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All Journal Jurnal Kedokteran Syiah Kuala Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease Majalah Obstetri dan Ginekologi Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya J-Dinamika: Jurnal Pengabdian Kepada Masyarakat YARSI Medical Journal Jurnal Respirasi (JR) JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Abdi Insani Majalah Biomorfologi (Biomorphology Journal) Jurnal Layanan Masyarakat (Journal of Public Service) Indian Journal of Forensic Medicine & Toxicology Archives of Pediatric Gastroenterology, Hepatology, and Nutrition Journal of Clinical Microbiology and Infectious Diseases Jurnal Widya Medika Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)

Eko Budi Koendhori, Eko Budi

Departemen Mikrobiologi Fakultas Kedokteran Universitas Airlangga

Author-ID : 508797

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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THE ANTI-TB DRUG SENSITIVITY OF Mycobacterium tuberculosis FROM CEREBROSPINAL FLUID AND BONE TISSUE BIOPSY SPECIMENS OF PATIENTS SUSPECTED TUBERCULOUS MENINGITIS AND SPINAL TB IN Dr SOETOMO HOSPITAL INDONESIA Mertaniasih, Ni Made; Kusumaningrum, Deby; Koendhori, Eko Budi; Harijono, Sugeng; Arky, Catur Endra; Putri, Jayanti; Urifah, Hanik
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 3 (2014)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar

Tuberculous meningitis (TBM) is an infection of meningens which potentially life threatening with significant morbidity andÂ mortality. Spinal TB has the same problem with TBM, infection in bone and joint, the delayed diagnosis worsens the prognosis. TheÂ rapid and accurate diagnosis plus promt adequate treatment is essential for the good outcome. The aim of this research is to study thefirst line drug sensitivity of Mycobacterium tuberculosis isolated from specimens of cerebrospinal fluid from suspected tuberculousÂ meningitis patients and bone tissue biopsy from suspected spinal TB patients. The method of this research is TB Laboratory examinationÂ in Department of Clinical Microbiology â Dr. Soetomo General Hospital, Indonesia, using the gold standard liquid culture methodÂ MGIT 960 System (Becton Dickinson) and solid culture method with Lowenstein-Jensen medium. The specimens CSF from 50Â TBM patients at January 2013 until May 2014. Positive isolate detection of Mycobacterium tuberculosis complex were 11 isolatesÂ (22%), which sensitivity 100% (11/11 isolates) to Rifampin (R), Pyrazinamide (Z), Ethambutol (E), and Streptomycin (S); one isolateÂ resistant to Isoniazid, sensitivity to Isoniazid 90,90% (10/11); and received 21 specimens of bone tissue biopsy which positive 5 isolates(23%), all isolates sensitive 100% (5/5 isolates) to Rifampin and Pyrazinamide, and 1 isolates resistant to Isoniazid, Ethambutol, andÂ Streptomycin, in which sensitivity 80% (4/5 isolates) to Isoniazid, Ethambutol, and Streptomycin. The conclusion of this research isÂ positivity detection 22% of CSF specimens, and 23% of bone tissue biopsy were low. All isolates sensitive 100% to Rifampin andÂ Pyrazinamide, and 80-90% sensitive to Isoniazid.

METODE MIKROKOLONI (SLIDE CULTURE) SEBAGAI METODE DIAGNOSTIC ALTERNATIVE YANG LEBIH CEPAT UNTUK DIAGNOSIS TUBERCULOSIS PARU Budi Koendhori, Eko; Harsono, Setio
Jurnal Kedokteran YARSI Vol 15, No 1 (2007): JANUARI - APRIL 2007
Publisher : Lembaga Penelitian Universitas YARSI

Despite wide distribution of pulmonary tuberculosis in Indonesia, its diagnosis is still an important issue to be dealt with. Fourty seven sputums from pulmonary tuberculosis patients in Surabaya were examined to detect Mycobacterium tuberculosis using three methods, i.e. the acidfast stain Ziehl Neelsen, microcolony (slide culture) and Lowenstein Jensen. Sputums were collected spontaneously from the patients. All of them were decontaminated and centrifuged. After the supernatant fluids were carefully decanted, the sediments were resuspended in 1 ml of 10 mMphosphate buffer (pH 7.4) and the suspensions were then inoculated on to two 76 x 13 mm glass microscope slides. One of them was stained by Ziehl Neelsen method and the other was inoculated into microcolony media for seven days and the waste suspension was inoculated into LowensteinJensen media. The results of the microcolony method analysis were compared with the Ziehl Neelsen staining. Employing McNemar test, a significant difference was observed between the microcolony method and the Ziehl Neelsen staining (?² = 5,88). The sensitivity and spesificity ofmicrocolony were 100% and 89% while the Ziehl Neelsen were 60% and 84% respectively. In conclusions microcolony method was better compared with the Ziehl Neelsen staining in the detection of Mycobacterium tuberculosis. Microcolony method was able to reduce time required todetect Mycobacterium tuberculosis in patient suspected with pulmonary tuberculosis.

