Etih Sudarnika
Divisi Kesehatan Masyarakat Veteriner, Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agatis Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia 16680

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Seroprevalensi dan Faktor Risiko Rabies pada Anjing di Daerah Endemis Kabupaten Sukabumi Ardilasunu Wicaksono; Abdul Zahid; Etih Sudarnika; Dordia Anindita Rotinsulu; Denny Widaya Lukman; Sri Murtini; Yusuf Ridwan
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 3 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.3.436

Abstract

Sukabumi District is reported to date as an endemic rabies area in West Java Province. In supporting the rabies control programs, the occurrence of rabies needs to be determined to decrease the number of cases. This research aims to determine the seroprevalence of rabies and identify the risk factors associated with rabies in the district. The research was conducted in Jampang Tengah and Cisolok sub-districts with positive rabies cases on dogs and humans. The blood samples were taken from 191 selected dogs using simple random sampling. Rabies antibody titer was measured using ELISA, and the risk factor was identified using selected dogs’ data classified into intrinsic and extrinsic characteristics. Data were analyzed using the chi-square test and the calculation of the odds ratio. The results showed that the seroprevalence of rabies was 7.9% (4.9-10.9%). The average antibody titer was 0.23 EU with a minimum value of 0.03 EU and a maximum of 0.96 EU. Thus, as high as 7.8% of the population showed rabies seropositivity from the unvaccinated dogs, while among the vaccinated dogs, only 8.0% of the population with rabies protective antibody titer. The significant risk factor of rabies seroprevalence is the typology of raising area (p = 0.003), revealing that the dogs raised in the coastal area had the odds of 5.8 (1.6-21.2) higher to get the rabies seropositivity than the dogs raised in the highland area. Keywords: antibody titre, dogs, rabies, risk factor, seroprevalence
Similarities of Antimalarial Resistance Genes in Plasmodium Falciparum Based on Ontology Dinar Munggaran Akhmad; Yeni Herdiyeni; Etih Sudarnika
TELKOMNIKA (Telecommunication Computing Electronics and Control) Vol 16, No 1: February 2018
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/telkomnika.v16i1.6898

Abstract

The finding of P. falciparum chloroquine resistance (pfcrt) and P. falciparum multidrug resistance 1(pfmdr1) gene in P. falciparum has become an obstacle in treating malaria. The polymorphism between the two genes may result in molecular functions, in cellular components, or in biological processes. The objective of this research is to find similarities between the two genes in 3 components; cellular components, molecular functions and biological processes, based on Gene Ontology. the similarity will be counted semantically by path length approach with Wang method. The range of similarity values is 0-1. After the similarity value examined; in Molecular Function the similarity is 1 due to the same drug transmembrane transporter activity, in Cellular Component is 0,714, the similarity only at the same vacuole food cells, and in Biological Processes is 1 due to the same proces in responding to drug. Therefore, this research proves both genes have similarities based on gene ontology.
Karakteristik, Pengetahuan, Sikap dan Praktik Petugas Karantina Hewan dalam Pengendalian Bruselosis di Sulawesi Selatan . Sumitro; Hadri Latif; Etih Sudarnika
Acta VETERINARIA Indonesiana Vol. 2 No. 2 (2014): Juli 2014
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (569.429 KB) | DOI: 10.29244/avi.2.2.62-69

