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Makara Journal of Health Research
Published by Universitas Indonesia
ISSN : 23563664     EISSN : 23563656     DOI : https://doi.org/10.7454/msk
Core Subject :
Makara Journal of Health Research (MJHR) is a peer-reviewed and scientific journal published by Universitas Indonesia. Starting from 2019 onwards, MJHR is redirecting its scope, focusing on publishing research articles relevant to non-communicable diseases (NCDs) addressing issues of surveillance, disease management and health policy in the Asia Pacific and Mediterranean countries. Relevant academic articles regarding NCDs analyzed from an integrated perspective including individual and population level, experimental and clinical approaches, epidemiology, public health, disease prevention and health promotion, diagnosis, treatment, prognosis and palliative care are welcome.
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Articles 5 Documents
 1 
Search results for , issue "Vol. 20, No. 1" : 5 Documents clear
Analysis Expression of ZIP1 and Caspase-3 Protein in Adenocarsinoma of the Prostate Septiawan, Aditya D; Kodariah, Ria; Saraswati, Meilania
Makara Journal of Health Research Vol. 20, No. 1
Publisher : UI Scholars Hub

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Abstract

Background: Carcinogenesis of adenocarcinoma of the prostate occurs due to dysregulation of zinc level within the cells. Intracellular zinc molecules influx is regulated by a transporter protein ZIP1, whose non-presence is predicted to inhibit apoptosis, thus leads to the development of prostate adenocarcinoma. Methods: This study was aimed to analyse the correlation of ZIP1 and Caspase-3 expression in prostate adenocarcinoma on its grading as represented by Gleason Score. This was a cross-sectional, retrospective analytical study on 31 Formalin-fixed, paraffin-embedded tissue that meets inclusion criteria. The specimen was stained using the immune-histochemistry technique for ZIP1 and Caspase-3. Protein expression of each case was counted using ImageJ analysis. Gleason score was acquired as secondary data from the cases' reports. The correlation of their expression with respect to Gleason score was analysed with Pearson's correlation using SPSS 11.5. Results: Mean expression level of ZIP1 and Caspase-3 in prostate adenocarcinoma were 35% and 33%, respectively. There was a significantly positive correlation between ZIP1 and Caspase-3 expression (r = 0.379; p = 0.018). However, their correlation was stronger in intermediate-grade group (r = 0.73; p = 0.01) and the correlation was much weaker in high-grade group (r = 0.04; p = 0.48). Conclusions: There was a positive correlation between ZIP1 and Caspase-3 expression in prostate adenocarcinoma.
Sodium Benzoate is Associated with Salmonella typhi Resistant to Chloramphenicol Fajar, Jonny K; Puspitasari, Retno A; Dewi, Ariani R; Yahya, Arif; Anand, Jay R
Makara Journal of Health Research Vol. 20, No. 1
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Abstract

Background: There are many factors that govern growth and resistant of Salmonella typhi. A study had reported that the use of sodium benzoate caused antibiotic resistant. However, no study has directly evaluated the effect of sodium benzoate exposure on S. typhi sensitivity to chloramphenicol. The aim of this study was to evaluate the resistance or sensitivity of S. typhi to chloramphenicol after sodium benzoate exposure. Methods: The study was conducted in seven groups: three treatment groups (sodium benzoate insensitive S. typhi+8 µg/mL, 16 µg/mL, and 32 µg/mL of chloramphenicol), three positive control groups (sodium benzoate sensitive S. typhi+8 µg/mL, 16 µg/mL, and 32 µg/mL of chloramphenicol), and one negative control groups (sodium benzoate sensitive S. typhi+0 µg/mL of chloramphenicol). The effect of sodium benzoate exposure to S. typhi sensitivity to chloramphenicol was measured after 24 hours. Spearman test was used to analyzed this association. Results: In this study, we found that the average S. typhi growth in the treatment groups (A, B, C) was 445 CFU/mL, 385 CFU/mL, and 171 CFU/mL, respectively. While in the positive control group (D, E, F) was not obtained any S. typhi growth. Average S. typhi growth in the negative control group was 430 CFU/mL. Discussion: We found that sodium benzoate exposure inhibited S. typhi growth and affected S. typhi sensitivity to chloramphenicol (p < 0.05). In addition, we found that 32 µg/mL chloramphenicol had the highest mean difference value, so this showed that the dose 32 µg/mL of chloramphenicol had the best effectiveness of various treatment groups (p < 0.05). Conclusions: Sodium benzoate exposure can inhibit S. typhi growth and cause S. typhi resistant to chloramphenicol.
Radiographic Study of Developmental Alteration in Tooth Number among Children in IIUM Dental Clinic Sukmasari, Susi; Ismail, Nabilahuda; Azaldin, Nurul H; Ardini, Yunita D
Makara Journal of Health Research Vol. 20, No. 1
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Abstract

