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Hartalina Mufidah
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Journal of Medical Laboratory in Infectious and Degenerative Diseases
Published by Universitas dr. Soebandi
ISSN : -     EISSN : 30325447     DOI : https://doi.org/10.36858/jmid.v1i2
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Public Health
Journal of Medical Laboratory in Infectious and Degenerative Diseases is a scientific journal with the scope of research conducted by lecturers, practitioners, and students. The purpose of publishing this journal is to accommodate scientific writings that have the substance of research activities, which are the downstream results of research carried out based on an analysis of the needs of the community for scientific and technological contributions in the fields of Parasitology, Bacteriology, Virology, Hematology, Clinical Chemistry, Immunology, Molecular Biology, Cytohistotechnology, Micology, Clinical Toxicology, and Degenerative Diseases.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 5 Documents
 1 
Search results for , issue "Vol. 3 No. 1 (2025): Juni" : 5 Documents clear
Evaluation of Liver Function Through SGOT and SGPT Quantification in Rats Administered Polyethylene Terephthalate Microplastics Nadhifah, Diana; Mufidah, Hartalina; Imroatul Muflihah, Ahdiah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.29

Abstract

Background: Microplastics, as emerging environmental pollutants, can accumulate in target organs such as the liver and induce hepatotoxicity through oxidative stress mechanisms. Purpose: To determine the effect of PET microplastic exposure at various doses on liver transaminase enzyme levels (SGOT and SGPT) in white rats. Methods: This experimental study used 25 male white rats (Rattus norvegicus), aged 8–10 weeks, divided into five groups (n=5 per group): control (distilled water), P1 (0.4 mg/day), P2 (0.6 mg/day), P3 (0.8 mg/day), and P4 (1.0 mg/day) of orally administered PET microplastics for 28 days. SGOT and SGPT levels were measured using spectrophotometry. Statistical analysis was performed using ANOVA followed by Tukey-HSD or Bonferroni post hoc tests (α=0.05). Results: The SGPT level in the control group (61.48 ± 7.50 U/L) significantly increased to 114.40 ± 7.71 U/L (P1), 132.22 ± 18.41 U/L (P2), 191.78 ± 16.42 U/L (P3), and 227.26 ± 12.23 U/L (P4). The SGOT level in the control group (78.64 ± 9.48 U/L) significantly increased to 188.46 ± 2.55 U/L (P1), 195.10 ± 5.75 U/L (P2), 238.90 ± 41.34 U/L (P3), and 310.74 ± 26.66 U/L (P4). Statistical analysis revealed significant differences among groups (p < 0.05). The higher the PET microplastic dose, the higher the SGOT and SGPT levels observed. Conclusions: ET microplastic exposure induces hepatotoxicity, as evidenced by dose-dependent increases in SGOT and SGPT levels.
Antibacterial Activity of Papaya (Carica papaya L.) Seed Ethanol Extract against Staphylococcus aureus Isolated from Diabetic Ulcers Putri Aprilia, Diah; Mufidah, Hartalina; Wijaya, Anas Fadli
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.30

Abstract

Background: Staphylococcus aureus is a bacterium that causes infections and is pathogenic to humans. Papaya (Carica papaya L.) seed extract contains various bioactive compounds, including tocopherols, terpenoids, flavonoids, alkaloids such as carpaine, and enzymes such as papain and lysozyme, which are known for their potential antimicrobial effects. Purpose: This study aimed to determine the antibacterial activity of papaya seed ethanol extract against S. aureus isolated from the foot ulcers of diabetic patients. Methods: An experimental quantitative study was conducted to evaluate the antibacterial activity of papaya seed ethanol extract at concentrations of 40%, 60%, and 80%. The extracts were prepared using the Ultrasonic-Assisted Extraction (UAE) method. Antibacterial activity was assessed by measuring the inhibition zones formed against S. aureus. Data were analyzed using a one-way ANOVA followed by a post hoc test to determine statistical significance. Results: The papaya seed extract exhibited concentration-dependent antibacterial activity against S. aureus. The mean inhibition zones were 0.00 mm at 40%, 15.07 mm at 60%, and 18.83 mm at 80%, whereas the positive control produced an inhibition zone of 46.36 mm. Conclusions: Papaya seed ethanol extract demonstrated antibacterial activity against S. aureus isolated from diabetic foot ulcers, with the strongest inhibition observed at the 80% concentration.
Effects of Various Mung Bean Decoction Doses on Hematological Profile in Anemic Wistar Rats Dwi Ayu Pratiwi, Nabila; Mufidah, Hartalina; Imroatul Muflihah, Ahdiah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.32