Abstract

Praktik atau perilaku petugas karantina hewan dalam pengendalian bruselosis dipengaruhi oleh faktor internal berupa karakteristik individu yang bersifat khas dan dipengaruhi oleh faktor eksternal berupa lingkungan, sosial dan budaya. Tujuan penelitian ini adalah mengidentifikasi karakteristik individu petugas karantina hewan dan menganalisis pola hubungan karakteristik, pengetahuan, dansikap terhadap praktik petugas karantina hewan dalam pengendalian bruselosis di Sulawesi Selatan. Desain penelitian yang digunakan adalah cross sectional study. Metode pengumpulan data melalui wawancara dan pengamatan terhadap 51 orang petugas karantina hewan di dua Unit Pelaksana Teknis Badan Karantina Pertanian di Sulawesi Selatan. Pengumpulan data menggunakan kuisioner terstruktur, dan dianalisis menggunakan path analysis. Hasil studi menunjukkan bahwa karakteristik petugas karantina hewan sebagian besar berusia antara 30-45 tahun, telah bekerja sebagai PNS maupun bekerja di tempat yang sekarang kurang dari lima tahun, pendidikannya SMA/sederajat. Tidak semua petugas karantina hewan adalah pejabat fungsional dan mayoritas belum pernah mengikuti pelatihan terkait bruselosis. Terdapat hubungan yang nyata antara pendidikan dan pengetahuan, pengetahuan dan sikap, serta sikap dan praktik. Pendidikan formal berperan penting dalam terbentuknya pengetahuan, sikap, dan praktik petugas karantina hewan dalam pengendalian bruselosis. Sehingga upaya peningkatan pendidikan formal pada petugas karantina hewan perlu dilakukan.
Identifikasi Penambahan Daging Babi pada Pangan Berbahan Dasar Daging Sapi Menggunakan ELISA dan qPCR Diyan Cahyaningsari; Hadri Latif; Etih Sudarnika
Acta VETERINARIA Indonesiana Vol. 7 No. 2 (2019): Juli 2019
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (772.628 KB) | DOI: 10.29244/avi.7.2.17-25

Abstract

Penelitian ini bertujuan untuk mengidentifikasi dan menganalisis penambahan daging babi ternak dan babi hutan baik yang mentah (raw) maupun yang diolah (cooked) di dalam pangan asal hewan berbahan dasar daging sapi menggunakan metode uji enzyme-linked immunosorbent assay (ELISA)  dan real-time PCR (qPCR). Sebanyak 40 sampel yang terdiri dari 20 daging babi ternak dan 20 daging babi hutan dihomogenisasi dengan daging sapi dalam bentuk mentah maupun olahan (bakso). Konsentrasi setiap daging babi ternak dan babi hutan dalam bentuk mentah dan olahan bakso pada campuran daging sapi antara lain 2%, 1%, 0.5%, 0.25%, dan 0.125%. Hasil uji ELISA pada penelitian ini menunjukkan bahwa uji ini mampu mengidentifikasi adanya penambahan daging babi ternak dan babi hutan dalam pangan asal hewan baik dalam bentuk mentah maupun olahan bakso hingga konsentrasi 0.25%. Hasil uji t terhadap nilai optical density (OD) uji ELISA menunjukkan bahwa tidak terdapat perbedaan dalam mendeteksi spesies babi ternak dengan babi hutan (p≥0.05), tetapi terdapat perbedaan pada sampel bentuk mentah dengan bakso (p<0.05). Hasil uji qPCR mampu mengidentifikasi adanya penambahan daging babi ternak dan babi hutan dalam pangan asal hewan baik dalam bentuk mentah maupun olahan bakso hingga konsentrasi 0.125%. Hasil uji t  terhadap nilai cycle threshold (Ct) uji qPCR menunjukkan bahwa tidak terdapat perbedaan dalam mendeteksi spesies babi ternak dengan babi hutan (p≥0.05), tetapi terdapat perbedaan pada sampel bentuk mentah dengan bakso (p<0.05). Metode ELISA dan qPCR dapat dijadikan sebagai metode pengujian untuk mengidentifikasi pencampuran spesies yang tidak dikehendaki, khususnya daging babi pada pangan asal hewan berbahan dasar daging sapi yang beredar di masyarakat.
Risk Assessment of The Use of Colistin Sulfate In Broiler Due To Escherichia coli Resistance In Broiler Flocks Maria Fatima Palupi; Etih Sudarnika; I Wayan Teguh Wibawan; Huda Shalahudin Darusman; Hera Maheshwari
Acta VETERINARIA Indonesiana 2019: Special Issues
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (647.096 KB) | DOI: 10.29244/avi.0.0.14-23

