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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 507 Documents
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EFEK 2-METHOXYETHANOL TERHADAP STRUKTUR HISTOLOGI TESTIS MENCIT (Mus musculus) Alifah Hayati; Binti Yunaida; I.B. Rai Pidada; Win Darmanto; Dwi Winarni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/371

Abstract

This research has done to investigate the effect of 2-Methoxyethanol on the testicular histology of the male mice and also the influence the length of time after administration 2-ME stopped in the recovery of the spermatogenic cells and the diameter also the thicknes of seminiferous tubule. Thirty BALB/C male mice 8–9 week old, weighed 28–30 grams body weight. Those mice separated to 6 groups with 5 male mice each group. Those mice were treated with 2-ME 200 mg/kg body weight daily by intra peritoneal injection, within 3 weeks (K1). To investigate the influence the length of time after administration 2-ME stopped, the male mice after treated by 2-ME in 3 weeks also given by the length of time after 2-ME administration stopped 1, 2, 3 and 4 weeks (P1, P2, P3 and P4). The control animal given by intraperitoneal administration of saline. Histological observation was performed on the number of spermatogonium, primary spermatocyte, oval spermatid and the diameter also epithelial thickness of seminiferous tubules. The data were analyzed by One-Sample T-test to investigate the differences between K0 and K1. One Way ANOVA to investigate the influence the length of time after 2-ME administration stopped in the P1, P2, P3 and P4 and then continuing by LSD (Least Significant Difference) to show the differences groups of treatment. The result showed that administration 2-ME could destroy the seminiferous tubules in the testes. Its presented by the decreasing of the number spermatogonium, primary spermatocyte, oval spermatid and diameter also epithelial thickness of seminiferous tubule. The length of time after administration 2-ME stopped could recover seminiferous tubules condition. Its presented by the increasing of the number spermatogonium, primary spermatocyte, oval spermatid, and diameter also epithelial tickness of seminiferous tubules. The conclution of this research were, 2-ME could destroy the testicular histology of the male mice and the length of time after administration 2-ME stopped have linear correlation with seminiferous tubules recovery.
KAJIAN PENDAHULUAN UJI TOKSISITAS EKSTRAK AIR MISELIA DAN TUBUH BUAH JAMUR SHIITAKE (Lentinus edodes) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST) Noor Erma NS; Tri Sundari; Arie Ika Susanty; Dwi Riani Oktavia Palupi; Isnaeni; Sukardiman
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/372

Abstract

Shiitake mushroom (Lentinus edodes) is one of the wood mushroom types that can be consumed as a food as well as for a medical purpose. Lentinan, a polysaccharide contained in shiitake, is well known for its use on cancer medication. Mycelium of Shiitake mushroom contains lentinan the same as other part of the mushroom like fruity body. Toxicity of the lentinan in mycelium compare to the fruity body has been first conducted by using Brine Shrimp Lethality Test (BST). Using Potato Dextrose Broth media with the growth rate of 3.88% did mycelium multiplications. Probit analysis showed that the toxicity of the mushroom’s cap, stem, and mycelium of Shiitake mushrooms is LC50 = 648.76507 mg/ml LC50 = 489.39444 mg/ml, and LC50 = 481.16941 mg/ml respectively.
BIOAKUMULASI LOGAM BERAT Cu OLEH Bacillus sp Riesta Primaharinastiti; A. Toto Poernomo; Noor Erma S.
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/373

Abstract

The research was conducted to investigate the ability of Bacillus sp in accumulating Cu and how much it can be acumulated. The medium used to growth the bacterium was Nutrient Broth and Atomic Absorption Spectrophotometry methods was used to assay the Cu, both in the cells and medium. The result of this study showed that Bacillus sp incubated in the Nutrient Broth medium containing 10 ppm of Cu, with continuous stirring in the room temperature was able to reduce Cu in the medium 8.912–12.623% and accumulate Cu in the cell 0.1149–0.1400 %/mg cells. Based on this result, it is necessary to develop more studies to find out what factors that influence the accumulation process and to optimize the bioprocess.
BIOTRANSFORMASI ADIPONITRIL OLEH Bacillus licheniformis BA2 Ahmad Thontowi; Eko W. Pamuji; Bambang Sunarko
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/374

