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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 18 Documents

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Search results for , issue "Vol 34, No 1 (2016): Juni" : 18 Documents clear

Biokimia Haryanto, Aris
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.22204

Biokimia

Penambahan Boraks dalam Bakso dan Faktor Pendorong Penggunaannya Bagi Pedagang Bakso di Kota Bengkulu Siti Istiqomah; Mirnawati B Sudarwanto; Etih Sudarnika
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The research of using borax in meatballs which was undertaken in the Bengkulu City in 2011 showed that 10% from 100 samples of meatballs containing borax. Considering the dangers of borax for the consumer if consume continuously and increasing the number of meatball traders until 2013, it is necessary to study with more meatballs samples and widely area. The aim of the study was to detect the presence of borax in meatballs, borax content after heating, storability meatballs which containing borax and determine the factors that support the meatball traders to use borax. The study was designed using cross sectional study using two types of data, they were data of meatball sample test in the laboratory and interview data to meatball traders in the BengkuluCity. Total of 160 meatball samples from traders and five meatball samples from grinders were collected and examined with qualitative test using turmeric paper. The qualitative test showed that 165 samples (100%) takenfrom meatball traders and grinders didn't contain borax. Knowledge and attitudes level of traders were at a good level. There were significant relationship between education level with knowledge, information source fromtelevision with attitude, and knowledge with attitude of traders in Bengkulu City (p < 0,05).

Karakterisasi Antibodi Poliklonal terhadap Aflatoksin M1 Angriani Fusvita; Romsyah Maryam; Eko Sugeng Pribadi
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aim of the research was characterized the polyclonal antibody at AFM for diagnostic reagens on track 1 and measure the levels of AFM in milk. The series of experiments are given a polyclonal antibody testing 1 dimetilaminobenzidin (DAB) by dot blot immunoassay (DBIA) so it will look brown color display on the positive control sample; purification and estimation antibody of AFM concentration with ammonium sulfate; 1 dialysis; fractionation using HiTrap Protein A HP column, measured using a spectrophotometer at a wavelength of 280 nm; and characterized of IgG using SDS-PAGE. DBIA test results showed a typical reaction betweenantigen AFM1-BSA with AFM1-BSA antibody to rabbit serum in the form of brown dots after addition of DAB substrate. The results of spectrophotometric against rabbit serum fractionation showed the type of IgG heavy chain.

Identifikasi Listeria monocytogenes pada Susu Kambing di Kabupaten Purworejo Jawa Tengah Monika Danaparamitha Andriani; Trioso Purnawarman; Retno Damayanti; Syafril Daulay
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Listeria monocytogenes is a pathogenic Listeria species, especially for high-risk groups and it that can be transmitted through contaminated food. Goat milk produced by traditional milking process and storage has a high risk of contamination. The aim of this study was to identify the presence of L. monocytogenes in fresh goat milk in Puworejo regency, Central Java. This study used 60 samples of raw goat milk that were obtained from seven farms by disease detection sampling method. All of the used method in this research refer to Indonesian National Standard (SNI) ISO 11290-1: 2012 about Microbiology of food and feed for detection and enumeration of Listeria monocytogenes. A total of 60 samples of raw goat milk that used in this study were not contaminatedwith L. monocytogenes. Based on the results of this study, it can be concluded that all the samples of raw goat milk were free from L. monocytogenes and have fulfilled the Indonesian National Standard (SNI) ISO No. 7388: 2009 about Limit of Microbial Contamination in Food.

Detection of Edwardsiella tarda From African Catfish (Clarias garipienus) by Agar Gel Precipitation (AGP) Method in jambi Surya Amanu; Miftahul Fikar; Rudi Barmara
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

For the past few years, Edwardsiella tarda has become major problem in African catfish culture in Jambi. Detection by biochemical characteristic can lead to inaccurate result and there is a necessity to develop more specific and accurate method, one of which is Agar Gel Precipitation (AGP) method. Six samples each were collected from two African catfish farm located in District Sungai Gelam and Telanai Pura in Jambi, which was showed clinical signs of E. tarda outbreak with more than fifty percent mortality rate. Heat stable soluble antigen was prepared from 2 groups of pure culture isolated from sample for AGP test. Antiserum for test wells was antiserum of E. tarda (ATCC 15947) that have been produced by inoculating whole-cell antigen (heat-stable) and flagellar antigen (formalin-killed) in rabbit. For control positive, soluble antigen prepared from E. tarda (ATCC 15947), and control negative from Aeromonas hydophila (ATCC 35654) and Edwardsiella ictaluri (NCIMB 13272). Both antiserums were able to show positive reaction visible by the formation of specific precipitin lines between antiserum and antigen wells, and there was no precipitin reaction for negative control. In conclusion AGP method is a one of reliable technique to identify E. tarda.

