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Berkala Penelitian Hayati

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Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur

ISSN : 08526834 EISSN : 2337389X DOI : https://doi.org/10.23869/bphjbr

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 507 Documents

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VEGETATION SPECIES ABUNDANCE IN MANGROVE ECOSYSTEM OF PASIR MENDIT AT BOGOWONTO LAGOON KULON PROGO YOGYAKARTA Maizer Said Nahdi; Ardyan Pramudya Kurniawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013
Publisher : The East Java Biological Society

This research was conducted on Pasir Mendit, Bogowonto Lagoon. Pasir Mendit, Bogowonto Lagoon is one of unique beaches in Indonesia, with sand du-nes that protecting the beach from Indian Ocean €™s pounding waves. The purpose of this research is to shows the relation between the environmental fac-tors and the abundance of vegetations in the mangrove €™s ecosystem. Quadrates method were used for collecting the data, by making several plots of the study of growth forms. Connonical Correspondence Analysis (CCA) were used to analyze the correspondential relationship between vegetation and the environmental factors. The result of this research shows that there are 19 species of mangroves and its associates and unity. All of the locations are colo-nized by Acanthus ilicifolius bushes and Derris heterophylla woody climber, which is the r-strategy, with high level of density. Sonneratia alba and Rhi-zophora mucronata is the dominating species in all locations with varied abundance and importance value. This would be linked with the relatively high concentration of C organic, NO3 and SO4. The presence of Acanthus ilifolius and Derris heterophylla shows that the mangrove ecosystem of Men-dit has suffered from destructions. With the existence of mangrove in Bogowonto Lagoon, the place is suggested to be a conservation location model for other lagoons in Yogyakarta and Central Java.

DIVERSITY OF BAMBOOS AROUND SPRINGS IN MALANG EAST JAVA Solikin Solikin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013
Publisher : The East Java Biological Society

Bamboos have important roles to people in the villages area. They are planted and used by the people for making houses, food, buckets, fences, ropes, fuels, musical instruments and plaits. The root distribution of Bamboos is large and fibrous, also the growth of their new clumps is ascendant so the Bamboos has good potency for water and soil conservation on river banks, around the springs, hillsides and scarps. The survey to invent the bamboos growing around the springs was conducted in Singosari, Lawang, Karangploso, Dau and Lowokwaru Malang East Java in May 2009. The Bamboos invented at 0-100 m from the springs. The results Showed that there were four Bamboos founded around the springs namely Bambusa blumeana,Dendrocalamus asper, Gigantochloa atter and Gigantochloa apus. Dendrocalamus asper was the most dominant species founded around the springs with relative frequency, relative density and important value index is 45.83 ; 58.49 and 104.32 respectively.

CORAL REEFS DIVERSITY IN GILI GENTING ISLAND SUMENEP MADURA Fathorrohman Fathorrohman; Romaidi Romaidi; Bayyinatul Muchtaromah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013
Publisher : The East Java Biological Society

Coral reef ecosystem is the most threatened ecosystem among marine ecosystem in the world due to the combination of anthropogenic and natural disturbances. More research is needed to be monitor ed and assess coral reef ecosystems, which will be used to fin d understanding of the ecological integrity and further improvement of the protection strategy in the future. This re search was aimed to know the diversity of coral reef diversity at Gili Genting Island Sumenep Madura and evaluate the condition of coral reef ecosystem based on cover the percentage. Line intercept method was used to understand coral reef diversity and its condition in the desired observation station. The result revealed that 9 families, 22 genera and 4 5 species of coral have been successfully found, in which Acropora is the most common genus found in in Gili Genting Island . The cover percentage analysis also indicated that the condition of coral reef ecosystem in Gili Genting Island could be classified to very bad category where th e total average of cover percentage is 12.55%. These findings provide the prelim inary information about the condition of coral reef ecosystem in small i sland that might be useful for the future integrated management based on ecological perspective.

EFFECT OF CALCUSOL TO REDUCE THE CALCIUM CRYSTAL RETENTION IN KIDNEY EPITHELIAL CELLS MODEL OF NEPHROLITHIASIS Ahmad Soni; Moch. Sasmito Djati; Sri Widyarti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013
Publisher : The East Java Biological Society

Kidney stones is a disease that characterized by a disturbance in the bladder. The main constituent of kidney stones namely Calcium Oxalate Monohydrate (COM) crystals. The presence of a COM crystal adhesion to renal tubular cells, will initiate the internalization which will further lead to the formation of crystals retention in the kidney. In Indonesia, there are many herbal products are considered able to cope the complaints due to the kidney stone disease. One of the herbal product is Calcusol „¢, which is the main constituent of those herbal product was the leaf extract of tempuyung. This study observed the effectiveness of Calcusol „¢ in reducing crystals retention that was formed in kidney epithelial cells model of nephrolithiasis. The result showed that Calcusol „¢ is able to reduce the average number of calcium crystals retention in the renal epithelial cells. It indicate that Calcusol „¢ has the ability to reduce crystals retention that already formed in renal epithelial cells. Furthermore, the results of this study are expected to be one of the considerations for further research on the potential of overcoming Calcusol „¢ in kidney stone disease