Abstract

Adipic acid represents one of the especial materials which used for the synthesis of nylon 6,6,- is a very important material results from polyamide industry. Adiponitrile biotransformation become adipic acid represent an alternative synthesis besides chemically. The purpose of this research was to determine optimum conditions for Bacillus licheniformis BA2 growth for adiponitrile degradation, and also know its pattern. The obtained information, to be expected can be used as reference for scaling up of adipic acid production. B. licheniformis BA2 was able to utilize acetonitrile and adiponitrile as the sole source of carbon and nitrogen. The growth on adiponitrile 120 mM mixture with acetonitrile 30 mM gave higher growth rate and biomass yield than growth on another subsrates. B. licheniformis BA2 have lag phase during 68 hours, logaritmic phase passed by during 104 hours, while stasioner phase just reached by after 172 hours. High-performance liquid chromatography of adiponitrile degradation by crude bacterial revealed a decrease in adiponitrile with the sequential formation of adipamide and adipic acid. Ammonia was also detected by colorimetric procedures. As for adipic acid rendemen at 420 minutes equal to 19.35%.
CHARACTERISTICS OF THE PEAT SOIL Bacilllus sp. Iman Hidayat
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/375

Abstract

Twenty two strains of Bacillus species have been isolated from the peat soil of Tanjung Puting, Central Kalimantan. All strains devided into seventeen groups which characterized using morphology and biochemical tests. All strains have been tested for lipase, amylase, protease, cellulase, and phosphatase activity. Thirteen Bacillus isolates tested gave positif reaction for lipase activity. Bacillus AR 018 was the best isolate for amylase activity, Bacillus AR 001 and Bacillus AR 002 for protease activity, Bacillus AR 009 for cellulase activity. Only one isolate shows positive reaction for phosphatase activity, notably Bacillus AR 007.
IDENTIFIKASI BERAT, DIAMETER, DAN TEBAL DAGING BUAH MELON (Cucumis melo, L.) KULTIVAR ACTION 434 TETRAPLOID AKIBAT PERLAKUAN KOLKISIN Y. Ulung Anggraito
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/376

Abstract

Indonesian farmers are very dependence on certificated seed from another countries. In the other side the natural resources and men powers very abundance. For these reason it is properly developed the research in agriculture sector, especially on plants breeding. It can be hoped that in the future the dependence on certificated seed from another countries can be minimized. The objective of this research were: (1) to find out the concentration and dipping period which is effective to induce polyploid in musk melon plant, (2) identify the weight, diameter, dan flesh thickness of tetraploid musk melon as result of colchicines treatment. The sample of this research was Action 434 musk melon cultivar, product of Chia-Thai Seed, Thailand. The number of sample was 480 plants, which plants on field randomly. There were four colchicines concentration as an independent variable: 0.0%, 0.05%, 0.10% and 0.2%. The dipping period were 12, 16, 20, and 24 hours for each concentration respectively. Completely Random Design was used in three replications. Data measurement were analyzed with Two Way ANOVA, DMRT, and LSD. From this research can be concluded that: (1) 0.2 % colchicine is the most effective concentration to induce polyploid on musk melon, with dipping period effective varied from 16–24 hours, (2) there are changes in weight, diameter, and flesh thickness characters, with the increased tendency of each character in definite norm.
8-HIDROKSI-DEOKSIGUANOSIN SEBAGAI SALAH SATU INDIKATOR INFERTILITAS PRIA Sudjarwo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/377

Abstract

DNA in human’s sperm is located in nucleus (nDNA) and mitochondria (mtDNA). DNA oxidation is indicated from high concentration of 8-OH-dG. It is very important to identify factors that can cause genetic dysfunction risk responsible for genetic changes. 8-OH-dG compound are a promutagenic of DNA dysfunction of deoxyguanosine oxidation by oxygen radical. ROS is an oxidation, which in high concentration can oxidize DNA. Guanine is a nucleotide, which prone to oxidation, with 8-OH-dG as the end product. Detection of 8-OH-dG can be done by HPLC method. These results indicated that there were significant differences (p < 0.01) in detection of 8-OH-dG in sperm, between normospermia with asthenospermia and normospermia with oligospermia. Correlation (r) of 8-OH-dG with motile spermatozoa is – 0.7111. Immotile spermatozoa caused by oxidation or DNA damage are shown by high concentration of 8-OH-dG. In normal physical condition, sperm produces oxidant. High oxidant is toxic to sperm, which influence their motility. Sperm’s motility is one of factors causing male infertility.
KEKAYAAN JENIS PORTUNIDAE DI SISI SHIPPING LINE SELAT MADURA Bambang Irawan; Agoes Soegianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/378

