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DENTA JURNAL KEDOKTERAN GIGI Jl. Arief Rahman Hakim No.150, Keputih, Kec. Sukolilo, Kota Surabaya, Jawa Timur 60111 Gedung F6 Fakultas Kedokteran Gigi Telp. 031-5945864
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Denta: Jurnal Kedokteran Gigi
Published by Universitas Hang Tuah
ISSN : 19075987     EISSN : 26151790     DOI : https://doi.org/10.30649/denta.v16i1
Core Subject : Health,
Dentistry
The Denta (Jurnal Kedokteran Gigi) accepts original manuscripts relating to the field of dentistry, including: research reports, case reports and literature reviews. The spread of dental fields comprise: -Dental material -Endodontics -Forensic dentistry -General dentistry -Oral and maxillofacial surgery -Oral biology -Oral medicine -Oral pathology -Orthodontics -Pediatric dentistry -Periodontics -Prosthodontics -Public health dentistry -Radiographic dentistry -Biomarine Science in Dental field
Arjuna Subject : Kedokteran Gigi - Kedokteran Gigi (Lain-Lain)
Articles 13 Documents
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Search results for , issue "Vol 9 No 2 (2015): Agustus" : 13 Documents clear
Bioviabilitas Hidroksiapatit Ekstrak Cangkang Kerang Darah (Anadara granosa) Terhadap Sel Punca Mesenkimal Sebagai Bahan Graft Tulang Arlita Dewi Nastiti; Widyastuti; Fanny M Laihad
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: One of a bone defect fillers is ahydroxyapatite. Hydroxyapatite can be obtained from Anadara granosa shells extract that have high calcium content. The mineral content can be used as a bone filler material for bone grafting. However, the material is not known bioviability to against periodontal tissues so that was needed bioviability testing. This bioviability testing using mesenchymal stem cells as mesenchymal stem cells can differentiate into periodontal tissues. Purpose: To determine bioviability Anadara granosa shell extract on mesenchymal stem cells. Materials and Methods: This study was conducted using post-only control group design. Mesenchymal stem cells in 96 wells were divided into a control group of cells (n=7), media controls (n=7), and treatment (n=7). The treatment group were given various doses of the Anadara granosa shell extract with a concentration 54 mg/ml, 27 mg/ml, 13.5 mg /ml and 6.75 mg/ml. The mesenchymal stem cells were incubated for 24 hours before and after treatment. Once given MTT, the optical density is read by ELISA reader and calculated the percentage of viability. The cell viability data were analyzed with KruskalWallis statistical test, Mann-Whitney. Results: From the results showed that an increase in cell viability to Anadara granosa shell extract. Increased cell viability starting treatment group concentration of 54 mg/ml (23,,67%), 27 mg/ml (57,43%), 13,5 mg/ml (68,87%), 6,75 mg / ml (81,92%). The highest cell viability at concentrations of 6,75 mg/ml (81,92%). Conclusion: Bioviability extract blood clam Anadara granosa shell have the highestconcentration of 6,75 mg/ml and bioviabilitas lowest cell at a concentration of 54 mg/ml.
Daya Hambat Minyak Hati Ikan Hiu Terhadap Pertumbuhan Bakteri Porphyromonas gingivalis Felisitas Maria Agustina; Dian Mulawarmanti; Yoifah Rizka Wedarti
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: Porphyromonas gingivalis is the dominant bacteria that found in chronic periodontitis. Conventional antibacterial (antibiotic) especially tetracycline mostly used for periodontal treatment, but antibiotic ussage often causes resistant, allergy, and toxic. Shark Liver Oil Cenctrophorus sp species have antibacterial effect through its contents, squalene and squalamine that can be developed as an antibacterial adjuvant therapy in periodontal disease. Purpose: To examine the inhibition effect of Shark Liver Oil to the growth of Porphyromonas gingivalis. Materials and Methods: This study used a research design post test only control group design. The research subject is Porphyromonas gingivalis strain ATCC 33277 which have total amount 30 samples, divided into 5 groups. Three groups were given the shark liver oil with different concentrations of 10%, 15%, and 20%. Positive control were given the tetracycline and negative control were given the DMSO 1%. The inhibition was examined using diffution method and was inoculated on MH agar. The inhibitory effect has been observed by measuring the diameter of clear area on the disk using digital calipersin milimeter. Result: Shark liver oil could inhibit the growth of Porphyromonas gingivalis in every concentrations. Concentration 10% with 9,06 mm, concentration 15% with 11,31 mm, concentration 20% with 13,2 mm, while the negative control with 6,01 mm, and positive control with 30,09 mm. Conclusion: Shark liver oil could inhibit the growth of Porphyromonas gingivalis.
