Garuda - Garba Rujukan Digital

VIABILITAS DAN KERIAP Bacillus sp. BK17 DAN Enterobacter sp. BK15 PADA SUMBER KARBON DAN NITROGEN YANG BERBEDA Sirma Novita Nasrah; Dwi Suryanto; It Jamilah
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.855 KB)

To propagate bacterial cell for biocontrol purpose, suitable nutrient have to be determined in which carbon and nitrogen source was often as limited factor of bacterial growth. Proper storage for biocontrol agent such as bacterial cell should also be considered in order to keep the cell viable when used. The aim of this study is to find out suitable carbon and nitrogen sources for viability and swarming of chitinolitic bacterial Bacillus sp. BK17 and Enterobacter sp. BK15. The highest population of bacterial growth (3.7x108 cfu/ml) was found in molases-sodium nitrate (MS) medium and the lowest population was found in crab shell-sodium nitrate (CS) growth (2.4x108 cfu/ml) after 25 days of incubation. The swarming activity of the isolates were varied to some extent with the highest was 51 mm in 2% agar molases-urea after 5 days of incubation. Molases-sodium nitrate (MS) medium is suitable carbon and nitrogen source for the viability of Bacillus sp. and Enterobacter sp. Meanwhile, agar molases-urea medium with 2% agar is suitable medium for swarming ability for both bacteria. Keywords : Bacillus, C and N-source, Enterobacter, swarming, viability

PENGHAMBATAN PERTUMBUHAN Aspergillus flavus DAN Fusarium moniliforme OLEH EKSTRAK SALAM (Eugenia polyantha) DAN KUNYIT (Curcuma domestica) Ira Wulan Dani; Kiki Nurtjahja; Cut Fatimah Zuhra
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.582 KB)

Growth inhibition of Aspergillus flavus and Fusarium moniliforme by leaf extract of salam (Eugenia polyantha) and turmeric (Curcuma domestica) was studied. Kirby-Bauer disc diffusion method was used in this experiment. The plant extracts used were 0, 5, 10, 15, 30, 40, 50, 60 and 70% and dimethyl sulfoxide (DMSO) as a solvent. The result showed that the E. polyantha and C. domestica have a different activity in inhibiting the growth of A. flavus. The leaf extract of turmeric showed lower activity inhibiting A. flavus and F. moniliforme than that of E.polyantha. In 5% E. polyantha inhibits the growth both of the fungus. While C. domestica showed activity inhibiting A. flavus in 5 % and F. moniliforme in 40 %. Keywords: A. flavus, F. moniliforme, extract salam, turmeric

PENGHAMBATAN PERTUMBUHAN Aspergillus flavus DAN Fusarium moniliforme OLEH EKSTRAK SERUNI (Wedelia biflora) DAN KEMBANG BULAN (Tithonia diversifolia) Agnes Dame Sinta Uli; Kiki Nurtjahja; Cut Fatimah Zuhra
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.214 KB)

The growth inhibition of Aspergillus flavus and Fusarium moniliforme by extract of seruni (Wedelia biflora) and Kembang bulan (Tithonia diversifolia) has studied. Kirby-Bauer disc diffusion were used in this experiment. The leaf methanolic extracts prepared were 0, 5, 10, 15, 30, 40, 50, 60 and 70% with dimethyl sulfoxide as the solvent. The results showed that extract of seruni and kembang bulan has a different activity in inhibiting the growth of both fungus. Seruni extract showed lower activity in inhibiting A. flavus and F. moniliforme than kembang bulan. In 40% seruni inhibits the growth both of the fungus. While kembang bulan showed activity in inhibiting A. flavus and F. moniliforme in 5%. Kata kunci: A. flavus, F. moniliforme, T. diversifolia and W. biflora exctract

KELAINAN PERKEMBANGAN KRANIOFACIAL FETUS MENCIT (Mus musculus L.) Strain DDW SETELAH PEMBERIAN EKSTRAK N-HEKSAN BUAH ANDALIMAN (Zanthoxylum acanthopodium DC.) Mai Sarah; Emita Sabri; Salomo Hutahaean
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.894 KB)

