Denta: Jurnal Kedokteran Gigi
The Denta (Jurnal Kedokteran Gigi) accepts original manuscripts relating to the field of dentistry, including: research reports, case reports and literature reviews. The spread of dental fields comprise: -Dental material -Endodontics -Forensic dentistry -General dentistry -Oral and maxillofacial surgery -Oral biology -Oral medicine -Oral pathology -Orthodontics -Pediatric dentistry -Periodontics -Prosthodontics -Public health dentistry -Radiographic dentistry -Biomarine Science in Dental field
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<![CDATA[Bioviability of Mangrove Leaves Extract (Sonneratia alba) on Fibroblast BHK-21 Cell Culture ]]>
<![CDATA[Arlita Gladys Tricia Charyadie]]>;
<![CDATA[Aprilia]]>;
<![CDATA[Widyastuti]]>
<![CDATA[ Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Hang Tuah ]]>
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<![CDATA[Background: Dental caries is a major oral health problem which is most commonly found in Indonesia. One of the main factors in role of causing dental caries is Streptococcus mutans which is the primary target of the prevention of caries. Leaf extract of Sonneratia alba has been proven to have an antimicrobial effect towards Streptococcus mutans, but its cytotoxic activity on normal cells is still unknown. The initial cell viability in this study was conducted using cell line BHK-21. Purpose: The aim of this study is to determine the cell viability of fibroblast cells line BHK-21 by given Sonneratia alba’s leaf extract. Materials and Methods: The experiment was conducted using post test only control group design. BHK-21 fibroblast cell culture on 96 wells were divided into cell control group (n=9), media control group (n=9) and test groups (n=9). The test groups were given various concentration of Sonneratia alba’s leaf extract, they were: 5%; 10%; and 20%. The cell cultures were incubated for 24 hours before and after treatment using Sonneratia alba’s leaf extract. After dripping MTT into the microplates, OD were read using ELISA reader and the viability were counted. The data that was acquired from the viability calculation was statistically analyzed using One Way ANOVA and LSD test. Results: Data showed the increase of viability cell in all the test groups. The highest viability cell showed by the test group with 10% concentration of Sonneratia alba (153,7122%±7,42458), and the lowest found in the test group with 20% concentration of leaf extract (141,4682%±7,48752). Conclusion: In this experiment, the leaf extract of Sonneratia alba proved to be nontoxic on fibroblast cells line BHK-21, the extract increased the viability and also caused proliferation of fibroblast cells.]]>
<![CDATA[Combination Administration of Stichopus hermaniiGel and Hyperbaric Oxygen Therapy the number of fibroblasts in diabetes mellitus rats with periodontitis ]]>
<![CDATA[Faraziza Maulana]]>;
<![CDATA[Dian Mulawarmanti]]>;
<![CDATA[Fanny M.Laihad]]>
<![CDATA[ Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Hang Tuah ]]>
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<![CDATA[Background: Periodontitis is the most severe complication in oral cavity to diabetes mellitus. Stichopus hermanii contain GAG, a polysaccharide which very useful in wound healing. Hyperbaric oxygen therapy is effective repairing ischemia, hypoxia, and is used as adjuvant therapy. Purpose: To determine effect of administration of Stichopus hermanii (SH) 3% gel and hyperbaric oxygen therapy 3x30 minutes with 5 minute intervals in 7 consecutive days to the number of fibroblasts in ligament periodontal diabetes mellitus rats with periodontitis. Materials and Methods: The experiment design is factorial design. Rats are inducted with STZ single dose 65 mg/kgBB to make them suffer DM and are inducted with P.gingivalis 1x109sel/ml for 5 days straight to make them suffer periodontitis. 20 male wistar rats are divided into 5 groups. K- (control), K1 (STZ+P.Gingivalis), K2(STZ+P.Gingivalis+SH), K3(STZ+P.Gingivalis+HBO), K4(STZ+P.Gingivalis+SH+HBO). After 52 days treatment, rats is terminated to make a HPA Sample from the mandible and fibroblast is counted with 400x magnification. Result: One-way Annova here were significant differences (p<0,05) in K- (32.300 ± 11.381), K1 (31.725 ± 5.590), K2 (54.225 ± 7.165), K3 (49.725 ± 10.088), K4 (72.075 ± 9.342). Conclusion: administration of Stichopus hermanii 3% gel combination and 2.4 ATA hyperbaric oxygen 3x30 minutes a day with 5 minutes intervals in 7 consecutive days can increase the number of fibroblasts in diabetes mellitus rats with periodontitis.]]>
