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INDONESIA
Jurnal Kesehatan Siliwangi
Published by Politeknik Kesehatan Kemenkes Bandung
ISSN : -     EISSN : 28071530     DOI : https://doi.org/10.34011/jks.v4i1.1671
Core Subject : Health, Science, Social,
Biochemistry, Genetics & Molecular Biology Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology
Jurnal Kesehatan Siliwangi menerima publikasi dalam makalah penelitian artikel asli, makalah tinjauan, laporan kasus, pengabdian masyarakat, penelitian kualitatif/dan kuantitatif di Indonesia atau negara lain untuk memberikan pemahaman mengenai aspek kesehatan. Ruang lingkup Artikel yang diterbitkan dalam jurnal ini adalah bidang : - Analis Kesehatan/ Teknik Laboratorium Medik - Gizi - Farmasi - Keperawatan Gigi - Keperawatan - Kebidanan - Kesehatan Lingkungan - Promosi Kesehatan
Arjuna Subject : Kedokteran - Kesehatan Masyarakat, Kesehatan Lingkungan dan Pekerjaan
Articles 532 Documents
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PERBEDAAN SUHU DAN WAKTU PENYIMPANAN SPESIMEN SPUTUM TERHADAP HASIL PEMERIKSAAN MIKROSKOPIS BAKTERI TAHAN ASAM Alfi Agilia Saputri, Nianda; Ilmi Sufa , Hafizah; Kurniati, Iis; Dermawan, Asep
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Sputum specimens should ideally be examined immediately to ensure accurate BTA (Acid Resistant Bacteria) microscopic examination results. The limited number of ATLMs while the large amount of sputum causes delays in examination. The purpose of the study was to determine the differences in characteristics and the number of BTA based on the International Union Againtst Tuberculosis and Lung Disease (IUATLD) scale of sputum examined immediately with those stored at temperatures (23-27°C) and (35-39°C) with storage times of 6.5 and 7.5 hours. The research design used was quasi-experimental. The results showed there were changes in the characteristics of sputum stored at temperatures (23-27°C) and (35-39°C) with storage times of 6.5 and 7.5 hours against sputum examined immediately. Sputum examined immediately had a BTA count of 1+. Sputum stored at temperature (23-27°C) at storage times of 6.5 and 7.5 hours the number of BTA is 1+, while sputum stored at temperature (35-39°C) at storage times of 6.5 and 7.5 hours the number of BTA is 2+. So it can be concluded that there are changes in the characteristics of sputum stored at temperatures (23-27°C) and (35-39°C) with storage times of 6.5 and 7.5 hours. There is no difference in the number of BTA scale (IUATLD) of sputum stored at temperature (23-27°C) at storage times of 6.5 and 7.5 hours, while sputum stored at temperature (35-39°C) at storage times of 6.5 and 7.5 hours there is a difference in the number of BTA scale (IUATLD) of sputum specimens examined immediately.
EFEKTIVITAS KONSENTRASI DAN WAKTU MASERASI EKSTRAK DAUN BELUNTAS (Pluchea indica L.) TERHADAP PERTUMBUHAN Streptococcus pyogenes Ismi Yulandari, Siti; Dermawan, Asep; Kurniati, Iis; Iin Nur Indra, Asep
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

One of the most common diseases in Indonesia, namely pharyngitis, is caused by Streptococcus pyogenes. Inappropriate treatment of pharyngitis with antibiotics can result in bacteria that are resistant to antibacterials. Therefore, herbal plants that are effective as natural antibacterials are needed, such as beluntas leaves (Pluchea indica L.) to prevent this. Apart from that, the right method is needed so that the contents of the beluntas leaves can be attracted properly. The aim of this research was to determine the effective concentration and maceration time of beluntas leaf extract in inhibiting the growth of S.pyogenes. This research used varying maceration times of 24 and 72 hours. Then, from each variation of maceration time, beluntas leaf extract was made in concentrations of 5%, 10%, 15%, 20%, and 25%.  Beluntas leaf extract was tested for its inhibitory power against S.pyogenes using the Kirby Bauer method. The data obtained was the diameter of the inhibitory power of beluntas leaf extract on the growth of S.pyogenes, then the data was processed statistically using the Kruskal-Wallis test and a further test, namely the Post Hoc Test. The results of this research were that beluntas leaf extract which was macerated for 72 hours with a concentration of 20% and 25% had an average diameter of inhibition against S.pyogenes of 9.04 mm and 12.71 mm. Therefore, a maceration time of 72 hours with a concentration of 25% is effective in inhibiting the growth of S.pyogenes.
