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I Wayan Suardana
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Journal of Veterinary and Animal Sciences
Published by Universitas Udayana
ISSN : 25501283     EISSN : 26220571     DOI : -
Core Subject : Health,
Veterinary
It covers all the scientific and technological aspects of veterinary medicine in general, anatomy, physiology, biochemistry, pharmacology, microbiology, pathology, public health, parasitology, infectious diseases, clinical sciences, biotechnology, alternative veterinary medicine and other biomedical fields. In the field of animal science, the journal receives original manuscripts covering breeding and genetics, reproduction and physiology, nutrition, feed sciences, animal products, biotechnology, behavior, livestock farming system, socio-economic, and policy.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 11 Documents
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Search results for , issue "Vol 1 No 1 (2017)" : 11 Documents clear
Phenotypic Identification of Lactobacillus from Breast Milk Based on Their Ability to Ferment Sugars Ni Made Indri Hapsari Arihantana; Ni Nyoman Puspawati
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p05

Abstract

The purpose of this study was to identify the species of Lactobacillus which has the potency as superior probiotic based on their ability to ferment sugars. The study was conducted in two steps, the first was a confirmation test of the five lactic acid bacteria (LAB) isolates of breast milk A1, A6, A8, B8, and B10b. All of the LAB isolates were Gram positive rod and negative catalase. This shows that all of the isolates were of the genus of lactic acid bacteria and readily for the second step testing. The second step was to determine the LAB isolates ability to ferment 50 sugars (carbohydrates) using API 50 CHL kit. Results from the API 50 CHL kit test showed that the 5 LAB isolates were of indigenous LAB with the identification level of 88,7% - 99,9%. The species found were three spesies of Lactobacillus plantarum-1 (Lactobacillus plantarum-1 A1, Lactobacillus plantarum-1 A8, Lactobacillus plantarum-1 B8),and 2 spesies of Lactobacillus rhamnosus (Lactobacillus rhamnosus A6, and Lactobacillus rhamnosus B10b), respectively.
Identification of Growth Hormone Gene of Bali Cattle with Qualitative Superior in Bali Province Ni Ketut Suwiti; I Wayan Suardana; Ni Luh Watiniasih; I Nengah Kerta Besung; Chandra Yowani
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p09

Abstract

A research has conducted, in order to identify the genetic markers of Bali cattle that can be used as an indicator of their qualitative superior. The genetic marker identified was the growth hormone gen. As many as 50 whole blood samples which were collected from 50 individual cattle which were certificated as good breed or had qualitative superior, were used in this study. The DNA fragments were identified by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with primers, GH5 (5’-CCC ACG GGC AAG AAT GAG GC-3’ and GH6 (5’TGA GGA ACT GCA GGG GCC CA-3), respectively. Furthermore, the PCR products were restricted with MspI endonucleic restriction enzyme, before electrophoresis in 2% agarose The result showed that the growth hormone gene of bali cattle with qualitative superior has polymorphism which characterized by the formation of two fragments i.e. 230 and 329 bp that categorized as: allele +/+ and allele -/-.
Antimicrobial Susceptibility Patterns And DNA Plasmid Profiles Of Escherichia Coli O157:H7 Isolated From Feces Of Chicken I Wayan Suardana; Iwan Harjono Utama; Michael Haryadi Wibowo
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p01

Abstract

Escherichia coli O157:H7 has been identified as one of the most devastating microorganism causing diseases in human. The infection by this bacteria causes a wide spectrum of clinical manifestations ranging from asymptomatic, diarrhea or bloody diarrhea, up to serious clinical conditions such as hemorrhagic colitis, and hemolytic uremic syndrome. Poultry is known as one of its reservoir besides cattle and pig. Moreover, the use of antibiotic in animal feed as growth promoters is common worldwide. Due to its potential threat to public health, sensitivity test against various antibiotics and studying the plasmid DNA profiles are necessary. The study was initiated by cultivation of isolates, followed by test of antimicrobial susceptibility against various antibiotics and then by analyzing the plasmid DNA profiles. Results of study showed that among of 7 E. coli O157:H7 local isolates originated from chicken feces, as many as 42.9%; 14.3%; 14.3%; and 14.3% were resistant to 2; 3; 4; and 5 various of antibiotics, respectively. Base on the type of antibiotic, 85.7%; 71.4%; and 42.9% showed resistance to Methicillin, Penicillin G, Doxycycline and Streptomycin. Plasmid DNA of each isolates showed characters variation too, ranging from 2 to 4 bands with molecular weight ranging from 19.457 bp; 15.137 bp, 5.749 bp, 4.118 bp, 3.500 bp, 2.957 bp and 2.024 bp. Results of study indicated that local isolates of E. coli O157:H7 isolated from chicken showed multiple antibiotic resistance against various antibiotics, as well as its plasmid DNA profiles.
Macroconidia of Dermatophytes Fungi on Direct Microscopic Examinations Putu Ayu Sisyawati Putriningsih; I Putu Gede Yudhi Arjentinia
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p10

