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UJI TOKSISITAS SUBKRONIS EKSTRAK ETANOL DAUN TUMBUHAN SALA (Cynometra ramiflora Linn.) DENGAN PARAMETER KIMIA URIN DAN HISTOPATOLOGI ORGAN GINJAL PADA TIKUS GALUR WISTAR Haryoto -; Andi Suhendi; Eka Prasnaparamita W; Tanti Azizah Sujono; Muhtadi -
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Tumbuhan Sala (Cynometra ramiflorra Linn.) merupakan salah satu tumbuhan mangrove, yang memiliki beberapa manfaat antara lain antioksidan, antihiperglikemia, dan antiinflamasi. Uji toksisitas subkronis digunakan untuk mengetahui toksisitas suatu senyawa yang dilakukan selama 90 hari. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan ekstrak etanol daun Sala terhadap parameter kimia urin dan histopatologi ginjal. Penelitian ini merupakan penelitian eksperimental dengan rancangan Post Test Controlled Group Design, yaitu melihat perubahan yang terjadi pada tikus setelah diberi perlakuan. Sampel penelitian terdiri dari 20 ekor tikus putih jantan yang dibagi 4 kelompok dosis perlakuan. Kelompok pertama merupakan kelompok kontrol yang diberi CMC-Na (Sodium Carboxy Methyl Cellulose), kelompok kedua, ketiga, dan keempat diberi ekstrak etanol daun Sala dengan dosis 500, 1000, dan 1500 mg/kgBB. Parameter yang diamati yaitu kimia urin dan histopatologi ginjal. Parameter kimia urin dievaluasi menggunakan urin tampungan pagi hari pada hari ke-0, 45, dan 90 menggunakan reagen strip. Hasil parameter kimia urin tikus untuk kelompok kontrol dan perlakuan selama 90 hari memberikan pengaruh pada parameter protein, glukosa, keton, bilirubin, urobilinogen terhadap fungsi ginjal, namun tidak mempengaruhi pH dan nitrit. Hasil histopatologi menunjukkan terjadinya peradangan pada ginjal tikus yang diberi ekstrak etanol daun Sala dengan dosis 1000 dan 1500 mg/kgBB.Kata kunci: Cynometra ramiflora Linn., toksisitas subkronis, kimia urin
KADAR KALSIUM DAN FOSFOR DARAH PADA TIKUS YANG DIBERIKAN MOCAF TERFORTIFIKASI KALSIUM DARI CANGKANG TELUR AYAM RAS Wulandari Meikawati; Agus Suyanto
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

The most high calcium requirement occurs in adolescence than other age stages due to the rapid skeletal growth. The intake of calcium can be obtained from high-calcium foods or food ingredients that have been fortified with calcium. Mocaf is a product of flour from cassava (Manihot esculenta Crantz) is processed with the principle of modified cassava cell fermentation so that it has the color whiter than usual cassava flour and neutral flavor. Shell eggs is one of waste containing calcium is high enough, so that it can be used as fortifikan. This study aims to determine the levels of calcium and phosphorus in the blood of rats given standard feed ingredients coupled with mocaf fortified with calcium from the shells of eggs. Experimental animals divided into four, namely group 1 was given a standard feed (control group), group 2 was given a standard feed + mocaf fortified calcium from eggshell with a dose of 5%, group 3 was given a standard feed + mocaf fortified calcium from eggshell with a dose of 10% Group 4 was given the standard feed + CaCO3 at a dose of 5%. Treatment was given for 7 weeks. Blood sampling performed on the first and eighth week. The results showed the highest rates of blood calcium levels before treatment contained in the group 2 is 10.31 mg / dl and the lowest 9,78mg / dl in group 1. After the treatment is given the highest rates of blood calcium levels found in the group 3 is 12.04 mg / dl and the lowest 9.95 mg / dl in group 1. There is the effect of calcium on the eggshell of the blood calcium level, and there are differences in the level of calcium in the blood before and after treatment. There are differences in phosphorus levels before and after treatment, but there was no difference between treatment groups phosphorus levels.Keywords: Calcium, Phosphorus, Rats, MOCAF
EMERGENCE RESISTANT UROPATOGEN Escherichia coli SETELAH PEMBERIAN SIPROFLOKSASIN DAN -MANGOSTIN SECARA in vitro Maya Dian Rakhmawatie; Afiana Rohmani