OPTIMASI WAKTU INKUBASI VAKSIN H5N1 PADA TELUR AYAM BEREMBRIO DENGAN SUHU 37OC TERHADAP TITER HEMAGLUTINASI Fuji Lestary, Rizka; Kurniati, Iis; Dermawan, Asep; Riyani, Ani
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Avian Influenza virus subtype H5N1 can infect animals as well as humans due to its high pathogenicity. Avian Influenza virus contains hemagglutinin protein that can agglutinate red blood cells. The purpose of this study was to determine the optimal incubation time, namely 3 days, 4 days, and 5 days and whether there are differences in the different incubation times. The type of research used was descriptive. The samples used were Avian Influenza Vaccine subtype H5N1 inoculated into embryonated chicken eggs aged 9-11 days. The allantois fluid produced from vaccine inoculation in embryonated chicken eggs can be tested for hemagglutination to determine its titer. The results showed that the optimum incubation time was 3 days and the optimum hemagglutination titer at the 3rd day incubation time was 1/256, at the 4th day incubation time was 1/256, and at the 5th day incubation time was 1/512. The data were analyzed using One-Way Anova test on SPSS. In conclusion, there was a significant difference in hemagglutination titer in the H5N1 vaccine inoculated in embryonated chicken eggs for 3 days and 4 days against the incubation time for 5 days.
EFEKTIVITAS SUHU DAN WAKTU PENYIMPANAN AIR MINUM ISI ULANG TERHADAP HASIL MOST PROBABLE NUMBER (MPN) COLIFORM Zahra Amalia, Farah
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Refillable drinking water depots have currently become the most widely chosen source of water by the community due to their relatively affordable prices and easy accessibility around residential areas. However, the selection of these refillable drinking water depots can pose risks that can endanger health. Although they have undergone strict processing and packaging, there is a possibility that refillable drinking water can be contaminated by Coliform. One of the factors that can affect the level of Coliform contamination in refillable drinking water is the storage temperature and time.This study aims to determine the effectiveness of storage temperature and time of refillable drinking water on the results of the Most Probable Number (MPN) Coliform test. The type of research used is Quasi-Experiment. Refillable drinking water samples were taken from 30 houses around SMAN 1 Kota Sukabumi. The samples were divided into 2 storage temperature treatments (25°-30°C and 70°-80°C) with storage times of 3 days. The results showed that there was a significant influence between temperature and storage time treatments on the MPN Coliform results of refillable drinking water. The 70°-80°C temperature treatment with 1, 2, and 3 days of storage time resulted in the lowest MPN Coliform, while the 25°-30°C treatment with an average of 27.10°C and 3 days of storage time resulted in the highest MPN Coliform. It can be concluded that the storage of refillable drinking water at a temperature of 27.10°C for 3 days is the most effective treatment in determining the MPN Coliform.
