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Jurnal Bioteknologi & Biosains Indonesia
Published by Badan Riset dan Inovasi Nasional
ISSN : 24422606     EISSN : 2548611X     DOI : https://doi.org/10.55981/jbbi
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general. JBBI terbit dua kali setahun dan menyediakan media penerbitan ilmiah bagi para peneliti, insinyur, praktisi, akademisi, dan pemerhati di bidang yang terkait dengan bioteknologi dan biosains. Jurnal ini menerima naskah asli hasil penelitian, naskah ulasan, studi kasus, dan komunikasi singkat. Naskah yang diterbitkan adalah peer-review oleh tidak kurang dari dua orang penelaah, dan mencakup berbagai cabang bioteknologi yang terkait dengan bidang pertanian, industri, kesehatan, lingkungan, bioinformatika, serta ilmu kehidupan pada umumnya.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 17 Documents
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Search results for , issue "Vol. 11 No. 2 (2024)" : 17 Documents clear
UTILIZATION OF PINEAPPLE PROCESSING LIQUID WASTE IN BIODEGRADATION OF DISPOSABLE FACE MASK BY BACTERIA FROM LAMPUNG BAY Deviany, Deviany; Zhofiroh, Nawrah Meisyah Muthi'ah; Febrina, Yane Fita; Yusuf, Reggina Aulia; Achmad, Feerzet; Yuniarti, Reni; Istiadi, Khaerunissa Anbar
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8192

Abstract

The designation of COVID-19 as a global pandemic led to an increased use of single-use face masks, which result in waste that is difficult to degrade and has the potential to release microplastic fibers into the environment. This study aims to examine the im-pact of adding pineapple peel liquid waste (LCN) as a growth medium for the biodeg-radation of single-use face masks by bacterial isolates obtained from the waters of the Lampung Bay. The study investigates how LCN affects the efficiency of mask degra-dation by microorganisms, as well as how the ratio of LCN mixed with other growth media, such as Nutrient Broth (NB), influences the degradation process. The biodeg-radation process was carried out using a biostimulation technique, where bacterial iso-lates were incubated in media containing LCN at a 1:1 ratio. The degradation process lasted for 15 days, with the results being analyzed using gravimetry and Fourier-Transform Infrared Spectroscopy (FTIR). Gravimetric results showed a greater weight reduction in treated masks compared to the control masks. FTIR analysis also indicat-ed changes in the intensity of functional groups in the degraded layers of the masks, as well as the emergence of C≡C functional groups in the second and third layers. This study demonstrates that the addition of LCN can accelerate the biodegradation of sin-gle-use face masks, offering a new approach for managing mask waste.
THE ANTI-APOPTOTIC POTENTIAL OF Paederia foetida L. LEAF EXTRACT THROUGH THE DOWNREGULATION OF CASPASE-3 EXPRESSION IN AN ESCHERICHIA COLI-INDUCED SEPSIS MICE MODEL Savitri, Lisa; Krissanjaya, Rochmad; Juwita, Syntia Tanu; Sukmawati, Datin An Nisa; Probosiwi, Neni; Laili, Nur Fahma; Ilmi, Tsamrotul; Prasetyawan, Fendy
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8235

Abstract

Sepsis is a life-threatening condition characterized by an abnormal immune response to infection, leading to high mortality rates in intensive care units (ICUs) worldwide. Caspase-3, a crucial enzyme in the apoptosis pathway, plays a significant role in sep-sis-related cellular damage. This study investigates the anti-apoptotic potential of Paederia foetida L. leaf extract by examining its effect on Caspase-3 expression in an Escherichia coli-induced sepsis mice model. Male Balb/c mice were divided into six groups, including positive control, negative control, and treatment groups receiving varying doses of the extract (100 mg/kgBW, 300 mg/kgBW, and 500 mg/kgBW). Caspase-3 expression in the spleen was measured after 24 hours of treatment. The results demonstrated a significant reduction in Caspase-3 expression, particularly in the group treated with 500 mg/kgBW of the extract, indicating its anti-apoptotic effect. These findings suggest that P. foetida leaf extract may serve as a potential therapeutic agent for reducing cell apoptosis in sepsis, warranting further investigation into its mechanisms and clinical pharmacological field.
UTILIZATION OF FISH PROTEIN HYDROLYSATE AS A NITROGEN SOURCE IN FERMENTED MEDIA OF CEPHALOSPORIN C PRODUCTION BY ACREMONIUM CHRYSOGENUM Biomcc 00141 Fatonah, Ani Fatonah; Saepudin, Endang; Suyanto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8237

Abstract

Optimizing Indonesia's maritime resources as an effort to reduce reliance on imported raw materials for the pharmaceutical industry. This study investigates the use of fish protein hydrolysate, derived from trash fish flour, as a supplement in the fermentation process for producing cephalosporin C. This key raw material is used to create cephalosporin antibiotics through fermentation with the fungus Acremonium chrysogenum Biomcc 0141. Hydrolysate from trash fish flour is obtained through an enzymatic hydrolysis process using a protease found in pineapple hump extract which was optimized. The results of hydrolysis optimization on hydrolysate from trash fish flour produce a protein content of 3.37%. The protein hydrolysate obtained is then used in the cephalosporin C fermentation process. The addition of trash fish protein hydrolysate to cephalosporin C fermentation media showed a significant increase in the productivity of cephalosporin C, which is up to 85.39% higher than the fermentation process without the addition of fish protein hydrolysates.
IN SILICO ANALYSIS OF SMALL INTERFERING RNA TARGETING THE NUCLEOPROTEIN GENE OF INFLUENZA VIRUSES Hartawan, Risza; Ratnawati, Atik; Sendow, Indrawati; Dharmayanti, Ni Luh Putu Indi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8521

