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Kuntaman
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Journal of Clinical Microbiology and Infectious Diseases
Published by Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia
ISSN : -     EISSN : 28089405     DOI : https://doi.org/10.51559/jcmid
Core Subject : Science,
Immunology & microbiology
Journal of Clinical Microbiology and Infectious Diseases; peer-reviewed journal aiming to communicate high-quality research articles, reviews, and general articles in the field. JCMID publishes articles that encompass basic research/clinical studies related to microbiology and infectious disease. The Journal aims to bridge and integrate the intellectual, methodological, and substantive diversity of medical scholarship and encourage a vigorous dialogue between medical scholars and practitioners.
Arjuna Subject : Kedokteran - Mikrobiologi (Medis)
Articles 5 Documents
 1 
Search results for , issue "Vol. 3 No. 2 (2023): Available online: December 2023" : 5 Documents clear
The effect of low-electrical voltage as a method to eradicate Acinetobacter baumannii bacteria Putri, Naomi Lesmana; Koendhori, Eko Budi; Susilo, Imam; Tambunan, Betty Agustina
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 2 (2023): Available online: December 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i2.20

Abstract

Introduction: Acinetobacter baumannii is one of the bacteria in critical priority according to WHO in 2017 and one of the causes of nosocomial infections in the world. These bacteria usually become resistant to antibiotics (Multi Drug Resistant Organism, MDRO), infect the bloodstream, and cause pneumonia. This study aimed to examine the effect of low-voltage electricity as a method to kill Acinetobacter baumannii by using a different electric current. Methods: This study is experimental research in the Microbiology Laboratory of RSAB Harapan Kita. Experiments were carried out using a solution of Acinetobacter baumannii non-MDRO and MDRO bacteria with a parallel design device opposite to the GPS 3030D power supply with a power of 90 W. Each tube was fitted with a 12 cm stainless steel rod conductor with a diameter of 3 mm and delivered amperage 1 mA, 2 mA, 5 mA, and 10 mA and 0.5V with each amperage performed 5 times and measured using DensiCHEK. Result: From 20 Acinetobacter baumannii non-MDRO experiments and 20 MDRO experiments, it was found that from 1 mA, 2 mA, 5 mA, and 10 mA with monitoring time of 30 minutes, 2 hours, and 4 hours, the results of bacterial eradication by DC stimulation with 5 mA and the most optimal time is 30 minutes according to what has been applied. Conclusion: Using an electric current of 5 mA and a duration of 30 minutes can reduce Acinetobacter baumannii MDRO and non-MDRO bacteria with more optimal results in the non-MDRO group.
Effect of oral administration of in silico epitope-based SARS-CoV-2 virus with ISCOM adjuvants on increasing the number of NK cells and serum IgG in mice Irma Nur Sukmawati; Meike Tiya Kusuma; Khoirul Anam; Sumarno Retro Prawiro
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 2 (2023): Available online: December 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i2.34

Abstract

Background: Vaccines are one of the best solutions to deal with the COVID-19 pandemic. Epitope vaccines can be searched in silico. The selection of in silico epitope-based SARS-CoV-2 which is used as a vaccine candidate must be able to trigger an immune response, such as proteins from the spike (S), envelope (E), membrane (M) in SARS-CoV-2. This study aims to determine the potential for in silico epitope-based SARS-CoV-2 from S, EM, and SEM which is immunogenic, non-toxic, and non-allergenic. And evaluate the immune response by measuring the number of NK cells in the spleen and serum IgG levels in mice. Method: This research was carried out in 2 stages, an in silico exploratory study and an experimental study. The exploratory stage consisted of selecting immunogenic, non-toxic, non-allergic vaccine candidates, molecular docking tests, and epitope conjugation with an adjuvant in the form of ISCOM which was observed with a TEM microscope. The first group was the control, and the second group was given ISCOM. The remaining groups were each given the S, EM, and SEM epitope which had been conjugated with ISCOM and all were given orally. In 5 groups, NK cell levels were measured using a flow cytometer, while IgG levels were measured using Elisa. Research: The results of the in-silico test showed that 3 epitopes of S (FLVLLPLVSSQCVN), E (VNSVLLFLAFVVFLLVTLASS), and M (LYIIKLIFLWLLWPVTLACFV-LAAVY) were immunogenic, non-toxic, and non-allergic. Oral administration of in silico epitope-based SARS-CoV-2 in mice could increase the highest number of NK cells in the administration of S epitope. Meanwhile, the highest serum IgG level was given with the combination of SEM epitope. Conclusion: Oral administration of an in-silico epitope based on SARS-CoV-2 from spike, envelope, and membrane can increase the number of NK cells in the spleen and IgG levels in mice.
The use of urine lipoarabinomannan for establishing pulmonary TB in HIV patient: case report and systematic review Margono, Djiwa; Yulianti Ningtias, Pranudiah; Kusumaningrum, Ardiana; Prasetyo, Dimas Seto
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 2 (2023): Available online: December 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i2.38

