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Jurnal Sain Veteriner
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Arjuna Subject : -
Articles 18 Documents
Search results for , issue "Vol 34, No 2 (2016): Desember" : 18 Documents clear
Kesembuhan Skin Flap H-Plasty dan Linear Closure untuk Penutupan Luka Area Lateral Thoraks Erwin Erwin; Deni Noviana; Gunanti Gunanti; I Gusti Agung Ngurah Arphan Eka Putra
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6815.375 KB) | DOI: 10.22146/jsv.27559

Abstract

Skin flap is one of the techniques use for large wound closure. This study were aimed to determine the rate of cure of skin flap H-plasty and linear closure based on subjective and objective clinical observation. Six male local cats at 1-2 years old, weighing 3-4 kg, were divided into two treatment groups. All cats were made 3 x 3 cm wound in the thoracic area for skin graft. Thoracic defects were treated with skin flap techniques H-plasty (group I) and linear closure (group II). Subjective observations was performed on days 3, 6, 9 and 12 after skin flap which was covering skin discoloration, pain response, time hair growth as well a bleeding tests that performed on the 18th day after the skin flap. Objective observation was made on day 18 by administration of 0.9% NaCland observed the absorption time, while the onset of drug effect was noticed by pupillary reflex observation after 0.2 ml adrenaline injection under the skin flap. The observation on day 9 showed that skin flap linear closure wasbetter than skin flap H-plasty, which was characterized by similar skin color with surrounding skin, reduced pain response and rapid hair growth. Bleeding blood test was showed a glowing red colored and immediately came outafter incision in both groups. The absorption time of 0.9% NaCl and the effects of adrenaline were faster in thegroup skin flap linear closure. Skin flap H-plasty and linear closure techniques can be applied to repair woundsin the thoracic area of cat skin, however, the linear closure flap of skin was healing faster than skin flap H-plasty.
Konsumsi, Kecernaan Nutrien, Perubahan Berat Badan dan Status Fisiologis Kambing Bligon Jantan dengan Pembatasan Pakan Bambang Suwignyo; Ulil Amri Wijaya; Rieska Indriani; Asih Kurniawati; Irkham Widiyono; Sarmin Sarmin
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6704.108 KB) | DOI: 10.22146/jsv.27560

Abstract

This study were aimed to determine the dry matter and organic matter intake, nutrients digestibility, body weight gain and physiological status of male Bligon goats treated with feed restriction. Six Bligon goats average age of 12 months and an average body weight of 23.6 kg were fed 40% of peanut raughages (rendeng) and 60% concentrate. Goats were divided into two (2) treatment groups. Control goat treatment were fed diets based on dry matter (DM) requirements 3.5% of body weight, and feed restriction goat treatment were fed diets with 50% reduction of DM requirement. Feed restriction was conducted for 35 days. The variables measured were dry matter (DM) and organic matter (OM) intake, total digestibility nutrient (TDN) intake, body weigh gain, dry matter (DM) and organic matter (OM) digestibility, rectal temperature, respiration frequency, and heart rate. Data were analyzed by independent samples T-test. The results showed that the feed restriction significantly decreasedconsumption of DM (676.07 ± 14.76 into 372.30 ± 53.08) and OM (639.38 ± 15.89 into 349.88 ± 49.35), consumption of TDN (461.60 ± 10.23 into 253.99 ± 36.17), DM digestibility (74.39 ± 1.60 into 71.27 ± 0.89) and OM digestibility (79.42 ± 1.54 into 76.34 ± 0.24), but had no significant effect on body  temperature, respiration frequency and heart rate (pulsus) of male Bligon goat. Value of ADG was 55.24 g/day (P0) then increased to 131.43 g/day when the feed was filled back. A feed restriction up to 50% decreased goat production performance, but did not interfere with the health status of livestock (can be indicated from physiological status of goat still in the normal range).
MPPA (Most Probable Producing Ability) Estimation of Kebumen Ongole Crossbred Cattle based on Offsprings Weaning Weight Sumadi Sumadi; Nono Ngadiyono; Diah Tri Widayati; Cuk Tri Noviandi; Akhmad Fathoni; Mukhamad Khusnudin
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6712.99 KB) | DOI: 10.22146/jsv.27561