Abstract

The risk assessment of antimicrobial resistance is very important to determine the risk of decreasing antimicrobial efficacy can be used as a basis for policymakers in allowing or prohibiting the use of an antimicrobial. This study aims to assess the risk of using colistin against E. coli resistance in the broiler flock. Risk assessment is carried out qualitatively using primary data, interviews, and secondary data. To obtain primary data various studies were carried out including monitoring the prevalence of colistin-resistant E. coli and mcr-1 also mcr-2 genes in broiler flocks, mcr-1 gene transfer from E. coli to Salmonella Enteritidis, mcr-1 gene sequencing, mutant selection windows of colistin against E. coli, and also multiresistant of E. coli colistin-resistant. Assessment of the risk of E. coli colistin-resistant in the broiler flocks through direct contact with live broiler flock environment with the resulting assessment is a medium risk with low uncertainty. Since colistin sulfate is very critically important for humans, the reduced use of colistin sulfate in animal production is necessary to reduce the risk of resistance. Reducing the use of colistin sulfate requires the collaboration of various sectors such as the government, veterinary drugs industries, farmers, and consumers.
Partial Budgeting of the Application of Teat-dipping to Control Mastitis in Small Farms Dikky Indrawan; Etih Sudarnika; Chaerul Basri; Abdul Zahid Ilyas; Nyayu Lathifah Tirdasari; Mirnawati Sudarwanto
Acta VETERINARIA Indonesiana 2019: Special Issues
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (595.361 KB) | DOI: 10.29244/avi.0.0.24-28

Abstract

Subclinical mastitis has a higher prevalence than clinical mastitis in many small farmers in Bogor city, and it could reach more than 80 percent. However, the application of teat-dipping could help small farms to control subclinical mastitis prevalence. The objective of this study was to measure the cost of teat-dipping application as subclinical mastitis control in small dairy farms. The partial budget was employed based on the experimental data collected in small dairy farms in Bogor. A number of cows were the basis of simulation for the mean of milk value and the application cost. The findings show an incentive for farmers to apply teat dipping in their farms. The incentive of teat dipping application could be improved to prevent sub-clinical mastitis infection and increase milk production.
Factors Influencing Farmers Participation in the Vaccination Program against Anthrax in Bogor District, Indonesia Chaerul Basri; Etih Sudarnika; S Dwidzuriputra
Acta VETERINARIA Indonesiana 2019: Special Issues
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (688.908 KB) | DOI: 10.29244/avi.0.0.29-34

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Bogor District is one of the endemic areas of anthrax cases in Indonesia. The mass vaccination campaign on livestock including goats and sheep needs to be done to prevent the spread of the disease. The willingness of farmers to participate is the main key to the success of this vaccination campaign. This study aimed to identify the factors that influence the willingness of goat and sheep farmers to participate in vaccination programs against anthrax in their farms. A total of 60 goat and sheep farmers were randomly selected from 3 villages located in the region with the highest incidence of cases in Babakan Madang Subdistrict. Data was collected through direct interviews using a structured questionnaire. Analysis to determine risk factors was carried out by chi square test and continued by calculating the value of relative risk (RR) to measure the magnitude of the influence of these factors. The results showed that the factors that influenced the farmers to be willing to participate in the anthrax vaccination program were history that had been directly counseled with RR values 2,844 (95% CI = 1,547-5,288) and history of having constrained to vaccinate their livestock with RR values 1,960 (95% CI = 1,203 - 3,193). Based on these findings it is recommended to increase farmer participation in mass vaccination programs against anthrax can be done through increasing communication, information and education activities and minimizing constraints for farmers to vaccinate their livestock.
Postmortem Changes in pH, Color, Drip Loss, and Non-Protein Nitrogen in Beef Liver and Lungs During Storage in Refrigerator Denny Lukman; Herwin Pisestyani; Hadri Latif; Etih Sudarnika; Mirnawati Bachrum
Acta VETERINARIA Indonesiana Vol. 8 No. 3 (2020): November 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.8.3.56-63