Efektivitas Antibakteri Ekstrak Daun Mangrove Acanthus ilicifolius Terhadap Biofilm Enterococcus faecalis Hariningtyas Dian Rachmawati; Aprilia; Kristanti Parisihni
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: Enterococcus faecalis is one of the bacteria which have ability to form biofilms that can cause persistent endodontic infections. Biofilm is a communities of well-organized bacteria adhere to the surface and coated by layer of extracellular polysaccharide matrix. The extracts of mangrove Acanthus ilicifolius leaves have been known to have potentially bioactive compounds as antibacterial such as saponins, alkaloids, terpenoids, and tannins. Purpose: The aim of this study was to determine the antibacterial effectiveness of mangrove Acanthus ilicifolius leaves extracts to Enterococcus faecalis biofilm. Materials and Methods: This study was true experimental laboratory with post test only control group design. The sample in this study was Enterococcus faecalis, divided into 6 groups consisted of control positive (NaOCl 2.5%) group, and 5 treatment groups of mangrove Acanthus ilicifolius leaves extract with different concentration of 60 mg/ml; 70 mg/ml; 80 mg/ml; 90 mg/ml; and 100mg/ml and be repeated 8 times. Enterococcus faecalis bacteria were cultured in mediaTSBglu incubated for 24 hours. Amount of 0.1 ml of bacteria Enterococcus faecalis with concentrations of 106were loaded on microtiter plate then stained with crystal violet. Biofilms checked by measuring optical density (OD) using an ELISA reader. Data were analyzed by One-way ANOVA followed by Post Hoc test. Result: Acanthus ilicifolius leaves extract decrease the OD of Enterococcus faecalis biofilm (p<0,05) in all groups. Conclusion: The extract of mangrove Acanthus ilicifolius leaves extract have antibacterial efficacy against Enterococcus faecalis biofilm.
Efek Proteksi Ekstrak Etanol Stichopus hermanii Terhadap Jumlah Limfosit pada Tikus yang Terpapar Asap Rokok dan Diinduksi Candida albicans Auliasari Yunanda; Syamsulina Revianti; Isidora Karsini S
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: Smoking is associated with oral fungi which can causes oral candidiasis. Stichopus hermanii contains of antioxidant, antifungal and immunostimulating agent. Purpose: To evaluate the protection effect of Stichopus hermanii ethanol extract to lymphocytes amount in Wistar rat with CS exposure and C.albicans infection. Material and Method: This experiment were used post test-only control groups design. We used 42 male Wistar rats and divided into 7 groups. 1st group (saline 0,1mL, fresh air, CMC-Na 0,2%), 2nd group (saline 0,1mL, CS, CMC-Na 0,2%), 3rd group (C.albicans 0,1mL, fresh air, CMC-Na 0,2%), 4th group (C.albicans 0,1mL, CS, CMC-Na 0,2%), 5th group (saline 0,1mL, CS, Stichopus hermanii extract 0,02mg/kgBW), 6th group (C.albicans 0,1mL, fresh air, Stichopus hermanii extract 0,02mg/kgBW), 7th group (C.albicans 0,1 mL, CS, Stichopus hermanii extract 0,02mg/kgBW). Wistar rats has been infected by C.albicans for a week, exposed by CS for 8 weeks, and application of Stichopus hermanii extract for 8 weeks period. Furthermore, Wistar rat has been euthanized after 8 weeks of experiments. The amount of lymphocytes was counted through peripheral blood smear method using Diff-count under light microscope with 1000 times magnification. Data were collected and analyzed using Kruskal-Wallis test and Mann-Whitney test. Result: CS exposure and C.albicans infection group can decreaselymphocytes amount. Suplementation groups with Stichopus hermanii extract can increase lymphocytes amount. Conclusion: Supplementation with Stichopus hermanii extract has protective effect to stimulate lymphocytes proliferation in Wistar rat after CS exposure and C.albicans infection.
Efektivitas Topikal Aplikasi Fluoride Menggunakan Ekstrak Teh Hijau Dibandingkan dengan Sodium Fluoride Pada Gigi Sapi Ekky Berliana R P; Istien Wardani; Eriza Juniar
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: Prevention of caries and periodontal disease by performing dental health improvement has become a major destination in the world of dentistry. Topical application of fluoride is one of the most effective ways to prevent caries, fluoride inhibits the absorption of salivary proteins on the surface of the email so the pellicle and plaque formation reduced,increase enamel remineralization, resistance to acids and decreased pH. Topical application of fluoride commonly used is sodium fluoride 0.1%. Green tea (Camellia sinensis) can be an alternative herbs as a topical application of fluoride because it has reduced the growth of plaque and have antibacterial. Purpose: To determine the effectiveness of topical fluoride applications using green tea extract compared to sodium fluoride on the teeth cow. Materials and Methods: This study was true experimental laboratories with post test only control group design. The subjects in this study was a teeth cow (Bovine) is applied with the topical application of fluoride, control group without administration of topical application offluoride, treatment group one with Sodium fluoride 0.1%, and the treatment group two with green tea extract (Camellia sinensis) 0.1%. Counting the amount of fluoride by Energy Dispersive X-ray Spectrophotomrtry (EDS). The data was processed by Levene test and one way ANOVA. Result: There are no significant differences in the number of fluoride among all groups (P> 0.05). Conclusion: There were no significant differences in the effectiveness of topical application of fluoride using green tea extract (Camellia sinensis) concentration of 0.1% compared with 0.1% Sodium fluoride on teeth cow (Bovine.