HUMAN SPERM PROTEIN 116 KDA: A CANDIDATE ANTIGEN FOR IMMUNOCONTRACEPTION TECHNOLOGY Umi Lestari; Aulanni’am Aulanni’am; Basuki B. Purnomo; Sutiman Bambang Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Spermatozoa membrane antigenic protein utilized as the substance candidate of immunocontraception has important criteria, that are only expressed in spermatozoa, cannot be found in the other tissue, and also should not have kinase activities. The observations done finally conclude 116kD, a sperm head’s protein is within the above criteria. It’s antigenic characters are shown with ability of it’s polyclonal antibody to bind the human spermatozoa, and interestingly this polyclonal antibody enable to block fertilization of other species in an in-vitro test experiments. This reseach also acquired that 116 kDa protein is specific and exists only in spermatozoa, and not in the other tissue such as spleen, kidney, pancreas, and epidydimic. This 116 kDa protein distribute along the whole area of the sperm, but mostly accumulated around the head up to the the neck. The imunohistological staining of the testis also indicate that this protein exists in the spermatid of the testes, but not be found in other somatic tissues, and it’s antibody is evidently was recognised by the goat and cow spermatozoa membrane protein resulted in blocking the fertilization of both species respectively. And the conclussion, 116 kD is Non-kinase protein specific only exists in the area of the head of human spermatozoa. It is therefore believed to be adequate candidate for antigen substance of immunocontraception technology.

GENETIC DIVERSITY OF Lactobacillus spp. OF NATURAL ETHAWAH GOAT MILK-FERMENTED WAS DETERMINED BY USING 16SRDNA WITH DDGE ANALYSIS Nur Kusmiyati; Nur Hidayat; Fatchiyah Fatchiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

phoresis (DGGE) analysis. Goat and bovine (as negative control) milk naturally fermented among 24hour until 6 days. Morphological and biochemistry of bacteria were characterized by standard methods. The total DNA of bacteria were isolated using alkali lysis, PCR amplification was carried out using 3 pairs of specific primers, DNA-amplified using DGGE and then to determine the relationship among Lactobacteria using NTsys package software V2.0. Phenotypical and biochemical study showed that the 11 strains are belonging to genus Lactobacillus. The dendogram results show all of isolates had similarity characters with genus Lactobacillus around 56-76%. According to morphological and DDGE profiles, we were identified that bacteria isolate of goat milk-fermented are K1A, and K3A are L. casei, and bacteria isolate K3B is L. plantarum.

ISOLATION OF Pichia manshurica PROTOPLAST FROM Dahlia sp. PLANT Wijanarka Wijanarka; Endang Sutariningsih Soetarto; Kumala Dewi; Ari Indrianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Isolation of protoplasts is an important step in the fusion process. Protoplasts are cells that have eliminated the cell wall, but the cell membranes and organs can still function properly. Pichia manshurica is one of indogenous yeast that derived from Dahlia’s plants. The success rate protoplast isolation was determined by various factors, include the age of the culture and the used of lytic enzymes. The purpose of this research is to get the perfect age of yeast culture that is ready to be harvested and also to get the appropriate concentration of Glucanex lytic enzymes which used for protoplast isolation. The yeast of Pichia manshurica grown on YPD broth medium and growth observed in turbidimetry. Observation of the growth of yeasts performed every 6 hours for 42 hours. Glucanex lytic enzyme concentration used for the isolation of protoplasts is 0 mg / mL (L0 = control), 2 mg / mL (L2) and 4 mg / mL (L4). The results showed that the age of the culture is right and ready for harvest at the age of 24 hours and Glucanex lytic enzyme concentration of 4 mg / mL (L4) is able to produce the best of protoplasts at 7.2 x 1010.

PATCHOULI ALKOHOL ISOMERS POGOSTEMON HERBA PREDICTED VIRTUALLY Sentot Joko Raharjo; Chanif Mahdi; Nurdiana Nurdiana; Wolfgang Nellen; Fatchiyah Fatchiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

The aim of our research is predicting the alpha-patchouli alcohol isomer Pogostemon Herba as inhibitors cyclooxygenase (COX-1 and COX-2) isoenzymes. The data for the alpha-patchouli alcohol isomer (CD521903, CD442384, and/or CD6432585) Pogostemon Herba were explored from the pubchem database. Molecular interaction studies with COX-1 and COX-2 from mouse were done using the molecular docking tools Hex 6.12 and LeadIT2 Bisolve. The analysis of the alpha-patchouli alcohol compounds of patchouli oil showed that alpha-Patchouli alcohol (CD521903) binds to COX-1 at active sites including: LEU223B, ASP228B, LEU237B, ARG 332B, TRP 138A, GLU 139A, SER 142A, ASN 143A, and the interaction to COX-2 at active site including: GLN 289B, GLU 290B, ARG 222B, LYS 211B, THR 212B, HIS 214B, ASN 382B, HEM682B, GLN 454B, HIS 386B, TRP 387B, HIS 388B, VAL 274B, GLN 203B, VAL 291B, VAL 295B. The interaction hydrogen bond energy between alpha-patchouli alcohol: (CD521903-COX-1 complexes (-4 kJ/mol) and CD521903-COX-2 complexes (-8 kJ/ mol) by LeadIT2 Biosolve. This suggests alpha-patchouli alcohol CD521903 as candidate for a selective COX-2 inhibitor. These in silico data need further analyses of biological function activity.

