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Polyphenols extracted from the Green Tea (Camellia sinensis) augments the protective immune responses in mice challanged with Salmonella typhimurium Ratnaningsih, Tri; Asmara, Widya; Sismindari, Sismindari
Medical Journal of Indonesia Vol 13, No 1 (2004): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.117 KB) | DOI: 10.13181/mji.v13i1.122

Abstract

Green tea is an aqueous infusion of dried unfermented leaves of Camellia sinensis. Numerous biological activities of green tea have been reported. The aqueous infusion and its polyphenolic substance are known for their activity as an antimutagenic,  antibacterial, hypocholesterolemic, antioxidant, and mutagenic of B lymphocyte. Studies have demonstrated that green tea polyphenols increase IL-12 production. Salmonella spp infection is an important public health problem in many countries. Cell-mediated immunity (CMI), especially T-cell help is important for protection against this infection. Recent evidence indicates that IL-12 is one such factor that plays a crucial role in the development of CMI. These studies were carired out to investigate the effect of green tea polyphenols to the immune cellulare in mice responses of mice during Salmonella typhimurium infection. The subject consisted of 36 female mice (Balb/C), 6-8 weeks old, divided into 3 groups. The first group was given 10 mg polyphenols/mouse, the second group was given 5 mg polyphenols/mouse, and the third group as the control. In day 31, all mice were infected with 108 CFU Salmonella typhimurium orally. On day 0, 3, 5, and 7 postinfection, 3 mice from each groups were sacrificed, the splenocytes were extracted and cultured to measure  the level of IFN-g in the supernatan and. The peritoneal macrophages were also extracted and cultured to measure the phagocytic activity. The level of IFN-g in splenocyte culture supernatant  increased during infection  in all groups, but the level of the experimental groups  were higher than in control group. The  percentage of phagocytic activity of peritoneal macrophages were higher in the experimental groups than in the control group. The increase of the phagocytic activities were seen corelate with the level of IFN-g supernatan splenocyte culture. (Med J Indones 2004; 13: 1-7)Keywords: polyphenols, green tea, macrophages, phagocytosis
The effects of excessive disodium ethylene diamine tetraacetic acid (Na2EDTA) anticoagulant concentration toward hematology profile and morphology of erythrocytes in peripheral blood examination Ratnaningsih, Tri; Sukorini, Usi; Gumilang, Rizki A.
Medical Journal of Indonesia Vol 15, No 3 (2006): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.098 KB) | DOI: 10.13181/mji.v15i3.233

Abstract

The purpose of this study is to know whether there are differences between hematology profile and morphology of erythrocytes of blood specimens which are prepared with excessive Na2EDTA anticoagulant in different concentration. This study was conducted in Faculty of Medicine Gadjah Mada University. The criteria of subject were male, age from 18 until 22 years old and healthy, ascertained from history taking and vital sign examination. Blood samples from 33 subjects were taken using vein puncture. Two millimeters blood was divided into 4 Na2EDTA-containing tubes. Before that, one drop of blood without Na2EDTA anticoagulant was used for making control blood film right after vein puncture. Each tubes contained different concentration of anticoagulant. The first tube contained Na2EDTA in standard concentration 2 mg/dl; the remaining tubes contained consecutively, 4 mg/dl, 6 mg/dl, and 8 mg/dl. Those samples were immediately examined using SYSMEX SE-9500 automatic analyzer for measuring erythrocytes hematological profile and were stained with Wright staining for morphological examination. These procedures were done before 20 minutes of vein puncture. There were significant decrease of RBC count, HGB, HCT, and MCHC and also significant increase of MCV and RDW between different concentrations of excessive Na2EDTA anticoagulant. MCH did not have significant result. Morphological examination showed significant morphological changes in the form of echinocytes and appearance of ghost cells in the sample treated with excessive Na2EDTA anticoagulant concentration. In conclusion, there are differences in hematological profile and morphology of erythrocytes among blood specimen which are prepared with excessive Na2EDTA anticoagulant in different concentration, except for MCH. Excessive Na2EDTA anticoagulant concentration will affect the blood specimen for peripheral blood examination of erythrocytes by interfering morphology and some of hematological parameters. (Med J Indones 2006; 15:157-64) Keywords: Na2EDTA, erythrocytes, hematology parameters, echinocytes, ghost cells
The effect of excessive disodium ethylene diamine tetraacetic acid (Na2EDTA) anticoagulant concentration on leukocytes profile in peripheral blood examination Sukorini, Usi; Ratnaningsih, Tri; Ikarumi, Diannisa
Medical Journal of Indonesia Vol 16, No 3 (2007): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.473 KB) | DOI: 10.13181/mji.v16i3.271