Abstract

A monitoring activity for the existence of portunid crabs in the narrowest part of Madura Strait has been done from 2001 to 2004. Crabs have been collected in June or July each year in day time during spring tide. Crab collection was carried out in four sampling station (I to IV) using 0,5 cm trawl net for 10 minutes netting in each time. The results show that during four year monitoring six species of portunid crab has been captured. Among the six species three of them, Charybdis affinis, C. anisodon, and Portunus pelagicus were collected each year even with difference level in local distribution. C. affinis and P. pelagicus have been monitored in the same three stations, and C. anisodon has also been monitored in three stations but differs from other three portunid crab species, C. annulata. P. trituberculatus, and Scylla paramamosain were very rare and have been captured in less then three sampling times collection. We conclude that in this area the portunid crab has been dominated by C. affinis which have less economic value, and for C. anisodon even the number higher than other portunid genus the occurrence is not stabil as in C. affinis.
KEBERADAAN TELUR CACING PARASIT PADA SISWA SD DI SEKITAR INSTALASI PENGOLAHAN AIR LIMBAH (IPAL) TERPADU KOTA MALANG DAN HUBUNGANNYA DENGAN KEPADATAN TELUR CACING PADA AIR LIMBAH PERUMAHAN DI IPAL TERPADU Sofia Ery Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/379

Abstract

The research to find out the existence, kinds, and prevalence of parasitic worm egg on the hands and nails of elementary school students lives in IPAL area also its existence in the water of housing dump has been conducted recently. It also analyze the correlation between the number of parasitic worm egg population on the elementary school students hands and nails lives in IPAL area and the water of housing dump in IPAL Terpadu Kota Malang. The research used descriptive explorative method and conducted on June–August 2004. The sample of parasitic worm egg is taken from elementary school students hands and nails ilves in IPAL area and the water of housing dump in IPAL Terpadu Kota Malang. The elementary shools taken as samples area SDN Tlogomas 1, SDN Bareng 3, SDN Mulyorejo 1, SDN Mergosono 1, and SDN Mergosono 2. The locations of IPAL Terpadu Kota Malang used in this research are Tlogomas, Bareng, Mulyorejo, Mergosono Gang 1, and Mergosono Gang 3 area. Identification of worm egg is conducted in Biology Departement, Faculty of Mathematics and Science, State University of Malang. The data taken are in the form of kinds of parasitic worm egg based on morphology characteristics and it will be analyzed descriptively. Then the prevalence of each kind of parasitic worm egg will bw counted. Then the researchers do some correlation analysis. Findings of this research show that from 5 samples of elementary school used in this research only the hands and nails of students in SDN Mulyorejo 1 have no human parasitic worm egg while in four others found 4 kinds of human parasitic Nematoda they are Ascaris lumbricoides, Enterobius vermicularis, Trichuris trichiura, and Ancylostoma duodenale. From 5 areas of IPAL Terpadu Kota Malang used in this research only in Bareng area it is not found while in four others found 3 kinds of worm egg they are A. lumbricoides, E. vermicularis, and T. trichiura. Egg prevalence of each kind of parasitic worm species on the elementary shool students lives in IPAL Kota Malang area are A. lumbricoides is 65.22%, E. vermicularis is 21.47%, T. trichiura is 11.59%, and A. duodenale is 1.45%. Egg prevalence os each parasitic worm species in IPAL Terpadu Kota Malang are A. lumbricoides is 88.40%, E.vermicularis is 10.15%, T. trichiura is 1.45%, and A. duodenale is 0%. In general is a relationship between the number of parasitic worm egg on the hans and nails of elementary school students lives in IPAL area and worm egg in the water of housing dump in IPAL Terpadu Kota Malang.
TINGKAT PLOIDI DAN TIPE REPRODUKSI Dryopteris sparsa DI HUTAN WISATA CANGAR KOTATIF BATU JAWA TIMUR Siti Zubaidah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/380

Abstract

Study of ploidy level and reproduction type of the fern species Dryopteris sparsa growing in Hutan Wisata Cangar (HWC) Kotatif Batu Jawa Timur and their relationship to the variation of leaf form and size was carried out. Ploidy level was determined based on somatic chromosome number counts by using standard squash methods. Reproduction type was examined by a spore count methods. The morphological characters that examined were the blade length, ratio of basal pinna length to blade length, ratio of stipe length to leaf length and ratio of basal basiscopic pinnule length to basal pinna length. We found that D. sparsa have two forms of ploidy levelreproduction type, there were sexual diploid (2n = 58) and sexual tetraploid (2n = 164). They could be differentiated by the variation of leaf form and size.

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