CONTAMINATION OF Pb, Cd, Cu, Zn IN BLACK POMFRET (Formio niger) AND TONGUE SAND (Cynoglossus lingua) FISH CAUGHT FROM THE WESTERN PART OF MADURA STRAIT AND ITS SAFE LIMITS CONSUMPTION Acivrida Mega Charisma; Bambang Irawan; Agoes Soegianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Contamination of Pb, Cd, Cu, Zn in the flesh on the two types of fish consisting pelagic fish include Black pomfert (Formio niger) whereas among other demersal fish include Tongue sand (Cynoglossus lingua), was conducted in March-December 2013, which was taken from the western part of Madura Strait (Gresik-Ujung pangkah, Surabaya-Kenjeran, and Sidoarjo-Sedati). Pb, Cd, Cu, and Zn detected using AAS (Atomic Absorption Spectrometry) brand Shimadzu AA-6200. The highest content of heavy metals, namely Pb in Cynoglossus lingua from the Sedati (5.63ppm±0.05), while a low of Cd in Formio niger from the Ujung pangkah (0.20ppm±0.004). The results show that there is a correlation between heavy metal with fish but not significantly. The content of Pb, Cd, Cu, Zn in 2 fish species originating from all locations stated below the exposure limits for consumption. Safe limit fish consumption of Formio niger is based on the WHO PTWI of 347.43 grams / week and Cynoglossus lingua 191.25 grams / week.

THE EFFECT OF BIOFERTILIZER FUNGI ON CIHERANG RICE GROWTH AT SOME LEVEL OF SOIL SALINITY YB. Subowo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

research about the effect of fungus contained biofertilizer on Ciherang rice that was growth on different level of soil salinity was conducted. One of the effect of global climate changes is the increase of sea water level. It leads to the expansion of sea water submerged land for agriculture. Salt intrution to the agriculture area considerably decrease soil fertility because of the high salinity. Some of microbes especially soil fungi such as Aspergillus sp and Penicillium sp. are able to grow at high salinity environment. Those fungi were also able to degrade lignocellulose, sollubilize in organic phosphate and provide organic phosphat and produce plant growth hormon especially IAA. Such activities benefit to improve soil fertility in high salinity land as a bio-fertilizer.The objective of this research was to know the growth of rice plant that treated with fungus contained bio-fertilizer on land with different level of salinity. The rice were planted in Green house of Cibinong Science Centre, Cibinong.The research was set up as complete random design with five replication. The rice were watered by 5 conditions: 50% of sea water, 100% of sea water, 100% sea water + 2 % NaCl , fresh water + 5 % NaCl and 100% fresh water as the control. Fertilizer was added to the medium twice. Ten grams of fertilizer were used per polybag (10g/7 Kg), 2 weeks after planting and before flowering subsequently. The observed parameters were plant height, number of tiller, leaves colour, biomass dry weight, soil organic carbon content, cellulosic and lignin degrading activities of the fungus, fungus phosphate-solubilizing potency and fungus production of IAA.The watering treatment lead to 5 level of salinity i.e. : 5,93 dS/m (50% sea water), 9,15 dS/m (100% sea water), 10,42 dS/m (sea water + 2% NaCl), 12,43 dS/m (fresh water + 5% NaCl) and 0,74 dS/m (fresh water). The result showed that among those 5 watering condition, the rice grew best on 5,93 dS/m (watering 50% of sea water). This result was considered as the best since the plant height and number of tiller were not significantly different with those of the control. Plants height and number of tiller were 74,4 cm and 11 respectively. On the higher salinity level the plant growth was inhibited. The ability of rice to withstand the high salinity possibly was supported by the fungus activities of providing organic phosphate and IAA growth substance.

Page 11 of 51 | Total Record : 507

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Sutiman Bambang Sumitro

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berkalahayati@yahoo.com

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Sutiman Bambang Sumitro

Contact Email berkalahayati@yahoo.com

Phone +62341570631

Journal Mail Official wulidanisa@berkalahayati.org

Editorial Address Jalan Surakarta No. 5 Malang, Indonesia

Location Unknown, Unknown INDONESIA

Abstract

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Contact Name
Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

Phone
+62341570631

Journal Mail Official
wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

Location
Unknown,

Unknown

INDONESIA