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All Journal Pharmaciana: Jurnal Kefarmasian UNEJ e-Proceeding JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Science and Technology Indonesia Majalah Farmasetika Berkala Penelitian Hayati
Riesta Primaharinastiti
Departemen Ilmu Kefarmasian, Fakultas Farmasi, Universitas Airlangga, Jawa Timur, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Other

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Pengaruh Proses Fermentasi pada Daun Centella asiatica oleh Acetobacter tropicalis Terhadap Aktivitas Trombolitik Lailatul Nuraini; Bambang Tri Purwanto; Achmad Syahrani; Riesta Primaharinastiti; Achmad Toto Poernomo
Majalah Farmasetika Vol. 6, Supl. 1, Tahun 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/mfarmasetika.v6i0.36665

Abstract

Agen trombolitik merupakan plasminogen activator yang dapat memecah fibrin menjadi fibrin degradation product (FDP) dan dapat digunakan pada terapi penyakit kardiovaskular. Agen trombolitik dapat diperoleh dari mikroorganisme seperti Acetobacter tropicalis InaCC B374 dan dari tanaman seperti Centella asiatica. Kedua sumber agen trombolitik tersebut dapat dilakukan kombinasi melalui proses fermentasi untuk meningkatkan efek terapetiknya. Proses fermentasi sendiri dipengaruhi oleh beberapa faktor termasuk media fermentasi dan waktu fermentasi. Penelitian ini bertujuan untuk mengetahui pengaruh proses fermentasi terhadap peningkatan aktivitas trombolitik dari hasil fermentasi Centella asiatica oleh Acetobacter tropicalis InaCC B374 pada berbagai variasi waktu fermentasi. Preparasi dilakukan dengan memfermentasi Centella asiatica selama 24, 48, dan 72 jam pada suhu 30°±1°C dengan kecepatan pengocokan 100 rpm kemudian ditentukan aktivitas trombolitiknya dengan metode clot lysis yang dilakukan inkubasi pada suhu 37°±1°C selama 60 menit. Hasil pengujian aktivitas trombolitik menunjukkan bahwa terjadi peningkatan aktivitas trombolitik setelah dilakukan proses fermentasi selama 24, 48 dan 72 jam dan aktivitas trombolitik maksimum tercapai pada hasil fermentasi 72 jam. Centella asiatica yang difermentasi selama 72 jam menunjukkan nilai indeks trombolitik yang paling besar (82,03) jika dibandingkan dengan infusa Centella asiatica tanpa fermentasi (37,39) dan Acetobacter tropicalis InaCC B374 (37,68). Disimpulkan bahwa proses fermentasi Centella asiatica oleh Acetobacter tropicalis InaCC B374 secara signifikan dapat meningkatkan aktivitas trombolitik keduanya
Determination and stability testing method of chlorpheniramine maleate in the presence of tartrazine using HPLC Asri Darmawati; Febri Annuryanti; Riesta Primaharinastiti; Isnaeni Isnaeni
Pharmaciana Vol 10, No 3 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (590.763 KB) | DOI: 10.12928/pharmaciana.v10i3.17409