Antibacterial Activity of Methanol Extract of Red Dragon Fruit Peel ( Hylocereus polyrhizus) against MethicillinSusceptible Staphylococcus aureus ( MSSA) ATCC 25923 and Methicillin Resistant Staphylococcus aureus (MRSA) In Vitro Shazia Hafazhah Aulia; Yuani Setiawati; Eko Budi Koendhori
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15963

Background: Staphylococcus aureus is one of the most virulent Staphylococcus which could cause systemic broad-spectrum infections and developed resistant strains. Red dragon fruit (Hylocereus polyrhizus) peel has a broad antibacterial activity against both gram-negative and positive bacteria, including Staphylococcus aureus. Objective: To determine and compare the antibacterial activity of methanol extract of red dragon fruit peel against Methicillin-Susceptible Staphylococcus aureus ATCC 25923 and Methicillin-Resistant Staphylococcus aureus. Method: Well diffusion test method was performed on 5 concentrations: 100%, 75%, 50%, 25%, and 0%, dissolved in DMSO 10% where at a concentration of 100% contains 1g/mL extract. The inhibition zone elicited as clear zone around the well then measured and analyzed with SPSS using One-Way ANOVA and Post Hoc LSD, to determine the significance of each concentration. Results: The mean diameter of the inhibition zone observed towards Methicillin-Susceptible Staphylococcus aureuswas 9.5333 ± 0.26822 - 15.4167 ± 0.22048, while bigger zone was found in Mueller-Hinton plate with Methicillin-Resistant Staphylococcus aureus which is 12.30 ± 0.20817 - 17.80 ± 0.25166 mm. Conclusion: This study showed that methanol extract of red dragon fruit peel has antibacterial activity against S. aureus, and shows higher antibacterial activity against Methicillin-Resistant Staphylococcus aureus than MethicillinSusceptible Staphylococcus aureus.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL LIDAH BUAYA (Aloe vera) TERHADAP BAKTERI Staphylococcus aureus Mia Rahardjo; Eko Budi Koendhori; Yuani Setiawati
Jurnal Kedokteran Syiah Kuala Vol 17, No 2 (2017): Volume 17 Nomor 2 Agustus 2017
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v17i2.8975