Background: The aim of this research was to determine the prevalence, distribution and association of developmental alteration in tooth number with gender, location, and presence of delayed eruption of tooth among children who attended to Student's polyclinic of IIUM Kuantan, Malaysia. Methods: A cross sectional study on total 727 Orthopanthomograms (OPGs) of 3-17 years old children from April 2009 to July 2012. OPGs with presence of alteration number were scrutinized to find out the prevalence, distribution and association. The data was analyzed comparing gender, location and presence of delayed eruption and tested using Chi-square test. Results: Out of 727 OPGs, 71 OPGs showed developmental alteration in tooth number. The prevalence was 98/1000 OPGs within 3 years. Among those 71 OPGs, the gender differences were: males (49.3%) and females (50.7%). Locations were found in maxilla (45.1%), mandible (40.8%) and both (14.0%). The finding of delayed eruption was (7.0%). There were significant differences between alteration in tooth number with location as (p < 0.05) while there were no significant differences with gender and delayed eruption as (p > 0.05). Conclusions: This study showed that hyperdontia was considerably lower than hypodontia. There was association between alteration in tooth number with location, but no association with gender and delayed eruption.
Examination of Telomerase Expression with Immuno-Hystochemistry Techniques on Some of Cancer Cells Purwaningsih, Endang; Suciati, Yulia; Widayanti, Etty
Makara Journal of Health Research Vol. 20, No. 1
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Abstract

Background: Cancer is a disease that gets serious attention in the medical world. This is due to the ever increasing number of patients and there has been no effective way to treat. Cancer cells have telomerase activity is relatively high compared to normal cells, so the cancer cells have the ability to continue to proliferate. Cancer cells undergo uncontrolled mitosis and have high telomerase activity compared to cells normal. Telomerase is an enzyme responsible for telomere length, a segment of DNA that is the tip of chromosomes in eukaryotic cells. Telomeres are associated with the process of ageing and carcinogenesis. The purpose of this study was to determine the expression of telomerase in some cells such as breast cancer, cervical cancer, and lung cancer. Methods: The research method is experimental studies in several cancer cell cultures in the form of cell line. Cancer cells used were: HeLa (cervical cancer), MCF7 and T47D (breast cancer), WiDr (lung cancer), and Raji (lymphoma) with culture medium RPMI, DMEM, and M199. Vero cells is used (fibroblast cells) as a control (normal cells). Expression of telomerase enzyme was measured by the Immunohystochemistry (IHC) method. Results: The results showed that the cancer cells have activity/higher telomerase expression were highly significant (p < 0.01) compared to normal cells (Vero cells). Similarly, the expression of telomerase in HeLa versus WiDr, WiDr versus T47D, T47D versus Raji, and Raji versus MCF7 also showed highly significant differences (p < 0.01). Telomerase expression between cancer cells that showed significant difference (HeLa cells versus Raji cells; HeLa cells versus MCF7 cell; T47D cells versus MCF7 cells) (p<0.05). No significant difference was found in the group of HeLa cells versus T47D, WiDr versus Raji cells, and WiDr versus MCF7. Conclusions: It was concluded, that the cancer cells have telomerase expression of specific and different from each other, depending on the type of cell. T47D breast cancer cells have telomerase expression of the highest, followed by cervical cancer cells (HeLa). Lung cancer cells (WiDr) with cell lymphoma (Raji) has almost the same expression and both have lower expression.
Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines Hamidon, Hanisuhana; Taher, Muhammad; Jaffri, Juliana M; Tg Zakaria, Tg M; Sulaiman, Wan MAW; Susanti, Deny; Ichwan, Solachuddin JA; Zakaria, Zainul A
Makara Journal of Health Research Vol. 20, No. 1
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Abstract

Background: Garcinia xanthochymus extract has been reported to have several pharmacological properties. This study was conducted to evaluate cytotoxic and anti-inflammatory activities of G. xanthochymus extracts on cell lines. Methods: The roots and stem barks of plant were extracted using maceration method with n-hexane, dichloromethane and methanol, successively. Cytotoxic activity of the extracts was tested against MCF-7 breast adenocarcinoma using MTT assay. Anti-inflammatory study was evaluated using RAW 264.7 mouse macrophage cells. The nitric oxide production in LPS-stimulated cells was measured using Griess reagent. Results: The results of cytotoxic and anti-inflammatory study showed that dichloromethane and n-hexane extracts of root and stem bark exhibited cytotoxic activity in dose-dependent manner. Meanwhile, for anti-inflammatory study, all root extracts together with stem bark dichloromethane and n-hexane extracts reduce NO production in LPS-stimulated cells in dose dependent manner. Conclusions: This finding indicated that G. xanthochymus extracts might become interesting candidate for treatment of cancer and inflammation.

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