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Saintia Biologi
Published by Universitas Sumatera Utara
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Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
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Articles 25 Documents
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GAMBARAN HISTOLOGIS PULMO MENCIT JANTAN (Mus musculus L.) SETELAH DIPAPARI ASAP ROKOK ELEKTRIK Nanin Triana; Syafruddin Ilyas; Salomo Hutahaean
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

The effects of electric cigarette exposure on lung tissue of mice were studied. The experiments using completely randomized design (CRD) with 3 treatments, i.e.  treatment of electric cigarette with strawberry flavour, tobacco flavour, and control treatment that was not exposed by cigarette smoke. Eight male mice were used for each group. Exposure treatment was done every day for 2 consecutive weeks with dosage 20 times suction using syringe of 60 mL. After receiving treatment for 2 weeks, the mice were killed by cervical dislocation. The lungs were isolated and prepared histologically using paraffin method and then stained with by haematoxylin and eosin  (HE). The results showed that, smoke treatment mice lose weight significantly (p< 0.05). In gross anatomy, electric cigarrete smoke affect lung feature, but histological observation on  membrane damage, damage to the lumen and the relationship between alveoli the smoke effect were statistically unsignificant (p>0.05).. In summary short period exposure to electric  cigarette smoke  tends affect lung damage in anatomy, but statistically has no effect of the damage to the lung tissue.   Keywords: alveolar lumen, alveolar membrane, electric cigarettes
ISOLASI DAN UJI POTENSI BAKTERI TANAH PERTANIAN BERASTAGI SUMATERA UTARA DALAM MENDEGRADASI FUNGISIDA ANTRACOL BERBAHAN AKTIF PROPINEB Diah Sri Utami; Nunuk Priyani; Erman Munir
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

Isolation and potential Berastagi agricultural soil bacteria, North Sumatra in degrading propineb-based antracol fungicide has been conducted. The isolated bacteria were grown on Bushnell Hass Broth (BHB) containing 2% of propineb-based antracol fungicide with propineb as the active compound. The cultures have been grown on shaking incubator at 150 rpm for 21 days. The media BHB containing 2% of propineb-based antracol fungicide without bacteria was used as a control. The parameters observed were the growth bacterial, biosurfactant activity, biosurfactant concentration and the residues of propineb which were observed on day 0th, 7th, 14th and 21th. A total of sixteen bacterial isolates were isolated using selective media Bushnel Hass Agar (BHA) containing 2% propineb-based antracol fungicide​​. Two bacterial isolates which were CBA 02 and JBA 04 were selected for further test to determine their ability to degrade propineb. JBA 04 showed much higher ability in reducing propineb concentration up to 60.86%, while CBA 02 was only 5.59% than that of control.   Keywords: biodegradation, bioremediation, biosurfactant, fungicides, propineb
PENGARUH PEMBERIAN VITAMIN C DAN E TERHADAP GAMBARAN HISTOLOGIS HEPAR MENCIT (Mus musculus L.) YANG DIPAJANKAN MONOSODIUM GLUTAMAT (MSG) Ahri Maulida; Syafruddin Ilyas; Salomo Hutahaean
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

This study aimed to determine the effects of vitamin C and E on liver microstructure of mouse exposed to monosodium glutamate (MSG) for 30 days. The experiment used completely randomized design which consists of 6 treatments and  5 replications. Mice were treated daily by oral gavage with 4 mg MSG (P1),  4 mg MSG + 0.26 mg  vitamin C (P2),  4 mg MSG + 0.026 vitamin E (P3), or  4 mg  MSG + 0.26 mg  vitamin C + 0.026 mg vitamin E (P4). All dosages were based on mg/g body weight. Two control groups K- and K+ were treated with water and castor oil respectively. The mice liver tissue slide was prepared by the paraffin method and stained with haematoxylin-eosin (HE). The results showed that MSG caused cell degeneration and necrosis in liver tissue. Vitamin C and E was  able to recover  liver cell damage induced by MSG.  The highest result was found in P3 and combination treatment (P4) where the recovery effect reaches 64% and 80% respectively. Keywords : Liver, Monosodium glutamate (MSG), Vitamin C, Vitamin E.
KOMPOSISI DAN STRUKTUR RERUMPUTAN DI KAWASAN DANAU TOBA DESA TOGU DOMU NAULI KECAMATAN DOLOK PARDAMEAN KABUPATEN SIMALUNGUN SUMATERA UTARA Pinta Omas Pasaribu; M Zaidun Sofyan; Nursahara Pasaribu
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