STABILITAS REAKSI ENZIMATIK KADAR TRIGLISERIDA SERUM METODE Glyserol Phosphate Oxidase Para-Aminoantipyrin (GPO-PAP) DENGAN VARIASI WAKTU INKUBASI Endrique Putri, Salmanda; Kurnaeni, Nani; Nurhayati, Dewi; Merdekawati, Fusvita
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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One of the triglycerides test lipid profile examination that is often analyzed because it provides information about cardiovascular health and stroke. Laboratory services have an important meaning in making a diagnosis based on the patient's health history and are an integral part in achieving quality laboratory results that have accurate accuracy and precision. Analytical factors that can influence examination results include incubation time. The delay in the length of incubation time is due to the limited number of laboratory personnel and inadequate equipment. The objective of this study was to assess the impact of stability on serum triglyceride levels using the GPO-PAP method with different incubation durations. The research employed a quasi-experimental design, utilizing serum samples from three Medical Laboratory Technology students. The samples were subjected to varying incubation times of 10, 60, 75, 90, and 105 minutes at room temperature, with each condition being repeated five times. The mean results of serum triglyceride levels with incubation times of 10, 60, 75, 90, and 105 minutes respectively were 80,0 mg/dL, 79,00 mg/dL, 76.36 mg/dL, 73.65 mg/ dL, and 67.94 mg/dL. Data analysis was conducted using the General Linear Model (GLM) test, with significance indicated by the Sig value. < 0.05 means that triglyceride levels with an incubation time of 75 minutes are statistically unstable.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN CENGKEH (Syzygium aromaticum L.) TERHADAP Streptococcus pyogenes Ghaisani, Syifa Marha; Kurniati, Iis; Ilmi Sufa, Hafizah; Rahmat, Mamat
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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One of the most common bacterial infections is pharyngitis. Most cases of acute pharyngitis are caused by viruses, but the main bacterial agent is Streptococcus pyogenes. Some antibiotics have been resistant to Streptococcus pyogenes, so alternative antibacterials are needed, namely the use of clove leaves (Syzygium aromaticum L.). The type of research used is Quasi Experiment. This study was conducted to test the antibacterial activity of clove leaves against Streptococcus pyogenes. Clove leaf extract was obtained by maceration method using 96% ethanol solvent. Phytochemical tests on clove leaf extracts showed positive leaves containing saponins, phenols, terpenoids, flavonoids, and tannins. Antibacterial activity test was conducted by disc diffusion method with variation of extract concentration of 10%, 15%, and 25% and incubation time for 24 and 48 hours. Bacitracin disc was used as positive control and negative control used 10% DMSO. The test results showed that there was antibacterial activity indicated by the formation of inhibition zone at 25% concentration with an average of 9.23 mm and 15% concentration of 8.8 mm. The zone of inhibition formed did not match the zone of inhibition in the control (≥13 mm).
POTENSI EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb) DALAM MENGHAMBAT PERTUMBUHAN Streptococcus pyogenes Nurdini, Lia; Dermawan, Asep; Ilmi Sufa, Hafizah; Rohayati
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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S. pyogenes is the primary cause of acute pharyngitis. The inappropriate, repeated, and irregular use of antibiotics can lead to resistance. One alternative is to utilize plants containing antibacterial compounds. This study aims to identify the concentration and contact time of fragrant pandan leaf extract that has the potential to inhibit the growth of S. pyogenes. The method applied in this study is the disk diffusion test with fragrant pandan leaf extract at concentrations of 20%, 40%, and 60%, with contact periods of 24 hours and 48 hours. Bacitracin was used as a positive control, and 10% DMSO as a negative control. The data were analyzed using a Two Way ANOVA test, showing a significant difference between the extract concentration and contact time on the inhibition zone diameter (p<0.05). The study resulted in an average inhibition zone diameter at a 20% concentration of 9 mm after 24 hours, 40% at 9.9 mm, and 60% at 11 mm. For the 48-hour contact time, the average inhibition zone diameter at a 20% concentration was 8.9 mm, 40% was 9.7 mm, and 60% was 10.8 mm. The average inhibition zone diameter in the positive control (bacitracin) was 18.3 mm, while the negative control showed no inhibition zone.