Abstract

Beef offal are consumed by people in some countries specifically in Asia. Beef liver and lungs are favorite food which are used as meat in traditional food. The objective of this study was to determine the postmortem changes in pH, color, drip loss, and non-protein nitrogen (NPN) content in beef liver and lungs during storage in refrigerator (3-4 ºC) until 5 d (120 h) after slaughter. The beef liver and lungs were collected from the abattoir and transported in cool box (<7 ºC) to the laboratory within 3 hours. The samples size of beef liver and lungs were 20 for each observation time. In the laboratory the beef liver and lungs were measured directly for pH value, color (L*, a*, and b*), drip loss, and NPN content at 4 h postmortem (pm) and afterwards every beef liver sample was sliced into 5 pieces of 100-120 g and stored in chiller of 3-4 ºC. The measurement of pH, color (L*, a*, and b* values), drip loss, and NPN content were conducted at 4 h, 24 h, 48 h, 72 h, 96 h, and 120 h postmortem. Data were analyzed descriptively and by comparing the 95% confidence interval of mean of each observation. The results showed that pH, color, drip loss, and NPN content in beef lungs were higher than the values in beef liver. The pH of beef liver and lungs declines until 96 h pm and 48 h pm, respectively. The L*, a*, and b* values of beef liver and lungs increased in general during storage. Drip loss and NPN in beef liver and lungs tended to increase significantly during storage. From this study the pH value and NPN can be used to determine the freshness of beef liver and lungs.
Prevalensi dan Faktor Risiko Infeksi Cacing Saluran Pencernaan pada Kuda Delman di Kota Bogor Hadzash Peppyrhanggasidhi Shatyaayyupranathasari; Etih Sudarnika; Yusuf Ridwan
Acta VETERINARIA Indonesiana Vol. 9 No. 2 (2021): Juli 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.9.2.87-96

Abstract

Kecacingan pada kuda merupakan penyakit yang dapat mempengaruhi kesehatan dan performa kuda termasuk pada kuda delman. Penelitian ini bertujuan menduga prevalensi dan mengidentifikasi faktor-faktor risiko kejadian infeksi cacing saluran pencernaan pada kuda delman di Kota Bogor. Metode penelitian ini yaitu studi cross-sectional, menggunakan dua jenis data berupa hasil pemeriksaan laboratorium sampel feses dan hasil wawancara terhadap kusir delman menggunakan kuesioner terstruktur. Data dianalisis untuk menentukan rataan derajat infeksi cacing dan proporsi kuda yang terinfeksi serta pendugaan nilai risiko relatif (RR). Hasil penelitian 20 sampel feses menunjukkan prevalensi Strongyle sebesar 60%. Berdasarkan hasil identifikasi larva, jenis Strongyle terdiri atas Cyathostominae 73% (Cyathostominae tipe A 61%, tipe G 6%, tipe C 4%, tipe F 1%, dan tipe H 1%), Strongylus vulgaris 22%, S. equinus 3%, serta S. edentatus 2%. Derajat infeksi Strongyle termasuk kategori infeksi ringan dengan jumlah 282.5 telur tiap gram tinja (TTGT). Faktor risiko yang mempunyai hubungan terhadap kejadian infeksi Strongyle yaitu umur kuda, kepadatan populasi, jenis kandang dan pakan kuda, lokasi dan cara pemotongan rumput, higiene kuda, serta sanitasi. Perbaikan manajemen pemeliharaan kuda perlu dilakukan oleh para pemilik kuda untuk menurunkan kejadian infeksi cacing saluran pencernaan.
Seroprevalensi Rabies Pascavaksinasi pada Populasi Anjing di Kawasan Endemik Rabies di Kabupaten Sukabumi Sri Murtini; Dordia Anindita Rotinsulu; Yusuf Ridwan; Denny Widaya Lukman; Abdul Zahid; Ardilasunu Wicaksono; Usamah Afiff; Asah Hilaliah; Risqika Akla Velayati; Etih Sudarnika; Koekoeh Santoso; Agus Wijaya
Acta VETERINARIA Indonesiana Vol. 10 No. 1 (2022): Maret 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.1.87-95