Abstrak. Staphylococcus aureus merupakan salah satu flora normal pada kulit, membran mukosa, orofaring, saluran pencernaan dan vagina yang berpotensi menjadi patogen. Pertumbuhan S. aureus yang berlebihan dapat menimbulkan infeksi yang serius baik di manusia atau hewan. Dan sekarang, beberapa S. aureus dikabarkan telah resisten terhadap antibiotik karena proses mutasi. Berdasarkan hal tersebut, penulis mencoba memberi alternatif pengobatan dengan memanfaatkan ekstrak etanol gel Aloe vera yang menurut beberapa penulis lain, gel Aloe vera mengandung antraquinone, tannin, polysaccharide, flavonoid, and saponin yang bersifat sebagai antibakteri. Jenis penelitian ini adalah eksperimental laboratorium dengan metode difusi dan dilusi. Penelitian ini menggunakan konsentrasi 100%, 75%, 50%, 25%, dan 0% pada metode difusi. Sementara itu metode dilusi menggunakan konsentrasi 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,5625% kontrol positif (+), dan kontrol negatif (-).Dari pengamatan hasil penelitian, tidak didapatkan zona inhibisi pada metode difusi serta tidak dapat ditentukan konsentrasi hambat minimum (KHM) dan konsentrasi bunuh minimum (KBM) terhadap pertumbuhan Staphylococcus aureus. Hal ini terkait dengan rendahnya senyawa aktif yang digunakan di sampel gel Aloe vera dalam penelitian ini akibat pengaruh dari faktor lingkungan, perbedaan usia tanaman dengan literatur awal, proses degradasi dan reaksi enzimatik, adanya perbedaan metode ekstraksi, serta proses oksidasi saat terpapar oleh udara. Berdasarkan hal tersebut, dapat disimpulkan bahwa aktivitas antibakteri dari ekstrak etanol gel Aloe vera terhadap Staphylococcus aureus tidak dapat ditentukandengan metode difusi dan metode dilusi. (JKS 2017; 2: 65-71) Kata Kunci : Gel lidah buaya (Aloe vera), Staphylococcus aureus, antibakteri, metode difusi dan dilusi. Abstract. Staphylococcus aureus is one of the normal flora in human skin, mucous membrane, oropharynx, gastrointestinal tract, and vagina which potentially becomes a pathogen. The excessive growth of S. aureus can cause many serious infection whether in human or animal. And nowadays, some of S.aureus have become resistant to antibiotic caused by its mutation. According to that case, researcher try to find an alternative solution by using Aloe vera gel ethanol extract that some other researchers say it contains antraquinone, tannin, polysaccharide, flavonoid, and saponin as anti bacterial compound. This research aimed to find out the effectiveness of Aloe vera gel ethanol extract in inhibiting Staphylococcus aureus.This research is designed as an laboratorium experimental with difusion and dilusion method. Test performed with using 100%, 75%, 50%, 25%, and 0% concentration in difusion method and using 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,5625% concentration , positive control (+) and negative control (-) in dilution method. There is no inhibition zone in difusion method, also no minimum inhibitory concentration and no bactericidal concentration can be seen in dilution method that inhibit the growth of Staphylococcus aureus. This result might be related to the minimal amount of active compound in this sample, that is taken from Aloe vera gel. The amount of active compound can be influenced by the environment, difference in Aloe’s age, degradation process and enzymatic reaction, difference in extraction method and also influenced by oxidation process when it’s exposed to air. Based on the results, anti bacterial activity of Aloe vera gel ethanol extract towards Staphylococcus aureus can not be determined in difusion and dilution method. (JKS 2017; 2: 65-71) Key words : Aloe vera gel, Staphylococcus aureus, antibacterial, difusion and dilusion method

Comparison of Aloe vera leaves ethanol extract effect against Escherichia coli ESBL and Klebsiella pneumoniae ESBL Dewinta Enggar Pramesthi; Yuani Setiawati; Eko Budi Koendhori
Jurnal Kedokteran Syiah Kuala Vol 20, No 2 (2020): Volume 20 Nomor 2 Agustus 2020
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v20i2.18502

Abstrak. Aloe vera merupakan tanaman herbal yang mengandung beberapa zat aktif yang telah terbukti mempunyai efek antibakteri untuk berbagai macam bakteri. Dapat ditemukan banyak infeksi yang disebabkan oleh bakteri gram negatif. Beberapa bakteri gram negatif ini memproduksi enzim β lactamase mutan, yang diketahui sebagai Extended Spectrum Beta Lactamase (ESBL). Bakteri ESBL resisten terhadap beberapa macam antibiotik dalam golongan beta laktam, hal ini menjadi salah satu permasalahan utama di rumah sakit. Berdasarkan latar belakang yang ada, tujuan dari studi ini adalah untuk membandingkan efek antibakteri dari ekstrak etanol daun lidah buaya terhadap Escherichia coli ESBL dan Klebsiella pneumoniae ESBL. Studi ini merupakan eksperimental laboratorium. Konsentrasi Hambat Minimal (KHM) dan Konsentrasi Bunuh Minimal (KBM) ditentukan dengan metode dilusi. Konsentrasi yang digunakan pada penentuan KHM untuk kedua bakteri adalah 90%; 80%; 70%; 60%; 50%; 40%; 30%; dan 20%. Penentuan KBM dilakukan dengan cara penanaman suspensi dari muller hinton broth pada nutrient agar plate. Hasil yang didapatkan dianalisis menggunakan metode deskripsi. KHM untuk kedua bakteri ditemukan pada konsentrasi yang sama yaitu 80% (8 g ml-1). KBM untuk kedua bakteri juga ditemukan pada konsentrasi yang sama yaitu 80% (8 g ml-1). Penentuan KHM dan KBM dilakukan sebanyak 10 kali replikasi. Dapat disimpulkan bahwa ektrak etanol daun lidah buaya tidak mempunyai efektivitas yang berbeda terhadap Escherichia coli ESBL dan Klebsiella pneumoniae ESBL. Kata kunci: Escherichia coli ESBL - Klebsiella pneumoniae ESBL - Ekstrak etanol daun lidah buaya- antibakteri - dilusi Abstract. Aloe vera is a herbal which has some active substances that have been proven for its antibacterial effect for some bacterias. There are many infections caused by gram negative bacterias. Some of gram negative bacterias produce mutant β lactamase enzyme, that known as Extended Spectrum Beta Lactamase (ESBL). ESBL bacteria are resistant to some antibiotics in beta lactam class and become one of main problem in hospital. Based on this background, the aim of this study was to compare the antibacterial activity of aloe vera leaves ethanol extract between Escherichia coli ESBL and Klebsiella pneumoniae ESBL. This study was a lab experimental. Minimum Inhibitory Concentration (MIC) values and Minimum Bactericidal Concentration (MBC) values were determined by dilution method. The concentration used in MIC determination for both bacteria is 90%; 80%; 70%; 60%; 50%; 40%; 30%; and 20%. The MBC values were determined by suspension streaking from muller hinton broth on nutrient agar plate. The result is analyzed with description method. The MIC value for Escherichia coli ESBL and Klebsiella pneumoniae ESBL is found in the same concentration which is 80% (8 g ml-1). The MBC values for Escherichia coli ESBL and Klebsiella pneumoniae ESBL also found in the same concentration 80% (8 g ml-1). MIC and MBC values have been proved in the first until tenth replications. Thus, aloe vera leaves ethanol extract has no different effectiveness against Escherichia coli ESBL and Klebsiella pneumoniae ESBL. Keywords: Escherichia coli ESBL - Klebsiella pneumoniae ESBL - Aloe vera leaves - antibacterial - dilution method

