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Jurnal Kesehatan Siliwangi
Published by Politeknik Kesehatan Kemenkes Bandung
ISSN : -     EISSN : 28071530     DOI : https://doi.org/10.34011/jks.v4i1.1671
Core Subject : Health, Science, Social,
Biochemistry, Genetics & Molecular Biology Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology
Jurnal Kesehatan Siliwangi menerima publikasi dalam makalah penelitian artikel asli, makalah tinjauan, laporan kasus, pengabdian masyarakat, penelitian kualitatif/dan kuantitatif di Indonesia atau negara lain untuk memberikan pemahaman mengenai aspek kesehatan. Ruang lingkup Artikel yang diterbitkan dalam jurnal ini adalah bidang : - Analis Kesehatan/ Teknik Laboratorium Medik - Gizi - Farmasi - Keperawatan Gigi - Keperawatan - Kebidanan - Kesehatan Lingkungan - Promosi Kesehatan
Arjuna Subject : Kedokteran - Kesehatan Masyarakat, Kesehatan Lingkungan dan Pekerjaan
Articles 546 Documents
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STABILITAS REAKSI ENZIMATIK KADAR TRIGLISERIDA SERUM METODE Glyserol Phosphate Oxidase Para-Aminoantipyrin (GPO-PAP) DENGAN VARIASI WAKTU INKUBASI Endrique Putri, Salmanda; Kurnaeni, Nani; Nurhayati, Dewi; Merdekawati, Fusvita
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

One of the triglycerides test lipid profile examination that is often analyzed because it provides information about cardiovascular health and stroke. Laboratory services have an important meaning in making a diagnosis based on the patient's health history and are an integral part in achieving quality laboratory results that have accurate accuracy and precision. Analytical factors that can influence examination results include incubation time. The delay in the length of incubation time is due to the limited number of laboratory personnel and inadequate equipment. The objective of this study was to assess the impact of stability on serum triglyceride levels using the GPO-PAP method with different incubation durations. The research employed a quasi-experimental design, utilizing serum samples from three Medical Laboratory Technology students. The samples were subjected to varying incubation times of 10, 60, 75, 90, and 105 minutes at room temperature, with each condition being repeated five times. The mean results of serum triglyceride levels with incubation times of 10, 60, 75, 90, and 105 minutes respectively were 80,0 mg/dL, 79,00 mg/dL, 76.36 mg/dL, 73.65 mg/ dL, and 67.94 mg/dL. Data analysis was conducted using the General Linear Model (GLM) test, with significance indicated by the Sig value. < 0.05 means that triglyceride levels with an incubation time of 75 minutes are statistically unstable.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN CENGKEH (Syzygium aromaticum L.) TERHADAP Streptococcus pyogenes Ghaisani, Syifa Marha; Kurniati, Iis; Ilmi Sufa, Hafizah; Rahmat, Mamat
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

One of the most common bacterial infections is pharyngitis. Most cases of acute pharyngitis are caused by viruses, but the main bacterial agent is Streptococcus pyogenes. Some antibiotics have been resistant to Streptococcus pyogenes, so alternative antibacterials are needed, namely the use of clove leaves (Syzygium aromaticum L.). The type of research used is Quasi Experiment. This study was conducted to test the antibacterial activity of clove leaves against Streptococcus pyogenes. Clove leaf extract was obtained by maceration method using 96% ethanol solvent. Phytochemical tests on clove leaf extracts showed positive leaves containing saponins, phenols, terpenoids, flavonoids, and tannins. Antibacterial activity test was conducted by disc diffusion method with variation of extract concentration of 10%, 15%, and 25% and incubation time for 24 and 48 hours. Bacitracin disc was used as positive control and negative control used 10% DMSO. The test results showed that there was antibacterial activity indicated by the formation of inhibition zone at 25% concentration with an average of 9.23 mm and 15% concentration of 8.8 mm. The zone of inhibition formed did not match the zone of inhibition in the control (≥13 mm).