Abstract

Dermatophytosis (ringworm) caused by dermatophytes fungi is one of the cattle diseases that can infect Bali cattle. Ringworm significantly cause economic loss to farmers and at the same time is zoonotic disease. Diagnosing dermatophytosis based on the clinical signs in the animals, followed by direct microscopic examination of skin scrapings and hair samples and confirmed by laboratory tests. On direct microscopic examination of skin scrapings and hair samples, generally detected the elements of the fungi such as arthrospores or hyphae. In this study, skin scrapings and hair samples from Bali cattle that clinically suffered ringworm were collected. Samples were then placed on an object glass, added drops of 10% KOH, covered with a cover glass, and after 10-15 minutes were examined under a microscope with a magnification of 100X and 400X. We were able to detect macroconidia as well as arthrospores of the fungi. Macroconidia were detected on two of seven samples tested (28.57%). Normally, macroconidia will not be detected prior culturing on agar media and staining with Lactophenol Cotton Blue (LPCB). Dermatophytes fungi produces two types of asexual propagule: the saprophytic conidia and parasitic conidia, depending on the environment where they grow. Macroconidia that were detected in this study are saprophytic conidia possibly from the soil and/or from broken or hair loss. This macroconidia might be a potential source of infection.
The Performance of Balinese Gilt Reared Intensively and Supplemented With Probiotic K. Budaarsa; A. W. Puger; T. I. Putri; I D.G.A. Udayana
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p06

Abstract

Study to improve the performance of Balinese gilt reared by intensive management including good quality rationssupplemented with yeast culture as a source of probiotics has been conducted. Randomized Block Design (RBD) were used in this study. Sixteen post weaning (two months old) gilts weight 10-12kg were divided into 4 groups: (i)ration control, R1 (ration containing 18% concentrate, 40% corn and 41% rice bran and 1% mineral); (ii) R2 (R1+ 0.20% probiotics; (iii) R3 (R1+ 0.25% probiotics and R4 (R1 + 0.30% probiotics) with 4 replications. Parameters measured including: feed intake, body weight gain, feed conversion ratio (FCR) and digestibility of the ration. Results showed that feed intake, daily weight gain and feed digestibility increased significantly (P <0.05) in the gilt receiving probiotic treatment, on the other hand the value of FCR decreased significantly compared to the control group. It is concluded that the addition of probiotics in rations significantly improve the performance of Balinese gilt.
Patological Changes in Liver and Gall Bladder Of Bali Cattle Infected by Fasciolosis I Made Kardena; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Ketut Berata; Ida Bagus Windia Adnyana; I Made Sukada; Kadek Karang Agustina; Putu Agus Trisna Kusuma Antara
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p02

Abstract

Fascioliosis is a parasitic disease that infects ruminants and the disease is widely spread in the world. Fascioliosis caused by Fasciola hepatica and Fasciola gigantica that can cause macroscopic and microscopic lesions in the liver and gall bladder of bali cattle. Samples of bali cattle in Pesanggaran slaughter house that infected with fasciolosis were used in this study. The pathological macroscopic and microscopic changes of the liver and gall bladder were observed. The parasite found in the liver and gall bladder, thickening of bile duct mucous were observed on macroscopic examination. However, in microscopic observation found infiltration of inflammatory cells, fibrosis, necrosis, and degeneration of hepatocytes. In the gall bladder, necrosis was found in epithelial mucosal bile duct, infiltration of collagen fibers, inflammatory cells, hypertrophy and hyperplasia of the bladder epithelium were occurred.
Cover, Editor, Table of Contents Journal of Veterinary and Animal Sciences
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