<![CDATA[Effect of Hyperbaric Oxygen Therapy Combined with Golden Sea Cucumber (Stichopus hermanii Powder to the Level Of Blood Glucose Diabetic Wistar Rat Induced Porphyromonas gingivalis ]]>
<![CDATA[Rafika Rusydia Darojati]]>;
<![CDATA[Yoifah Rizka]]>;
<![CDATA[Syamsulina Revianti]]>
<![CDATA[ Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Hang Tuah ]]>
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<![CDATA[Background: Diabetes mellitus (DM) is a metabolic disorders characterized by hyperglycemia caused by oxidative stress. Porphyromonas gingivalis (P.g) is a periodontopathogen caused periodontitis exacerbate blood glucose levels (BGL). Hyperbaric oxygen therapy (HBOT) combined with stichopus hermanii (SH) has a role as an antioxidant and antibacterial agent. Purpopse: To analysis effect of HBOT, SH powder and combination of both to the level of blood glucose diabetic Wistar rat induced P.g. Materials and Methods: This study was pre-test-post-test control group design, using 20 male Wistar rat were divided into 5 groups, Group1 (STZ), Group2 (STZ+P.g), Group3 (STZ+P.g+HBOT), Group4 (STZ+P.g+SH powder), Group5 (STZ+P.g+HBO+SH). STZ induction intravenous for 4 days in a row. P.g. induction was exposure 3 times for 4 days. After therapy (7 days), sample blood tail vein of rat were taken for measure BGL by glucometer. Result: Level of blood glucose K1(280.25±88.786)mg/dl, K2(372.25±135.662)mg/dl, K3(249.50±96.652)mg/dl, K4(172.00±82.077)mg/dl, K5(104.75±38.257)mg/dl. Conclusion: Induction of STZ increased the BGL but induction of P.g. cannot exacerbate BGL . HBOT combined with SH powder can reduce BGL in STZ and P.g. induction group.]]>
<![CDATA[Effect of Stichopus Hermanni to Bigonial Width on Maxillary Suture Expansion using Cephalometric Analysis ]]>
<![CDATA[Kristin Gaby Rosari]]>;
<![CDATA[Noengki Prameswari]]>;
<![CDATA[Lisdiana Mardanus]]>
<![CDATA[ Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Hang Tuah ]]>
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<![CDATA[Background:Maxillary expansion uses mechanical forces to open the maxillary suture which causes inflammation and bone remodeling around the area. Stichopus hermanni is known to contain flavonoid, chondroitin sulfate, and saponin which is used to inhibit inflammatory process, increase bone metabolism and mineralization, and wound healing. Purpose: The aim of this experiment is to know the effect of Stichopus hermanni to bigonial width on maxillary suture remodeling expansion using cephalometric analysis. Materials and Methods: Experimental units used were 24 male Cavia cobaya divided into 4 groups (n=6). Group K(-) is negative control. Helical spring was applied and activated in the other 3 groups for 10 days. Group K(+) was given 2% NaCMC gel, both group P1 and P2 was given 3% Stichopus hermanni gel. Group P2 were also given retention period for 10 days after activation period. Cavia cobaya were then decapitated and observed for the bigonial width changes. The data was analyzed with One Way ANOVA test continued with LSD test. Results: The result of ANOVA test showed siginficant differences among the treatment groups p=0,000 (p<0,05). LSD test showed significant differences between group K(-) and K(+) (Sig. 0,005), K(-) and P1 (Sig. 0,001), and group K(-) and P2 (Sig. 0,000). Conclusion: 3% Stichopus hermanni gel increases bigonial width but not significant in remodeling process on maxillary suture expansion.]]>
<![CDATA[Effectivity of the Gold Sea Cucumber Supplementation (Stichopus hermanii) of Catalase Activity in Wistar Rat’s Submandibular with Oral Candidiasis ]]>
<![CDATA[Ajeng Hanun Winny Khairina]]>;
<![CDATA[Syamsulina Revianti]]>;
<![CDATA[Nafi’ah]]>
<![CDATA[ Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Hang Tuah ]]>
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<![CDATA[Background: Oral candidiasis is common opportunistic infection in oral cavity caused by the fungal pathogen Candida albicans and it can develop to malignant such as a candida leukoplakia if it’s affected by cigarette smoke exposure. Purpose: To analysis the effectivity of Stichopus hermanii supplementation of catalase activity in Wistar rats submandibular with oral candidiasis. Materials and Methods: This study was post-test-only control group design, using 35 male Wistar rats were divided into 5 groups, Group1 is negative control, Group2 is positive control, Group3 is treatment control with Stichopus hermanii 0,0225 mg/g BB powder, Group4 is treatment control with Stichopus hermanii 0,045 mg/g BB powder) Group5 is treatment control with Stichopus hermanii 0,09 mg/kg BB powder). Oral candida infection was achieved by spray and cotton swab rolled over all parts of the mouth. After treatment, rats were euthanized and submandibular were biopsied to make histopathology slide for measure activity of enzim catalase with spectrophotometer λ 280 nm. Data were analyzed using One Way ANOVA and Tukey LSD test. Results: There are significantly differences catalase activity between K1&K2, K2&K3, K4&K1, K2&K4, K2&K5. Conclusion: Suplementation of Stichopus hermanii effectiv of catalase activity in wistar rat’s submandibular with Candida albicans A infection.]]>