Abstract

The single-component CPM tablet mostly used sodium tartrazine as the yellow coloring agent. Sodium tartrazine is soluble in solvents used to extract CPM from tablet and suspected interference CPM determination especially after forced degradation for stability indication testing of CPM tablets. This study aimed to develop a selective, accurate and precise method for determination and stability testing of chlorpheniramine maleate (CPM) in the presence of tartrazine in the tablet. A µBondapak® C18 column (3.9 x 300 mm, 10 µm) with diode array detector was used for separation. The mobile phase was a mixture of methanol and 0.2% triethylamine (90:10) with a flow rate of 2 mL/minutes. The validated HPLC method was used for CPM determination in tablet samples that had been forced degraded using dry heat at 105oC, UV radiation of 254 nm, hydrolysis with 1N NaOH, 1N HCl and oxidation using 5% H2O2. The HPLC chromatogram showed that CPM split into chlorpheniramine (CP) and maleic acid (MA). Resolution (Rs) among CP and the other analytes especially with the products resulting from the forced degradation by heat, UV radiation, HCl, and H2O2 were good. The CPM hydrolysis using NaOH caused the CP not completely separated from the degradation product due to tailing or overlapping peaks. The proposed HPLC method was valid for the determination of CPM in tablets containing tartrazine. Even though the stability-indicating method was inadequate especially for the result of the CPM hydrolysis process using NaOH. 
THREE-WAVELENGTH SPECTROPHOTOMETRIC METHOD VALIDATION FOR DETERMINATION OF PREDNISONE TABLET CONTAINING COLORING DYES Riesta Primaharinastiti; Ika Rizki Helwandi
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Asthma is a chronic inflammatory disease that ranksfifth out of the top ten causes of morbidity1. Thedrug therapy commonly used for asthma iscorticosteroid, for example is prednisone2. Thedetermination of the drug in the pharmaceuticaldosage form is very important to ensure its safetyand efficacy. Assay method of prednisone in tabletaccording to Farmakope Indonesia is using HighPerformance Liquid Chromatography (HPLC)3, whichhas good accuracy and precision, but high cost andtook a longer analysis time. These aspects aremaking it less suitable for quality control on a regularbasis. UV-Vis spectrophotometry method could beused as the alternative assay method to determineprednisone in tablet, as prednisone haschromophore and auxochrome groups4. UV-Visspectrophotometry method has shorter analysistimes and lower operating costs while still providinga high precision5. Spectrophotometric method UVVishas been used in previous studies of prednisoneand proven to give good results, for example in thedissolution test6,7. Singh and Verma8 conductedresearch to determine prednisone in tablets basedon the color occurred after prednisone reacted withFeCl3 and K4Fe(CN)6, forming a blueish greensolution. The solution then measured byspectrophotometer giving the analytical wavelengthat 780 nm.The addition of coloring agents in pharmaceuticalpreparations intended to identify products inproduction and distribution, distinguishing the samedose of the drug, preventing product counterfeitingand stability indicators9. However, coloring agent ordye in the formulation could affect the analysisprocess, such as background interfering in theanalytical instrument measurement. In Indonesia,usually we can find prednisone tablet in green color.Dyes used in this study is a mix of light green andtartrazine resulting in a light green color. These dyesalso have a cluster of chromophore and auxochrometo provide absorbance in the UV-Vis spectrumregion. Observing the prednisone solution containing0.1% light green and tartrazine solution at themaximum wavelength of prednisone (238 nm) willgive absorbance of the solution 5% higher.To overcome this problem, the analysis of thespectrophotometry is applied by multi-wavelengthmethod. Dual- or three-wavelengthspectrophotometry methods are very useful toeliminate the absorbance influence of interferingcomponents.a.b.c.Figure 1. Chemical Structure of : a. Prednisone, b. Lightgreen dye, c. TartrazineThe aim of this research was validating threewavelengthspectrophotometry method todetermine prednisone in tablet, containing coloringdyes. The method validation parameters defined inthis research are the
Optimasi Metode dan Uji Stabilitas pada Penetapan Kadar Filantin dalam Ekstrak Phyllanthus niruri Menggunakan KLT-Densitometri Rachma Nurhayati; Riesta Primaharinastiti; Mochamad Yuwono
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 7 No. 2 (2020): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v7i22020.74-79