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Emergence resistant on uropatogen Escherichia coli can occur shortly after the start of therapy using subtherapeutic doses of ciprofloxacin. Ciprofloxacin is an antibiotic that works depends on the level of concentration, higher ratio Cmax / MIC will give increases in effectiveness. When the ratio of Cmax / MIC <1, then the risk of emergence resistant will be increased. One of the herbs that are abundant in Indonesia and has anti-bacterial activity is mangosteen (Garcinia mangostana L.), which has an active compound -mangostin. Administration of the active compound α-mangostin is expected to help prevent the emergence resistant of uropatogen E. coli due to the use of subtherapeutic ciprofloxacin. This research was conducted by giving treatment to uropatogen E. coli in vitro. Bacterial strains used are uropatogen E. coli resistant to ciprofloxacin with MIC values of 128 μg / mL. Treatment is divided into (I) treatment groups using ciprofloxacin concentration Cmax at a dose of 750 mg (4.3 μg / mL), (II) treatment groups using ciprofloxacin concentration of 4.3 μg / mL and -mangostin 0.18 μg / mL, and (III) the negative control group. The study states that the administration of the combination of α-mangostin and ciprofloxacin delayed the growth of uropatogen E. coli resistant strains (MIC value of 128 μg / mL) compared to administration of ciprofloxacin alone (p 0.000). But the combination of α-mangostin and ciprofloxacin can not prevent an increased in resistance strain uropatogen E. coli, which is characterized by an increased in the value of the MIC to be 256 μg / mL after 2 hours of treatment.Keywords: ciprofloxacin, resistance, -mangostin, uropatogen E. coli
FAKTOR-FAKTOR YANG BERHUBUNGAN DENGAN KADAR ASAM URAT DALAM DARAH PASIEN GOUT DI DESA KEDUNGWINONG SUKOLILO PATI Sukarmin -
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Di Indonesia, asam urat menduduki urutan kedua setelah osteoartritis. Namun, di Indonesia prevalensi penyakit asam urat belum diketahui secara pasti dan cukup bervariasi antara satu daerah. Survei pendahuluan pada tanggal 2-4 Juni 2014, melalui Klinik DesaDi Desa Kedungwinong Kecamatan Sukolilo Pati dalam satu bulan rata-rata ada 35 orang yang mengalami penyakit gout. Tujuan penelitian ini adalah mengidentifikasi faktor-faktor yang berhungan dengan kadar asam urat dalam darah pasien gout di Desa Kedungwinong Sukolilo Pati. Jenis penelitian ini adalah korelasi. Sampel penelitian ini adalah pasien gout di Desa Kedungwinong yang menurut data Puskesmas Pembantu Sukolilo berjumlah 35. Hasil Penelitian. Rata-rata usia responden 52.6 tahun, mayoritas responden berjenis kelamin wanita (24 orang / 68,8%), mayoritas pekerjaan petani (26 orang / 74,3%), mayoritas reponden mempunyai riwayat keturunan asam urat (25 orang / 71,4%), mayoritas mengkonsumsi obat-obatan beresiko asam urat (29 orang / 82,9%), mayoritas mengkonsumsi diet tinggi purin (29 orang / 82,9%). Analisa chi square menunjukkan hasil adanya hubungan antara adanya hubungan antara faktor keturunan dengan kadar asam urat (p : 0.03. α : 0.05), tidak adanya hubungan antara konsumsi obat beresiko asam urat dengan kadar asam urat (p:0,63), adanya hubungan antara diet dengan kadar asam urat (0,012).Kata kunci : Gout, Keturunan, Konsumsi Obat Resiko Asam Urat, Diet, Kadar Asam Urat
POLA DYSMENORRHEA PRIMER PADA REMAJA DI MAN 1 SEMARANG Ulfatul Mardhiyah; Ali Rosidi; Indri Astuti Purwanti
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Menstruation is a sign of a primary sex for women. The most common menstrual disorders is dysmenorrhea. The average incidence of dysmenorrhea in Indonesia is about 55% of which consists of a primary dysmenorrhea (54.89%) and secondary dysmenorrhea (9.36%). Primary dysmenorrhea happened in MAN 1 Semarang as much as 27%. This research objects to describe the patterns of the primary dysmenorrhea in MAN 1 Semarang. The study was conducted by survey method and approach of cross-sectional. Respondents were selected X-grader is experiencing Dysmenorrhea. Sampling technique is simple random sampling and numbers of sample are 46 respondents. The results showed that most respondents always experiencing dysmenorrhea (65.2%), while respondents perceived dysmenorrhea symptoms are pain in the lower abdomen that extends to the back and leg (91.3%), sore on the leg (73.9 %), and sore at the waist (76.1%). Dysmenorrhea interval that occurred more than 3 years after menarche (47.8%) more than 2-3 years (39.1%) and less than 2 years (13%).Keywords: primary dysmenorrhea, menstrual disorder, teenager.