Abstract

Introduction: Indonesia is still the country that provides the second-largest estimated number of TB cases worldwide. Several patients had TB with HIV-positive. HIV-positive patients with symptoms suggestive of pulmonary tuberculosis often difficult to produce sputum of sufficient quality. Lateral flow lipoarabinomannan examination with urine specimens is expected to be easy, inexpensive, fast, and accurate point-of-care testing. The aim of this research was to assess the sensitivity and specificity of the lateral flow method in detecting lipoarabinomannan (LAM) from urine specimens in HIV-positive patients with suspected pulmonary tuberculosis. Sputum cultures were utilised as a reference standard. Case Description: A 21-year-old male patient came to the clinic complaining of a cough that had not subsided since the previous month. Cough felt throughout the day with a little white phlegm. The patient admitted to having high-risk sexual behavior and had been tested positive for HIV but had not started antiretroviral therapy. The laboratory examination results showed that the leukocyte count was 3600/mm3 and the radiological examination revealed a chest X-ray within normal limits. The patient had been tested for sputum smears with negative results but was still advised to start TB treatment. The patient has not started treatment because he feels that his sputum smear test results are negative. Currently, the patient is not willing to have his sputum checked again. The polyclinic doctor knows the lipoarabinomannan (LAM) examination that uses urine specimens to diagnose TB but does not yet know its effectiveness for this patient. Conclusion: In this case report, the patient was unwilling to do a diagnostic test related to the TB. The systematic review was that the Alere Determine™ TB LAM Ag may not be clinically applicable for diagnosing tuberculosis infection in patients living with HIV at this time. However, Fujifilm SILVAMP TB LAM® could be regarded as a supplementary tool to the established diagnostic protocol for tuberculosis in HIV-positive patients.
Various types of extended spectrum β-lactamases: a literature review Rastuti, Made Rina; Budayanti, Ni Nyoman Sri; Dwija, Ida Bagus Nyoman Putra
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 2 (2023): Available online: December 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i2.52

Abstract

The inappropriate use of antibacterials causes the spread of resistance in bacteria and increases the health burden of infection due to the nature of resistance to many classes of antibiotics which are referred to as multi-resistant (multidrug resistant). Extended spectrum β-lactamase (ESBL) is one of the enzymes that cause multi-resistance, where bacteria with this enzyme become resistant to third-generation cephalosporin antibiotics which are often used to treat gram-positive and gram-negative bacterial infections in humans with minimal side effects. β -lactamase is an enzyme capable of hydrolyzing the β-lactam ring in β -lactam class antibiotics so that antibiotics become inactive. Mutations in the gene that encodes this enzyme produce a β-lactamase enzyme which can break down the β-lactam ring in all penicillin and cephalosporin antibiotics. The classification of β-lactamases is complex due to genetic, biochemical properties, and substrate affinity for β-lactamase inhibitors. There are also various types of ESBL enzyme-coding genes including TEM, SHV, CTX-M, VEB, PER, OXA, SFO-1, BEL-1, BES-1, TLA-1, and GES. This study aims to review various types of extended-spectrum β-lactamases.
Congenital neurosyphilis in neonatal sepsis by Leclercia adecarboxylata: a case report Tanaya, Putu Wahyu Dyatmika; Manuaba, Ida Ayu Sri Kusuma Dewi; Saputra, I Wayan Agus Gede Manik
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 2 (2023): Available online: December 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i2.54

Abstract

Introduction: Sepsis remains a leading cause of morbidity and mortality in neonates. In accordance with the development of diagnostic testing, various pathogens have been found to cause neonatal sepsis, ranging from common to even rarer pathogens. Leclercia adecarboxylata is a Gram-negative bacillus rarely found in neonatal sepsis and was previously found predominantly in environmental settings and the human gastrointestinal tract as commensal bacteria. We describe a rare case of Leclercia adecarboxylata in neonatal sepsis with congenital neurosyphilis. Case Description: We present a male neonate born from a positive syphilis serological test mother, presented with sepsis manifestation. Blood culture reveals growth of Leclercia adecarboxylata. The patient received both therapeutic regimens of antibiotics for sepsis and congenital syphilis. The cerebrospinal fluid analysis also performed revealed suggestive of congenital neurosyphilis. The patient passed away due to multiple organ failure. Conclusion: Neonatal sepsis and congenital neurosyphilis present with identical manifestations; therefore, appropriate recognition and comprehensive management should be done

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