Abstract

Pendahuluan: Filantin merupakan senyawa lignan utama pada Phyllanthus niruri yang menunjukkan aktivitas hepatoprotektif. Untuk keperluan standardisasi ekstrak, metode kromatografi lapis tipis (KLT)-densitometri yang sederhana dan selektif telah dikembangkan dalam penelitian ini dengan menggunakan filantin sebagai marker fitokimia. Tujuan: Untuk menetapkan kondisi analisis KLT-densitometri yang optimal dan mengevaluasi stabilitas filantin. Metode: Penentuan kondisi optimal analisis dilakukan dengan cara menentukan komposisi fase gerak, panjang gelombang analisis, waktu penjenuhan bejana, jarak elusi dan volume penotolan. Uji stabilitas filantin dilakukan dengan menganalisis larutan standar dan ekstrak pada 0, 4, 8 dan 24 jam. Hasil: Komposisi fase gerak terpilih yang menghasilkan pemisahan yang baik dan bentuk puncak yang simetris yaitu toluen : etil asetat : asam format (15 : 10,5 : 1,5 v/v/v) dengan panjang gelombang 279 nm, waktu penjenuhan bejana 60 menit, jarak elusi 8 cm dan volume penotolan 10 µL. Uji stabilitas larutan uji menunjukkan adanya penurunan area dan pergeseran Rf pada waktu penyimpanan. Kesimpulan: Pada penelitian ini telah diperoleh kondisi optimal untuk analisis filantin. Kondisi optimal yang diperoleh pada penelitian ini dapat digunakan untuk uji stabilitas tahap pre-validasi metode. Filantin tidak stabil selama penyimpanan, sehingga larutan baku dan ekstrak sebaiknya dibuat baru ketika akan dilakukan analisis untuk menjamin stabilitas.
Optimasi Metode KCKT-ELSD dengan Pemisahan HILIC untuk Penetapan Kadar Glukosamin Hidroklorida pada Suplemen Kesehatan Etik Wahyuningsih; Riesta Primaharinastiti; Mochammad Yuwono
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 7 No. 2 (2020): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v7i22020.80-87

Abstract

Pendahuluan: Analisis glukosamin hidroklorida dalam suplemen kesehatan memiliki beberapa kesulitan dikarenakan rendahnya absorpsi pada daerah UV dan sifat kepolarannya yang tinggi. Tujuan: Mendapatkan kondisi optimum metode kromatografi cair kinerja tinggi dengan detektor hamburan cahaya evaporatif (KCKT-ELSD) menggunakan kolom kromatografi cair interaksi hidrofilik (HILIC) untuk pemisahan glukosamin hidroklorida dalam suplemen kesehatan. Metode: Optimasi kondisi KCKT dilakukan terhadap pengaruh konsentrasi amonium format, pH fase gerak, dan komposisi asetonitril dalam fase gerak pada pemisahan glukosamin hidroklorida dalam suplemen makanan. ZIC-HILIC 150 x 4,6 mm, 5 μm digunakan sebagai kolom. Optimasi kondisi ELSD dilakukan terhadap pengaruh suhu nebulasi dan evaporasi. Hasil: Pemisahan terbaik yang menghasilkan puncak simetris dari glukosamin hidroklorida dicapai dalam kondisi isokratik menggunakan komposisi fase gerak asetonitril : air : 30 mM ammonium format (77 : 3 : 20, v/v/v) pada pH 4,5, suhu kolom 35°C, laju alir fase gerak 1 mL/menit, dan volume injeksi sampel 5 μL. Suhu nebulisasi dan evaporasi berturut-turut adalah 50°C dan 80°C, dengan laju aliran nitrogen adalah 1,10 standar liter per menit (SLM). Kesimpulan: Kondisi optimum KCKT-ELSD dengan mode HILIC yang diperoleh dalam penelitian ini menghasilkan kromatogram dengan puncak glukosamin hidroklorida yang simetris, dan terpisah secara selektif dan sensitif dari komponen matriks suplemen kesehatan.
Optimasi dan Validasi Metode KCKT untuk Identifikasi dan Penetapan Kadar Metabolit Nitrofuran dalam Bakso Udang Rachma Ayuningtyas; Riesta Primaharinastiti; Mochammad Yuwono
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 8 No. 2 (2021): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v8i22021.117-124

