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Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
Published by Universitas Syiah Kuala
ISSN : -     EISSN : -     DOI : -
Core Subject : Education, Engineering,
Engineering Other
Arjuna Subject : -
Articles 402 Documents
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Effect of Static extraction time on Extraction Efficiencies using On-line Supercritical Fluid Extraction-High Performance Liquid Chromatography for Lipoquinone Analysis in Activated Sludge Ni Luh G. R. Juliasih; Lee Chang Yuan; Yoichi Atsuta; Hirotsugu Kamahara; Hiroyuki Daimon
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 1 (2015): Engineering
Publisher : Syiah Kuala University

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Abstract

Analysis of microbial community is important for monitoring the performance of biological processes. One of the most simple, quantitative and high reproducible method for analysis of the microbial community is lipoquinone profile method. Lipoquinone is constituent of bacterial plasma membrane that is essential for electron transporter. Lipoquinone could be used as a biomarker to analyze the microbial community because in general one species or genus of bacteria has one dominant type of lipoquinone, thus any changes in the lipoquinone profile would reflect the changes in the microbial community. The method for lipoquinone determination in environmental sample is direct extraction using organic solvent and analysis using chromatography system. Since the method is tedious and uses a large amount of organic solvent, the on-line supercritical fluid extraction-high performance liquid chromatography (on-line SFE-HPLC) has been developed to simplify the method, and was successful determine lipoquinone compounds in activated sludges. The effect of static extraction time on extraction efficiencies of the lipoquinone was investigated in order to eliminate the water pump and methanol pump in the previous system.  The CO2 was used as an extraction solvent.  The 0.1 g of freeze dried activated sludge was placed into a 1 mL stainless steel extraction vessel and methanol was spiked into the sludge as a modifier. The SFE was connected to HPLC by using trapping column as an interface for collecting lipoquinone extracted from the sludge. The static extraction time was conducted by allowed the matrix to immersed in CO2 and methanol. When the static extraction time finished, the dynamic extraction time was carried out. The extracted and trapped lipoquinone then directly transferred to HPLC system for determination. In this study, the effect of static, dynamic extraction time and volume of spiked methanol were optimized using simplified on-line SFEHPLC for lipoquinone analysis. The best results in terms of extraction yield were obtained at 25 MPa, 45°C, 10 min static extraction time with 500 µL methanol spiked, and 25 min dynamic extraction time with 0.9 mL min-1 CO2 flow rate. It was concluded that the developed method could simplify the online SFE-HPLC system of lipoquinone determination which is useful for a rapid and routine analysis of microbial community in activated sludge.
In vitro antimalarial activity of Jaloh leaves extract on Plasmodium falciparum Nuzul Asmilia; Amalia Sutriana; Erdiansyah Rahmi; . Sugito
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Malaria still cause health problem in tropics including Indonesia, since its generate high mortality rate. Medication of Malaria has been performed long time ago without optimum result. One reason is the high rate of P.falciparum resistance on conventional antimalaria drug. This research aim to evaluate the activity of jaloh leaves extract (Willow leaves) on the inhibition of P.falciparum in vitro.This was a laboratory experimental research that using Indonesian isolates of cloroquin resistance P.falciparum obtained from NAMRU-2, Jakarta. P.falciparum isolate that has been synchronized were divided in to 3 groups of treatment : Jaloh leaves ethanolic extract, jaloh leaves ethyl acetate extract, and jaloh leaves n-hexane extract. Each group were then alloted into 5 dosage levels: 6,25ug/ml; 12,50ug/ml; 25,00ug/ml; 50,00ug/ml; and 100ug/ml. The observation of parasitemia degree were done 48 hours post-incubation in which the thick blood smear were colored with Giemsa and then examined using bioculer microscope. Data were analyzed using one way ANOVA and Newman-Keuls test. The result showed that the percentage of P.falciparum growth inhibition were sinificantly different (P0.05) among treatments wherein ethanol extract and ethyl acetate extract more inhibit P.falciparum growth than the hexane extract. The administration of different dosage also resulted in significance different (P0.05) of P.falciparum growth inhibition. This research concludes that jaloh leaves extract could inhibit P.falciparum growth.
In-dept review on adherence to therapeutic regimen and well-being among patients with type II diabetes mellitus Gloria S. E. Coyoca; Hanna C. P. Bejarasco; Veron G. T. Dalumbar; Eldon S. Mendoza
