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Journal of Global Pharma Technology
Published by Universitas Udayana
ISSN : 09758542     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and citations to articles published in JGPT. JGPT publishes original research work that contributes significantly to the scientific knowledge in pharmacy and pharmaceutical sciences- Pharmaceutics, Novel Drug Delivery, Pharmaceutical Technology, Cosmeticology, Biopharmaceutics and Pharmacokinetics, Pharmacognosy, Natural Product Research, Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmaceutical Analysis, Pharmacology, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics, Biotechnology and Applied Computer Technology. For this purpose we would like to ask you to contribute your excellent papers in pharmaceutical sciences.
Arjuna Subject : Kedokteran - Onkologi
Articles 2,439 Documents
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Sperm Quality in HIV-1 Seropositive Men under Antiretroviral Therapy at HIV Integrated Clinic of Dr. Ciptomangunkusumo Hospital, Jakarta, Indonesia Margaretha Napitupulu
Journal of Global Pharma Technology Volume 12 Issue 11 (2020) Nov. 2020
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Abstract

The effect of human immunodeficiency virus (HIV) infection on male fertility as assessed by sperm quality is still in controversy in various studies. On the other hand, HIV seropositive men who are stable under the influence of antiretroviral therapy (ARV) experienced an increase in quality and life expectancy that allow them to have offspring. This study aims to investigate the sperm quality of HIV seropositive men in Indonesia. In addition, this study also compared sperm quality in HIV seropositive men who were stable under the influence of ARV (case group) with HIV seronegative men (control group). Semen samples from HIV seropositive male patients at the HIV Integrated Clinic, Ciptomangunkusumo Hospital, Jakarta, Indonesia were collected and analyzed for sperm in the laboratory, including: volume, pH, concentration, motility, morphology and sperm vitality. Comparison of sperm quality between the two groups was analyzed using independent t-test and Kruskal-Wallis test. Significant decrease in sperm quality was shown in the concentration, percentage of progressive motility and sperm vitality (p
"Synthesis and Identification of (4, 5, 6 and 7) Membered Heterocyclic Derivatives from 5-nitrovanilline” Shaimaa Adnan Behget
Journal of Global Pharma Technology Volume 10 Issue 07: (2018) July 2018
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Abstract

AbstractThis work included synthesis new Schiff base (1-(3-((4-hydroxy-3-methoxy-5 nitrobenzylidene) amino) phenyl) ethan-1-one)(1)by reaction 5-nitrovanilline with3-aminoacetophenone in acid medium, then (1) react with 2-amino-6-methoxybenzothazole to get derivative(2). (2) React with (chloroacetylchloride, thioglycolic acid, glycine, alanine, sodium azide, phthalic anhydride, maleic anhydride) to give (β-lactam, thiazolidine, imidazolidine, tetrazole, oxazepine) derivatives. In other side5-nitrovanilline react with acetophenone derivative in alkaline medium to get3-(4-hydroxy-3-methoxy-5-nitrophenyl)-1-(4-hydroxyphenyl)prop-2-en-1-one which react with (hydrazine , phenyl hydrazine , 2,4-dinitro phenyl hydrazine , hydroxyl amine hydrochloride , urea , thiourea , ethyl cyanoacetate , malononitrile , guanidine hydrochloride ) to get ( pyrazol , isoxazole , oxazine , thiazine ,  pyridine , pyrimidine ) derivatives. The new synthesized compounds have been identified by their melting points, 1HNMR, 13CNMR and FT-IR spectra.Keywords: Schiff bases, Chalchone,β-lactam, Thiazolidine, imidazolidine, Tetrazole, oxazepine, Pyrazol, Isoxazole , Oxazine , Thiazine ,  Pyridine , Pyrimidine.
Genotypic Detection of Carbapenem-Resistant Escherichia Coli Producing NDM-1 Gene for the First Time in Baghdad/Iraq Nadheema Hammood Hussein
Journal of Global Pharma Technology .
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Abstract