Abstract

Pendahuluan: Dikarenakan sifat dari Furazolidon, furaltadon, dan nitrofurantoin yang karsinogenik, perlu dilakukan pengawasan terhadap cemaran metabolit nitrofuran dalam produk berbahan dasar udang. Nitrofuran dimetabolisme secara cepat dalam jaringan tubuh hewan. Tujuan: Penelitian ini bertujuan melakukan validasi metode KCKT untuk penetapan kadar metabolit nitrofuran dalam bakso udang. Metode: Metabolit furazolidone, furaltadone dan nitrofurantoin yaitu 3-amino-2-oxazolidinone (AOZ), 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), dan 1-aminohydantoin (AHD) diderivatisasi menggunakan 2-nitrobenzaldehida, diekstraksi dalam etil asetat dan dicuci dengan n-heksana, kemudian disuntikkan ke dalam system KCKT dengan detektor UV-PDA. Pemisahan dilakukan dengan kolom C-18 46 x 250 mm, 5 mm. Sebagai fase gerak adalah ammonium asetat 20 mM : asetonitril (70:30), dengan laju alir 0.5 mL/menit, suhu kolom 40oC, dan volume injeksi 100 µL. Hasil: Metode yang digunakan dapat memisahkan semua metabolit dari senyawa pengotor dengan waktu analisis 40 menit, dan menghasilkan linieritas yang baik dalam rentang konsentrasi 51,90 – 103,79 ng/mL (AOZ); 56,63 - 101,39 ng/mL (AMOZ); dan 49,92 - 89,86 ng/mL (AHD). Akurasi metode dipresentasikan sebagai rekoveri dengan hasil sebesar 78,50 - 102,29% untuk AOZ; 77,02 - 99,87% untuk AMOZ; and 85,54 - 99,77% untuk AHD. Presisi metode dinyatakan dalam simpangan baku relatif (relative standard deviation, RSD) dengan hasil masing-masing adalah 6,10 - 19,90%; 3,68 - 17,75%; and 2,75 - 12,58% untuk AOZ, AMOZ, dan AHD. LOD dan LOQ sebesar 3,09 ng/mL dan 10,29 ng/mL (AOZ); 6,84 ng/mL dan 22,82 ng/mL (AMOZ); serta 4,61 ng/mL dan 15,36 ng/mL (AHD). Kesimpulan: Metode uji dapat digunakan untuk skrining awal untuk mendeteksi dan penetapan kadar cemaran metabolit nitrofuran pada bakso udang.
HPLC Method Optimization for Simultaneous Determination of Quercetin, Luteolin, Sinensetin, and Stigmasterol in Herbal Medicines Asih Imulda Hari Purwani; Riesta Primaharinastiti; Mochammad Yuwono
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 9 No. 1 (2022): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v9i12022.62-68

Abstract

Background: Quercetin, luteolin, sinensetin and stigmasterol each is the main marker compound in extracts of Sonchus arvensis, Plantago major, Orthosiphon stamineus, and Strobilanthes crispus, respectively. These extracts show nephrolithiasis activity. For quality control of herbal medicines, a high performance liquid chromatography (HPLC) method has been developed in this study using quercetin, luteolin, sinensetin and stigmasterol as phytochemical markers. Objective: to show optimal conditions of analysis and evaluate the stability of quercetin, luteolin, sinensetin and stigmasterol. Methods: The optimal conditions for analysis were carried out by determining the composition of the mobile phase, the flow rate, and the detector's wavelength. Zorbax Eclipse Plus C18 150 x 4.6 mm, 5 μm was used as the column. The stability test was done by analyzing the standard and samples stored at 4oC for 0, 3, 6 and 24 hours. Results: The best separation of the extract was achieved under isocratic conditions using a mixture of water: methanol: phosphoric acid: acetic acid : acetonitrile (50: 30: 0.05: 0.05: 20 v/v/v/ v/v) as mobile phase with detector wavelength of 352 nm, a mobile phase flow rate of 1 mL/min, and a sample injection volume of 10 μL. Conclusion: In this study, the optimal condition for analysis of quercetin, luteolin, sinensetin and stigmasterol. Quercetin, luteolin, sinensetin and stigmasterol were not stable during 6 hours storage, therefore, standard solutions and samples should be made fresh to maintain the stability.
BIOAKUMULASI LOGAM BERAT Cu OLEH Bacillus sp Riesta Primaharinastiti; A. Toto Poernomo; Noor Erma S.
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/373

Abstract

The research was conducted to investigate the ability of Bacillus sp in accumulating Cu and how much it can be acumulated. The medium used to growth the bacterium was Nutrient Broth and Atomic Absorption Spectrophotometry methods was used to assay the Cu, both in the cells and medium. The result of this study showed that Bacillus sp incubated in the Nutrient Broth medium containing 10 ppm of Cu, with continuous stirring in the room temperature was able to reduce Cu in the medium 8.912–12.623% and accumulate Cu in the cell 0.1149–0.1400 %/mg cells. Based on this result, it is necessary to develop more studies to find out what factors that influence the accumulation process and to optimize the bioprocess.
Optimization Method and Stability Test to Determinate Luteolin, Quercetin, Apigenin, and Sinensetin Levels in Herbal Medicines Using TLC-Densitometry Muhammad Hidayatullah; Mochammad Yuwono; Riesta Primaharinastiti
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 9 No. 3 (2022): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v9i32022.235-241

