummy Mardiana Ramdan
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Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
Published by Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medis Indonesia
ISSN : 27750108     EISSN : 27742504     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology
Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) merupakan jurnal AIPTLMI (Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia) yang dapat diakses secara terbuka (open acces) yang berfokus pada pengembangan ilmu pengetahuan Teknologi Laboratorium Medik (TLM) dengan tujuan untuk memperluas pengetahuan, pemikiran dan penelitian di bidang laboratorium kesehatan. Topik yang diangkat meliputi hematologi, kimia klinik, imunoserologi, imunohematologi, sitohitoteknologi, mikrobiologi, parasitologi, dan toksikologi klinik.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 84 Documents
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The The Effect of Blood Transfusion Frequency on Secretor Status Titer in Saliva and Urine Specimens of Thalassemia Patients Darmantoputro, Feby Wicaksono; Nurhayati, Betty; Hayati, Eem; Marliana, Nina
Journal of Indonesian Medical Laboratory and Science Vol 6 No 2: Oktober 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i2.308

Abstract

Background: Thalassemia is a disease caused by a deficiency or loss of synthesis of one or more normal globin chains. In diseases that require blood transfusions, such as Transfusion Dependent Thalassemia (TDT), it is very important to examine the blood type to determine the blood to be transfused. The ABO blood type system antigen or secretor, is found in body fluids in the form of dissolved antigens, including in saliva and urine. Interference can be caused by the frequency of transfusion, which can change the secretor content in the saliva and urine of thalassemia patients, causing false negatives in the examination of secretor status. Objectives: This study aims to determine the effect of transfusion frequency on the titer of secretor status in saliva and urine specimens of Thalassemia patients. Materials and Methods: The type of study is quasi-experimental, Agglutination-Inhibition method. The research sample was 23 Thalassemia major patients. 15 patients are secretor patients, with a frequency of 1x transfusion a month were 7 people (47%), a frequency of 2x transfusion a month were 5 people (33%), and 4x transfusion a month were 3 people (20%). Results: The results of the study on Thalassemia patients obtained from saliva were titers between 1/8 to 1/256. From urine, the results were non-secretors. In contrast to previous studies, which stated that urinary is still detected but four times weaker than saliva. Based on the ANOVA statistical calculation, sig. 0.909 (p>0.05) was obtained. Conclusions: The conclusion is that there is no effect of transfusion frequency on the titer of secretor status in saliva or urine.
Correlation Between Leukocyte Count and Erythrocyte Sedimentation Rate in Pulmonary Tuberculosis Patients at Suai Referral Hospital, Timor Leste Gunawan, Lucia Sincu; Livrança, Expedito José Oliveira; Ismawatie, Emma
Journal of Indonesian Medical Laboratory and Science Vol 6 No 2: Oktober 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i2.320

Abstract

Background: Human Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. TB causes serious health problems for about 10 million people each year and is one of the most common causes of death worldwide. The incidence of TB in Indonesia is 354 per 100,000 population, while in Timor-Leste the incidence has stagnated at 498 per 100,000 population. An active TB process may be indicated by an increase in leukocytes and LEDs, whereas an improvement is shown by a decrease in LEDs and normal lecocyte counts. Objectives: This study aimed to determine the correlation between leukocyte count and erythrocyte sedimentation rate (ESR) in pulmonary TB patients at Suai Referral Hospital, Timor-Leste. Materials and Methods: This was an observational analytic study with a cross-sectional approach, conducted from April to June 2023, using medical laboratory records. The study population consisted of pulmonary TB patients who underwent laboratory testing, with a total sampling of 50 patients. Data on leukocyte counts measured using an automated Hematology Analyzer and ESR determined by the Westergren method were tested for normality using the Shapiro-Wilk test, followed by Spearman’s rank correlation test.  Results: The mean leukocyte count was 11.15 ± 5.24 /mm³ and the mean ESR was 63.30 ± 35.71 mm/hour. The correlation test between leukocyte count and ESR showed r = 0.124 (p = 0.392), indicating no significant correlation. Conclusions: There is no significant correlation between leukocyte count and ESR in pulmonary TB patients at Suai Referral Hospital, Timor-Leste.
Utilization of neem leaf extract (Azadirachta indica A. Juss.) in inhibiting the growth of acne-causing bacteria Cutibacterium acnes Sinatra, Dhimas Pramayoga; Rini, Chylen Setiyo
Journal of Indonesian Medical Laboratory and Science Vol 7 No 1: April 2026
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v7i1.284