Peran Ethanol Extract Propolis sebagai terapi penunjang pada mencit yang diinfeksi dengan Mycobacterium tuberculosis Eko Budi Koendhori; Ni Made Mertaniasib; Yuliati Hood; M. Amin M. Amin
Jurnal Kedokteran Syiah Kuala Vol 9, No 2 (2009): Volume 9 Nomor 2 Agustus 2009
Publisher : Universitas Syiah Kuala

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Abstrak. Tuberkulosis masih menjadi penyakit infeksi yang serius di dunia. Menurut laporan WHO, penyakit ini menginfeksi sepertiga penduduk dunia. Menyebabkan 2 juta kematian tiap tahun dan hampir 8 juts penderita baru. Beberapa tahun terakhir jugs dilaporkan adanya peningkatan kuman tuberkulosis yang tahan terhadap obat anti tuberkulosis (MDR-TB). Pada sisi lain, sejak 50 tahun yang lalu beluro ditemukan adanya obat anti tuberkulosis baru, Riset ini dilakukan dalam rangka untuk memberikan pandangan baru terhadap peran imunomodulator alami (EEP) sebagai obat penunjang dalam pengobatan tuberkulosis. 100 ekor mencit dikelompokkan menjadi grup 5 dan grup 12. Tiap grup dipisahkan lagi menjadi 5 kelompok yang lebih kecil yaitu grup control, EEPlOO, 200, 400 dan 800. Mencit kemudian diinfeksi dengan Mycobacterium tuberculosis intranasal. 4 minggu kemudian semua mencit diberi isoniazid 25 mg/kgBB per oral .. grup EEP diberi EEP per oral l 00, 200, 400 dan 800 mglkgBB sesuai grupnya, sedang grup kontrol tidak. Pada minggu ke 5 semua mencit grup S diterminasi dengan jalan pemberian eter overdosis dan grup 12 diterminasi pada minggu ke 12 dengan cara yang sama, Paru-paru mencit diambil secara aseptic untuk dilakukan pemeriksaan histopatologi. Untuk konfirmasi perbedaan antara perlakuan dan kontrol digunakan uji annova dan uji T dengan p0,05. Basil pada grup 5 dan 12 memperlihatkan bahwa EEP dapat menurunkan kerusakan jaringan paru, skor Dorman untuk kontrol, EEPIOO, 200, 400 dan 800 mg/kgBB berturut-turut adalah 13,55; 10,05; 9,3; 8,65 dan 11,45 untuk grup 5 sedang untuk grup 12 berturut-turut adalah 18,l; 14; 11,7;10,1 dan 9,4. Sebagai kesimpulan kami laporkan bahwa EEP dapat mengurangi kerusakan jaringan paru pada mencit yang diinfeksi denganMycobacterium tuberculosis. (JKS 2009;2:6.S-70) Kata kunci : Mycobacterium tuberculosis, EEP, IFN--y, lung damage. Abstract. Tuberculosis still the leading infection disease in the world. According to WHO report, it affected almost one thirth people in the world. It caused 2 millions death anually and almost 8 millions new people infected with this bacteria every year. In the last years, it has been reported that multidrug resistentMycobacterium tuberculosis (MDR-TB) have increased. On the other band. since SO years ago no new drug for tuberculosis have been reported. This research conducted to give a new view of natural immunomoduJator(ethanol extract propolis/EEP) as an adjuvant therapy for tuberculosis. Hundred mice separated into two groups, group S and 12. Each group separated again into 5 small groups, control, EEP 100, EEP 200, EEP 400 and EEP800. Mice infected with Mycobacterium tuberculosis intranasal. 4 weeks later, they were given isoniazid 25mg/kgBW orally. The EEP's groups were given ·EEP 100, 200, 400 and 800 mg/kgBW respectively but the control groups were not. At the end of S 'th week, all mice in groups S were terminated by overetherisatioo and group 12 were terminated at the end of 12'th weeks. Mice lung were separated aseptically, examination of histopathologic were done as described on methodology. We used the Anova and T test to confirm the differentiation between control and EEP's groups with p0,05. In group 5 and 12 the result showed that EEP could reduce lung damaged, Dormans scores for control, EEP 100, 200, 400 and 800 mg/kgBW respectively were 13,55; 10,05; 9,3; 8,65 and 11,45 for group 5, and 18,1; 14; 11,7;10,l and 9,4 for group 12. In summary we reported that EEP could reduce the lung damages do to Mycobacterium tuberculosis. (JKS 2009;2:65-70) Key word: Mycobacterium tuberculosis,EEP, IFN-y,lung damage

