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Direktorat Politenik Kesehatan Kemenkes Bandung Jl.Pajajaran No 56 Bandung
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Jurnal Kesehatan Siliwangi
Published by Politeknik Kesehatan Kemenkes Bandung
ISSN : -     EISSN : 28071530     DOI : https://doi.org/10.34011/jks.v4i1.1671
Core Subject : Health, Science, Social,
Biochemistry, Genetics & Molecular Biology Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology
Jurnal Kesehatan Siliwangi menerima publikasi dalam makalah penelitian artikel asli, makalah tinjauan, laporan kasus, pengabdian masyarakat, penelitian kualitatif/dan kuantitatif di Indonesia atau negara lain untuk memberikan pemahaman mengenai aspek kesehatan. Ruang lingkup Artikel yang diterbitkan dalam jurnal ini adalah bidang : - Analis Kesehatan/ Teknik Laboratorium Medik - Gizi - Farmasi - Keperawatan Gigi - Keperawatan - Kebidanan - Kesehatan Lingkungan - Promosi Kesehatan
Arjuna Subject : Kedokteran - Kesehatan Masyarakat, Kesehatan Lingkungan dan Pekerjaan
Articles 532 Documents
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OPTIMASI VARIASI VOLTASE DAN WAKTU TERHADAP KUALITAS PITA DNA ESCHERICHIA COLI PADA PROSES ELEKTROFORESIS GEL AGAROSA nur amani putri, afifah; Iin Nur Indra, Asep; Merdekawati, Fusvita; Khoirul Abror, Yogi
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Background: Escherichia coli is one of the causes of foodborne illness. Conventional PCR is a PCR method that is carried out qualitatively followed by visualization on agar electrophoresis. Agarose gel electrophoresis is a technique that is often used in various fields of science to separate a mixture of DNA on agarose substrate. This method is used to perform qualitative analysis of DNA samples. In electrophoresis, there are factors that affect the movement of DNA molecules, one of which is voltage. In addition, the length of time of the electrophoresis process can also affect the effectiveness of the results and the rate of migration. When the electrophoresis process time is short, large DNA fragments still tend to stick. Purpose: Therefore, it is necessary to balance the voltage and time given to get good DNA banding results. Methods: The research unit that will be used is the result of amplification of Escherichia coli 16SrRNA gene DNA measuring 584 bp. The electrophoresis process was carried out with voltage variations of 50, 100, 150 volts and time variations of 30, 45, 60 minutes. Observation of electrophoresis results that have formed DNA bands will be measured the area of DNA bands using ImageJ application. Conclusion: Based on the results of the study concluded that: The optimum voltage in the electrophoresis process in obtaining good E.coli DNA bands is at a voltage of 150 volts. The optimum time in the electrophoresis process in obtaining good E.coli DNA bands is for 30 minutes.
PENGARUH PENYIMPANAN ARSIP BLOK PARAFIN TERHADAP KUALITAS PREPARAT JARINGAN Nurdianti, Dinda; Wiryanti, Wiwin; Durachim, Adang; Gustira Rahayu, Ira
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Paraffin block archive storage is the process of maintaining and managing used paraffin blocks. "A standardized paraffin block archive storage system will facilitate access for diagnostic purposes and help maintain the quality of the paraffin blocks over the long term. If the storage system is not standardized, it can cause several problems, such as delaying diagnostic services and reducing the quality of tissue preparations. Most paraffin blocks can be stored at temperatures of 20-27°C. Paraffin blocks should not be exposed to temperatures exceeding 27°C, as improper storage can cause the paraffin to soften and damage the tissue, reducing the quality of tissue preparations. This study aims to determine the effect of paraffin block archive storage on tissue preparation quality. The research samples used paraffin block archives stored in cabinets for 5 and 6 years, and paraffin block archives stored in plastic for 5 and 6 years. The quality of tissue preparations was assessed based on the clarity of the arrangement of bile duct cell walls, namely mucosa, lamina propria, muscularis and serosa microscopically and the color contrast of nuclei and cytoplasm using ImageJ software. The results of the study showed that there was no significant effect of paraffin block archive storage on tissue preparation quality. Further research needs to be conducted on the storage duration of paraffin block archives in wooden and plastic cabinets over a period of 10 years, as well as obtaining information related to the initial condition of the paraffin blocks, including color, texture, shape,and tissue authenticity.