Abstract

Tujuan dari penelitian ini yaitu untuk menghitung nilai MPPA induk pada sapi PO Kebumen. Penelitian ini telah dilakukan pada bulan Juli sampai dengan Oktober 2015 di wilayah Urut Sewu, Kebumen. Materi penelitian yang digunakan adalah data recording tiga tahun terakhir (2013-2015) terdiri dari 41 ekor pejantan, 51 induk dan 244 pedet. Data berat sapih anak sebelumnya telah dikoreksi berdasarkan umur induk, jenis kelamin dan umur penyapihan 120 hari. Ripitabilitas dihitung menggunakan metode korelasi antarkelas. Nilai MPPA induk dihitung berdasarkan nilai ripitabilitas berat sapih keturunan. Hasil penelitian menunjukkan bahwa nilai ripitabilitas berat sapih anak termasuk dalam kategori tinggi (0.32 ± 0.15). Nilai MPPA induk sapi PO Kebumen kemudian dihitung menggunakan nilai tersebut dan juga rata-rata performan populasi (83.45 kg). Dihasilkan 10 besar induk sapi PO Kebumen dengan nilai MPPA tertinggi. Nilai MPPA kemudian diurutkan dari nilai tertinggi ke terendah. Nilai MPPA tertinggi dimiliki oleh SAJ0315078 (110.69) dan terendah dimiliki oleh TA1112020 (97.20). Hasil dari penelitian ini kemudian dapat digunakan sebagai dasar dalam seleksi induk sapi PO Kebumen di dalam populasinya.
Kemampuan Fertilisasi Spermatozoa Sexing dan Perkembangan Awal Embrio Secara In Vitro pada Sapi Alvien Nur Aini; Mohamad Agus Setiadi; Ni Wayan Kurniani Karja
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (659.562 KB) | DOI: 10.22146/jsv.27562

Abstract

The aim of the present study was to investigate the fertilization ability of bovine oocytes and early bovine embryonic development in vitro, fertilized by frozen X and Y sperm separated by bovine serum albumin (BSA) gradient column. Oocytes were collected from slaughter house ovarian by flushing and slicing technique. Oocytes were than maturated in tissue culture medium (TCM) 199 supplemented with 10 IU/ml pregnant mare’s serum gonadotropin (PMSG), 10 IU/ml human chorionic gonadotropin (hCG) and 10% fetal bovine serum (FBS) for 24 h in 5% CO2 incubator 39oC. Oocytes then fertilized with three kind of different frozen spermatozoa (X,Y and unsexing spermatozoa as control) for 14 h with final concentration 2x106 spermatozoa/mL. Embryos were cultured insynthetic oviductal fluid (SOF) supplemented with essential and non essential amino acid and 0.3% bovine serum albumin (BSA) for 96 h. Results of the experiments revealed that there was no significant difference (P>0.05) in thefertilization ability (49.17%; 51.40%; 53.42%) for X, Y and control group, respectively. No significant difference (P>0.05) in the number of embryos development (47.77%; 48.25%; 54.43%) for X, Y and control group, respectively. Furthermore, only small number of embryos could pass development blockade (23.80%; 26.08%; 23.61%) for X, Y and control spermatozoa with statistically no significant difference (P>0.05). It is concluded that sexed spermatozoa separated by BSA gradient column had comparable fertilization ability with unsexing spermatozoa and had ability to supported early embryonic development.
Efektivitas Ekstrak Biji Kapas (Gossypium hirsutum L.) terhadap Jumlah dan Viabilitas Embrio Mencit (Mus musculus L.) Nofri Zayani; Iman Supriatna; Mohamad Agus Setiadi
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (816.406 KB) | DOI: 10.22146/jsv.27563

Abstract

The cottonseed (Gossypium hirsutum L.) contained gossypol as antifertility agent. The effect of cottonseed extract treatment could be decrease and impaired follicles development were accompanied by oocytesdamage. Damage of oocytes resulted reduction of number and viability of embryos. The objective of this study was to evaluate the effectiveness of cottonseed extract (Gossypium hirsutum L.) on the number and viability ofmice embryo (Mus musculus L.). Doses of the cottonseed extract were used consists of 0 (control), 1.5; 2.1; and 2.7 g/kg of body weight (BW) for 24 days via the oral route. This research used 24 animals healthy of femaleDDY mice 14-15 weeks old and 30-35 g BW. Embryos were collected at day 4 of pregnancy by flushing the utery cornua. The collected embryos were cultured in vitro for 48 hours in modified phosphate buffered saline (PBS) supplemented with 10% fetal bovine serum (FBS) culture medium according to the stage of its development to observe the viability of embryos. The result showed that the cottonseed extract with doses 1.5; 2.1; and 2.7 g/kg of body weight (BW) made the number of embryos which collected in D4 of pregnancy significantly lowerthan control (P<0.05). Data from embryos culture in vitro for 48 hours decreased embryos number (P<0.05) that developed in to the expanded and hatched blastocysts. At 2.7 g/kg BW, embryos only can develop to theblastocysts stage. Retardation (4-8 cells) and degeneration embryos did not develop in culture
Isolasi dan Identifikasi Enterobacter sakazakii pada Susu Mentah dan Produk Susu Segar di Daerah Istimewa Yogyakarta Tri Yahya Budiarso; Hutri Catur Sad Winarni
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.978 KB) | DOI: 10.22146/jsv.27565