Pengaruh Induksi Aspergillus niger/brasiliensis Strain ATCC ® 16404™ Secara Sistemik dan Pencabutan Gigi Terhadap Jumlah Koloni pada Mukosa Gingiva Yanuardi Kristandia; Fanny M Laihad; Astrid Palmasari
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: Prevention of gingival mucosal tissue damage caused by aspergillus niger the invasive fungal infection in the mouth is still difficult to determine its diagnosis and therapy. The cause of maxillary gingiva mucosal damage can be indicated as systemic fungal infections triggered by tooth extraction. There have been no research yet about the effect of invasive fungal aspergillus niger infections in the maxillary mucosa that has been performed tooth extraction and no tooth extraction. Purpose: To determine the effect of Aspergillus niger/brasiliensis strain ATCC ®16404 ™ induction systemically and tooth extraction action to the number of colonies on the maxillary gingiva mucosa. Materials and Methods: This study used post test only control group design. Thirty two adult male wistar rats were randomly divided into 4 groups: group K-, group P1 had tooth extraction, group P2 injected 0.3 ml by Aspergillus niger strain ATCC®16404 ™ 0.5 Mc Farland, P3 had extraction of maxillary tooth and injection 0.3 ml of the fungus aspergillus niger strain ATCC®16404 ™ 0.5 Mc Farland. Swabbing were applied on each group (day 1,3,5) in the maxillary mucosa and cultured on saboround dextrose agar (SDA) media with the spreader technique and incubated (37°C) for 48 hours Data were analyzed by Kruskal Wallis. Result: There is no significant difference in the amount of the fungus aspergillus niger in each group. Conclusion: Induction of aspergillus niger systemic wasn’t able to lead to significant conditions of the oral cavity, and therefore revocation isn’t a factor that that triggered the severity of the onset of aspergillus niger.
Pengaruh Pemberian Gel Teripang Emas Terhadap Jumlah Osteoklas Di Daerah Tekanan Pada Remodeling Tulang Pergerakan Gigi Ortodonti Stefany Wijaya; Noengki Prameswari; Maria Lisdiana T
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Orthodontic treatment takes a long time for bone remodeling. Stichopus hermanii has various compounds such as EPA-DHA, calcium, magnesium, f lavonoid and triterpenoid, that may reduce the number of osteoclasts. Research Stichopus hermanii against osteoclasts in bone remodeling has not been investigated. Purpose: This study aimed to verify the effect of Stichopus hermanii gel to the number of osteoclasts in bone remodeling pressure area of orthodontic, tooth movement. Materials and Methods: Forty-eight male Cavia cobaya were divided into eight groups. (K1) and (K3) negative control for 6 and 14 days. (K2) and (K4) positive control for 6 and 14 days by provision of separator and NaCMC gel. (P1), (P3) and (P2), (P4) were treated for 6 and 14 days by provision of separator and Stichopus hermanii gel 3% and 3.5%. Osteoclasts were examited calculated by histological preparations with HE staining and viewed using a light microscope with 400x magnification, and then analyzed by ANOVA followed by LSD test. Result: In analysis descriptive the mean of K1, K2, K3, K4, P1, P2, P3, and P4 are (3,33 ± 1,211), (10,67 ± 1,211), (8,33 ± 0,816), (4,33 ± 1,211), (3 ± 0,894), (14,3 ± 1,633), (9,33 ± 1,366), and (12,67 ± 2,338) ANOVA showed a significant difference in the number of osteoclasts. LSD test showed significant differences in the number of osteoclasts in all groups. Conclusion: Stichopus hermanii gel with a concentration of 3,5% more effective on day 6, and the concentration of Stichopus hermanii gel 3% is more effective on the 14th day by reducing the number of osteoclasts in bone remodeling pressure area orthodontic tooth movement.
