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Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
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Articles 402 Documents
The Effect of propolis to blood glucose and total cholesterol of prediabetes patients Andi N. Usman; Andi Z. Abdullah; Buraerah A. Hakim; Nurhasni Hasan; Andi Ariyandi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Prediabetes is considered as the initial phase of macrovaskular disease associated with increase of blood glucose and cholesterol level. The effect of propolis to treat fasting blood glucose, glucose tolerance, total cholesterol was examined. Experimental Research with Randomized Clinical Trial (RCT) design was utilized in the study. Intervention given was propolis at dose 50 mg/kg bwt and health education administered for 20 days. The quality of propolis was 20%extract and quercetin content 25.29 mg/L tested by Biofarmaka Laboratory Test of Hasanuddin University, health education used counseling strategy. Thesamples were 64 prediabetic patients consist of 32 patients treated with propolis and 32 treated with health education. The result of Wilcoxon with significant level of 0.05 has proved significantly decreased fasting blood glucose, glucose tolerance and total cholesterol respectively 14.28 (p=0.000), 23.16 (p=0.000) and 16.3 (p=0.000) The result of group given propolis as significant as health education group respectively fasting blood glucose 14.9 (p=0.001), glucose tolerance 13.98 (p=0.000) and total cholesterol 9.76 (p=0.021). Giving propolis and health education are effective for the change of fasting blood glucose,glucose tolerance and total cholesterol. Propolis is potential to use as a pharmacology therapy for prediabetes.
Predicting of Tsunami Inundation Area based on Propagation and Runup Numerical Model in Pacitan City Agus Suharyanto; Alwafi Pujiraharjo; Adipandang Yudono; Keisuke Murakami; Chikashi Deguchi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 2 (2012): Engineering
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Abstract

Pacitan city represents one of the regions residing in South East Java ProvinceIndonesia about which on direct with Indian Ocean. In this area there is a meeting of plate of Indo-Australia with plate of Eurasia which is if colliding head-on potency can generate tsunami. Seeing this existing condition, it is needed a study to know how susceptible level of South East Java Province regional to get disaster of tsunami. Based on the tsunami was occurred in Aceh Indonesia on December 26, 2004 was killed more than 200.000 peoples, the tsunami disaster management become very important to study. One of the studies ishow to predict the tsunami runup along shoreline of southern beach of East Java Province Indonesia. According to the geological data, area of east Java Ocean has high potential of earthquake. The history shows that there are many time tsunami occurred in East Java. Based on the initial study, one of the areas predicted as dangerous area for the tsunami is Pacitan city. To minimize the effect of tsunami hazard, prediction of inundation area base on the numerical model simulation of tsunami runup was done for Pacitan city. In this research predicting of inundation area caused by tsunami was studied. GIS was used astool to predict the inundation area. From this research it can be shown that numerical model of tsunami can be used to predict the runup. Base on runup elevation, inundation area can be predicted well by using GIS
Analysis on the quality of test items on disaster management block Rosaria Indah; . Mulyadi; Reza Maulana
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 3 (2013): Addendum
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Abstract

Written exam is the format most often used to assess the extent of student learning achievement. The advantages of written exam are more easily prepared and relatively more economical when compared with clinical examination test. Block’s written exam, especially on disaster management block is considered important because it will illustrate the students' competence at the end of this special block. This study is a qualitative research, aimed to evaluate the quality of test on disaster management block.  The quality of the test were evaluated using qualitative method by deep interviewing 5 criteria of students.  The result was analized using Miles and Huberman method and the themes emerged were: 1) The test item in disaster management block were fairly easy 2) Test items were in accordance with the competence of undergraduate, 3) The test item were congruent with the content material taught in this block.  However, there were some weaknesses, including: 1) There were repeated items, 2)  There were format of question that complicates matters without a clear purpose, 3) There were ‘easy to predict’ item questions, 4) Lack of variation of format.  These problems might cause low discriminant items.  Recommendation including continues improvement of item developers’ capability by integrate it within curriculum for faculty development programs
IL-12 PE, CD 69 PERCP, CD3 FITC, AND CD4 APC OPTIMIZATION WITH ACTIVATION OF ISOLATED AGENT HEAT-KILLED SONICATED MYCOBACTERIUM TUBERCULOSIS BEIJING STRAIN RINI SUNDARI; IDA PARWATI; JOHANES C. MOSE; BUDI SETIABUDIAWAN
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
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Abstract