For the first time, this study reports the presence of New Delhi Metallo-β-Lactamase gene (NDM-1 gene) among Carbapenem resistant Escherichia coli (E. coli) isolates in Baghdad / Iraq. Sixty six E. coli clinical isolates were collected from Baghdad Teaching Hospital/ Medical city in Baghdad. The isolates were obtained from different clinical specimens including urine, wound swabs, sputum and blood. All isolates were identified by Vitek-2 system and the susceptibility test to different antibiotics was also done by Vitek-2 system.  The results showed most isolates under the study were multidrug resistance and all 66 E. coli isolates had 100% resistance to Ampicillin. The most effective antibiotic against E. coli isolates was Imipenem followed by Amikacin. The results also showed that 5 (7.6%) isolates out of 66 E. coli were resist for Imipenem (Carbapenem resistant E. coli isolates).  By using conventional PCR, the blaNDM-1 gene was detected in 2 (40%) isolates, out of 5 Carbapenem resistant E. coli isolates and the3 (60%) isolates of Carbapenem resistant E. coli don’t harboring bla NDM-1 gene. Sequencing of (983 bp) blaNDM-1 amp icon was carried out by Macrogen DNA Sequencing and aligning of the obtained sequences with the reference strains in Gen Bank confirmed the correct identification of blaNDM-1 in Carbapenem resistant E. coli. The data sequencing revealed (100%) homology with reference strains in Gen Bank and the data of blaNDM-1 sequencing was submitted to the Gen Bank of the National Center for Biotechnology Information (NCBI) under the accession number (KY966040).Keywords: Escherichia coli, Carbapenem resistance and bla NDM-1 gene genes.
Venotonics and Anticoagulants-undisclosed Potential of Drug Treatment of Chronic Venous Insufficiency Olga Pashanova
Journal of Global Pharma Technology Volume 12 Issue 01
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Abstract

This work aims to conduct a comparative analysis of anticoagulant and venotonic therapy for different groups of patients with a diagnosis of acute venous venous thrombosis of the lower extremities. The tasks included the development of anticoagulants and venotonics combinations and dosages for treatment of a) alleviate an existing disease in a patient; b) disease prevention. Rivaroxaban showed the results of a higher recanalization level of the lower extremities veins in patients compared with warfarin. The maximum recanalization indicators of lower extremities veins, the blood flow velocity in them, and the severity of clinical symptoms were obtained for a group of patients taking a combination of rivaroxaban and diosmin.Keywords: Acute venous insufficiency; Venotonics; Anticoagulants; Vein recanalization; Warfarin; Rivaroxaban; Diosmin.
Investigation of the Mechanism of Action of New Hepatoprotective Herbal Drugs in the Experiment Larysa V. Iakovlieva
Journal of Global Pharma Technology Volume 10 Issue 08: (2018) August 2018
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Abstract

Introduction. Rational pharmacotherapy for liver disease remains one of the most urgent medical and social problems. The purpose of the study was to determine the mechanism of action of new hepatoprotectors of plant origin in the model of acute toxic lesions of the liver of rats (tetrachloromethane, 0.8 ml / 100 g body weight, once, intragastric). Materials & Methods. Animals were divided into 8 groups of 7 animals in each group: 1 group - intact control; group 2 - control pathology (CC14); 3-6 groups - animals that received capsules Hepafisan (108 mg / kg), Fitovenol (150 mg / kg) against the background of СС14; Granules Gepatrombin (900 mg / kg) and Polygerbagastrin (900 mg / kg), respectively; 7-8 groups - animals that were given drug comparisons capsules Silibor (100 mg / kg) and Gepabene  (88 mg / kg). The investigated preparations were administered to animals in the treatment-and-prophylactic mode: 1 hour before administration and 1 hour after tetrachloromethane administration and on the next day. On the 3rd day after the simulation of the pathology in the blood serum of animals were determined the activity of alanine, aspartate aminotransferase (AlAT and AsAT) of alkaline phosphatase (ALP), the content of circulating immune complexes, C-reactive protein (CRP), proinflammatory (interleukin 1, (IL1) and tumor necrosis factor (TFN-α) and anti-inflammatory cytokines (interleukin 10 (IL10), the content of total and direct bilirubin in the blood serum. The absolute mass of the liver was determined. The content of the secondary products of LPO-TBC-reactants was determined in blood serum and in the biological substrate (liver tissues) activity of superoxide dismutase (SOD) and catalase (CAT), pyruvic acid (Pyruvate), lactate in serum - activity of succinate dehydrogenase (SDG), the content of glycogen in the liver, the liver detoxification function was investigated using a functional test called "Thiopental sleep" (thiopental in dose 35 mg / kg, intraperitoneally). Research results. It has been established that Hepafisan, Fitovenol, Gepatropin and Polyherbagastrin stimulate of reparative and regenerative processes in a liver, eliminate inflammatory processes. Under the action of biologically active substances that are part of the plant, there is a normalization of the processes of phosphorylation, the restoration of the cytochrome P-450-dependent monooxygenase system. By enhancing the utilization of glucose, they contribute to the synthesis of glycogen and provide a high level of energy supply to cells, which is likely to stimulate synthetic processes and improve hepatobiliary system function.Conclusions. A crucial role in the mechanism of hepatoprotective action of investigated means plays antioxidant properties that reduce in the damaging effects of lipid peroxidation processes, which contributes to regeneration of hepatocytes and functional reactivation of the liver.Keywords: Model of acute toxic damage of the liver, Tetrachlorolmethane, New hepatoprotective plants preparations, Hepatoprotective action.
Congenital Pseudoarthrosis Tibia (CPT) performed Free Vascularized Fibular graft : A case report Muhammad Bayu Wicaksono
Journal of Global Pharma Technology Volume 11 Issue 04.
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Abstract