<![CDATA[Effectiveness of Chitosan Solution as Denture Cleanser to Inhibit the Growth of Candida albicans on Acrylic, Valplast and Luciton-FRS ]]>
<![CDATA[Anindita Apsari]]>;
<![CDATA[Vivin Ariestania]]>
<![CDATA[ Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Hang Tuah ]]>
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<![CDATA[Background: Heat cure acrylic and nylon thermoplastic valplast and lucitone-FRS brands are the three materials most often used as denture bases. Candida albicans is the dominant microorganism that can cause denture stomatitis. Cleaning denture can be done in two ways, namely the mechanical way with a toothbrush or an ultrasonic cleanser and a chemical method by immersing the denture into the cleaning solution. Chitosan solution is antibacterial and anti fungal which can inhibit the growth of Candida albicans colonies on heat cured acrylic plates, valplast and lucitone-FRS. Purpose: To determine the concentration of chitosan solution and the type of denture plate that most effectively inhibited the growth of Streptococcus mutans Candida albicans colonies on heat cured acrylic, valplast and lucitone-FRS plates. Materials and Methods: 21 heat cured acrylic plate samples, 21 valplast plate samples, 21 lucitone-FRS plate samples measuring 10x10x2 mm divided by 9 groups. Heat cured, valplast and lucitone-FRS acrylic plate samples were contaminated with Candida albicans then immersed using the concentration of 0.25%, 0.5% chitosan solution and sterile aquades as the control group with 90 minutes immersion time. The entire study sample was calculated using Candida albicans colonies on Sabourroud's Dextrose Agar media. Results: The Kruskall Wallis test showed a significant difference (p <0.05) in all treatment groups. Mann-Whitney showed a significant difference (p <0.05) in all groups in the Candida albicans study. Conclusion: Soaking the lucitone-FRS plate in 0.5% chitosan solution for 90 minutes was the most effective in inhibiting the growth of Candida albicans colonies.]]>
<![CDATA[Effectiveness of PRP (Platelet Rich Plasma) To Increased BMD (Bone Mineral Density) At Maxilla Dental Implant Placement with 3-Dimensional Radiographic Examination (CBCT) ]]>
<![CDATA[Dianty Saptaswari]]>;
<![CDATA[Yoifah Rizka Wedarti]]>;
<![CDATA[Hansen Kurniawan]]>
<![CDATA[ Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Hang Tuah ]]>
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<![CDATA[Background: Dental implant is a device that is surgically implanted into the soft tissue or into the jaw bone. dental implants have become the treatment of choice for replacing missing teeth. Platelet rich plasma and platelet concentration derived from autologous blood containing autologous growth factors in which the platelet rich plasma can improve wound healing and increase the regeneration of bone. today cone beam computed tomography (CBCT) has been available for 3D visualization of craniofacial section. CBCT 3 D is able to provide information on the general bone mineral density. Purpose: The purpose of this studywas to determine thebone mineral density by three-dimensional radiographic picture after dental implant placementbyan additional plateletrich plasma. Material and Method: This study was divided into 2 groups. The first placement of a dental implant with platelet rich plasma and the second placement of a dental implant without platelet rich plasma. Results: there are significant differences in dental implant placement with platelet rich plasma and placement of dental implants without platelet rich plasma. In the test results obtained Independent T test (p = 0.02). Conclusion: There was an increase bone mineral density in dental implant placement with platelet rich plasma were performed using three-dimensional radiographic examination (CBCT).]]>
<![CDATA[Role of C-type Lectin Receptor to C.albicans on Promotion of Inflamantory Diseases ]]>
<![CDATA[Erna Sulistyani]]>
<![CDATA[ Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Hang Tuah ]]>