Abstract

Accuracy of leukocytes profile assessment is influenced by several pre analytical factors, among others, the anticoagulant concentration. EDTA is one of the most frequently used anticoagulant in peripheral blood examination. Several references stated that inappropriate concentration of EDTA anticoagulant in blood sample may affect the result of leukocytes profile in peripheral blood examination. The aim of this study was to evaluate whether there are differences among leukocytes profile in peripheral blood examination specimens, which were prepared with excessive Na2EDTA anticoagulant in different concentration. This study was conducted in Faculty of Medicine, Gadjah Mada University. Blood samples from 30 subjects were taken using vein puncture. Two millimeters blood was divided into 4 Na2EDTA-containing tubes. Before that, one drop of blood without Na2EDTA anticoagulant was used to make blood film right after vein puncture, as control. Each tubes contained different concentration of anticoagulant. The first tube contained Na2EDTA in standard concentration 2 mg/ml; the remaining tubes contained 4 mg/ml, 6 mg/ml, and 8 mg/ml respectively. These samples were immediately examined using SYSMEX SE-9500 automatic cell counter to measure the total and differential leukocytes count; and were stained with Wright staining for morphological examination under the microscope. These procedures were done before 20 minutes of vein puncture. There were significant decrement of total leukocytes count, absolute differential leukocytes count and monocyte percentage following excessive Na2EDTA administration. Neutrophil percentage was found to be relatively increased and the difference was significant. Lymphocyte, eosinophil and basophil percentages were not significantly different. Morphological examination showed significant increment in irregular cytoplasm margin, vacoulation and irregular nuclei lobes following excessive Na2EDTA administration. It is concluded that excessive concentration of Na2EDTA used in blood specimen preparation, will lead to changes in leukocytes profile as the concentration increased. Standard Na2EDTA anticoagulant concentration did not alter any leukocytes count and morphology, except for irregular cytoplasm margin and irregular nuclei lobes. (Med J Indones 2007; 16:168-75)Keywords: Na2EDTA, anticoagulant, leukocytes profile, leukocytes count, leukocytes morphology
MOTIVASI WANITA USIA REPRODUKSI SEHAT ( 20-35 TAHUN ) PADA PEMILIHAN KB IUD PASCA PLACENTA Ratnaningsih, Tri; Rosalia,
JURNAL KEPERAWATAN BINA SEHAT Vol 12, No 2 (2015): Juli-Desember 2015
Publisher : JURNAL KEPERAWATAN BINA SEHAT

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Abstract

KB (family planning) Post Placenta is supply to KB (family planning) for patient who has childbirth till the time as long as 42days. The woman’s reproduction health age (20-35 yearsold) is an reproduction woman which has organ. Fisiology and pshychology optimily have not reached to emotion and spirituation is adult enough as like this women has a good reproduction organ so make a pregnancy faster. Support from family and her husband is more important to shoose a good KB (family planning) specifically. IUD,s family planning (KB) Post Placenta. The purpose for this research is to know the motivation of health reproduction woman age (20-35). Kind ofthis research is descriptive research included by population 278 person following woman,s health reproduction age in Blimbing city. Sooko. Mojokerto on july 2015. The sampling technical is used to purposive sampling. The data was taken by used questionaire and next to scorrying with likest skala most of this result is showed that a good reproduction women age. Are 119 respondent who has motivation enought 76 person (63,3 percent). They need a motivation to choose KB accuratelly to thread cancel and spase for her pregnancy and look foor more information about KB for the one. Mother and ungood news. Hopefully most all the husband help their wife to give information and motivation to choose a good KB specific for IUD pasca placenta.Keyword : Motivation,Healthy Women Reproductive Age,IUDPostPlacentaKB (Family Planning)
GAMBARAN REMAJA PUTRI USIA 14 – 16 TAHUN TENTANGCARA MENANGANI NYERI HAID DI SMP TAMAN SISWAKOTA MOJOKERTO Ratnaningsih, Tri; Purwa Seven, Nilam
JURNAL KEPERAWATAN BINA SEHAT Vol 11, No 1 (2015): Januari-Juni 2015
Publisher : JURNAL KEPERAWATAN BINA SEHAT