POTENSI EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb) DALAM MENGHAMBAT PERTUMBUHAN Streptococcus pyogenes Nurdini, Lia; Dermawan, Asep; Ilmi Sufa, Hafizah; Rohayati
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

S. pyogenes is the primary cause of acute pharyngitis. The inappropriate, repeated, and irregular use of antibiotics can lead to resistance. One alternative is to utilize plants containing antibacterial compounds. This study aims to identify the concentration and contact time of fragrant pandan leaf extract that has the potential to inhibit the growth of S. pyogenes. The method applied in this study is the disk diffusion test with fragrant pandan leaf extract at concentrations of 20%, 40%, and 60%, with contact periods of 24 hours and 48 hours. Bacitracin was used as a positive control, and 10% DMSO as a negative control. The data were analyzed using a Two Way ANOVA test, showing a significant difference between the extract concentration and contact time on the inhibition zone diameter (p<0.05). The study resulted in an average inhibition zone diameter at a 20% concentration of 9 mm after 24 hours, 40% at 9.9 mm, and 60% at 11 mm. For the 48-hour contact time, the average inhibition zone diameter at a 20% concentration was 8.9 mm, 40% was 9.7 mm, and 60% was 10.8 mm. The average inhibition zone diameter in the positive control (bacitracin) was 18.3 mm, while the negative control showed no inhibition zone.
PERBANDINGAN PEMERIKSAAN TINJA ANTARA METODE FLOTASI DAN METODE RITCHIE DALAM MENDETEKSI SOIL TRANSMITTED HELMINTH Wasilah, Nayla; Sulaeman; Sundara Mulia, Yuliansyah; Firman Solihat, Mohamad
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Microscopic stool examination has two examinations, namely qualitative and quantitative examination. Methods that can be done for qualitative fecal examination are direct slide method, anal swab method, flotation method, tape method, and sedimentation method. While in quantitative examination, namely Stoll, Ritchie, and Kato Katz. This study aims to determine the difference in the identification results of the number of eggs of each species found in the Flotation Method and the Ritchie Method. This research was conducted at the Parasitology Laboratory of the Polytechnic of the Ministry of Health Bandung in March-April 2024. The population in this study was obtained from feces suspected of Soil Transmitted Helminth. The number of samples used in this study were 30 samples using purposive sampling method, namely sampling techniques with certain criteria. Data analysis used the Paired Sample T Test statistical test by calculating the sensitivity and specificity values. The results showed that the number of Soil Transmitted Helminth eggs was found more in the ritchie method, namely in Ascaris lumbricoides 350 (84.95%) and Trichuris trichiura 62 (15.05%), while in the flotation method in Ascaris lumbricoides 195 (85.15%) and Trichuris trichiura 34 (14.85%). Flotation method and Ritchie method have the same sensitivity and specificity values, namely 100% sensitivity and 0% specificity. The results of the Paired Sample T Test have a significant value of 0.001 (p < 0.05) indicating that there is a difference in the results of STH examination with flotation and ritchie methods.
OPTIMASI MEDIA ALTERNATIF NUTRIENT AGAR BERBAHAN KACANG MERAH (Phaseolus vulgaris L.) DALAM MENUMBUHKAN Staphylococcus aureus Mutiara Sulam, Nuri; Ilmi Sufa, Hafizah; Dermawan, Asep; Rismiati, Zuri
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Media are used to grow bacteria to diagnose infectious diseases, stock bacterial cultures, and in educational institutes for learning and research on the characteristic of bacteria. One of these media is nutrient agar which contains nutrients in the form of protein and carbohydrates. Growth media has less affordable prices so alternative media are created. Alternative media to replace nutrient agar with a vegetable protein source to replace the protein contained in the media. For example, the vegetable protein used is from red beans. Red beans contain protein and amino acids which will replace protein in nutrient agar media. This research aims to determine alternative media made from red beans that can be used as a substitute for nutrient agar, the optimum concentration and boiling time of red beans in growing Staphylococcus aureus with concentration variations of 5%, 10%, and 15% and time variations of 5 minutes, 10 minutes, and 15 minutes. In the research, inoculation was carried out using the pouring cup method. The results of the number of colonies on the alternative media will be compared with the results of the number of colonies on the control, namely nutrient agar. Based on the Generalized Linear Model (GLM) statistical test, an alternative media with a concentration of 10% and a boiling time of 10 minutes can be used as a substitute for nutrient agar.
