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Hanif Amrulloh
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Journal of Multidisciplinary Applied Natural Science
Published by Pandawa Institute
ISSN : -     EISSN : 27743047     DOI : 10.47352/jmans
Core Subject : Science, Education, Agriculture, Social, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics
Journal of Multidisciplinary Applied Natural Science (abbreviated as J. Multidiscip. Appl. Nat. Sci.) is a double-blind peer-reviewed journal for multidisciplinary research activity on natural sciences and their application on daily life. This journal aims to make significant contributions to applied research and knowledge across the globe through the publication of original, high-quality research articles in the following fields: 1) biology and environmental science 2) chemistry and material sciences 3) physical sciences and 4) mathematical sciences. The J. Multidiscip. Appl. Nat. Sci. is an open-access journal containing original research articles, review articles, and short communications in the areas related to applied natural science. The J. Multidiscip. Appl. Nat. Sci. publishes 2 issues in a year on January (first issue) and July (second issue). This journal has adopted a double-blind reviewing policy whereby both the referees and author(s) remain anonymous throughout the process.
Arjuna Subject : Umum - Umum
Articles 165 Documents
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Three Decades of Sunda Pangolin (Manis javanica) Conservation Research: Mapping Indonesia’s Role in the Global Engagement Trends Novriyanti, Novriyanti; Masy’ud, Burhanuddin; Soekmadi, Rinekso; Buchori, Damayanti
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.361

Abstract

Limited scientific attention to the Sunda pangolin (Manis javanica) persists despite being among the most trafficked mammals globally and a key species distributed across Indonesia. This study examines Indonesia’s contribution to global Sunda pangolin research and identifies priority directions to strengthen evidence-based conservation. A combined bibliometric–content analysis approach was applied to 4,474 publications recorded between 1993–2023, from which 130 articles met the topic-specific criteria, and 37 were directly related to Sunda pangolin conservation in Indonesia. Results show that Indonesia contributed only 37 documents (0.83%) to the global pangolin research landscape, with 12 thematic categories dominated by trade and crime studies, while ecological and policy-oriented research remained critically underrepresented. Research on Sunda pangolins was also largely driven by international authors from non-habitat countries, indicating a lack of local leadership and collaboration gaps. These findings highlight a strategic need to increase locally led and multidisciplinary research, expand Indonesia’s role as a key habitat country, and establish collaborative research agendas that align ecological knowledge, conservation policy, and wildlife crime mitigation.
Hoof Pathologies in Cattle Kulynych, Serhii; Klymas, Anastasiia; Shabalin, Oleksandr; Zvenihorodska, Tamila; Petrenko, Maksym; Kolomak, Ihor; Tul, Oleksandra; Slynko, Viktor; Avramenko, Nataliia; Panasova, Tetiana; Omelchenko, Hanna
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.363

Abstract

Hoof pathologies are among the most widespread orthopedic disorders in dairy cattle and are a major contributor to reduced animal welfare and productivity. The present study investigated the prevalence, clinical presentation, and treatment outcomes of the most common hoof lesions – digital dermatitis (Mortellaro’s disease), Rusterholz ulcers, purulent pododermatitis, and interdigital dermatitis – across multiple dairy farms in Ukraine. Clinical examinations and therapeutic procedures were performed on more than 9,800 cows from 7 farms during 2022–2023. Diagnosis was conducted during scheduled functional hoof trimming using an orthopedic crush and was confirmed based on lesion morphology, presence of exudate, odor, and pain response. A locally formulated topical powder composed of copper sulfate, zinc oxide, iodoform, and potassium permanganate was applied to purulent–necrotic lesions and compared with a control treatment using a commercial chemi-spray. Therapeutic efficacy was evaluated by lesion healing dynamics and locomotion scores (AHDB system) on days 3, 14, 21, and 45. Digital dermatitis was identified as the predominant lesion, accounting for 50–66% of all diagnosed hoof disorders. The locally formulated powder showed markedly superior therapeutic performance relative to the chemi-spray, accelerating tissue repair and improving locomotion, with full recovery achieved in 86.7–100% of treated cows, depending on farm and lesion type. These findings confirm the persistently high prevalence of hoof diseases in dairy herds and demonstrate the clinical advantage of an affordable, locally prepared treatment formulation. Routine functional trimming, early lesion detection, and evidence-based local therapy remain critical components of effective lameness management in modern dairy production systems.
Discovery of New Antibacterial Peptide from Trypsin Hydrolysate of Monocled Cobra (Naja kaouthia) Venom Protein using C18 SPE Column and LC-HRMS Erlista, Garnies Putri; Raharjo, Slamet; Raharjo, Tri Joko
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.364