Perkembangan Embrio dan Penentuan Jenis Kelamin DOC (Day-Old Chicken) Ayam Jawa Super Asmarani Kusumawati; Rina Febriany; Shella Hananti; Mufti Sartika Dewi; Ninik Istiyawati
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Super Javanese chicken is the result of a cross breeding between male domestic chicken and female layer chicken. . There has not been much research aboutSuper Javanese chicken embryonic development. This study attempts to provide information about embryonic development and sexing on Super Javanese chicken. This study used Super Javanese chicken fertile eggs from Java Farm, Maguwoharjo, Depok, Sleman. These eggs were incubated at temperature of 37o-38oC. Samples were observed with Neutral Red and Hematoxilin Eosine (HE) staining. The measurement of the Super Javanese chicken embryo were about length of the third finger, beak, and the length of the embryo's body. This observation obtained embryonic development of the super Javanese chicken spesifically. This concluded that Super Javanese chicken as a first generation from cross breeding has slower growth in embryonic development

Tingkat Kejadian Escherichia coli Penghasil Extended Spectrum Β-Lactamase yang Diisolasi dari Feses Broiler di Kota Bogor Cholilia Abadiatul Masruroh; Mirnawati Bachrum Sudarwanto; Hadri Latif
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The use of antibiotics in food-producing animals may encourage the occurence of bacterial resistance named Escherichia coli that produces Extended Spectrum β-lactamase (ESBL). Antibiotical resistance is an important issue in the animal health and human health. This study focused on the presence of ESBL-producing E.coli in chicken feces. The number of sample feces was 100. E. coli isolates were obtained from broiler chicken feces in Bogor. Isolates obtained were identified using API 20E kit. Confirmation of ESBL in this study used Double disc method using antibiotical disc namely cefpodoksim, ceftazidime and cefotaxime. The existence ofESBL-producing E. coli which isolated from the feces of broiler chickens in the city of Bogor is 25% (4/16).

Efek Diet Tinggi Kolesterol Terhadap Peningkatan Kolesterol Darah, Gambaran Histopatologi Hati, dan Bobot Badan Kelinci New Zealand White Jantan Bibi Ahmad Chahyanto; Rimbawan Rimbawan; Sri Anna Marliyati; Wiwin Winarsih
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study was conducted to analyze the high cholesterol diet effect of egg yolk powder toward the increase of blood cholesterol and body weight in male New Zealand White rabbit and learned their liver histophatology figure. The experimental studies with Completely Randomized Design (CRD) used 13 male New Zealand White rabbit aged 6 – 7 months and weight 2,9 – 4,0 kg. Rabbit were divided into 3 groups: group 0 (n=4) given the standard feed, group 1 (n=4) given high cholesterol diet from egg yolk powder, and group 2 (n=5) given high cholesterol diet from egg yolk powder and red palm oil mix. The intervention was implemented for 8 weeks after 4 weeks adaptation period. The results showed that intervention of high cholesterol diet from egg yolk powder significantly increased levels of serum cholesterol and fatty liver, but did not significantly affect body weight of male New Zealand White rabbit.

Deteksi Gen Penyandi Sifat Resistensi Metisilin, Penisilin dan Tetrasiklin pada Isolat Staphylococcus aureus Asal Susu Mastitis Subklinis Sapi Perah Fatkhanuddin Aziz; Fajar Budi Lestari; Sarah Nuraidah; Endah Purwati; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Detection of gene encoding resistance of bacteria could be used as an accurate method to determine resistance of Staphylococcus aureus which is causing mastitis in dairy cows to the several antibiotics. This research aimed to detect the gene encoding resistance of methicillin, penicillin and tetracycline from identified S. aureus. Sixty milk samples were collected from subclinical mastitis of cows from various dairy farming in Yogyakarta. Isolation and identification of S. aureus based on the culture, Gram staining and biochemical test. Phenotypes of S. aureus resistances against antibiotics were carried out by disc diffusion method, meanwhilespecies specific gene of S. aureus and the gene encoding methicillin, penicillin and tetracycline were confirmed by PCR method. The results showed 11 isolates representing of Methicillin Susceptible Staphylococcus aureus (MSSA) could be identified, wherein 5 isolates were harboring both of penicillin and tetracycline resistant genes respectively.

Prevalensi dan Serovar Penyebab Leptospirosis pada Domba di Kabupaten Kulon Progo Guntari Titik Mulyani; Eko Sulistyadi; Antoni Kirwanto; Haryadi Haryadi; Ambar Widuri; Tri Atmojo; Anis Pramundari
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Leptospirosis is a zoonotic disease, caused by Leptospira interrogans. A source of transmission in human leptospirosis are rodents, livestock, pets and wild animals. The prevalence of leptospirosis in cattle soon after theoutbreak of leptospirosis in humans that occurs in Kulon Progo district in 2011, reached 3.4% by the various serovar Leptospira. Breeding conditions of the people who still puts cattle and sheep in a single environment which enables transmission of leptospirosis in cattle. The purpose of this study was to determine the prevalence of leptospirosis and identify serovar caused of leptospirosis in sheep in Kulon Progo. A total of 60 sheep were done blood collection from the jugular vein 5 ml, serum was separated for leptospirosis examination with Microscopic Aglutination Test (MAT) which conducted at the Research Center for Veterinary Science, Bogor. Microscopic Aglutination Test carried out on various Leptospira serovar, namely: chterohaemorrhagiae, Javanica, Celledoni, Ballum, Pyogenes, Cynopeteri, Rachmati, Auatralis, Pomona, Canicola, Grippotyphosa, Bataviae, Hardjo, and Tarrasovi. Leptospirosis prevalence rate was calculated by dividing the result by the number of MAT positive samples examined. Serovar types that give a positive agglutination result was serovar that caused leptospirosis in sheep. The results showed that two samples were positive against antigen serovarIchterohaemorrhagiae. Based on these results can be concluded that the prevalence of leptospirosis in sheep in Kulon Progo district were 3.3%. The cause of leptospirosis in sheep in Kulon Progo was Leptospira interrogansIchterohaemorrhagiae serovar.

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