PENGETAHUAN DAN PERILAKU PASIEN TUBERKULOSIS TERHADAP PENYAKIT DAN PENGOBATANNYA Hidayah Karuniawati; Arifah Sri Wahyuni; Heni Mirawati; Suryani -; Sulistyarini -
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Tuberculosis is the most problem in health care and leading the first death in infection disease in Indonesia. Knowledge and behavior in fluent to success in medication tbc. The purpose of this study was to understanding knowledge and behavior patient tuberculosis about their disease and medication. This study was descriptive approach, collecting data had been done by interview and questioner to patient tuberculosis, and clarification to medical record and medical staff. Result showed that respondents knowledge about their disease is 83% in high category and 17% in moderate category, respondents knowledge about their medicinal treatment is 97% in high category and 3% in moderate category. Respondents know about their disease, symptoms, and how the disease spread or infect to another, and know how to prevent spreading of this disease. Respondents know that tbc can be cured and the length of medication needs for several months. Being heal, respondents do disciplinary to taking their medication.Keywords : tbc, knowledge, behavior, disease, medication
PENGARUH PELATIHAN SENAM HAMIL TERHADAP PENINGKATAN KETRAMPILAN SENAM HAMIL DI WILAYAH KERJA PUSKESMAS KEDUNGMUNDU SEMARANG Rose Nurhudhariani; Siti Nur Umariyah Febriyanti; Vita Triani Adi Putri
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

The application of pregnant mother class is needed for high risk pregnancy. The materials that was taught in pregnant mother class such as Prenatal Excercise. The benefit of doing Prenatal Exercise is helping a pregnant mother to deliver their baby normally, maintaining body readiness, helping to maintain mom’s & baby’s health, giving a relaxed mind and creating a good mechanical system for baby in and after pregancy. This research was aimed to analize the influence of Prenatal Exercise traininig with Prenatal Exercise skill at Kedungmundu District Health Centre.this research used quantitative design with pre-post test without control group approach. The populations were all pregnant mothers at Kedungmundu District Health Centre, and took samples were 15 pregnant mothers by using accidental sampling technique with Wilcoxon to analize the development of skill before and after Prenatal Exercise training. The result showed the average of rate skill before training was 6,20 with Deviation Standard 1,93. And the rate skill after given training was 8,30 with Deviation Standard 1,52 with p value 0,000 < 0,05. The conclusion is the Prenatal Exercise training with lecturing and demosntration is efective to increase skill of Prenatal Exercise in pregnan mothers at Kedung Mundu District Health Center Semarang.Key Words : Prenatal Exercise, Pregnant Mothers
KADAR YODIUM AIR, EKSKRESI YODIUM URIN DAN GOITER DI DAERAH ENDEMIS DEFISIENSI YODIUM Mutalazimah -; Setia Asyanti
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Physical environmental factors contribute to human health, including the factor of iodine content of water in endemic areas related to urinary iodine excretion and goiter. Water iodine levels determine iodine intake comes from drinking water and determine the iodine content of foodstuffs produced from endemic areas. When iodine intake is too low, then the amount of iodine excreted in the urine is also low, it causes the thyroid gland is unable to maintain adequate hormone secretion, and goiter arise as compensation. This study aims to examine the relationship of water iodine levels, urinary iodine excretion (UIE) and goiter in women of childbearing age in endemic areas of iodine deficiency. This cross-sectional observational study, conducted in 115 women of childbearing age at Prambanan sub-district of Sleman, chosen at random. Water iodine levels and UIE were measured by acid digestion method, goiter measured by palpation method. The relationship between water iodine levels and UIE were analyzed with the Pearson correlation test, the relationship of water iodine levels and goiter were analyzed by Chi Square. The results showed that the average iodine content of water 4.95 mg / l ± 4.98 mg/l and median 8.00 mg/l (0-12 mg l), the average value of UIE included in the category more adequate (247.3 ± 158.5) mg/l and it also the median of 205 (0-774) mg/l. It was also found 13% sample have goitre. Statistical analysis result showed the relationship between water iodine levels and UIE (p = 0.014), while water iodine levels and goiter (p = 0.077). In conclusionthere was athere relationship between water iodine levels and UIE and there was no correlation between water iodine levels and goiter.Keywords: water iodine levels, UIE, goiter
EFEKTIVITAS PENERAPAN POLA ASUH SEHAT MENTAL TERHADAP PERKEMBANGAN PSIKOSOSIAL ANAK PRASEKOLAH USIA 3-6 TAHUN Susana Nurtanti; Nita Yunianti R
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

The background of this research is the increasing number of child abuse committed by parents and those nearest that hinder the psychosocial development. The purpose of this study was to analyze the effectiveness of mental health parenting to the psychosocial development of pre-school age children 3-6 years in TK Pertiwi I Pracimantoro. This study uses a study design one group pre post test design. The samples were all parents in TK Pertiwi I who have children aged three to six years some 23 peoples. The dependent variable in this study is mental health parenting, the independent variable is the psychosocial development of preschool children 3-6 years. The research instrument used was a questionnaire. Data analysis using SPSS version 17 for analyzing the characteristics of respondents by age, gender, occupation and number of children, to analyze the effect of mental health parenting to psychosocial development of children aged 3-6 years with T test. The results show the value of t is greater than t table so that there is a significant effect. p-value 0.000 > 0.05 (95% confidence level) showed differences in the psychosocial development before and after the application of mental health parenting. The mean result is 18 174 before treatment, after treatment is 20 478 there is a difference score of 2.304 indicating an increase in psychosocial development of preschool children 3 – 6 years after getting treatment of mental health parenting of parents.Keywords: Mental Health Parenting, Psychosocial Development, Preschool children age 3-6 years.