Abstract

Antimicrobial peptides (AMPs), as new antibiotic candidates, have the potential to treat infectious diseases. Peptides from N. kaouthia venom proteins can be used to produce AMP. The aim of this study was to identify the antibacterial potential of peptides derived from N. kaouthia venom protein purified by a reverse-phase solid-phase extraction column (SPE C18). N. kaouthia venom protein was isolated using Amicon® Ultra-15 with a 3000 Da centrifugal filter and then hydrolyzed using trypsin. The hydrolyzate was fractionated using reverse-phase (SPE C18), and the resulting fraction was tested for its antibacterial activity against S. aureus (Gram-positive) and E. coli (Gram-negative) bacteria. The most active fraction as an antibacterial was analyzed by high-resolution mass spectrometry (HRMS). The results showed that the 50% methanol fraction was active against E. coli bacteria, and the 100% methanol fraction was active against S. aureus. There are seven peptides identified, namely TVPVKR, TTMMNMLK, WWSDHR, SSLLVK, NSLLVK, LIPIASK, and GALMLK, which are responsible for the activity of the 50% methanol fraction. Meanwhile, there are two peptides, namely SSLLVK and NSLLVK, responsible for the activity of the 100% methanol fraction. LIPIASK and GALMLK peptides are predicted to show better potential as antimicrobial peptides.
Furosemide Increases GABAA Receptor Activity via Antagonism to Sodium-Potassium-Chloride Cotransporter 1 In Silico and In Vivo Siregar, Marsintauli Hasudungan; Nurdiana, Nurdiana; Bal’afif, Farhad; Djajalaksana, Susanthy; Setiawansyah, Arif
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.365

Abstract

GABAA receptor dysfunction and altered chloride homeostasis significantly contribute to seizure pathophysiology, with the sodium-potassium-chloride cotransporter 1 (NKCC1) playing a crucial role in regulating neuronal excitability. This study investigated furosemide's capacity to enhance GABAA receptor activity through NKCC1 antagonism and evaluated its therapeutic profile in combination with diazepam for seizure management. Comprehensive molecular docking analyses were conducted to assess binding affinities of furosemide and diazepam to NKCC1, followed by in vivo experiments using pentylenetetrazol-induced seizure models to evaluate GABAA receptor expression, seizure duration, and multiple pathophysiological biomarkers. Molecular analysis revealed that furosemide demonstrated measurable NKCC1 binding capacity (binding energy: -7.09 kcal/mol; Ki: 6.34 µM), though significantly lower affinity compared to diazepam (binding energy: -7.83 kcal/mol; Ki: 1.81 µM). The furosemide-diazepam combination exhibited complex competitive binding interactions, with furosemide substantially reducing diazepam's NKCC1 binding affinity. NKCC1 antagonism by furosemide effectively enhanced GABAA receptor expression by 29.8 ± 1.60% when used alone and 37.60 ± 2.0% in combination with diazepam. However, combination therapies resulted in significantly longer seizure durations (80 ± 3.0 s) compared to diazepam monotherapy (42.5 ± 2.10 s), suggesting antagonistic interactions on acute seizure suppression that may reflect altered chloride gradients or competitive pharmacokinetic effects. Despite reduced efficacy in seizure termination, combination therapy demonstrated selective advantages in other pathophysiological domains, including superior blood-brain barrier protection (reduced albumin level to 90.90 ± 2.70 µg/mL) and reduced excitotoxic damage. These findings indicate that furosemide-diazepam combination therapy presents a complex therapeutic profile characterized by trade-offs between acute seizure control and neuroprotective mechanisms. The data suggest potential utility in maintenance therapy or prevention of seizure-related complications rather than acute seizure termination, warranting further investigation into temporal optimization strategies and dose modifications.
Subsurface Identification Using Horizontal to Vertical Spectral Ratio and Inverse Velocity Modelling in Suoh Pull-apart Basin, Indonesia Mulyasari, Rahmi; Sarkowi, Muh; Haerudin, Nandi; Utama, Hari Wiki; Widiatama, Angga Jati; Darmawan, I Gede Boy; Kurniawan, Andri; Husni, Yayan Mi’rojul; Hesti, Hesti; Fatmawati, Nofita; Marlina, Serli
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.366