Pengaruh Terapi Oksigen Hiperbarik Terhadap Jumlah Sel Osteosit pada Daerah Tekanan Saat Pergerakan Gigi Ortodonti Ivan Nicholas Jonathan; Arya Brahmanta; Pambudi Rahardjo
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Backgrounds: Orthodontic tooth movement occurs as the result of mechanical force to teeth. Many cells are playing part to correspond the mechanical force, which one of them is osteocyte. Orthodontic force in pressure side induces osteocyte cell death. Hyperbaric oxygen therapy (HBOT) has many benefits, which one of them increases NO, of which can prevents osteocyte apoptosis. Purpose: To determine the influences of 3x30 minutes with 5 minutes break, 2,4 ATA HBOT for 5 and 7 days to osteocyte numbers on pressure side during orthodontic tooth movement. Materials and Methods: This study used “post test only control group” design, 42 guinea pigs were divided into 4 groups. K(-) was a negative control group, K(+) was the orthodontic group, and K1 & K2 were groups that given both orthodontic and HBOT for 5 and 7 days. Osteocyte numbers were counted after HE preparation. Data were analyzed by one way ANOVA and LSD test. Result: Data showed a significant difference of osteocyte amounts between K(-) group and K(+) group and between K(+) with K1 and K2 groups. Conclusions: HBOT for 5 days as well as 7 days gives a significant influences for the increase of osteocyte numbers on pressure side during tooth movement in Cavia cobaya.
Perbandingan Hasil Penilaian Ketebalan Korteks dengan Menggunakan Mental Index pada Pasien Wanita Berdasarkan Kelompok Umur 30-70 Tahun Edward Perdana Putra; Sarianoferni; Endah Wahjuningsih
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: The osteoporosis risk can be detected through the width of cortical mandible and porosity which can be seen in panoramic radiography. One of measurement methods using panoramic radiography in assessing the quality of bone and observing symptom of resorption and osteoporosis by utilizing Mental Index. Mental Index is a method in counting the average width of cortex under foramen mentale because thin mandible might correlates with decreasing of bone density. Purpose: To compare the assessment result of the cortex thickness by using Mental Index on women patient based on age of group (aged 30-70) at RSGM FKG Hang Tuah University, Surabaya. Materials and Methods: In this research, panoramic radiography of women patients aged 30 to 70 were used with utilizing radiomorfometri Mental Index to assess the mandibular cortical thickness manually using adigital caliper (accuracy of 0,01mm). Result: Based on One Way ANOVA assessment, p=0.000 value was found in which it means that there was a significant difference from the result of cortical thickness assessment in age of groups using Mental Index based onpanoramic radiography of women patients aged 30-70 at RSGM FKG Hang Tuah University, Surabaya. Conclusion: The assessment of cortical thickness by using Mental Index on women patients who experienced a decrease bone density in the age of 56-70 years old and which is compared with previous three groups of age, 30-35 years old, 36-45 years old and 46-55 years old and out of 93 panoramic radiographies, 11 panoramic radiographies were detected osteoporosis or the percentage of osteoporosis occurance based on Mental Index was 12%.
Perbedaan Efektivitas Ekstrak Daun Mangrove Acanthus ilicifolius Dengan Sodium Bikarbonat 5% Terhadap Penurunan Jumlah Koloni Candida albicans Pada Perendaman Nilon Termoplastik Aviyuda Prabowo; Paulus Budi Teguh; Dwi Andriani
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: Nylon thermoplastic is denture base materials are often used. Dentures cleansing immersion can reduce calculus accumulation and Candida albicans (C.albicans) attachment. Sodium bicarbonate 5% is commonly used but has flaws. Acanthus ilicifolius leaves can be alternative herbal ingredients for denture cleanser because it has potency as antifungal and antibacterial. Purpose: To compare the difference in the effectiveness of antiC.albicans between Acanthus ilicifolius leaf extract and sodium bicarbonate 5% on nylon thermoplastic immersion. Materials and Methods: This study was true experimental laboratories with post test only control group design. The subjects in this study was a nylon thermoplastic soaked for 5 minutes and were divided into 5 groups: negative control group (DMSO 1%), positive control group (Sodium Bicarbonate 5%), and treatment groups (Acanthus ilicifolius leaf extract 1% , 0.5%, 0.25%). 0.1 ml of Sabouraud broth that has been contaminated with C.albicans in the immersion nylon thermoplastic then cultured in media SDA and incubated for 24 hours. Colony counted by colony counter in CFU/mL. The datawas processed by one way ANOVA and followed by LSD test. Result: There were differences in the number of colonies of C.albicans significantly between treatment groups with the negative control extract 1% and 0.5% (P <0,05) there is no significant difference between the negative control group with treatment group 3 (P3) (P>0,05). Conclusion: There were differences in the effectiveness of anti-C.albicans of Acanthus ilicifolius leaf extract concentration of 1% and 0,5% with sodium bicarbonate 5% on nylon thermoplasticimmersion

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