Infeksi Saluran Kemih Sebagai Faktor Risiko Terjadinya Ancaman Persalinan Preterm Henky Mohammad Masteryanto; Gatut Hardianto; Hermanto Tri Joewono; Eko Budi Koendhori
Majalah Obstetri dan Ginekologi Vol. 23 No. 2 (2015): Mei - Agustus 2015
Publisher : Universitas Airlangga

Tujuan: Mengidentifikasi kuman penyebab infeksi saluran kemih sebagai faktor risiko terjadinya ancaman persalinan preterm.Bahan dan Metode: Penelitian ini adalah penelitian analitik observasional cross sectional, dilakukan di Kamar Bersalin dan Poli Hamil RSUD Dr. Soetomo serta di Bagian Mikrobiologi Kedokteran Fakultas Kedokteran Universitas Airlangga Surabaya, pada bulan Januari-April 2015. Penghitungan jumlah koloni dan identifikasi kuman dilakukan melalui pemeriksaan kultur urine porsi tengah dan dilanjutkan dengan tes kepekaan antibiotika. Analisa statistik menggunakan uji Chi square untuk jumlah koloni kuman dan bakteriuria, dan uji Fischer exact untuk jenis kuman, menggunakan perangkat lunak SPSS 20. 0.Hasil: Sampel terdiri dari 20 wanita hamil dengan ancaman persalinan preterm/partus prematurus iminens (PPI) dan 20 wanita hamil normal tunggal dengan usia kehamilan 28-36 minggu. Perbandingan antara jumlah koloni kuman kedua kelompok secara statistik tidak signifikan, dengan nilai p=0,063 (p>0,05), perbedaan jenis kuman yang ditemukan antara kedua kelompok secara statistik tidak signifikan, dengan nilai p=0,058 (p>0,05).Simpulan: Pada kehamilan dengan ancaman persalinan preterm 80% didapatkan pertumbuhan kuman Staphylococcus epidermidis (30%) dan Escherichia coli (15%). Jumlah koloni kuman aerob urine pada kehamilan dengan ancaman persalinan preterm lebih banyak dibandingkan dengan kehamilan tanpa ancaman persalinan preterm, tetapi secara statistik tidak berbeda bermakna. Risiko terjadinya PPI pada wanita hamil dengan jumlah koloni kuman urine >105 cfu/mL 3 kali lebih besar dibanding wanita hamil dengan jumlah koloni <105 cfu/mL. Jenis kuman aeroburine pada kehamilan dengan dan tanpa ancaman persalinan preterm hampir sama.