PERBANDINGAN HASIL PEWARNAAN PREPARAT HISTOLOGI MENGGUNAKAN XYLOL DAN MINYAK JAGUNG YANG DIPANASKAN PADA PROSES DEPARAFINISASI Mufidah, Zahra; Wiryanti, Wiwin; Durachim, Adang; Rahmat, Mamat
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Deparaffinization removes residual paraffin from tissue, enabling optimal dye absorption in histological preparations. While xylol is commonly used, its high exposure risks necessitate exploring safer alternatives. The research compares the efficacy of corn oil 60°C to xylol as a deparaffinization agent. Heated corn oil is necessary to reduce viscosity and increase solubility. Corn oil can also be used as an environmentally friendly and cost-effective alternative. The research employed a descriptive approach, comparing staining results of kidney tissue preparations from control and experimental groups. Assessment criteria included color uniformity and contrast, determined by the difference in Optical Density values between nuclear and cytoplasmic staining. Due to non-normal data distribution and the comparison of two unpaired means, the Mann-Whitney U test was utilized for statistical analysis. The results obtained an Asymp sig value of 0.002 where the value is included <0.05 which explains that there is a significant different on the staining results of tissue preparations deparaffinized using xylol and corn oil 60°C with the percentage of staining results in the good category deparaffinized using corn oil only reached 26.67%. The results showed that there were differences in the staining results of kidney tissue preparations deparaffinized using xylol and 60°C corn oil so that 60°C corn oil has less effective to be an alternative deparaffinization agent to replace xylol.
PERBEDAAN KADAR KREATININ METODE JAFFE REACTION PADA SERUM SEGERA DAN SIMPAN SELAMA 1, 2, DAN 3 HARI PADA SUHU RUANG Salsabila Widayat, Niva
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

The laboratory examination stage consists of pre-analytical, analytical and post-analytical stages. One of the laboratory examination stages is the pre-analytical stage, where this stage has the largest error percentage, namely 77.1%. Checking creatinine levels is one of the tests to determine kidney function. This study aims to determine differences in serum creatinine levels immediately and stored for 1, 2 and 3 days at room temperature. This research was conducted in April 2024 at the Clinical Chemistry Laboratory, Technology Medical Laboratory, Health Polytechnic, Ministry of Health, Bandung. This research was quasi-experimental in nature by providing treatment in the form of storing serum after centrifugation for 1, 2, and 3 days at room temperature and then the levels were compared with the serum levels which were immediately examined. This examination was examined using the Jaffe reaction method using a photometer with a wave length of 505 nm and repeated in triplicate so that 72 data were obtained which were then processed using SPSS with the general linear model (GLM) test. The results of the study showed that the creatinine examination showed an average immediate serum value of 0.88 mg/dL and stored serum for 1 day 0.85 mg/dL, 2 days 0.81 mg/dL, 3 days 0.76 mg/dL (P < 0.05) which means there is a difference in the results of the immediate serum creatinine examination and the stored serum.