Abstract

Penelitian ini bertujuan untuk mengevaluasi hasil vaksinasi rabies dan membandingkan kenaikan titer antibodi yang terbentuk di Kecamatan Cisolok dan Jampang Tengah sebagai Kawasan endemik rabies Kabupaten Sukabumi dan mengasosiasikan titer antibodi yang terbentuk dengan umur, jenis kelamin, dan cara pemeliharaan anjing yang diperiksa . Sebanyak 211 anjing diamati pada studi ini. Sampel serum prevaksinasi dan pascavaksinasi diambil dan selanjutnya diuji dengan uji ELISA tidak langsung untuk mengetahui titer antibodinya. Data titer antibodi dari masing-masing sampel dianalisis secara deskriptif, sedangkan asosiasi umur, jenis kelamin, dan cara pemeliharaan dengan titer antibodi yang terbentuk pascavaksinasi dihitung menggunakan uji chi-square. Hasil pengujian menunjukkan seroprevalensi rabies di Kecamatan Cisolok dan Jampang Tengah sebelum vaksinasi adalah 17,3% dan 2,6% dengan titer 0,58 EU/mL dan 0,55 EU/mL, sedangkan seroprevalensi setelah vaksinasi menjadi 62,5% dan 93% dengan titer 1,29 EU/mL dan 1,33 EU/mL. Variabel umur, jenis kelamin, dan cara pemeliharaan tidak berasosiasi nyata dengan peningkatan titer antibodi rabies setelah vaksinasi. Hasil studi menyimpulkan bahwa seroprevalensi rabies di kawasan endemik rabies di Kabupaten Sukabumi meningkat setelah dilakukan vaksinasi dari sebelumnya 11% menjadi 75,8%, dengan nilai peningkatan titer (Δ titer) sebesar 1,09 EU/mL.
Co-Authors . Sumitro Abdul Zahid Adi Winarto Afiff , Usamah Agus Wijaya Amiruddin Saleh Andi Eka Putra Andi Eka Putra Andrijanto Hauferson Angi Anggraini, Raden Isma Annisa Madyanti Geminastiti Parampasi Ardilasunu Wicaksono Asah Hilaliah Asep Rakhmat ASEP SAEFUDDIN Atsmarina Widyadhari Bambang Sumiarto Bambang Sumiarto Chaerul Basri Citra Noviana Cut Desna Apriana Danang Dwi Cahyadi Daowen Zhang Denny Lukman Denny Widaya Lukman Denny Widya Lukman Dinar Munggaran Akhmad Diyan Cahyaningsari Dwida Agustina Suherman Edi Sukmawinata Ernes Andhesfa Evie Setyani F .X. Koesharto Fitri Hidayati Hadri Latif Hadzash Peppyrhanggasidhi Shatyaayyupranathasari HERA MAHESHWARI Heris Kustiningsih Heru Setijanto Herwin Pisestyani Huda Salahuddin Darusman Huda Shalahudin Darusman I wayan Teguh Wibawan Iccha Pradipta Patsiwi Isrok Malikus Sufi Isrok Malikus Sufi Jodi Vanden Eng Karin katina Hasya Katyusa Nandini Dyah Pitaloka koekoeh santoso Lis Rosmanah Lymbran Tina M. Gumilang Pramuwidyatama Manuel da Costa Mardi Fadillah Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Mazdani Ulfah Daulay Mirnawati B Sudarwanto Mirnawati B Sudarwanto Mirnawati B Sudarwanto1 Mirnawati B. Sudarwanto MIRNAWATI SUDARWANTO Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Muhammad Pauzi Lubis Mujiatun Mujiatun Murniati Murniati Nararya Adinata Nisa’, Chairun Nugraha, Arifin Budiman Nuraini , Henny Nurhidayat Nyayu Lathifah Tirdasari Okti Nadia Poetri Prima Mei Widiyanti Prima Mei Widiyanti R. Dikky Indrawan Rachmi Ramadhanita Raden Dikky Indrawan Raphaella Widiastuti raphaella widiastuti Retno Damajanti Soejoedono Retno Damayanti Soejoedono Risma Juniarti Silitonga Risqika Akla Velayati Rita Kusriastuti Ronald Tarigan Rotinsulu, Dordia Anindita Roza Azizah Primatika Roza Azizah Primatika S Dwidzuriputra Santi Lestari Satriyo Setyo Utomo Saukhan, Falih Prenata Savitri Novelina Septa Walyani Siti Istiqomah sri murtini . Srihadi Agungpriyono Sudarwanto, Mirnawati Bachrum Sunandar . Supratikno Susan Maphilindawati Noor Susi Soviana Taufiqqurrahman, Mas Trioso Purnawarman Umi Cahyaningsih Upik Kesumawati Hadi Uus Saepuloh Wasmen Manalu Widagdo Sri Nugroho widiastuti, raphaella Widiyanti, Prima Mei Widyadhari, Atsmarina William A. Hawley Yusuf Ridwan Zudanang .