Abstract

Background: Nephrolithiasis is a condition in which there are one or more kidney stones in the pelvis or calyces. Luteolin, quercetin, apigenin, and sinensetin are marker compounds in the extracts of Plantago major, Sonchus arvensis, Strobilanthes crispus and Orthosiphon stamineus which have nephrolithiasis activity. To control the quality of herbal medicines, a TLC-Densitometry method was developed in this study using luteolin, quercetin, apigenin, and sinensetin as phytochemical markers. Objective: The present work aimed to develop optimal conditions for analyzing luteolin, quercetin, apigenin, and sinensetin. Methods: Determination of optimal conditions for analysis is carried out by determining the composition of the mobile phase, chamber saturation time, and analysis wavelength. Silica gel 60 F254 was used as the stationary phase. Stability tests were carried out by analyzing standards and samples at 0, 4, 8, and 24 hours. Results: The best separation that produces symmetrical peaks of herbal medicine was achieved under isocratic conditions using the composition of the mobile phase chloroform : acetone: dichloromethane : acetonitrile : formic acid (6 : 2: 2 : 0,05 : 0.05 v/v/v/v/ v) with a wavelength of 335 nm with a saturation time of 30 minutes. Conclusion: In this study, the optimal conditions for the analysis of luteolin, quercetin, apigenin, and sinensetin. Luteolin, quercetin, apigenin, and sinensetin are unstable during 8 hours of storage. Therefore, standard solutions and samples must be made fresh to maintain stability.
Physicochemical Properties and Antioxidant Activity of Three Types of Monofloral Honey from Indonesia Sulistyianingsih; Achmad Toto Poernomo; Riesta Primaharinastiti
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 9 No. 3 (2022): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v9i32022.290-297

Abstract

Background: In addition to minerals, honey contains carbohydrates (glucose and fructose), protein, amino acids, water, enzymes, ash, vitamins, and other substances. Compounds of honey can affect the chemical properties of honey. Knowing the physicochemical properties of honey is very important because physicochemical properties affect the quality of honey. One of the biological activities of honey is an antioxidant. Antioxidants can interfere with oxidative processes, prevent disease, and play an important role in the body's defence system. Objective: to determine and compare physicochemical properties (color, viscosity, ash content, water content, reducing sugar (glucose), total phenolic compound, HMF) and antioxidant activities of monofloral honey samples from Indonesia. Methods: The color of honey are categorized using the Pfund scale. Viscosity measurement is carried out using a Brookfield viscometer. The water content is carried out using a refractometer. Phenolic content and antioxidant activities analysis were carried out by UV-VIS spectrophotometer. Results: The results show that rambutan honey from Malang has the highest physicochemical properties and antioxidant activity, which had an amber color, water content of 21.7% b/b, acidity 20.7 mL NaOH/Kg, viscosity of 33.08 poise, ash content of 0.17% b/b, reducing sugar 69.38%b/b, total phenolics content 533.7 mg GAE/Kg sample and IC50 0.111 µg/mL. Conclusion: The quality of honey varies from region to region. The best honey (according to SNI) is rambutan honey from Malang.
Co-Authors A. Toto Poernomo Achmad Toto Poernomo Asih Imulda Hari Purwani Asri Darmawati Bambang Tri Purwanto Djoko Agus Purwanto Estu Mahanani Dhilasari Etik Wahyuningsih Fajar Jamaluddin Sandhori Febri Annuryanti Firmansyah Ardian Ramadhani Hanif Rifqi Prasetyawan Idha Kusumawati Ika Rizki Helwandi Isnaeni Lailatul Nuraini Laili Irfanah Mochamad Yuwono Mochammad Yuwono Muhammad Hidyatullah Noor Erma S. Rachma Ayuningtyas Rachma Nurhayati Soraya Aulia Subhan Rullyansyah Sulistyianingsih Syahrani, Achmad