The purpose of this study is to investigate the effect of n-hexane extract of andaliman (Zanthoxylum acanthopodium DC.) fruit on craniofacial development of DDW Mice (Mus musculus L.). Experiment was carried out using Completely Randomized Design (CRD) with 5 treatments and 6 replicates. Treatments consist of a blank control, vehicle control (1% CMC) and 3 concentrations of andaliman extract (2%, 4% and 6%). All treatments were administered by oral gavage (0.1 ml/10 g bw) once daily from day 0 to day 10 of gestation. N-hexane extract of andaliman fruit induced developmental abnormalities in craniofacial structures i.e. increased the incidence of microphthalmia (P <0.05), and hydrocephalus (P <0.05). In conclusion, we suggested that andaliman fruit may have fetotoxic effects. Keyword: andaliman (Zanthoxylum acanthopodium DC.), micropthalmia, hydrocephalus

UJI POTENSI BAKTERI KITINOLITIK DALAM MENGHAMBAT PERTUMBUHAN Rhizoctonia solani PENYEBAB REBAH KECAMBAH PADA KENTANG VARIETAS GRANOLA Dewi Novina; Dwi Suryanto; Elimasni D
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.162 KB)

A study on assay the potential of chitinolytic bacterial isolates to inhibit the growth of Rhizoctonia solani causal agent of damping-off on potato was carried out in Laboratory of Observation Pest and Disease, Medan Johor, UPT. Protection of Crops and Holticulture 1 and Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Science of Universitas Sumatera Utara, Medan. Two out of six isolates of chitinolytic bacteria showed capabilities to inhibit of R. solani growth. The most effective isolates to inhibit the growth of R. solani were Enterobacter sp. BK15 and Bacillus sp. BK13, that were able with inhibition zone of 2.05 and 2.35 cm, respectively. These two isolates also showed to be able to reduce fungal infection by 31.25% and 37.5%, respectively. Keywords: Bacillus sp. BK13, damping-off, Enterobacter sp. BK15, R. solani, potato

PEMANFAATAN BAKTERI KITINOLITIK DALAM MENGHAMBAT PERTUMBUHAN Curvularia sp. PENYEBAB PENYAKIT BERCAK DAUN PADA TANAMAN MENTIMUN Andini - Hanif; Dwi Suryanto; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.48 KB)

A study on about the utilization of chitinolytic bacterial isolates to inhibit growth of Curvularia sp. causal agent of leaf spot disease of cucumber was done in Laboratory of Pest and Disease, Medan Johor, UPT-Balai Protection Plant and Horticulture I and Laboratory Microbiology Departement of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan. Six chitinolytic isolates were tested in vitro to inhibit growth of Curvularia sp.. The result showed that Bacillus sp. BK13 inhibited more with inhibition zone of 2,75 cm and Enterobacter sp. BK15 with inhibition zone of 2,55 cm, whereas Enterobacter sp. PB17 showed the lowest inhibition zone of 1,3 cm. Soaking seed treatment in chitinolytic bacterial suspension reduced the percentage of leaf spot. Enterobacter sp. BK15 showed more ability (50%) to inhibit leaf spot attack. Keywords: Bacillus sp. BK13, cucumber, Curvularia sp., Enterobacter sp. BK15, leaf spot

GAMBARAN HISTOLOGIS HEPAR MENCIT (Mus musculus L.) STRAIN DDW SETELAH PEMBERIAN EKSTRAK N-HEKSAN BUAH ANDALIMAN (Zanthoxylum acanthopodium DC.) SELAMA MASA PRA IMPLANTASI DAN PASCA IMPLANTASI Eka Prasetiawan; Emita Sabri; Syafruddin Ilyas
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.17 KB)

Liver histological features of DDW mice (Mus musculus L.) treated with n-hexane extract of Andaliman (Zanthoxylum acanthopodium DC.) fruit during pre- and post-implantation has been studied in Laboratory of Animal Structure, Department of Biology, Faculty of Mathematics and Natural Science, University of Sumatera Utara, Medan. Experiment used completely randomized design (CRD) consisted of 2 periods of treatment: pre-implantation (day 0-3; group A) and post-implantation (day 6-14; group B). For each group, mice were treated daily by oral gavage with 0, 1, 2, 4, or 6 % of andaliman extract in 0.3 ml of 1% CMC. The result showed, liver in treatment groups was pale and showed mottled surface texture. Hepatocyte damage in post-implantation group was higher than in pre implantation group (p> 0.05). We concluded that n-hexane extract of andaliman fruit appears to negatively affect liver histology of mice. Keywords: liver histology, andaliman (Zanthoxylum acanthopodium DC.), post implantation, pre implantation