The composition and structure of grasses in Toba Lake Area Togu Domu Nauli Village, Dolok Pardamean Subdistrict, Simalungun Regency, North Sumatra has been studied from March to June 2012. The study site is determined by purposive sampling method and it is divided three locations based on altitude with 20 plots of  1 x 1 m size for each area. There are 21 species of grasses that belong to two families found at three study sites. The first area was dominated by Imperata cylindrica, the second was dominated by Leersia hexandra, and the third was dominated by Imperata cylindrica with importance values are 58.408 %, 72.202 %, and 81.316 %, respectively. The diversity index (H’) of grasses are 1.826, 1.432, 1.010 at area I, II, and III, respectively and equitability index (E) of grasses are 0.658, 0.597, 0.519 at area I, II, and III, respectively. The Similarity index of grasses ranges from 46.91% to 62.56%, with the highest similarity index is 62.56%  that is found between area II and III.   Keyword : Composition, grasses, Simalungun, structure.
KOMPOSISI DAN STRUKTUR RERUMPUTAN DI KAWASAN DANAU TOBA DESA HARANGGAOL KECAMATAN HARANGGAOL HORISON KABUPATEN SIMALUNGUN SUMATERA UTARA Novia Wulandari Tarigan; M Zaidun Sofyan; Nursahara Pasaribu
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

The composition and structure of Grasses in Haranggaol Village Haranggaol Horison Subdistrict Simalungun Regency North Sumatra has been studied from April to July 2012. The objective of the study was to determine the composition and structure of grasses at different altitudes. Sampling area was defined by purposive sampling method  and divided into three locations based on altitude with 20 plots of 1 x 1 m size for each area. From the study it was found 24 species of grasses that belong to two families. The first location was dominated by Microstegium ciliatum (81.095%), the second was dominated by Deyeuxia brachyathera (68.504%) and the third was dominated by Leersia hexandra (79.158%). The diversity index  (H')  of grasses are 1.557, 1.790 and 1.414 in the location I, II and III, respectively and equitability index (E) of grasses are 0.607, 0.661, 0.726 in the location I, II and III, respectively. The similarity index ranges from 30 to 50%, with the highest similarity index is 50% that was found between location I and II.   Keyword: composition, grass, Haranggaol, structure
ISOLASI DAN UJI POTENSI ISOLAT BAKTERI PELARUT FOSFAT DAN BAKTERI PENGHASIL HORMON IAA (Indole Acetic Acid) TERHADAP PERTUMBUHAN KEDELAI (Glycine max L.) PADA TANAH KUNING Dessy Merry Silitonga; Nunuk Priyani; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

Biofertilizer is a safe alternative fertilizer instead of various chemical fertilizers for increasing plant productivity which can minimize the ecological damage. There are plant symbiotic-soil bacteria which can solubilize phosphate or produce IAA. The objectives of this research are to determine the best soil bacteria in producing IAA or solubilizing phosphate and to know their roles in promoting plant growth in unfertile soil. As many as 8 isolates of phosphate solubilizing bacteria and 5 isolates of IAA producing bacteria were found. Among those isolates, there were 2 potential isolates (P2 & P4) that were able to solubilize phosphate and 1 potential isolate (I3) that was able to produce IAA. Isolate P4 produced holozone as wide as 1.45 cm and isolate P2 was 1.3 cm. Meanwhile, isolate I3 produced the highest concentration of IAA compared to the other isolates, that was 33.3 ppm. These isolates were applicated further on unfertile soil (yellow soil) as a medium for soybean growth. Soybeans have been grown for 10 weeks to observe the effect of those potential isolates on plant growth and productivity. The result showed that application of  isolate I3 was able to promote plant growth and increase plant productivity better than the other isolates as well as controls (fertile and unfertile soil). It promoted the soybean growth such as plant height was 58,8 cm; plant fresh weight was 3.5 g; plant dry weight was 1.55; pod fresh weight was 1.23; pod dry weight was 1.03; the amount of the pod was 3 and the number of the seed was 7.   Keywords : Phosphate solubilizing bacteria, IAA producing bacteria, biofertilizer, soybean.
SELEKSI BAKTERI PENAMBAT NITROGEN DAN PENGHASIL HORMON IAA (Indole Acetic Acid) DARI RIZOSFER TANAH PERKEBUNAN KEDELAI (Glycine max L.) Ratna Sari Tarigan; It Jamilah; Elimasni D
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