Abstract

Background: Anemia remains a significant global public health problem, with iron deficiency being the primary cause. Mung beans (Vigna radiata L.) are nutritionally rich plants containing iron and glycine, which are essential for hemoglobin synthesis and red blood cell formation Purpose: This study aimed to evaluate the effects of various doses of mung bean decoction on hematological profile improvement in anemic Wistar rats induced with sodium nitrite (NaNO₂). Methods: An experimental study was conducted from June to August 2024 involving 25 male Wistar rats divided into five groups: healthy control (aquadest), negative control (NaNO₂), and three treatment groups receiving NaNO₂ followed by mung bean decoction at doses of 0.2 ml, 0.4 ml, and 0.8 ml respectively. Blood samples were collected before and after treatment for hemoglobin level assessment. Data were analyzed using paired sample t-test and One-way ANOVA with post-hoc Tukey tests. Results: The One-way ANOVA test showed highly significant differences in hemoglobin level changes between groups (p < 0.0001). The 0.2 ml dose group exhibited the highest hemoglobin increase from 8.12 g/dl to 17.70 g/dl (p = 0.0002), followed by the 0.4 ml dose group with an increase from 7.32 g/dl to 16.08 g/dl (p < 0.0001), and the 0.8 ml dose group with an increase from 6.40 g/dl to 14.16 g/dl (p = 0.0005). Conclusions: Mung bean decoction effectively improved hemoglobin levels in anemic Wistar rats, with the 0.2 ml dose demonstrating the most pronounced therapeutic effect.
Identification of Candida albicans in the Urine of Pregnant and Non-Pregnant Women with UTI at X Hospital, Jember Pratiwi, Melia; Mufidah, Hartalina; Imroatul Muflihah, Ahdiah; Anggia Destiawan, Rian
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.33

Abstract

Background: Urinary tract infections (UTIs) are a common condition in older women, but adolescents can also experience them. This condition can lead to other conditions, such as cancer and urinary tract stones. Candida albicans infection is one cause of UTIs, which is highly prevalent in both pregnant and non-pregnant women. This is due, in part, to hormonal influences. Purpose: This study aimed to knowing the difference in the growth of Candida albicans fungus in the urine of pregnant and non-pregnant women suffering from urinary tract infections. Methods: The study design was cross-sectional with 30 urine samples from pregnant women and 30 urine samples from non-pregnant women with urinary tract infections. The study was conducted from March to May 2025. The sampling technique used was purposive sampling. The data obtained were processed using SPSS chi-square test. If the chi-square test requirements were not met, decision-making was based on the fisher's exact test value. Results: Positive results for Candida albicans in the urine of UTI in pregnant women were 13 samples and 3 negative samples, while in non-pregnant women, 9 positive samples and 1 negative sample were obtained. UTI did not have a significant effect on the growth of Candida albicans because the statistical analysis of the chi-square test obtained a p-value of 0.370, where the p-value is greater than 0.05. Conclusions: There was no difference in the growth of Candida albicans fungus in the urine of pregnant and non-pregnant women with UTI.
Effectiveness Of Starfruit (Averrhoa bilimbi L.) Extract Against Head Lice (Pediculus humanus capitis) Mortality Athira, Dini; Anggia Destiawan, Rian; Imroatul Muflihah, Ahdiah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.42

Abstract

Background: Head lice (Pediculus humanus capitis) are parasites that suck human blood. The disease caused by head lice is called Pediculosis which still occurs in densely populated areas such as Islamic boarding schools. The compounds contained in bilimbung wuluh fruit such as saponins and flavonoids are believed to help kill head lice. Purpose: This study aims to determine the effectiveness of star fruit extract (Averrhoa bilimbi L.) on the mortality of head lice (Pediculus humanus capitis). Methods: The method in this research is experimental with a Post Only Control Group research design. This study used 384 head lice samples with each group of 16 head lice samples sprayed with star fruit extract with concentrations of 10%, 20%, 30% and 40%. As well as using the gold standard anti-head lice drug Peditox and negative control in the form of sterile distilled water. Results: The results of this study indicate that star fruit extract (Averrhoa bilimbi L.) is effective in killing head lice (Pediculus humanus capitis) at concentrations of 20%, 30%, 40% within 30 minutes. However, at a concentration of 10% it is not effective in killing head lice. The Kruskal Wallis test showed that there was an effect of starfruit extract on head lice mortality with a significant value of 0.001 p-value ≤0.05. In the Maan-Whitney U follow-up test, it was found that there were differences in the test groups with a significant value of ≤0.05. However, in the 10% extract test group and the negative control group, there was no significant difference in values ≥0.05. Conclusions: Star fruit extract (Averrhoa bilimbi L.) is the most effective for killing head lice (Pediculus humanus capitis) within 30 minutes, namely a concentration of 40%.

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