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Diabetes Mellitus or diabetes is an aggregation of metabolic diseases distinguished as a condition occurring when the body can’t utilize glucose from the foods eaten. In 2011, 366 million were diagnosed with diabetes mellitus with 4.6 million deaths and 50% among which are undiagnosed. According to the International Diabetes Federation in 2011, diabetes is expected to rise by about 552 million by the year 2030. Patient nonadherence can be a pervasive threat to health and well-being and carries an appreciable economic burden as well, yet, only few studies have been conducted to examine the relationship between adherence to therapeutic regimen and well-being among type 2 diabetic individuals The main intention of this study is to provide an in–depth review of the relationship between the adherence to therapeutic regimenand well–being among type 2 diabetic individuals in Iligan City. Descriptive Correlational design was utilized to describe the relationship between the variables under investigation. Modified standardized questionnaireswere given to 133 respondents who were chosen using a simple random sampling. Results showed that among the therapeutic regimen adherence investigated, most of the respondents have an average level of adherence to diet compared to medication, exercise, and stress reduction techniques in which their level of adherence scored good. For their well–being status, only mental scored good, while physical, emotional and spiritual scored average. Furthermore, findings showed a significant inverse relationship between age ofrespondents and their level of adherence to diet, the respondents’ work status and their level of adherence to diet and stress reduction techniques. Positive significant relationship was found between the respondents’ educational attainment and their level of adherence to therapeutic regimen, economic status and medication adherence, and educational attainment and mental well–being. Therefore, therapeutic regimen adherence was found to have a positive impact on the well–being of the respondents. Collaborative team consisting ofendocrinologists, nurses, nutritionists, dieticians, exercise physiologists, and spiritual leaders is highly recommended for the intensive management of diabetes patients, thus, improving adherence and well–being
Isolation and characterization of antimicrobial constituents from a terrestrial Streptomyces sp.: A preliminary result Muhammad Bahi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Five phenolic derivatives, namely 2-methoxybenzene-1,3-diol (1), 2,3-dihydroxy-benzamide (2), benadrostin (4), 2,3-dihydroxy-benzoicacid (5) and tyrosol (6) were isolated from the terrestrial Streptomyces sp. Ank75. Three of them are new natural products. A further new antifungal is an azoxy derivative 3. All structures were elucidated based on spectroscopic and MS data and will be discussed here.
The effect of excretory/secretory product released by L3 of A. galli on villous compact in intestine of laying hens Ummu Balqis; . Darmawi; Risa Tiuria; Bambang P. Priosoeryanto
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The nematode, Ascaridia galli, caused pathological changes in the gut, while the larval development takes place in the intestine epithelium. The aim of this research was to examine the effect of excretory/secretory product released by L3 of A. galli to prevent intestine destructions based on villous compact in duodenum, jejunum, and ileum of laying hens. Excretory/secretory was prepared from metabolic of L3 released in culture médium in flasks containingrosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Excretory/secretory product was concentrated with vivaspin 30.000 MWCO. The chickens vaccinated with 80 g protein excretory/secretory in crudemixed with Fruend Adjuvant Complete and repeated three times with dose of each 60 g mixed with Freund Adjuvant Incomplete with an interval of one week intra muscularly. The chickens were challenged with 1000 infective larvae A. galli one week later. After two weeks the chickens were operated. Villous compact in duodenum, jejunum, and ileum of laying hens were analyzed. The result showed that immunization was able to sustained significantly intestinevillus compact, particularly in duodenum. This research concluded that the excretory/secretory product could protect villus in duodenum against parasitic diseases caused by A. galli.
Effect of Organic Matters And Water Stress On Performance of Rice in Vegetative Phase Cut Nur Ichsan; Erida Nurahmi; Oktrya Rochmad; Bakhtiar Bakhtiar; Efendi Efendi; Sabaruddin Sabaruddin
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Drought become a major limiting factor in world cereal production. Various methods are used to solve this problem such as irrigation management by managing the soil moisture effectively for the  plants  to  grow  normally.  Another  way  to  improve  the  water  holding  capacity  with  the addition  of  organic  matter  in  soil.  Another  way  by  planting  drought  tolerant  rice  varieties. Drought-tolerant  rice  varieties  like  Inpago  8  with  various  doses  of  organic  matter  and  water stress at vegetative phase treatment done  to analyze  the  change of rice plant performance at the  vegetative  phase  of  the  rice  crop  due  to  drought  and  organic  matter  dose.    At  very  low water  content,  which  happened  repeatedly  during  the  vegetative  phase  causes  the  change  in root growth, plant height, length and width of rice leaf significantly
Early detection of rotor-bar faults of three-phase induction motor using motor current signature analysis method . Syafrudin; Arnawan Hasibuan
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 2 (2011): Engineering
Publisher : Syiah Kuala University