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Abstract

Reproductive health is an important issue for teenagers. The earliest changes in young women was to start having periods which can lead to menstrual pain that can be debilitated and can indirectly impact on productivity and quality of life of young women. The purpose of the research to find a picture of young women aged 14-16 years about how to deal with menstrual pain in the Junior Student Park Mojokerto. Design research use descriptive research. The entire population of young women who has experienced a primary dysmenorrhoea and menstrual age of 14-16 years . They were 55 respondents to obtain a sample of 31 respondents were taken by purposive sampling. The data were taken used a questionnaire with Likert scale, then interpreted in the form of a frequency distribution table for the data public and to specific data. This research implementation in SMP Taman Siswa Mojokerto city in Okt 2014. This research used presentil data with cross tab data analisys. The results show that young women deal with painful menstruation are not exactly as many as 19 young women (61.3%), most of the respondents aged 16 years old with 14 girls (45.16%). Young women deal with painful menstruation is not exactly as many as 19 young women (61.3%). This is due to the lack of information they receive, so teens need to seek information from the mass media or ask the teacher / parent, and can attend seminars on dysmenorrhoea than positive information, if someone is getting a lot of knowledge to get the information more widely and more level of knowledge in dealing with menstrual pain appropriately.Keywords: dysmenorrhoea, how to deal with painful menstruation, young women
DAMPAK PEMBERIAN MAKANAN PENDAMPING AIR SUSU IBU (MP-ASI)  DINI PADA BAYI USIA 0-6 BULAN DI DESA SUMBERJATI Ratnaningsih, Tri; Anama, Riza
JURNAL KEPERAWATAN SEHAT Vol 12, No 02 (2015): JKS : JULI-DESEMBER 2015 Volume 12No. 02
Publisher : JURNAL KEPERAWATAN SEHAT

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Abstract

Food addition of breast milk (MP ASI) is a addition food given to babies after aged 6 months to 24 months. If babies get food in addition to breast milk too early it will increase the risk of diarhea diseases, allergies, weight gain / obesity, the frequency of drinking milk decreased. At the time of 0-6 months old baby exactly just need nutrition to breastfeeding, to obtain optimal growth and development of breastfeeding for 6 months is recommended. The research objective was to determine the impact of giving food addition early aged 0-6 months in Sumberjati village. The used method is descriptive. Population 21 people taken all with total sampling. The variable impact of giving food addition early. Data was collected using a checklist instrument. The data have been obtained and analyzed by process of editing, coding, scoring, tabulating further processed statistically with the presentation in the form of a frequency distribution table. The results showed most of the impact of giving food addition early in babies aged 0-6 months are weight gain / obesity as many as 12 respondent (37.5%). Provision of complementary foods in infants aged 0-6 months causing weight gain / obesity are not in accordance with age so that it can affect the growth and development of the babys physical and psychological. To alleviating the impact of giving food addition early, is precise information about the schedule of giving food addition and the impact of the giving food addition.Keywords: impact of giving food addition, babies aged 0-6 months
PENGARUH PEMBERIAN ALAT PERMAINAN EDUKATIF PADA ANAK USIA TODDLER (1-3 TAHUN) TERHADAP PERKEMBANGAN ANAK DI DESA MOJOWANGI  KECAMATAN  MOJOWARNO KABUPATEN JOMBANG  Ratnaningsih, Tri; Zainuri, Imam
JURNAL KEPERAWATAN SEHAT Vol 4, No 2 (2012): JKS : JANUARI- JUNI 2012 Volume 4 no. 01
Publisher : JURNAL KEPERAWATAN SEHAT

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Abstract

Currently the community is still found children who can not use age-appropriate games, toddler age children should have been able to use these games so that children can not develop according to his age. Thats why there are still many children who experience delays in the development. The purpose of this study to analyze the Effect of Tool on Childhood Educational Games Toddler (1-3 Years) Against Child Development in the Village District MojowangiMojowarnoJombang District. The method used in this study is an analytical study is an observational analytic, with a "Cross Sectional". Its population is around the toddler age children (1-3 years) in the Village District MojowangiMojowarnoJombang District. Samples in this study were children ages toddler (1-3 years) of 35 children, purposive sampling technique of sampling. Independent variables giving the game an educational tool, the dependent variable of child development. Data obtained by questionnaires and tests disseminate child development at the toddler age children (1-3 years). The results of this study indicate a significant effect by using the Spearman rank test with significance level (0.05) obtained the P value (0.000). These results indicate H0 is rejected, so there is the effect of giving tools to the development of educational games toddler age children (1-3 years). The results of data collection tools available educational games that are not rendered correctly by 16 children (45.71%), child development suspect 12 children (34.29%) and untestable 6 children (17.14%). This is because children are not introduced by means of educational games because of a lack of understanding about the importance of educational toys for child development, children should have educational games so that age-appropriate normal child development.Keywords: Delivery of Equipment Educational Games, Children Ages Toddler (1-3 years), Child Development
HUBUNGAN KETEPATAN PEMBERIAN MAKANAN PENDAMPINGASI DENGAN PERKEMBANGAN BAYI USIA 9-12BULANDI DESA WATESUMPAK KECAMATANTROWULAN MOJOKERTO Ratnaningsih, Tri; Murti Utami, Titis
JURNAL KEPERAWATAN SEHAT Vol 11, No 01 (2015): JKS : JANUARI-JUNI 2015 Volume 11No. 01
Publisher : JURNAL KEPERAWATAN SEHAT