Comparison of Color Quality in Tissue Preparations using Methyl Ester Sulfonate And Xylol as a Deparafinization Agent Rahmah, Syafa Aulia; Adang Durachim; Wiwin Wiryanti; Asep Iin Nur Indra
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Deparaffinization is the process of decaying the remnants of paraffin in tissue preparations. Deparaffinization aims to make the dye that will be given to the preparation fully absorbed so that the preparation can be colored properly. This deparaffinization process uses xylol solution in its immersion. Methyl Ester Sulfonate (MES) is one of the vegetable oil-based anionic surfactants that can dissolve in water and has a long carbon chain that is soluble in oil and vaseline. The purpose of this study was to determine whether Methyl Ester Sulfonate can be used as a deparaffinizing agent for tissue preparations. The sample in this study was appendiceal tissue as many as 30 units, each unit of tissue made into 2 tissue preparations so that it became 60 tissue preparations, namely 30 tissues using xylol as a deparaffinizing agent and 30 tissues using methyl ester sulfonate. The parameters of this study were the clarity of the color of the nucleus and cytoplasm, the uniformity of cell color, and the contrast of the color of the nucleus and cytoplasm qualitatively and quantitatively. The statistical test used was Independent T-Test test if the data were normally distributed and Mann Whitney test if the data were not normally distributed. Based on the Independent T-Test test, the Sig value was obtained. 0.951 and 0.102, both of which are >0.05, meaning that there is no significant difference between the use of xylol and 10% methyl ester sulfonate as a deparaffinizing agent.
OPTIMASI VARIASI VOLTASE DAN WAKTU TERHADAP KUALITAS PITA DNA ESCHERICHIA COLI PADA PROSES ELEKTROFORESIS GEL AGAROSA nur amani putri, afifah; Iin Nur Indra, Asep; Merdekawati, Fusvita; Khoirul Abror, Yogi
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Background: Escherichia coli is one of the causes of foodborne illness. Conventional PCR is a PCR method that is carried out qualitatively followed by visualization on agar electrophoresis. Agarose gel electrophoresis is a technique that is often used in various fields of science to separate a mixture of DNA on agarose substrate. This method is used to perform qualitative analysis of DNA samples. In electrophoresis, there are factors that affect the movement of DNA molecules, one of which is voltage. In addition, the length of time of the electrophoresis process can also affect the effectiveness of the results and the rate of migration. When the electrophoresis process time is short, large DNA fragments still tend to stick. Purpose: Therefore, it is necessary to balance the voltage and time given to get good DNA banding results. Methods: The research unit that will be used is the result of amplification of Escherichia coli 16SrRNA gene DNA measuring 584 bp. The electrophoresis process was carried out with voltage variations of 50, 100, 150 volts and time variations of 30, 45, 60 minutes. Observation of electrophoresis results that have formed DNA bands will be measured the area of DNA bands using ImageJ application. Conclusion: Based on the results of the study concluded that: The optimum voltage in the electrophoresis process in obtaining good E.coli DNA bands is at a voltage of 150 volts. The optimum time in the electrophoresis process in obtaining good E.coli DNA bands is for 30 minutes.