PERBANDINGAN LAMA WAKTU PENUNDAAN SPESIMEN FESES TERHADAP PEMERIKSAAN SOIL TRANSMITTED HELMINTH DENGAN METODE KATO−KATZ Jenita Nurrafikasari, Putri; Sulaeman; Sundara Mulia, Yuliansyah; Kurniawan, Entuy
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Soil transmitted helminth (STH) is a parasitic worm whose spread requires soil media. The spesieses of STH that infect humans are Ascaris lumbricoides, Trichuris trichiura, Necator americanus, and Ancylostoma duodenale. The Kato-katz method is easy to perform but requires accuracy in counting the number of worm eggs. The number of worm eggs found can be influenced by the length of time the sample is delayed. This research aims to determine the difference in the number of STH eggs based on the length of time delayed fresh feces samples in less than 3 hours and delayed feces samples less than 72 hours using the Kato-katz method. The research was conducted with feces samples from Padjadjaran University which indicated the presence of STH eggs. The results found in the delayed feces samples < 72 hours eggs are less than in fresh feces samples in < 3 hours. The number of Ascaris lumbricoides eggs in fresh samples in < 3 hours was 4,950 EPG while in samples delayed < 72 hours was 3,495 EPG. The number of Trichuris trichiura eggs in fresh samples in < 3 hours was 510 EPG while in samples delayed < 72 hours was 450 EPG. Based on Twoway Anova test obtained sig value. < 0.05 from the time delay to the results of the eggs found, namely 0.037. So, it can be interpreted that there is an influence in the length of time delayed examination of feces samples on the number of Soil transmitted helminth eggs found.
PERBANDINGAN METODE KATO – KATZ DENGAN METODE STOLL UNTUK PEMERIKSAAN TELUR CACING SOIL TRANSMITTED HELMINTH PADA SAMPEL FESES Octania nugraha, Hanayori; Sulaeman; Sundara, Yuliansyah; Kurniawan, Entuy
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Soil transmitted helminth (STH) is a group of intestinal parasitic worms of the nematode class that can cause human infection through soil contaminated with their eggs or larvae. Soil transmitted helminth types that most commonly infect humans are Ascaris lumbricoides (A. lumbricoides), Trichuris trichiura (T. trichiura) and hookworms (Necator americanus (N. americanus) and Ancylostoma duodenale (A. duodenale). The intended purpose of this study is to determine the similarity of worm egg identification results between the Kato-Katz and the Stoll method. The statistical analysis applied in this study is Paired T test. The results of this study showed that the Kato-Katz and Stoll methods applied to five feces samples indicated Soil Transmitted Helminth (STH) worms in the laboratory of the Poltekkes Kemenkes Bandung found the presence of the two most common types of worm eggs; Ascaris lumbricoides and Trichuris trichiura. Meanwhile, hookworm eggs were not found in the two methods. It was found that the number of Ascaris lumbricoides eggs was more when compared to Trichuris trichiura. Statistical data identified that both methods had a normal distribution (Shapiro-Wilk test) with a difference (Paired T_test, p=0.024). Where the most eggs were found in the Kato-Katz method. The Kato-Katz and Stoll methods for Trichuris trichiura had a correlation (r=0.893, p=0.0001). The sensitivity of these two methods is 100%, but the specificity cannot be calculated because all samples were positive for STH. Therefore, WHO recommends the Kato-Katz method in the examination of STH infection because it is superior in the detection of worm eggs.
LAPORAN KASUS BERBASIS BUKTI: PENGARUH KOMPRES AIR HANGAT TERHADAP PENGURANGAN NYERI PERSALINAN KALA I FASE AKTIF Lestari, Anastasya Deby; Wisnu Wardhani, Sri
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Labor pain is a common complaint experienced by women during childbirth, especially during the active phase of the first stage of labor. Previous research has shown that primiparous women tend to experience more intense pain compared to multiparous women. Objective: This study aims to evaluate the effectiveness of warm water compresses in reducing the intensity of labor pain during the active phase of the first stage of labor in women giving birth at UPTD Puskesmas Garuda. Methods: The study design used a case study of women in the active phase of the first stage of labor. The variables observed were the intensity of pain before and after the application of warm water compresses. Results: The results of the case study showed a significant decrease in pain intensity after the application of warm water compresses. Conclusion: Warm water compresses were proven to be effective in reducing the intensity of labor pain during the active phase of the first stage of labor in women giving birth at UPTD Puskesmas Garuda. Implications: These findings can be a clinical recommendation for healthcare workers, especially midwives, to use warm water compresses as one of the non-pharmacological methods for managing labor pain.