Abstract

Suoh is a pull-apart basin located along the Semangko segment of the Sumatra Fault Zone and filled by thick fluvio-volcanic and alluvial deposits that strongly amplify seismic waves and increase surface damage during earthquakes. This study aims to perform seismic micro-zonation of Suoh using the horizontal-to-vertical spectral ratio (HVSR) method combined with shallow shear-wave velocity (Vs) inversion to support hazard assessment and land-use planning in the Suoh aspiring geopark. Single station microtremor measurements were acquired at 30 sites and processed with Geopsy to obtain dominant frequency (f0), amplification factor (A0), and seismic vulnerability index (Kg). The HVSR curves were further inverted using the Dinver module to retrieve 1D Vs profiles, with initial models constrained by the global CRUST1.0 model and local geology. The results indicate strong lateral variability in site conditions, with low f0 and high A0 values concentrated in the central–western basin, producing high Kg and reflecting thick-soft sediments overlying deep engineering bedrock (Vs ≥ 800 m/s) at depths exceeding 80–100 m. These high-vulnerability zones spatially coincide with shallow seismicity associated with the Semangko Fault. Based on Kg, the study delineates low, moderate, and high seismic vulnerability zones, highlighting areas where seismic amplification and resonance effects are most pronounced. The combination of HVSR parameters with 2D Vs imaging provides a practical framework for identifying high-risk zones and guiding risk-informed land-use planning in tectono-volcanic basins such as Suoh.
Antioxidant and Anticancer Effects of Red Okra (Abelmoschus esculentus L.) Ethanol Extract through In Vitro and In Vivo Colorectal Cancer Models Wahyuningsih, Sri Puji Astuti; Mamuaya, Brigita Klara Krisdina; Dewi, Firli Rahmah Primula; Hapsari, Lukiteswari Dyah Tri; Kusuma, Baskara Wiku Adi; Nurhayati, Awik Puji Dyah
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.367