PENGARUH PENYIMPANAN ARSIP BLOK PARAFIN TERHADAP KUALITAS PREPARAT JARINGAN Nurdianti, Dinda; Wiryanti, Wiwin; Durachim, Adang; Gustira Rahayu, Ira
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Paraffin block archive storage is the process of maintaining and managing used paraffin blocks. "A standardized paraffin block archive storage system will facilitate access for diagnostic purposes and help maintain the quality of the paraffin blocks over the long term. If the storage system is not standardized, it can cause several problems, such as delaying diagnostic services and reducing the quality of tissue preparations. Most paraffin blocks can be stored at temperatures of 20-27°C. Paraffin blocks should not be exposed to temperatures exceeding 27°C, as improper storage can cause the paraffin to soften and damage the tissue, reducing the quality of tissue preparations. This study aims to determine the effect of paraffin block archive storage on tissue preparation quality. The research samples used paraffin block archives stored in cabinets for 5 and 6 years, and paraffin block archives stored in plastic for 5 and 6 years. The quality of tissue preparations was assessed based on the clarity of the arrangement of bile duct cell walls, namely mucosa, lamina propria, muscularis and serosa microscopically and the color contrast of nuclei and cytoplasm using ImageJ software. The results of the study showed that there was no significant effect of paraffin block archive storage on tissue preparation quality. Further research needs to be conducted on the storage duration of paraffin block archives in wooden and plastic cabinets over a period of 10 years, as well as obtaining information related to the initial condition of the paraffin blocks, including color, texture, shape,and tissue authenticity.
PERBANDINGAN HASIL PEWARNAAN PREPARAT HISTOLOGI MENGGUNAKAN XYLOL DAN MINYAK JAGUNG YANG DIPANASKAN PADA PROSES DEPARAFINISASI Mufidah, Zahra; Wiryanti, Wiwin; Durachim, Adang; Rahmat, Mamat
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
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Deparaffinization removes residual paraffin from tissue, enabling optimal dye absorption in histological preparations. While xylol is commonly used, its high exposure risks necessitate exploring safer alternatives. The research compares the efficacy of corn oil 60°C to xylol as a deparaffinization agent. Heated corn oil is necessary to reduce viscosity and increase solubility. Corn oil can also be used as an environmentally friendly and cost-effective alternative. The research employed a descriptive approach, comparing staining results of kidney tissue preparations from control and experimental groups. Assessment criteria included color uniformity and contrast, determined by the difference in Optical Density values between nuclear and cytoplasmic staining. Due to non-normal data distribution and the comparison of two unpaired means, the Mann-Whitney U test was utilized for statistical analysis. The results obtained an Asymp sig value of 0.002 where the value is included <0.05 which explains that there is a significant different on the staining results of tissue preparations deparaffinized using xylol and corn oil 60°C with the percentage of staining results in the good category deparaffinized using corn oil only reached 26.67%. The results showed that there were differences in the staining results of kidney tissue preparations deparaffinized using xylol and 60°C corn oil so that 60°C corn oil has less effective to be an alternative deparaffinization agent to replace xylol.
PERBEDAAN KADAR KREATININ METODE JAFFE REACTION PADA SERUM SEGERA DAN SIMPAN SELAMA 1, 2, DAN 3 HARI PADA SUHU RUANG Salsabila Widayat, Niva
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
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The laboratory examination stage consists of pre-analytical, analytical and post-analytical stages. One of the laboratory examination stages is the pre-analytical stage, where this stage has the largest error percentage, namely 77.1%. Checking creatinine levels is one of the tests to determine kidney function. This study aims to determine differences in serum creatinine levels immediately and stored for 1, 2 and 3 days at room temperature. This research was conducted in April 2024 at the Clinical Chemistry Laboratory, Technology Medical Laboratory, Health Polytechnic, Ministry of Health, Bandung. This research was quasi-experimental in nature by providing treatment in the form of storing serum after centrifugation for 1, 2, and 3 days at room temperature and then the levels were compared with the serum levels which were immediately examined. This examination was examined using the Jaffe reaction method using a photometer with a wave length of 505 nm and repeated in triplicate so that 72 data were obtained which were then processed using SPSS with the general linear model (GLM) test. The results of the study showed that the creatinine examination showed an average immediate serum value of 0.88 mg/dL and stored serum for 1 day 0.85 mg/dL, 2 days 0.81 mg/dL, 3 days 0.76 mg/dL (P < 0.05) which means there is a difference in the results of the immediate serum creatinine examination and the stored serum.

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