Abstract

Small interfering RNA (siRNA) is a promising therapeutic against viral infection, includ-ing Influenza viruses. However, the Influenza viruses have massive variants with high mutation rates. Therefore, the siRNAs could be futile against newly emerging viruses. Thus, this study aimed to analyze siRNAs targeting the nucleoprotein gene of Influen-za viruses. Using bioinformatic analyses, the siRNAs were simulated against 5 sub-types of Influenza viruses, including H1N1, H3N2, H5N1, H7N9, and H9N2. Bioinfor-matic tools for the folding structure of messenger RNA were utilized to select effective siRNA. As a result, 32 siRNA sequences targeting the nucleoprotein gene were identi-fied. The precision medicine concept seems applied to the siRNA treatment for the In-fluenza virus since each siRNA is effective in its respective virus target. Based on the nucleotide mismatch parameter, most siRNA does not have coverage for the multiple infections of all five subtypes of Influenza viruses, except for NP1089 and NP1496. Later, the secondary and tertiary structure analysis of messenger RNA demonstrated that siRNA has different circumstances in its RNA target position. Therefore, siRNA mapping based on the RNA folding structure approach provides a tool for selecting more effective sequences against Influenza virus infection. Both siRNA NP1089 and NP1496 were predicted to have similar effectivity in knocking down Influenza virus in-fection. Moreover, the cocktail application of siRNA treatment may be effective as an alternative strategy in matching co-infection of multiple Influenza virus subtypes.
UTILIZATION OF KECOMBRANG FRUIT PEEL (Etlingera elatior (Jack) RMSm) AS A MOUTHWASH TO PREVENT MOUTH ULCERS AND DENTAL CARIES Rahmadina, Rahmadina; Mayasari, Ulfayani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8591

Abstract

Kecombrang fruit is used by the community as a food ingredient but actually the skin of this kecombrang fruit can be used as a mouthwash for the development of dental caries. This study aims to evaluate the preparation of mouthwash formulations with the addition of kecombrang fruit peel extract. The work procedure starts from extraction, phytochemical testing, antimicrobial testing of extracts, and evaluation of mouthwash preparations. The results contained secondary metabolites in the form of alkaloids, fla-vonoids, tannins, and saponins. Evaluation of mouthwash preparation with pH test is 5.0-5.6, organoleptic test which does not change significantly, viscosity test at 20% and 30% concentration is 1.0193 cP and 1.0061 cP, and antimicrobial test with inhibi-tion diameter on Streptococcus mutans and Candida albicans is 11.3 mm and 11.1 mm. In conclusion, mouthwash with added extracts can inhibit the growth of microbes that cause mouth ulcers and tooth decay.
THE EFFECT OF Paederia foetida L. EXTRACT ON LIVER WEIGHT OF MICE SEPSIS MODEL INFECTED WITH Escherichia coli Savitri, Lisa; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8965

Abstract

Sepsis is a critical medical condition characterized by a systemic immune response to in-fection, which can lead to severe organ dysfunction and mortality. Bacterial sepsis, par-ticularly caused by Escherichia coli, poses a significant risk due to its potential to harm tissues and organs, including the liver. The liver plays a central role in metabolic process-es and host defense during sepsis, making it a key organ of interest. This study aimed to investigate the effect of Paederia foetida leaf extract on liver weight in a mice sepsis mod-el. Mice were divided into six groups: normal control (N), negative control (K-) receiving distilled water, positive control (K+) receiving ciprofloxacin, treatment 1 (P1) receiving P. foetida extract at 100 mg/kg BW, treatment 2 (P2) receiving 300 mg/kg BW, and treat-ment 3 (P3) receiving 500 mg/kg BW. After 14 days of treatment, significant differences in liver weight were observed among the groups, with the highest mean and standard de-viation recorded in the P1 group (1.3750 ± 0.3932). Liver abnormalities, including swell-ing, lobular thickening, and weight increase, were identified, indicating the liver’s adap-tive response to toxic substances during sepsis. Interestingly, the normal control group exhibited higher liver weights compared to the treatment groups, possibly due to fatty substance accumulation within the liver tissues. These findings suggest that P. foetida ex-tract may influence liver weight changes in sepsis, potentially modulating metabolic and detoxification processes. Further studies focusing on histopathological and biochemical mechanisms are needed to clarify the therapeutic potential of P. foetida in managing liver dysfunction associated with sepsis.
THE POTENCY OF BUNIDA F1 AND THEIR CROSSBREED (BUNIDA F2) AS SUPERIOR LOCAL CHICKEN Rahmi, Annisa; Baharun, Abdullah; Adinda Putri, Tivanka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8978

Abstract

High market demands for animal protein have encouraged the optimization of local chicken genetic resources. This research aims to determine the potency of BUNIDA F1 chickens and their crossed-breed (BUNIDA F2). Five BUNIDA F1 hens at 12 months of age are inseminated with Bresse’s semen until they get 50 eggs. The eggs are incubated at 37-38°C and 60-70% humidity. The egg’s fertility and embryo devel-opment were observed on the 4th, 7th, and 18th days of incubation with the candling method. The parameters observed were egg weight, egg fertility, hatchability, and hatching weight. The results show BUNIDA F1 potencies are 54.12 ± 2.70 g egg weight, 82.59 ± 5.11% fertility rate, 71.14 ± 10.67% for hatchability, and hatching weight of BUNIDA F2 is 37.86 ± 1.97 g. It can be concluded that BUNIDA F1 and BUNIDA F2 have the potency to be developed into superior local chickens.

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