AbstractCongenital pseudarthrosis of the tibia (CPT) remains one of the most challenging problems confronting the orthopaedic surgeon. The operative results are frequently less than successful; many cases require several surgical procedures, and a significant number of them ending in amputation. The purpose of this study was to access the surgical results, complications, secondary procedures, and long-term results of free vascularized fibular graft (FVFG). We reported a case of a 5 years old boy complained unable to walk, was diagnosed as Congenital Pseudarthrosis of the left tibia. The patient had been doing several operation procedure, finally performed FVFG. After 5 months follow up, ORIF with intramedullary Kirschner wire provided stability, and acceptable alignment resulted in bony union. However, even achieving union of pseudarthrosis is not enough for the resolution of the disease. This is only half of the problem, the other half is to maintain union. Keywords : Congenital pseudoarthrosis tibia, Free Vascularized Fibular graft
Anti-Alopecia Activity and Compound Content of n-Hexane Fraction of Cyclea Barbata Leaves Resmi Mustarichie
Journal of Global Pharma Technology Volume 12 Issue 02 (2020) Feb. 2020
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Abstract

One of the plants empirically used by the Pasemah tribe in South Sumatra, Indonesia,  as a hair grower is the green cincau (Indonesian) leaf (Cyclea barbata Miers.). Objective: The aim of this study was to determine the anti-alopecia activity and its compound content from the n-hexane fraction of the C. barbata leaves. Methods: Green grass jelly (C. barbata Miers.) Extracted with 96% ethanol and fractionated with water, n-hexane and ethyl acetate. The concentration of ethanol extract was diluted to 5%, 10%, 15%, 20% and 25% and all fractions were tested on rabbits by the modified Tanaka method. All data were analyzed statistically by ANOVA. Results: Test results showed that the ethanol extract of green grass jelly leaves had the activity of stimulating hair growth starting at a concentration of 15%. It was found that the n-hexane fraction had the best activity compared to other fractions and positive control (minoxidil 2%).Further tests using IR and LC-MS spectrophotometers found that the n-hexane fraction contained 1,4-dimethyl-1,2,3,6-tetrahydro-8H-pyrrolo[2’,3’:3,4]azepino[2,1-b]quinazolin-8-one. Conclusions: Ethanol extract and hexane fraction at concentrations of 15% and above were found to have anti-alopecia activity. The compound contained from the hexane fraction 1,4-dimethyl-1,2,3,6-tetrahydro-8H-pyrrolo[2’,3’:3,4]azepino[2,1-b]quinazolin-8-one] (C22H18N3O) which was thought to cause properties hair growth.Keywords: Hair loss, Anti-alopecia, Green grass jelly, Cyclea barbata Miers,  1,4-dimethyl-1,2,3,6-tetrahydro-8H-pyrrolo[2’,3’:3,4]azepino[2,1-b]quinazolin-8-one.
Morphology-based Selection, Metabolic Profiling and Omics Technology Compared with the Classical Morphology-based Embryo Selection in In-Vitro Fertilization Ria Margiana
Journal of Global Pharma Technology Volume 9 Issue 11
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Abstract