Abstract

Colorectal cancer (CRC) remains the second leading cause of cancer-related mortality worldwide. Affordable herbal sources such as red okra (Abelmoschus esculentus L.) pods have gained attention as potential alternative therapies for CRC. This study aimed to evaluate the antioxidant and anticancer effects of red okra ethanol extract (ROE) using both in vitro and in vivo colorectal cancer models. The antioxidant activity of ROE was assessed using the DPPH assay, while cytotoxic activity was evaluated using the MTT assay on SW480 and HCT116 cell lines. An in vivo study was conducted using rats divided into six groups: normal control, negative control (MNU 10 mg/kg BW), positive control (MNU + methotrexate 0.08 mg/kg BW), and treatment groups receiving MNU combined with ROE at doses of 50, 100, and 200 mg/kg BW for 28 days. Serum levels of Bcl-2, COX-2, VEGF, and MMP-9 were analyzed, and histopathological evaluations of colon tissues were performed. Data were statistically analyzed using one-way ANOVA followed by Duncan’s post hoc test. Statistical significance was determined at p<0.05. ROE exhibited potent antioxidant activity (IC₅₀ = 59.66 ppm) and induced cytotoxic effects by reducing SW480 cell growth and inhibiting HCT116 cell proliferation. Moreover, ROE significantly decreased the expression of Bcl-2, COX-2, VEGF, and MMP-9. These biomarkers are associated with apoptosis inhibition, angiogenesis, inflammation, and metastasis, respectively. Histopathological analysis confirming reduced inflammatory infiltration and suppression of colon carcinogenesis. The optimal in vivo dose was 50 mg/kg BW. These findings support the development of ROE as a promising natural agent for colorectal cancer prevention and therapy.
Modification of a Gene Cassette to Express Proglargine in Pichia pastoris: Elimination of Glu-Ala Repeats, Spacer and C-peptide Sequences Nasution, Uli Julia; Astuti, Rika Indri; Wahyudi, Aris Tri; Hardianto, Dudi; Martius, Efrida
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.368

Abstract

The growing global prevalence of diabetes mellitus has sharply increased the demand for insulin and its analogues. Pichia pastoris is a well-established system for recombinant pro-insulin and its analogues production. However, conventional gene constructs often include additional sequences, such as Glu-Ala repeats, spacer peptides, and c-peptides that complicate downstream processing and reduce efficiency. This study aimed to construct and express a proglargine (PG) gene cassette lacking the Glu-Ala repeats, spacer, and c-peptide in P. pastoris GS115 to obtain a uniform PG protein. The recombinant vector propagated in Escherichia coli TOP10F’, then expressed in P. pastorisGS115. Selected transformants were cultivated in YPG medium, then induced with 1% and 2% methanol daily in BMMY. The optimum methanol concentration further evaluated in ½ BSM induction medium. The result demonstrated that optimal PG expression was achieved with 2% methanol induction in BMMY, producing higher levels than those with ½ BSM. Among the transformants, PG.c2 produced the highest PG protein in BMMY medium induced with 2% methanol. Dot-blot analysis confirmed the secretion of PG, and LC-HRMS analysis demonstrated 100% amino acid sequence coverage of PG, confirming the integrity and completeness of the expressed protein. This study presented a newly modified PG gene cassette, inserted into pPICZαA vector, to express uniform secreted PG in P. pastoris GS115. By simplifying the precursor structure, a more homogeneous precursor product can be obtained, which is expected to simplify purification and also the downstream enzymatic process of PG into mature insulin glargine.
Correlation of Solar Activity and Interplanetary Parameters on Cosmic rays Intensity Investigated through Multivariate Analysis from 1964 to 2020 Putri, Annisa Novia Indra; Herdiwijaya, Dhani
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.369

Abstract

This study investigated the relationship between the most influential solar and interplanetary plasma parameters and cosmic ray modulation. A principal component analysis (PCA) method was used. The variations of the cosmic ray intensity were obtained at 6 different locations with different latitudes and cutoff rigidities. These stations were located at mid- to high latitudes (SOPO, OULU, and NEWK), while the remaining three were located at low latitudes (MXCO, TSMB, and PSNM). A parameter ranking based on PCA confirmed that sunspot number, interplanetary magnetic field (IMF) magnitude, heliospheric current sheet (HCS) tilt, halo coronal mass ejection (CME) velocity, Bz, and proton density have the highest link with the modulation of cosmic ray intensity. Meanwhile, the PCA analysis at low latitudes revealed that the cone angle and the magnetic field components By and Bx along the y- and x-axes, the mean magnetic field of the Sun, the HCS tilt, and the IMF magnitude exert the greatest correlation on the cosmic ray intensity modulation at this station. The correlation between the analyzed parameters and cosmic ray modulation appears to vary with latitude.
Modulation of AR-FGF-2-TGF beta Axis by Cosmos caudatus Suppresses Prostatic Epithelial Proliferation in a BPH Rat Model: A Comparative Study with Finasteride Sahara, Nita; Mudigdo, Ambar; Wasita, Brian; Pamungkasari, Eti Poncorini; Dirgahayu, Paramasari; Cilmiaty, Risya
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.370