Rizosphere bacteria can be used to fix nitrogen and  produce IAA (Indol Acetic Acid) as biofertilizer to support plant growth. The ability of rhizosphere bacteria can be improved using biotechnology. The aim of this study is to select the most potential nitrogen fixing and IAA producing bacteria. Nitrogen fixing and IAA producing bacteria were isolated using JNFB and Luria Bertani + L-tryptofan medium respectively. The ability of nitrogen fixing bacteria were tested by ARA method, while the ability of IAA producing bacteria were investigated by spectrophotometer technique of 535 nm. The highest IAA concentration was produced by isolate I3 which was 33.3 ppm and the highest concentration of nitrogen was yielded by isolate N3 which was 29.93 ppm. Both N3 isolate and I3 isolate potentially as biofertilizer known as PGPR (Plant Growth Promoting Rhizobacteria).   Keywords: nitrogen fixing bacteria, IAA producing bacteria, PGPR
PENGARUH BUAH TERONG BELANDA (Solanum betaceum Cav.) TERHADAP JUMLAH ERITROSIT DAN KADAR HEMOGLOBIN MENCIT JANTAN (Mus musculus L.) ANEMIA STRAIN DDW MELALUI INDUKSI NATRIUM NITRIT (NaNO2) Sister Sianturi; Masitta Tanjung; Emita Sabri
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

The effect  of  terong belanda (Solanum betaceum Cav.) juice on anemia mice DDW strain induced by sodium nitrite has been studied from March 2012 to August 2012 in the Laboratory of Animal Physiology and Laboratory of Tissue Culture, Department of Biology, Faculty of Mathematics and Natural Science, University of Sumatera Utara, Medan. This research performed with completely randomized design and was divided in two controls and three treatments. The treatments were divided into three classes concentration of juice such as: 40% (P1), 50% (P2), and 60% (P3) respectively. Each class consisted of six animals. The juice was given orally 0.3ml/day. Results indicated that juice of terong belanda in anemia male mice showed no significantly increased  in the number of erythrocytes if compared to either the anemia control or blank control, but significantly increased  in haemoglobin levels if compared to anemia control.   Keywords : erythrocytes, haemoglobin, Mus musculus, sodium nitrit, terong belanda
KEANEKARAGAMAN POHON DAN POLE SERTA POTENSI KARBON TERSIMPAN DI KAWASAN HUTAN SEKUNDER 30 TAHUN DAN PERKEBUNAN KOPI DI TELAGAH, LANGKAT Jhon Marthali Simamora; Retno Widhiastuti; Nursahara Pasaribu
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

The diversity of tree, pole and potential of carbon stored in 30 years secondary forest and coffee field at Telagah District of Langkat was investigated from February to June 2012. The  study site was determined by purposive sampling method with total area 40 x 200 meters. The study site is divided into 20 plots and sub plots. Result found that 15 families and 23 species for tree phase and 16 families and 26 species of pole phase. The area were dominated by Macaranga tanaria with important value 57.11% for tree, and followed by Ficus aurantiacea with important value 54. 472% for pole. The diversity index of tree is 2.650 and 2.715 for pole. Equitability index of tree is 0.520 and 0.586 for pole. The potential of carbon stored in tree is 146.519 ton/ha, 3.933 ton/ha for pole, and 0.0489 ton/ha for coffee plantation.   Keywords: Carbon, Coffee Plantation, Diversity, Important value index, pole, purposive sampling
PENGARUH EKSTRAK SEGAR DAUN ROSELA (Hibiscus sabdariffa L.) TERHADAP JUMLAH ERITROSIT DAN KADAR HEMOGLOBIN MENCIT JANTAN (Mus musculus L.) ANEMIA STRAIN DDW MELALUI INDUKSI NATRIUM NITRIT (NaNO2) Asmitra Sembiring; Masitta Tanjung; Emita Sabri
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

The purpose of this research is to investigate the effect of fresh leave extract of rosela (Hibiscus sabdariffa L.) on erythrocyte and haemoglobin concentration in anemia male mice (Mus musculus L.) DDW strain induced by sodium nitrite. Completely randomized design (CRD) was used in this experiment, consisting of 5 groups. The first two groups is control induced by NaNO2 for 18 days and the second group is induced by NaNO2 for 18 days and then distilled water for 14 days, and three groups of teratment. The treatment group, divided into three classes of concentration such as; 14 mg/ml (P1), 28 mg/ml (P2) and 42 mg/ml (P3). The treatment group induced by NaNO2 first for 18 days and then healing process by extract of rosela leaves for 14 days. Each class consisted of six experiments animals. The extract was given orally     0.3 ml/day.  The results indicate that leave extract affect on increasing of erythrocytes and hemoglobin concentration that have a significantly different (p<0.05) between control and  treatments.   Keyword: anemic, erythrocytes, haemoglobin, rosela leaves.

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