Infection caused by Mycobaterium tuberculosis exists in form of intracellular infection, which leads to lymphocyte activation. CD69 is the first lymphocyte activation marker expressed in Th1 lymphocyte, which follows by IL-12 release. Flow cytometry analysis can identify the subpopulations of lymphocytes and  intracellular cytokines such as IL-12, yet precise preparation needs to be done. This research aims to conduct optimization with four color lyse/wash flow cytometry assay system FastImmune™ FACSCalibur examination, with monoclonal antibody IL-12, CD69, CD3, and CD4 in succession uses fluorochrome PE, PerCP, FITC, and APC.To activate the lymphocytes from heparinized whole blood, we used activation agent which derives from isolated heat-killed sonicated Mycobacterium tuberculosis Beijing strain. Optimal concentration from the according activation agents is 40 mL. To determine the compensation, BDTM CompBead and blank-cell unstainning are used, but the maximum result showed by blank-cell unstainning.Each monoclonal antibody dosage of IL-12PE, CD69 PerCP, and CD3 FITC is 40 mL, while CD4 APC 5 mL. Total event lymphocyte is determined minimally by 10,000 events. With 18,510 total events and Th gated events quantity are 4,692, the result obtained is IL12-PE has 7.4% gated (347 events); CD69+ perCP/CD3+ FITC 18.2% (850 events); and CD69+ perCP/CD4+ APC 3.9%.
Enzyme activity of Ascaridia galli Llarvae was inhibited by Phenil Methanyl Methane Sulfonyl Fluoride Ummu Balqis; . Darmawi; Muhammad Hambal
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
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Abstract

Enzyme catalyse a broad spectrum of important biological reactions, including protein metabolism and immune reactions. A study was carried out to partial characterize enzyme from exretory/secretory of A. galli L3 stage based on influence of inhibitor. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. Excretory/secretory was prepared from metabolic product of L3 released in culture medium. The enzyme activity was assayed against casein 2%. The amount of concentration was determined from the absorbance at 595 nm. Inhibitor sensitivity on enzyme activity was studied in phenil methanyl methane sulfonyl fluoride (PMSF) 0,5 and 1 mM.    The result showed that enzyme activity of A. galli larvae was inhibited by PMSF 0.5 and 1 mM, which enzyme activity remainded 1,8 and 0%, respectively. The results indicate that the excretory/secretory secreted by L3  A. galli contained serine protease
Synthesis and characteristic bioadditive of rubber seed oil as low pour point biodiesel CPO . Misdawati; Muslih Nasution; . Syafiruddin
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
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Abstract

The bigest point from CPO biodiesel is the high pour point, which is if used at low temperature will cause blockage the filter and fuel lines that make some problem in machine. To solve this problem was using pour point lowering additive, the widely used additive now was chemical commercial additive,which is have highest prices. Methyl ester that made form rubber seed oil have high free fatty acid needed two step reaction, esterification reaction to lowering the free fatty acid to £ 2% and transesterification reaction. The reaction variable was temperature, rubber seed oil/methanol ratio mol and concentrations ofKOH catalyst. Response Surface Methodology was used at transesterificstion reaction, first to know interaction variables effect in reaction, and seconded to get the optimum point. Optimum yield was obtained at 1/6 oil/methanol ratio mol, 1% concentration KOH catalyst and temperature 60°C with time reaction was 90 minutes. Reaction at the optimum condition obtains 99.61% conversion methyl ester (bioadditive) rubber seed oil.
Systemic lupus erythematosus and worsening severe preeclampsia: Does it have correlation? Eva Febia; Cut M. Yeni
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
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Abstract