Introduction: The medical industry is one of the dynamic industries with frequent innovations and discoveries, which in-vitro fertilization (IVF) technology being the recent change. IVF is an assisted reproductive technology that was invented to treat fertility and genetic problems associated with conception which involves implantation of good quality embryosin to uterus. The success of IVF depends majorly on the embryo selection, the age of the gestational carrier and the cause of infertility. In IVF, more than one embryo can be implanted in uterus, which may result in multiple pregnancies about 30% of embryo resulting from IVF treatments have abnormal chromosome, and this percentage may rise up to 60% with poor embryo selection. This has led to many studies in the last decade on possible methods to replace the conventional morphological evaluations or complement with them. This paper, therefore, aims to assess the performance of morphology-based selection, metabolic profiling, and omics technology in IVF procedure when compared with the modern selection methods. Method: This study provides a review of literatures on the assessment of the effectiveness of the traditional embryo transfer methods when compared with the modern methods. The review was based on Pub Med electronic database. Information on female patient, different intervention models, comparisons and study designs was also provided. Results: Out of the 58 studies that were evaluated, only ten were identified as eligible for the review because they provided substantial reports on morphology assessment, metabolic profiling, and omics technology. The selected articles proved to be significant since they provided a comparative analysis of the models and their outcomes.  This review identified that the modern methods had positive results as opposed to the traditional methods that had less positive results.  Conclusion: Modern methods have positive results due to their precision compared to morphological technique. The time lapse imaging technique has the highest accuracy, and it complements the morphological method by reducing chances of failure of implantation caused by exposing the embryo to conditions outside the incubator.  Results from the other modern method including metabolic profiling, omics technology, whether they had lower pregnancy rates and significant implantation failure or not. From the results, one can judge that morpho kinetics is the best model due to its high precision.  On the integration of the time-lapse technique with classical morphology assessment, there was no significant difference between the time-lapse technique and the morphology evaluation. Although morph kinetics provides high-quality results, the traditional methods are continually being used because they provide an observational assessment of the embryo cells. Also, the morphology-based method issued worldwide due to the lack of consensus on the benefits of the novel embryo transfer techniques.Keywords: Morphology-based selection, Metabolic   profiling, Omics technology, In-vitro fertilization
Administration of Topical Passion Fruit Extract Cream (Passiflora edulis) inhibits the Increase of MMP-1 Levels and Decreases the Amount of Collagen in Wistar Rats (Rattus norvegicus) Exposed to UV-B Rays Anak Agung Gde Putra Wiraguna
Journal of Global Pharma Technology Volume 10 Issue 12.
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Abstract

Introduction: Aging skin is most prevalent due to exposure to sunlight, especially UV-B rays. UV-B rays which stimulate the formation of free radicals are highly reactive molecules with unpaired electrons that can cause elevated levels of MMP-1, causing a decrease in the amount of skin collagen. This study was an experimental animal study with a post-test only control group design.  Method: A total of 36 Wistar rat were divided into 2 groups, each consisting of 18 Wistar rat, the control group was smeared with placebo cream and the treatment intervention group was smeared with passion fruit seed extract cream 100%, all groups were exposed to UV-B with a total dose of 840 mJ / cm ² for 4 weeks, then an excision was carried out for the examination of dermis collagen amount and level of MMP-1.  Result: The results of the comparative analysis of the two groups using independent t-test showed that there were significant differences between the two groups, the mean level of MMP-1 and the mean amount of collagen with p <0.05. The mean level of MMP-1 and the mean amount of collagen treatment in intervention group were 2.32% and 70.32%, and control group showed the mean level of MMP-1 and the average amount of collagen at 2.99% and 63.63% respectively.  Conclusion: the administration of passion fruit seed extract cream inhibited the increase of level of MMP-1 and the decrease of total collagen level in Wistar rat skin was exposed to UV-B.Keywords: Passion fruit, MMP-1, collagen, Skin.
Bladder Endometriosis that Invisible to Repeated Cystoscopies: A Case Report Ida Bagus Putra Adnyana
Journal of Global Pharma Technology Volume 11 Issue 6.
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Abstract

Background: Bladder endometriosis (BE) is a form of Deep Infiltrating Endometriosis (DIE) which affects approximately 1% of women with endometriosis. BE commonly presents with lower urinary tract symptoms such as frequency, dysuria, hematuria, and, less frequently, bladder pain and urgency. The diagnosis and treatment of this condition could be difficult and requires multidisciplinary care. Due to overall rare cases, BE might pose a challenge, both to diagnose and treat. This article aims to share an experience in order to increase awareness and insight about BE for practising gynaecologist and urologist. Case: A Thirty-one years old woman, nulligravida, complained of painful, bloody stained urine, which occurs during menstruation since married for the last 18 months. Previously patient had a regular menstruation cycle with moderate pain and normal menstrual volume and no history nor any specific symptoms of disease. Gynaecological examination showed no remarkable finding. Ultrasound scan (US) by gynaecologist showed a mass protruded from the posterior wall of bladder. Cystoscopic finding by a urologist showed an adenomatous reddish nodular mass, and TUR was conducted. Pathological examination showed a cystitic glandulare. After three months temporary improvement, the symptoms reoccur. The patient underwent US and CT scan, again showed a mass, and another two rounds of TUR were conducted, to remove all residual mass. After three surgeries with no significant improvement, a team of gynaecologist and urologist were set to conduct a laparoscopic partial cystectomy with cystoscopy guidance to achieve complete excision of the lesion. The modified technique, which results in symptoms-free recovery, will be described in detail. Conclusion: BE could be challenging both in diagnostic and treatment. Cystoscopy and TUR are not recommended to diagnose and manage the lesions. A multidisciplinary approach is needed, and concomitant Laparoscopy with cystoscopy for partial cystectomy might give a satisfying outcome.Keywords: Bladder Endometriosis, Deep Infiltrating Endometriosis, Cystoscopy, Laparoscopic partial cystectomy, Lower urinary tract symptoms.

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