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Abstract

Breast milk’s food supplement is a supplement which is given during 6-24 months old for completed babies’ nutrition that have not gotten from Breast milk. But there is mother gives Breast milk’s supplement early and lately who was not proper by time, type, frequency and quantity decided. Therefore, the gift of Breast milk’s food supplement affects the babies’ growth such as hard motoric, soft, social and independence. This research went for proving the relation of Breast milk’s food supplement precison with babies’ growth 9-12 months old in Watesumpak Trowulan Mojokerto. In research used analytical observational designed by case control. There whose 39 respondents are taken by total sampling. Data collection used Breast milk’s food supplement questionnaire and Pre Screening Development Questionnaire. This research used presentil with cross tab data analysis, so it can be concluded that the hypothesis of this research can be said that there tendency in relation of giving Breast milk’s food supplement accuracy with babies growth of 9-12 months old in Watesumpak Trowulan Mojokerto. Giving Breast milk’s food supplement well in time, type, frequency and quantity can be influenced by education, proffesion, earning, information, society and families’ supports. These support the process of babies’ growth because it is important progress in constructing the structure and the function of babies body so that the nutrition of babies with its growth must be balanced to reach the proper growth.Keywords:Breast milk’s supplement food, Growth, 9-12 months old babies
HUBUNGAN PERUBAHAN FISIK DENGAN Body Image REMAJA PUTRI DI PONDOK PESANTREN DARUL HIKMAH KEDUNGMALING SOOKO MOJOKERTO Ratnaningsih, Tri; Zakiyah, Nurul
JURNAL KEPERAWATAN SEHAT Vol 13, No 01 (2016): JKS : JANUARI-JUNI 2016 Volume 13No. 01
Publisher : JURNAL KEPERAWATAN SEHAT

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Abstract

The concrete change on female adolescents is physical change where the body develops fastly until reaching adults body shape that followed with the development of reproduction capacity. The physical change is closely related with adolescents’ condition. A good condition bumps into a good adolescents’ physical change. The role changing, physics and psychologist influence Body Image of somebody and Body Image has sturdy effect in physical estimation. The objective of this research is to analyze the correlation of physical change with Body Image of female adolescents in Pondok Pesantren Darul Hikmah Kedung Maling Sooko Mojokerto. Population on this research is whole female adolescents in Pondok Pesantren Darul Hikmah Kedung Maling Sooko Mojokerto, and the sample of this research are 34 respondents that taken by using purposive sampling technique. This research is analytic correlation research and using Cross Sectional Approach. The tabulation of data analysis used coding, scoring, tabulating, and tested by using Chi Square. Based on the result of research shows the significant correlation by using Chi Square test α=0.05 get that X2≥ X2tabel (25,972 > 3,81) means there is correlation between physical changing with body image of female adolescents in Pondok Pesantren Darul Hikmah Kedung Maling Sooko Mojokerto. The physical changing will influence on psychology, cognitive and social condition. The uncomfortable feeling on the body and misunderstanding on facing physical changing will show new behaviors on adolescents in behaving it.Keywords: Physical Changing, Body Image, Female Adolescents
DUKUNGAN SUAMI DALAM KEBERHASILAN IBU MEMBERIKAN ASI EKSKLUSIF (USIA 0-6 BULAN) DI DUSUN TALOK MOJOKERTO Ratnaningsih, Tri
JURNAL KEPERAWATAN SEHAT Vol 14, No 2 (2016): JKS : JULI-DESEMBER 2016 Volume 14No. 02
Publisher : JURNAL KEPERAWATAN SEHAT

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Abstract

The success rate of exclusive breastfeeding can be managed successfully with the encouragement of her husband to the mother to breastfeed the baby. Support husbands sometimes do not show up or husband does not seem to care. This is because he does not know the right way to support his wife. The purpose of this research was to determine the mothers husband support in the success of exclusive breastfeeding in Talok Hamlet Salen Village Bangsal Sub District Mojokerto District. This research was descriptive. The population in this research is all mothers with children aged 7-24 months in Talok Hamlet Salen Village Bangsal Sub District MojokertoDistrictas many as 30 people. Sampling technique of this research is total sampling. The variable in this research is the mothers husband support in the success of exclusive breastfeeding. Measuring instrument used was a questionnaire. The results suggest that most of the positive husbands support as many as 17 people (56.1%), and negative ones as many as 13 people (43.9%). Analysis of the data used is mean cut off point. The results of data analysis showed that the husband supports his wife to give breast milk to their babies until the age of 6 months. Husband provide positive support because it is already able to think about the good health of his wife and child so that his wife supports exclusive breastfeeding in mind the importance of exclusive breastfeeding for infants and the many benefits of exclusive breastfeeding.