Abstract

Benign prostatic hyperplasia (BPH) progression is critically influenced by the androgen receptor (AR), Fibroblast growth factor-2 (FGF-2), and transforming growth factor-β (TGF-β) signaling axis. Although finasteride (FIN) is the standard therapeutic agent, its use is limited by adverse effects. This study evaluated the efficacy of Cosmos caudatus (CC) extract, administered alone or in combination with FIN, in modulating the AR–FGF-2–TGF-β axis in a testosterone-induced BPH rat model. Rats were divided into five groups (n = 6): Normal (N), BPH control (KN), FIN (KP; 0.44 mg/kg), CC extract (P1; 250 mg/kg), and combination (P2; CC 250 mg/kg + FIN 0.44 mg/kg). Prostatic AR, FGF-2, and TGF-β expression was analyzed using immunohistochemistry (IHC) with H-score quantification. Both KP and P1 significantly reduced AR (KP: 112 ± 15; P1: 105 ± 12 vs KN: 195 ± 18, p < 0.05) and FGF-2 (KP: 98 ± 14; P1: 92 ± 11 vs KN: 180 ± 20, p < 0.05), approaching normal values (AR N: 90 ± 10; FGF-2 N: 85 ± 9). In contrast, combination therapy (P2) failed to suppress AR (188 ± 17, p = 0.936) and FGF-2 (175 ± 19, p = 0.870) and induced a significant increase in TGF-β (P2: 145 ± 16 vs KN: 110 ± 12, p < 0.05). All treatment groups showed reduced epithelial thickness and prostate index (p < 0.05). These findings indicate that CC extract alone exerts therapeutic effects comparable to FIN by modulating the AR–FGF-2 axis, whereas combining it with FIN disrupts pathway regulation and promotes a pro-fibrotic response. The study supports the potential of CC extract as an effective alternative for BPH management without pharmacologic complications.
Hierarchical NiO/TiO2-Biomass-Derived Porous Carbon Composite with Improved Electrochemical Kinetics for Energy Storage Applications Nurdin, Muhammad; Maulidiyah, Maulidiyah; Watoni, Abdul Haris; Zakir, Muhammad; Muliadi, Muliadi; Muzakkar, Muhammad Zakir; Azmi, Khusnul Ulul; Edihar, Muh; Zulfan, Ahmad
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.371

Abstract

The development of high-performance and sustainable anode materials remains a crucial challenge for next-generation energy storage systems. In this study, NiO/TiO₂–biomass-derived porous carbon (BPC) composites were successfully synthesized using palm kernel shell-derived porous carbon as a conductive matrix. Structural and morphological characterizations confirmed the homogeneous distribution of NiO and TiO₂ crystals within the porous carbon framework. The FTIR and XRD analyses verified the presence of metal-oxygen bonds and high phase purity, while SEM revealed a well-developed porous structure. Electrochemical evaluations were performed in KOH solution using a three-electrode configuration. The optimized NiO-TiO2/BPC (1:2) electrode delivered a specific capacity of 7.76 mAh g⁻¹ at 10 mV s⁻¹ and sustained 3.59 mAh g⁻¹ at 80 mV s⁻¹, demonstrating favorable rate capability. Galvanostatic charge-discharge measurements further confirmed the improved performance, reaching 8.75 mAh g⁻¹ at 3 A g⁻¹. This enhanced electrochemical behavior is attributed to the synergistic integration of conductive porous carbon with electroactive NiO and TiO₂ phases. These findings indicate that the NiO-TiO₂/BPC composite functions as a hybrid alkaline electrode material and has potential for safe and sustainable aquatic energy storage devices.

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