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Tropical Medicine Journal
Published by Universitas Gadjah Mada
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
Arjuna Subject : -
Articles 62 Documents
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Distal Renal Tubular Acidosis (dRTA) Among Southeast Asian Ovalocytosis (SAO) Patients in Malaria Endemic Area of Sekotong, Lombok Island I Gusti Ayu Nyoman Danuyanti; Tasmini -; Ahmad Hamim Sadewa
Tropical Medicine Journal Vol 1, No 1 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/tmj.4568

Abstract

Introduction: Southeast Asian Ovalocytosis (SAO) is caused by 27 bp deletion of the band 3 protein gene in erythrocyte membrane and characterized by oval erythrocyte. The erythroid band 3 (AE1) gene isexpressed not only in erythrocyte membranes but also in the cell membrane of α-collecting renal tubular functions in the secretion of acid in renal tubules and HCO3 -/Cl- anion exchange. An alteration of the band 3 (AE1) gene functions in cell of α-collecting renal tubules reduces HCO3-/Cl- ion exchange resulting in decreased secretion of H+ ions and disturbances in the process of pickling urine as an indicator of distal renal tubular acidosis (dRTA).Objectives: To determine the occurrence of dRTA among Southeast Asian Ovalocytosis (SAO) patients in malaria endemic area of Sekotong, Lombok Island by analyzing expression of erythroid band 3 (AE1) gene.Methods: Ovalocytosis degree determined by oval erythrocytes morphology of 20%-100% in blood film. The 27 bp deletion of band 3 (AE1) gene was analyzed by polymerase chain reaction (PCR). An indicators of the dRTA was determined the pH of urine, levels of bicarbonate (HCO3-), potassium (K+) and chloride (Cl-) in the blood.Results: The degree of ovalocytosis more than 50% was found in 35,7% individuals and below 50% was found in 64,3% individuals. Percentage of 27 bp deletion of band 3 (AE1) gene among subject of ovalocytosispatients was 35,7% (n = 28) and frequency of the dRTA among SAO patients was 20% (n = 10). Individuals with SAO do not generally have dRTA. The presence of the two conditions in the same individuals suggests that there may be a common underlying molecular defect or genetic basis for SAO and dRTA.Conclusion: There is a relationship between the 27 bp deletion protein band 3 (AE1) gene with distal renal tubular acidosis (dRTA) among Southeast Asian Ovalocytosis (SAO) patients in Sekotong, Lombok Island, even the percentage is low.Keywords: distal renal tubular acidosis, Southeast Asian Ovalocytosis, Sekotong, (Lombok Island)
The Effect of Bacille Calmette-Guerin (BCG) on the Changes in Number and Functional Activities of Mononuclear Phagocytes in Malaria- infected Mice Model Supargiyono -; Mahardika Agus Wijayanti; Tri Ratnaningsih; Dian Nugraheni
Tropical Medicine Journal Vol 1, No 1 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/tmj.4569

Abstract

Introduction: Recent studies have indicated that Bacille Calmette-Guerin (BCG) vaccination may have benefi cial eff ect on the survival of infant living in malaria endemic area as well as of malaria-infected mice model. However, the impact of injection of BCG vaccine on the changes in number and functional activities of Mononuclear Phagocytes during malaria-infection in animal model is still poorly understood.Objectives: To evaluate the eff ect of BCG on the changes in number and functional activities of Mononuclear Phagocytes (MPs) during Plasmodium berghei infection.Methods: Two groups of 18 Swiss mice were used. The fi rst group was given 0.1 ml of BCG injection subcutaneously and the second was the control non-BCG group. One week after BCG injection, all mice in both groups were inoculated with 107 Plasmodium berghei infected erythrocytes. The parasitaemia were monitored daily and the number and functional activity of splenic and peritoneal macrophages were tested.Results: The parasites were detected in the blood of both groups on the fi rst day after infection. The parasitaemia in the control group grew slowly until day 3, followed by rapid increased up to 38.96% on day 9. Parasitemia of mouse which still alive on day 12 was 59.6%. The parasitaemia of BCG-injected mice were also increased at lower rate after day 3, and the mice still survive until day 15 after infection. The number of peritoneal macrophages from BCG-injected mice increased to a higher degree compared to the non-BCG injected mice. Moreover, the phagocytic activities of peritoneal macrophages in BCG injected group were increased higher up to twice (200%) of normal levels compared to the non-BCG control group which increase only up to 1,5 times (150%) of the normal levels.Conclusion: The injection of BCG on Plasmodium berghei infected Swiss mice resulted in the extension of survival of the mice until day 15, accompanied by higher increased in number of circulating blood, splenic and peritoneal MPs, and the phagocytic activities of peritoneal MPs up to 137% of the increased in non-BCG mice.Keywords: BCG vaccine, malaria, Plasmodium berghei, macrophages, phagocytosis.

Page 7 of 7 | Total Record : 62

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