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Abstract

Three-phase induction motors play a pivotal role in industry and there is a strong demand for their reliable and safe operation. They are generally reliable but eventually do wear out. Faults and failures of induction motor can lead to excessive downtimes and generate large losses in terms of maintenance and lost revenues, and this motivates the examination of on-line condition early detecting.  On-line condition early detecting involves taking measurements on a motor while it is operating in order to detect faults with the aim of reducing both unexpected failures and maintenance costs. Motor Current Signature Analysis (MCSA) method is a way to detection of condition monitoring technique used to early detection problems in rotor bar fault of three- phase induction motors. In this paper presented the experimental results of frequency spectrum Fast Fourier Transform (FFT) stator current on-line censorship a three-phase induction motor using LabVIEW algorithm for detecting of rotor conductors faults. The stator current FFT analyzed using Motor Current Signature Analysis (MCSA) method.
Didekilketon compounds from the leaves of Artocarpus camansi Blanco Rosnani Nasution; . Marianne
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Research on plant leaves Artocarpus camansi (kulu), aims to determine the chemical compounds contained in the hexane extract of the plant leaves. This study begins by isolating the hexane extract, from the leaves of plants A. camansi. Subsequently the extracts were characterized by GC-MS, to determine the fragmentation pattern softhe compunds contained in leaves of A. camansi. Furthermore the hexane extract further fractionated to obtain pure isolates. Pure isolate of the compound as white solid with a melting point of176-1780C. Characterized to the pure compound with1H-NMR, 13C-NMR, DEPT and reinforced with HSQC, and HMBC, expressed asdidekilketon (C21H42O).
Ordinal Measure of Discrete Cosine Transform Blocks for Iris Identification Fitri Arnia; Fery Irianda; Siti Aisyah; Khairul Munadi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 2 (2012): Engineering
Publisher : Syiah Kuala University

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Abstract

Currently, a common method for identifying a person is by means of an identitycard (ID) or combination of an ID and password. The approaches are not very reliable, since the ID can be stolen and password can be forgotten. A more reliable identification system is required. In the last decades, identification systems based on biometrics have been gaining attention, since they are more reliable. Biometrics-based devices identify people based on their physical or psychological characteristics, such as palmprints, fingerprints, gait and iris. Unlike fingerprints or palmprints, irides features distribute randomly, and the features were unique; the features between right and left eyes aredifferent, as well as between twins. Therefore, in addition to reliability, the use of irides can enhance identification accuracy. Purpose of the paper was to improve identification rate of an iris identification method, using ordinal measure of Discrete Cosine Transform (DCT) coefficient. The input iris image was tiled into blocks of 8x8 pixels, then the DCT was applied to each blocks. The AC coefficients of each block were sorted from the smallest to the largest values, in which the sorted values were referred to as ordinal measures.Identification was accomplished by measuring a distance between the ordinal measure of the input images with the ones of the existing images in the database using Minkwoski distance metric. Proposed method increased the averaged identification rate as compared to the previous method by nearly twice from 33% to 61.4%.
Effect of serving factors of milk powder in sachet packaging on total microbial Dwi A. Ratih; . Nurliana
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The aim of this research was to study the microbial quality of milk powder based on the serving factors during milk  powder serving  process. The method used in this research was Total Plate Count (TPC). Nine sachets (400 g/sachet) of milk powder was used as many as This experiments used Split Plot Design, comprised three factors, storage factor (P1, P2 and P3), time of serving factor (morning and evening), and shelf life factor (day of 1st-7th). Each treatment consisted of three sachets of milk powder. Storage factors comprised P1 (sachets packaging contained of milk powder were placed into the glass bottle container), P2 (sachets packaging contained of milk powder were placed into glass botlle with spoon inside, and P3 (sachets packaging contained of milk powder with spoon inside were not placed into the glass bottle container). The results of this research showed that storage factors and time of serving did not sifnificantly (P0,05) affect on the microbial count of milk powder, but shelf life factor significanly (P0,01) affected on microbial count of milk powder at day of 1th and 7th. Milk powder in sachet packs (400 grams) has  been opened, with or without spoon in the sachet and more than five days of shelf life can increase the total number of microbes, which was exceed TPC decision at 5x104 CFU/grams of milk powder.

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