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Abstract

Cover, Editor, Table of Contents
The Characteristic of Egg Drop Syndrome Virus of Medan Isolate Gusti Ayu Yuniati Kencana; I Nyoman Suartha; Arini Nurhandayani; Syamsidar Syamsidar
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p04

Abstract

Egg Drop Syndrome (EDS) is a poultry disease marked by a decrease of egg production up to 40% with a declined quality of eggs. In Indonesia, EDS is astrategically infectious disease that must be eradicated. Until now, EDS vaccines are available, vaccinations on egg-laying chicken have been conducted nevertheless the case of EBS are still found. This research collaborates with PT Sanbio Laboratories (Poultry vaccine producer in Bogor) aimed to prepare EDS seed vaccines from local isolates. Isolate samples are collected from ten egg-laying chicken farmswith signs of declined production and egg quality as well as unevenly shaped and thinning of egg shells. Virus isolation was conducted on embryonated duck eggs at the age of 11 days through the allantoic space. Embryonated duck eggs are then incubated for 3 days in the incubator at 370C and are observed daily. On the third day, the eggs are taken out of the incubator and then inserted in the cooler overnight. The allantoic fluid is harvested on the third day post inoculation; it is identified with haemaglutination test and Polymerase Chain Reaction (PCR). Six isolates are positively identified as EDS virus: two from the Medan isolates, there from the Rumpin isolates, and one from Surabaya isolate. One isolate is chosen; which was the EDS isolate of Medan, with the highest titer for passaging and characterization. The content of the virus is calculated with Reed and Muench formula and expressed in a unit of Egg Lethal Dose 50 (ELD50). Research results shows titer of Medan EDS virus isolate after the second passage was 1012 HA Unit, with virus content of 109,5 EID50, length base product of PCR that was successfully amplified as 500bp. Therefore the Medan isolate is a recommended candidate for EDS vaccine seeds.
Herbal Extract as An Antibacterial Against Gram Positive Bacteria Causing Dermatitis Complex I Nyoman Suartha; I GustiKetut Suarjana; Luh Made Sudimartini; I Made Merdana; I Made Dira Swantara
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p07

Abstract

Study in order to evaluate the antibacterial effect of herbal extract from leaves of three different species of plant namely neem(Azadirachtaindica A. Juss), gotu-kola (Centellaasiatica), andsoursop (Annona muricata L) against bacteria causing dermatitis complex in dogs have been undertaken. The antibacterial activities of the herbal extract were evaluated using the agar diffusion test with extract at three different concentrations 5%, 10%, and 25%, respectively.Staphylococcus aureus was isolated from the dermatitis complex cases in dogs. The results showed various antibacterial activities of all the herbal extract at all concentration as indicated by the diameter of inhibition haloes produced around the orifice. The average inhibition haloes for each herbal extract concentration was 2.19± 0.53 mm; 4.32± 0.84 mm; 5.86± 0.79 mm, respectively. It can be concluded that herbal extract from the three plants is a potential antimicrobial against agents causing dermatitis complex.
Detection of Haemophilus Parasuis from The Respiratory Tract of Pigs I Gusti Ketut Suarjana; I Nengah Kerta Besung; Ketut Tono PG
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p03

Abstract

Study in order to detect the presence of Haemophilus parasuis in pig farms in Tabanan, Badung, and Gianyar Bali have been undertaken. A total of 197samples including nasal and tracheal swabs, organs and synovial fluids were collected from 69, 28 and 100 animals from pig farms in Tabanan, Badung and Gianyar regency, respectively. Samples were collected mainly from animals which showing clinical signs of coughing, asphyxiate, swollen joints and death animals. Isolation and identification of Haemophilus spp was performed following the methods described by Priadi et.al. (2004) and Nedbalkova et al. (2006) with slightly modification. Antimicrobial sensitivity test was performed using the disc diffusion method by Kirby Bauer.Haemophilus parasuis was detected from one sample which shown resistant to streptomycin only and sensitive to ampicillin, doxycycline and kanamycin, respectively.

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