PERBANDINGAN PEMERIKSAAN TINJA ANTARA METODE FLOTASI DAN METODE RITCHIE DALAM MENDETEKSI SOIL TRANSMITTED HELMINTH Wasilah, Nayla; Sulaeman; Sundara Mulia, Yuliansyah; Firman Solihat, Mohamad
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
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Microscopic stool examination has two examinations, namely qualitative and quantitative examination. Methods that can be done for qualitative fecal examination are direct slide method, anal swab method, flotation method, tape method, and sedimentation method. While in quantitative examination, namely Stoll, Ritchie, and Kato Katz. This study aims to determine the difference in the identification results of the number of eggs of each species found in the Flotation Method and the Ritchie Method. This research was conducted at the Parasitology Laboratory of the Polytechnic of the Ministry of Health Bandung in March-April 2024. The population in this study was obtained from feces suspected of Soil Transmitted Helminth. The number of samples used in this study were 30 samples using purposive sampling method, namely sampling techniques with certain criteria. Data analysis used the Paired Sample T Test statistical test by calculating the sensitivity and specificity values. The results showed that the number of Soil Transmitted Helminth eggs was found more in the ritchie method, namely in Ascaris lumbricoides 350 (84.95%) and Trichuris trichiura 62 (15.05%), while in the flotation method in Ascaris lumbricoides 195 (85.15%) and Trichuris trichiura 34 (14.85%). Flotation method and Ritchie method have the same sensitivity and specificity values, namely 100% sensitivity and 0% specificity. The results of the Paired Sample T Test have a significant value of 0.001 (p < 0.05) indicating that there is a difference in the results of STH examination with flotation and ritchie methods.
OPTIMASI MEDIA ALTERNATIF NUTRIENT AGAR BERBAHAN KACANG MERAH (Phaseolus vulgaris L.) DALAM MENUMBUHKAN Staphylococcus aureus Mutiara Sulam, Nuri; Ilmi Sufa, Hafizah; Dermawan, Asep; Rismiati, Zuri
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
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Media are used to grow bacteria to diagnose infectious diseases, stock bacterial cultures, and in educational institutes for learning and research on the characteristic of bacteria. One of these media is nutrient agar which contains nutrients in the form of protein and carbohydrates. Growth media has less affordable prices so alternative media are created. Alternative media to replace nutrient agar with a vegetable protein source to replace the protein contained in the media. For example, the vegetable protein used is from red beans. Red beans contain protein and amino acids which will replace protein in nutrient agar media. This research aims to determine alternative media made from red beans that can be used as a substitute for nutrient agar, the optimum concentration and boiling time of red beans in growing Staphylococcus aureus with concentration variations of 5%, 10%, and 15% and time variations of 5 minutes, 10 minutes, and 15 minutes. In the research, inoculation was carried out using the pouring cup method. The results of the number of colonies on the alternative media will be compared with the results of the number of colonies on the control, namely nutrient agar. Based on the Generalized Linear Model (GLM) statistical test, an alternative media with a concentration of 10% and a boiling time of 10 minutes can be used as a substitute for nutrient agar.
Comparison of Color Quality in Tissue Preparations using Methyl Ester Sulfonate And Xylol as a Deparafinization Agent Rahmah, Syafa Aulia; Adang Durachim; Wiwin Wiryanti; Asep Iin Nur Indra
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
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Deparaffinization is the process of decaying the remnants of paraffin in tissue preparations. Deparaffinization aims to make the dye that will be given to the preparation fully absorbed so that the preparation can be colored properly. This deparaffinization process uses xylol solution in its immersion. Methyl Ester Sulfonate (MES) is one of the vegetable oil-based anionic surfactants that can dissolve in water and has a long carbon chain that is soluble in oil and vaseline. The purpose of this study was to determine whether Methyl Ester Sulfonate can be used as a deparaffinizing agent for tissue preparations. The sample in this study was appendiceal tissue as many as 30 units, each unit of tissue made into 2 tissue preparations so that it became 60 tissue preparations, namely 30 tissues using xylol as a deparaffinizing agent and 30 tissues using methyl ester sulfonate. The parameters of this study were the clarity of the color of the nucleus and cytoplasm, the uniformity of cell color, and the contrast of the color of the nucleus and cytoplasm qualitatively and quantitatively. The statistical test used was Independent T-Test test if the data were normally distributed and Mann Whitney test if the data were not normally distributed. Based on the Independent T-Test test, the Sig value was obtained. 0.951 and 0.102, both of which are >0.05, meaning that there is no significant difference between the use of xylol and 10% methyl ester sulfonate as a deparaffinizing agent.

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