Retrospective Study of Self Esteem in Patients with Pityriasis Versicolor Bagus Meurah Suropati; Eko Budi Koendhori; Sawitri Sawitri; Evy Ervianti
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 32 No. 2 (2020): AUGUST
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikk.V32.2.2020.93-97

Background: Pityriasis Versicolor (PV) is a fungal infection of the skin, caused by the Malassezia sp., a genus of fungi. Patients commonly present with bordered skin patches of white, grey, and sometimes brown on their skin surface. The prominent changing of skin colour in exposed areas of the skin may cause feelings of insecurity to the patients and become limelight for other people, impacting their self esteem. Purpose: To evaluate the self esteem profile in PV patients at the dermatovenereology outpatient clinic of Dr. Soetomo General Academic Teaching Hospital Surabaya. Methods: This was a retrospective descriptive study based on medical records of the dermatovenereology outpatient clinic of Dr. Soetomo General Academic Teaching Hospital Surabaya. The data were collected from June 2018 to May 2019. This research evaluates PV patients's profiles by age, sex, education level, occupation, residential environment, site of infection, duration of the infection, and self esteem. The patient's self esteem was assessed using the Rosenberg Self Esteem Scale (RSES). Result: Fiftythree PV patient’s self esteem values were successfully recorded. Of the 53 patients, the score was low in 3 people (5.67%), the normal score in 50 people (94.33%), and no one had a high score. Of the 3 people with low self esteem scores, 2 of them were men, consisting of 1 teenager and 2 adults, having basic education levels, who were students and housewives. The three patients had the infection for more than 3 months and they resided in Tambaksari, Wonocolo, and outside Surabaya. Conclusion: The majority of PV patients at the dermato-venereology outpatient clinic of Dr. Soetomo General Academic Teaching Hospital have normal self esteem scores.

The Prevalence of Extended Spectrum Beta-Lactamase (ESBL) Producing Gut Bacterial Flora Among Patients in Dr. Soetomo Hospital and Primary Health Centre in Surabaya Dian Neni Naelasari; Eko Budi Koendhori; Linda Dewanti; Rosantia Sarassari; K Kuntaman
Folia Medica Indonesiana Vol. 54 No. 4 (2018): December
Publisher : Faculty of Medicine, Universitas Airlangga

The extended-spectrum b-lactamase (ESBL) producer bacteria until now were mostly identified in hospital environment. The aim of this study was to analyze the prevalence of ESBL-producing gut flora and distribution of ESBL encoding genes between hospitalized patient in Tropical Wards of Dr. Soetomo Hospital and patient from a primary health centre (PHC) as community environment in Surabaya. Thiry rectal swab samples from hospital of Dr. Soetomo patients and from PHC (60 samples in total) were collected for this study. Samples were screened in MacConkey agar supplemented with 2 mg/L of cefotaxim, incubated at 37ºC for 24 hours. Then the growing colony were confirmed with Disk Diffusion Synergy test (DDST) for diagnosis of ESBL producer. The identified ESBL producers were then identified the bacteria species by biochemical method. ESBL gene were detected by PCR with specific primers. The results showed that there was not difference of positif nuber of ESBL-producing bacteria gut floral between patients of Dr.Soetomo Hospital, 25/30 (83.3%) and PHC, 11/30 (36.7%) (p=1). The pattern of ESBL gene distributions among samples from hospital showed that SHV was 12%, TEM was 36%, and CTX-M was 80%, and from PHC were SHV 18.2%, TEM 27,3% and CTX-M 81,8%. Statistical analysis showed that the pattern was not significantly different among hospitals and PHC samples as shown by SHV gene (p=0,631), TEM (p= 0.715), and CTX -M (p=1). From each ESBL gene, the dominant genes that found producing ESBL were the CTX-M genes followed by TEM and SHV genes. The prevalence of ESBL producersin intestinal flora of both the hospital (83,3%) and the PHC (36,7%) was very high. There was not significant difference between the prevalence of ESBL producer in gut flora of hospitalized patients compared to PHC. There was found other patterns of ESBL gene combinations in the hospital of SHV+CTX-M genes, TEM+CTX-M, SHV+TEM+CTX-M genes and PHC, the combination pattern of SHV+CTX-M, TEM+CTX-M.