Abstract

Background: Acne is an infection that occurs on the surface of the skin in the form of inflammation of the oil glands in the epidermal layer of the skin, when infected by the bacteria Cutibacterium acnes, blackheads will develop into inflammation and cause acne vulgaris. One of the plants that can be used as a natural alternative in acne treatment is neem (Azadirachta indica A. Juss). Objectives: This research aims to identify the inhibitory effect of neem leaf extract on C. acne. Materials and Methods: Neem leaves samples were obtained from the Sumenep district. The research was conducted at the PIPOT-UBAYA Laboratory, the Pharmacy Laboratory of PGRI Adi Buana University Surabaya and the Microbiology Laboratory of Stikes Ngudia Husada Madura. The research design used is experimental, testing of the inhibition power of neem leaves using the diffusion method by observing the inhibition zone around the disc. This research used concentrations of 25, 50, 75, and 100%. The positive and negative controls used are sterile aquades and Clindamycin. Results: The results showed the inhibition zones of neem leaf extract against C. acnes at concentrations of 25% and 50% were 6.3 mm and 9.3 mm, while at concentrations of 75% and 100% were 12.3 mm and 16 mm. Conclusions: The results of the one-way ANOVA test showed p-value is 0.000, which means that neem leaves can inhibit the growth of C. acnes.
Parijoto (Medinilla speciosa) extract as a natural dye alternative for peripheral blood smears: A comparative study with giemsa stain Trisna, Meida; Indanah, Indanah; Rusidah, Yunita; Saputro, Arief Adi; Sholikhati, Anisa; Ekawati, Sherly Nur
Journal of Indonesian Medical Laboratory and Science Vol 7 No 1: April 2026
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Peripheral blood smear staining is an essential hematological procedure used to evaluate blood cell morphology. The commonly used standard stain is Giemsa stain; however, its chemical components may pose potential risks to health and the environment. Therefore, safer natural alternatives are needed. Previous studies have reported that anthocyanin-containing plant extracts, such as Hibiscus sabdariffa, Tectona grandis, and Garcinia mangostana, can effectively stain blood cells. Medinilla speciosa (parijoto) also contains anthocyanins and has potential as a natural dye. Objective: This study aimed to evaluate the staining quality of peripheral blood smears using Medinilla speciosa extract at different concentrations and to compare the results with a 10% Giemsa stain control. Methods: This laboratory-based experimental study used a post-test only control group design. A total of 30 blood smears were stained using Medinilla speciosa extract at concentrations of 25%, 50%, and 70%, along with a 10% Giemsa stain control. The extract was prepared using maceration with pH adjustment to optimize anthocyanin stability. Staining quality was assessed based on color intensity, background clarity, and erythrocyte morphology using a semi-quantitative scoring system. Data were analyzed using the Kruskal–Wallis test and Mann–Whitney U test. Results: Increasing extract concentration was generally associated with improved staining quality. A significant difference among groups was observed (p = 0.002). Pairwise analysis showed that the 70% extract was not significantly different from the 10% Giemsa stain control (p = 0.321), while lower concentrations differed significantly. Conclusion: The 70% Medinilla speciosa extract showed the best performance among extract groups and demonstrated comparable staining quality in certain parameters. However, Giemsa stain remains the more consistent standard. Further optimization is required before routine application.

Page 9 of 9 | Total Record : 84

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