PENGENDALIAN SEL BIOFILM BAKTERI PATOGEN OPORTUNISTIK DENGAN PANAS DAN KLORIN Yusnita Wahyuni Silitonga; It Jamilah; Dwi Suryanto
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.282 KB)

Opportunistic pathogenic bacteria are bacteria that are naturally not present in an environment that, but it dues to contamination of the environment by human waste. From previous research it was found E. coli, Staphylococcus sp. and Salmonella were opportunistic pathogens of the shrimp aquaculture (Percut, Pantai Labu, Pantai Cermin). The aims of this study is to know the ability of these bacteria to form biofilms as well as its control using chlorine and heat. In order to test the ability of the bacteria to form biofilms, the stainless steel have been soaked in SWC media for 1, 3, 6 days. E. coli, Salmonella, and Staphylococcus sp. may form biofilms on incubation of the 1st day but the highest growth was on the 6th day of incubation by E. coli with a 6,35 x 104 CFU/SS, whereas of the lowest biofilm number was found in Salmonella with a 0,28 x 104 CFU/SS. The number of biofilm cells grow in line with a length of incubation. In this research, the most effective concentration of chlorine to kill biofilm cell was 225 ppm for 2 minutes and heat was 100 0C for 5 minutes. The higher the concentration of chlorine and the temperature given more effective to kill the bacteria. Keyword: biofilm, chlorine, heat, opportunistic, stainless steel

PENGHAMBATAN SERANGAN Sclerotium rolfsii PENYEBAB REBAH KECAMBAH PADA KEDELAI DENGAN BAKTERI KITINOLITIK Novi Malinda; Dwi Suryanto; Kiki Nurtjahja
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (504.203 KB)

The inhibition of Sclerotium rolfsii as causal agent of soybean damping off by using chitinolytic bacteria were studied in Laboratory of Observation Pest and Disease UPT-BPTPH 1 and Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan. The aim of this research was to investigate the ability of chitinolytic bacteria to inhibit the growth of S. rolfsii. Six isolates of chitinolytic bacteria were used in this study. The inhibition zone between colony of the bacteria and the fungus was measured. The result showed that the bacterial isolates has different ability in inhibiting the growth of S. rolfsii. Enterobacter sp. BK15 and Bacillus sp. BK13 were the most effective in inhibiting the growth of S. rolfsii with inhibition zone of 3.70 cm and 3.75 cm, respectively. The less effective was shown by Enterobacter sp. PB17 with inhibition zone of 0.10 cm, while Enterobacter cloacae LK08 have no ability to inhibit S. rolfsii. The seeds soaked in bacterial suspension by Bacillus sp. BK13 dan Enterobacter sp. BK15 were able to reduce damping off by 44.4% and 50%, respectively. Keywords: Bacillus sp., damping off, Enterobacter sp., Sclerotium rolfsii, soybean

POTENSI BAKTERI KITINOLITIK DALAM PENGENDALIAN Aspergillus niger PENYEBAB PENYAKIT BUSUK PANGKAL AKAR PADA TANAMAN KACANG TANAH Arifda Ayu; Dwi Suryanto; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.174 KB)

A study of chitinolytic bacteria ability to control Aspergillus niger, a causal agent of basal root rot of peanut seedlings was conducted in Pest and Disease Laboratory, Medan Johor and Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan. The purpose of study is to evaluate the ability of Bacillus sp. BK13, Enterobacter sp. BK15, Bacillus sp. BK17, Enterobacter cloacae LK08, Bacillus sp. KR05, and Enterobacter sp. PB17 to inhibit the growth of A. niger on the peanut seedling. Antagonistic test showed that the most effective bacteria in inhibiting the growth of A. niger was BK15 isolate with inhibition zone of 2,88 cm and BK13 isolate with inhibition zone of 2,69 cm, whereas the least effective bacteria was BK17, with inhibition zone of 2,30 cm. Chitinolytic bacterial isolates used to cover peanut seed through soaking enabled to reduce seed basal root rot. BK15 isolate showed potential to reduce the basal root rot by 58,82% while the lowest inhibition was BK13 by 47,06%. Keywords: Aspergillus niger, Bacillus sp., chitinolytic bacteria, Enterobacter sp., peanut

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota medan,

Sumatera utara

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kota medan, Sumatera utara INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota medan,

Sumatera utara

INDONESIA