Phylogenetic and Transmission Patterns of Extended-Spectrum β- Lactamase –Producing Escherichia Coli among Neonatal’s Gut Flora in Indonesia Rakhmatul Binti Sulistya; Kuntaman Kuntaman; Eko Budi Koendhori; Terza Aflika Happy; Wahyu Setyarini
Folia Medica Indonesiana Vol. 57 No. 3 (2021): September
Publisher : Universitas Airlangga

This study aimed to explore the phylogenetic pattern of Extended-spectrum β-lactamase-producing Escherichia coli in the gut flora of neonatal into groups A, B1, B2, D and tracing the presence of spreading among the age group of neonatal. Multiplex PCR was conducted to classify the phylogenetic group of ESBL producing E. coli into groups of commensals (A, B1) and pathogen (B2, D). The spread of bacterial agents among the host (neonatal) was conducted by the RAPD-PCR method with two random primers. Among 34 bacterial isolates were identified among early neonatal group A (22,2%), B1 (11,1%), B2 (22,2%) D (33,3%) and postneonatal groups A (8%), B1 (4%), B2 (36%) D (40%). It was not a statistically significant difference (p=0.388). The dominance of pathogenic groups B2 and D as many as 70,6%. From 34 samples was found 34 types of RAPD indicated there were not any bacterial spread among neonatal. The ESBL producing E. coli among early neonatal and postneonatal were dominated by the pathogenic group of B2 and D (total=70,6%), but they were not statistically significant. There was not any spreading of bacterial agents among individual gut flora of neonatal.

Co-Authors ACHMAD RIFAI Alimsardjono, Lindawati Alpha Fardah Athiyyah Anak Agung Putri Nadia Paramitha Andy Darma Andy Setiawan Arthur Pohan Kawilarang Ayu Lidya Paramitha Bagus Meurah Suropati Bambang Purwanto Betty Agustina Tambunan Catur Endra Arky, Catur Endra Cynthia Dwi Ramadhanie Deby Kusumaningrum Dewi Ratna Sari Dewinta Enggar Pramesthi Dian Neni Naelasari Diani Dwi Indrasari Dimas Firman Hidayat Eddy Bagus Wasito Endraswari, Pepy Evy Ervianti Fernanda Toriq Ainur Rochman Gatut Hardianto, Gatut Hanik Urifah, Hanik Harsono, Setio Henky Mohammad Masteryanto Hermanto Tri Joewono I Gusti Made Reza Gunadi Ranuh Imam Susilo Irfan Arif Ikhwani Ismi Masyithah Jayanti Putri, Jayanti Juita, Liza Puspa Senja Asmara Juniastuti Juniastuti Kadariswantiningsih, Ika Khadijah Rizky Sumitro Kuntaman Kuntaman Kusumaningrumm, Deby Lestari, Aprilia Dwi Linda Dewanti Lindarto, Wira Widjaya M. Amin M. Amin ManikRetno Wahyunitisari Mia Rahardjo Muhammad Aafi Baharuddin Attamimi Muna, Nafdzu Mahmudatul Muna, Nafdzu Makhmudatul Nasicha, Arifatun Ni Made Mertaniasib Ni Made Mertaniasih Ni Njoman Juliasih Nurul Wiqoyah, Nurul Nuswantoro, Djohar Pepy Dwi Endraswari, Pepy Dwi Priyo Budi Purwono Putri, Naomi Lesmana Raihan Akbar Muhammad Rakhmatul Binti Sulistya Ratna Kusumawati Ratna Kusumawati Rebekah Setiabudi, Rebekah Rosantia Sarassari S. Soedarsono Sardjono, Lindawati Alim Sawitri Sawitri Semita, I Nyoman Setiawan, Firman Setya Wijoyo, Dhia Lintang Shazia Hafazhah Aulia Silvia Sutandhio Sri Purwaningsih Steven Christian Susianto Subijanto Marto Sudarmo Sugeng Harijono, Sugeng Terza Aflika Happy Titiek Sulistyowati Titiek Sulistyowati Ummi Maimunah Usman Hadi Vermasari, Naritha Wahyu Setyarini Wahyunitisari, Manik Retno Wardhani, Puspa Widyatama, Fikri Sasongko Wiwin Retnowati Yelvi Levani Yitijuatni Yuani Setiawati Yudayanti, Elprania Credo Yuliati Hood

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