Pengaruh Edukasi Prakonsepsi terhadap Perilaku Premarital Check Up pada Calon Pengantin di Kota Bandung Nezima, Zilca
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Background: The postpartum period is a physiological thing experienced by every mother after giving birth and has an impact on physiological and psychological changes in postpartum mothers. One of the most common complaints experienced by postpartum mothers is fatigue. Symptoms of fatigue occur in around 64% of mothers in the postpartum period. It is reported that 75% of 10 postpartum mothers experience fatigue such as frequent yawning, stiff backs, unbalanced when standing and 25% say mothers have difficulty concentrating. Non-pharmacological therapy is needed to reduce fatigue in postpartum mothers, one of which is the effleurage hand massage technique with lavender aromatherapy. The combination of aromatherapy and massage in postpartum mothers can reduce the fatigue felt by the mother. Purpose:   To determine the effectiveness of effleurage hand massage with lavender aromatherapy to reduce fatigue in postpartum mothers. Method: Case study on postpartum mothers using e-data based search engines Pubmed and Google Scholar with publication years 2017-2024. Result: After effleurage hand massage with lavender aromatherapy, the mother experienced mild fatigue. Conclusion: Effleurage hand massage with lavender aromatherapy is effective in reducing fatigue in postpartum mothers. 
EVIDENCE BASED CASE REPORT (EBCR) : PENGARUH PEMBERIAN PIJAT OKETANI TERHADAP PRODUKSI ASI Khoirul Barriyah, Jasmine; Sofiyanti, Santi; Resmana, Rika
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Background: Breastfeeding is a nutritional need for babies during growth and development. Breast milk (ASI) helps in sensory, cognitive, and motor development and protects and prevents babies from various diseases and infections. Breast milk production is influenced by the hormones prolactin and oxytocin, food intake, the mother's psychological condition, breast care, and the frequency of breastfeeding. In stimulating the release of these hormones, one of the efforts made is breast care. Breast care will stimulate the breasts and affect the hypopise to release more progesterone, estrogen, and oxytocin hormones. The hormone oxytocin will contract other cells around the alveoli, causing milk to flow down towards the nipple. One of the breast care techniques is oketani massage. This massage is a massage technique popularized in Japan that will make the breasts softer, the areola and nipples elastic, making it easier for babies to suckle breast milk. Objective: To determine the effect of oketani massage on postpartum maternal breast milk production. Method: This journal search uses keywords, Boolean Operators and E-data based with literature review and experimental research types. Results: Based on this study, it was found that there was an effect of Oketani massage on breast milk production in postpartum mothers. Conclusion: It is expected that health workers can use the Oketani massage technique to help breast milk production in postpartum mothers as an easy and inexpensive alternative that can help mothers in breast milk production.
EVIDENCE BASED CASE REPORT (EBCR): PENGARUH HIPNOBIRTHING TERHADAP PENURUNAN NYERI PERSALINAN KALA 1 FASE AKTIF Fadhilah, Chagya; Pascawati, Riana; Ulfah Fatimah, Yulia
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Background: Labor pain is a physiological process that occurs during labour, where individual responses to pain vary. So it requires efforts to overcome labor pain using pharmacological and non-pharmacological methods, one of which is the hypnobirthing method. Purpose: This application aims to provide a positive experience for mothers during labour with a sense of comfort and calm during the labour process. Method: The literature e-database PubMed, Cochrane, Science Direct and Google Scholar was used to search for evidence. Results: In this case, the results showed changes in labour pain using VAS measurements. Discussion: Hypnobirthing works by activating nerves in the brain, causing the release of natural morphine-like substances called enkephalins and endorphins. So that it can increase the mother's tolerance to pain. Conclusion: Hypnobirthing method affect reducing labour pain.

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