Abstract

Enterobacter sakazakii is an opportunistic pathogen that causes infections such as necrotizing enterocolitis, bacteremia, and meningitis which, in many cases, have been associated with the consumption of powdered infant formula. Raw milk has been considered to be a potential source of contamination. The aim ofthis study was to detect and identify the presence of E. sakazakii in raw milk from local farms and fresh dairy products sold by street vendors and cafés in the Special Region of Yogyakarta. A total of 80 milk samples were cultured in Enterobacterriaceae Enrichment Broth. The cell cultures were then enumerated on Tryptone Soya Agar and Chromocult Coliform Agar. For the isolation of suspect colonies, Chromogenic Druggan-Forsythe-Iversen (DFI) Agar was used. Presumptive colonies of E.sakazakii were characterized biochemically using API20E and molecularly through the amplification of the 16S rRNA gene using primer pairs 16_SUNI-L/Saka-2b and ESA-1/16_SUNI-R. Based on the α-glucosidase test of DFI Agar, only 9 isolates out of the 80 samples wereidentified as suspected E.sakazakii. Biochemical and molecular identification suggested that 5 isolates resulted positive for E.sakazakii, while the other 4 were Panthoea spp1.
Kinerja Anak Kambing Bligon Setelah Introduksi Pejantan Unggul di Kelompok Ternak Purwo Manunggal, Gunungkidul Tri Satya Mastuti Widi; Endang Baliarti; Febri Ariyanti; Nono Ngadiono; I Gede Suparta Budisatria; Panjono Panjono; Muhamad Danang Eko Yulianto
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.751 KB) | DOI: 10.22146/jsv.27568

Abstract

Goats have become increasingly popular among smallholder mixed-crop-livestock farmers, such as in Gunungkidul District. Farmers in this district have good access to the market. However, farmers keep local breed,Bligon, without any improvement within breed. This study was started by introducing superior bucks to local does and aimed to evaluate the productivity of their progenies. Nineteenth kids, resulted from mating of local does andlocal bucks and 28 kids, resulted from mating of local does and superior Ettawa crossbred bucks were used in this study. Birth and weaning weights, average daily gain and body size of pre-weaning kids were measured. Birth andweaning weights of pre-weaning crossbred kids tended to be higher than local those of local kids but not significantly different. The average daily gain of crossbred kids were significantly higher than local kids. Body sizes interms of girth of chest (GC), height at the wither (HW) and length of the body (LB) of crossbreed kids were significantly higher than those of local kids. Productivity of pre-weaning kids in the preliminary phase of the introductionof superior bucks were highly varied. Continuous improvement of breeding system is then needed. Introduction of superior bucks has to be supported by good keeping management.
Efek Protektif Andrografolid terhadap Kejadian Kardiotoksisitas Pasca Aplikasi Doksorubisin pada Tikus Sri Wahyuni Salam; Agus Setiyono; Vetnizah Juniantito
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1133.57 KB) | DOI: 10.22146/jsv.27569

Abstract

Cardiotoxicity is one of the important side effects of doxorubicin, an anthracycline antibiotic and chemotherapeutic drug. The aim of this study was to explore the potential protective effect of andrographolide (Andro), an anti-inflammatory and anti-oxidant agents, against cardiotoxicity induced by doxorubicin (DXR). Thirty Sprague Dawley rats (80-100 g) were divided into four groups: (a) Control (b) DXR (4 mg/kg intraperitoneally (IP) were made weekly for 4 weeks), (c) DXR+Andro20 (low dose andro; 20 mg/kg IP weremade daily for 4 weeks, 24 h after DXR), (d) DXR+Andro100 (high dose andro; 100 mg/kg IP were made daily for 4 weeks, 24 h after DXR). Furthermore, at the end of experimental period, all rats were euthanized and hearts were removed for hispatological analyses. Hematoxylin-eosin (HE) and Masson Trichrome (MT) staining were used to observe the histomorphological alterations and fibrosis of hearts, respectively. Our results showed that andrographolide treatment (20 mg/kg) augmented the detrimental effects of DXR such as decreased body weight and heart weight, as compared with those in DXR-treated rats. Histopathologically, heart tissue from control group showed compact myocardial architecture without any noticeable lesions. Histopathological analysis fromDXR group showed severe inflammation and fibrosis, whereas DXR+Andro20 group showed almost normal heart morphology. Andrographolide at a dosage of 100 mg/kg did not show protective effects against doxorubicin,and even aggravated myocardial inflammation, as compared with DXR-treated rats. These results indicate that low dose of andrographolide compromised doxorubicin-induced decreased body weight, heart inflammation, andfibrosis.

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