KEANEKARAGAMAN DAN POTENSI MAKROFUNGI TAMAN NASIONAL GUNUNG MERAPI Aniek Prasetyaningsih; Djoko Rahardjo
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Makrofungi atau jamur yang memiliki tubuh buah merupakan komponen utama dalam ekosistem terestrial dan mempunyai kontribusi penting terhadap ekosistem. Taman Nasional Gunung Merapi (TNGM), sebagai sebuah taman nasional yang relatif baru ditetapkan (2004) dan sangat dipengaruhi oleh aktivitas erupsi merapi, menjadi hal yang menarik mengkaji keanekaragaman makrofunginya baik di lereng selatan maupun utara untuk mengetahui keragaman jenis, distribusi, pemanfaatan dan potensi pengembangannya serta strategi konservasinya. Penelitian dilakukan mulai bulan Maret 2013 hingga Desember 2014, di dua kawasan TNGM yaitu lereng selatan (Telaga Muncar, Telaga Nirmolo dan Turgo) dan lereng utara (Selo dan Stabelan). Penelitian dilakukan dalam tiga tahapan yaitu observasi awal untuk menentukan jalur observasi dan plot untuk penelitian, pengambilan sampel serta pemetaan distribusi makrofungi, dan identifikasi makrofungi. Deskripsi makromorfologi mengikuti deskripsi Bolete and Agaric annotation sheet (B.Ortiz-Santana and D.J. Lodge and Cantrell) Lodge et.al. 2004 dalam Mueller, 2004., sedangkan deskripsi mikromorfologi dilakukan dengan mengamati kharateristik spora (bentuk, warna dan ukuran) Untuk pengklasifikasian digunakan Dictyonary of the Fungi oleh : Kirk, et all. 2008 dan Buku Morphology of Plants and Fungi oleh Bold et all., 1909. Uji potensi senyawa aktif dilakukan dengan metode HTS. Data yang diperoleh dianalisis secara deskriptif-kualitatif untuk menjelaskan hubungan antara keragaman, sebaran serta potensi masing-masing spesimen yang ditemukan dan keterkaitannya dengan tipe vegetasinya. Dari hasil observasi dan identifikasi, untuk lereng selatan kawasan TNGM ditemukan 129 spesies, 122 spesies diantaranya teridentifikasi dan 7 spesies sisanya belum teridentifikasi. Dari spesies yang teridentifikasi, dikelompokan kedalam 14 ordo dan 41 famili, sementara untuk lereng utara hanya ditemukan 37 spesies. Spesies yang ditemukan didominasi dari ordo Agaricales dan ordo Aphyllophorales. Keragaman makrofungi paling banyak ditemukan di kawasan Lereng Selatan dibanding dengan Lereng Utara, dan faktor yang paling berpengaruh adalah tipe vegetasi, dan ketersediaan bahan organik bagi pertumbuhan makrofungi. Beberapa spesies makrofungi dari famili Mycenaceae, Polyporaceae, Arauculariaceae dan Tremellaceae memiliki potensi sebagai antibakteri, sedangkan beberapa jenis dari ordo Polyporaceae berpotensi sebagai antioksidan. Bahkan untuk Ganoderma applanatum merupakan spesies yang paling potensial untuk digunakan sebagai biokontrol terhadap bakteri Xanthomonas oryzae pv. Oryzae ; R.s : Ralstonia solanacearum ; P.c: Pectobacterium carotovorum, dengan nilai MIC 1 mg/ml. a dan terdistribusi secara spesifik sesuai dengan karakteristik lingkungan.Keyword : HTS (High Troughput Screening).makrofungi, makromorfologi, TNGM

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