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<![CDATA[In early 2010 there was a very rapid progress in the development of immunity to fungi, i.e the discovery of CLR. CLR is one of the PRR receptors found in immune cells that recognize the cell wall of fungi and then trigger a transduction signal that eventually provoke the production of various proinflamatory include IL1β, IL6, TNF α and induce polarization Th17. In the condition of infection the number of innate immune cells increases dramatically, lead to increasing of cytokines produced. Both cytokines and the cells that produce them enter the bloodstream. If there is unbalance level of pro and anti inflammatory cytokine, various inflammatory and/or autoimmune diseases can be occur. Unlike the focal concept infection, where the agent will cause disease if enters the blood vessels, through this concept a fungal infection at epithelium can lead to inflammatory diseases in other part of body without having to go through fungus]]>
<![CDATA[(Study of Hyperbaric Oxygen Theraphy and Gold sea Cucumber (Stichopus Hermanii) 3% for Collagen Density of Rat’s with Diabetes Mellitus Induced by Porphyromonas Gingivalis Bactery ]]>
<![CDATA[Dyah Lestarining R]]>;
<![CDATA[Dian Mulawarmanti]]>;
<![CDATA[Widyastuti]]>
<![CDATA[ Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Hang Tuah ]]>
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<![CDATA[Background: Periodontitis is complication of DM in chronic state. Periodontitis susceptibility increase threefold in patient DM compared non-DM. Debridement with SRP alone is less effective in DM. Hyperbaric Oxygen Therapy (HBO) has the effect of increasing insulin sensitivity and synthesis of collagen. Collagen is the largest extracellular matrix supporting periodontal tissue. Stchopus hermanii contains condrotin sulfate serves to inhibit the formation of mixed periodontopatogen bacteri. Purpose: To determine the therapeutic effects of HBO 2,4 ATA 3x3’ interval 5’ 7 days and stichopus hermanii against periodontal ligament collagen density in the healing process of periodontitis with DM. Materials and Methods:The experiment design is factorial design. Rats are inducted with STZ 65 mg/kgBB to make them suffer DM and are inducted with P.gingivalis 1x109sel/ml for 5 days straight to make them suffer periodontitis, 20 male Wistar rats were divided into 4 groups. K0 (control group), K1(STZ+P. gingivalis), K2(STZ+P. gingivalis+Stichopus hermanii), K3(STZ+P. gingivalis+HBO), and K4(STZ+P.gingivalis+stichopus hermanii HBO) after treatment on day 52, all groups were sacrificed and done making preparations for the HPA of the mandible bone. Results: Data were analyzed using chi-square, Kruskal wallis, and Mann Whitney U with 95% significance level (p = 0.05). increase level of collagen significantly K1(1 ± 0.00) - K2(2±0.00), dan K1(1 ± 0.00) - K4(2.5 ± 0.289).Conclution:Therapy HBO combination of 2.4 ATA 3 x 30 with 5 minutes air breaks for 7 days and gel Stichopus hermanii3% effective on the Densityof collagen in the periodontal ligament healing process periodontitis with DM.]]>
<![CDATA[Increasing Performance Treatment Index’s Score (PTI) of Elementary School Children in Keputih-Surabaya Through Dental Public Health Warrior Project ]]>
<![CDATA[Aulia Dwi Maharani]]>
<![CDATA[ Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Hang Tuah ]]>
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<![CDATA[Introduction:The Dental Public Health Warrior (DPHW) project is a social activity which provides a creative, fun, and interactive dental health education throughout elementary schools in Keputih Area-Surabaya,Indonesia. Purpose: The aim of this study was to determine the effectivity of those projects in increasing the Performance Treatment Index of elementary school’s student. PTI score used to measure the willingness to treat their teeth. Materials and Methods:In this pre experimental study (pretest and posttest only design), subjects were 54 elementary students (4th grade) . All subjects were educated by the DPHW team and their DMF-t score and PTI score were collected by calibrated dentists before the education and in 3 months after the education. Results: The mean of DMF-t score increase from 6,7 to 6,9. The mean of PTI score increase from 3,7 to 9,5 . Wilcoxon analysis showed there is significant different between before and after education (p= 0,001). Conclusion:. The DPHW project can be effectively used to increase the Performance Treatment Index of elementary school’s student. The increase of PTI indekx means there is increase in willingness to treat their teeth.]]>