Acute exacerbation of systemic lupus erythematosus in pregnancy complicated by severe preeclampsia was difficult to diagnose and treat. This is the case of Mrs Y, 26 years old in 24 weeks gestational age with severe preeclampsia with active exacerbation of systemic lupus erythematosus. She had suffered from systemic lupus eryhtematosus for three years before she got pregnant. When she came the blood pressure was 180/110 mmHg, protein urine +3. There was complaint of stiffness on muscle joints, facial flares, general weakness, and photosensitivity. She was hospitalized for seven days before she was discharged. Her blood pressure was controlled and lupus symptoms were controlled by antihypertensive agents and antiinflamation agent metil prednisolone. A week later she came back to our hospital. Her blood pressure was 190/110 mmHg despite having antihypertensive. She also complaint of joints ache, general weakness, facial flares, and no fetal movement. On ultrasound examination there was intra uterine fetal death. From this case, we would like to assess the patophysiology of systemic lupus erythematosus and worsening symptoms of severe preeclampsia which cause the mortality and morbidity of mother and baby
The effect of temperature on cr (vi) removal by using guava leaves as a biosorbent . Mariana; Farid Mulana
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 2 (2013): Engineering
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Abstract

Preliminary research on Cr(IV) removal in a liquid waste using guava leaves (Psidium Guajava) as a bio-sorbent has been done. The experiments were conducted in a stirred batch reactor. The effect of temperature on efficiency and absorption capacity has been studied. Both the efficiency and absorptioncapacity are directly proportional to the temperature under the current experimental conditions. The results showed that optimum absorption capacity and absorption efficiency of Cr(VI) use of guava leaves obtained at stirring time 30 minutes.
The use of herbal medicine in children Suryawati Suryawati; Hijra Novia Suardi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
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Abstract

The herbal medicine has been widely used in children for the treatment of several symptoms and the prevention of diseases before accessing the hospital for professionals help. There are 3 kinds of marketed herbal medicine including empirical based herbal medicine (jamu), standardized herbal medicine (obat herbal terstandar) and clininically tested herbal medicine (fitofarmaka). This study aimed to investigate the utilization of the marketed herbal medicine along with non marketed ones which was known as the Indonesian original herbal medicine (Obat Asli Indonesia, OAI) in children. The cross sectional study was conducted by interviewing 91 parents whose children were hospitalized in RSUD Meuraxa, Banda Aceh. The interview was conducted using a structured questionnaire from 4 to 29 September 2014. The result showed that the Indonesian original herbal medicine was used most frequently followed by herbal (jamu), standardized herbal and clininically tested herbal medicine. The utilization of herbal medicine was associated with the knowledge of parents and did not correlate to their economic level. All of the parents did not know the side effects of herbal medicine to their children’s body and  few of them knew  the indication of herbal  remedies. Overall, it was  concluded  that eventhough the knowledge of parents about the safety and activity of herbal medicine was not sufficient the use of herbal medicine in children was common
Antifungal properties of cassava starch edible film incorporated with lemongrass essential oil YULIA ANNISA; ISMAIL SULAIMAN; NOVI SAFRIANI; M. DANI SUPARDAN
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 1 (2014): Engineering
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Abstract

The aim of this study was to evaluate the antifungal properties of cassava edible film incorporated with lemongrass essential oil against two strains of fungal, Trichoderma sp and Penicillium sp. Edible films were prepared from a mixture of cassava starch and glycerol. Lemongrass oil (0.5%, 1%, 2%, 3% and 4% w/w) was added to edible films as natural antifungal agent. The agar disc diffusion method was used to determine the antifungal activity of cassava starch edible film. Antifungal behaviour was determined by the mold absences upon the film and by the inhibition zone formed. The experimental results showed that Trichoderma sp and Penicillium sp was not appear above the film until the fourth day. However, cassava starch edible films containing lemongrass oil did form any inhibition zone toward both of fungal