Garuda - Garba Rujukan Digital

Potensi Senyawa Aktif Mengkudu (Morinda citrifolia) sebagai Antifungi Candida albicans melalui Pendekatan in Silico Khaerunissa Anbar Istiadi; Erma Suryanti; Dewi Chusniasih
Wahana-Bio: Jurnal Biologi dan Pembelajarannya Vol 15, No 1 (2023): Wahana-Bio Edisi Mei 2023
Publisher : Program of Biology Education, Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/wb.v15i1.15883

Candida albicans is a normal microbiota in humans. However, the imbalance and increasing number of C. albicans can cause infections in the vagina, mouth, esophagus, and nails. Sap-3 plays role in the infections especially in the adhesion process, and can used as a therapeutic target for C.albicans. Here an attempt has been made to analyze the potential of the active compounds from noni (Morinda citrifolia) as an antifungal by molecular docking towards Sap-3 protein. Drug-likeness characteristics were analyzed based on the Lipinski Rule of Five. Validation of molecular docking of Sap-3 protein-native ligand was conducted followed by molecular docking of the Sap3 protein-test based on the Lamarckian Genetics Algorithm. The highest binding energy is Beta-sitosterol (-8.77 kcal/mol), followed by Alizarin (-6.47 kcal/mol), Morindone (-6.51 kcal/mol), Quercetin (-5.76 kcal/mol), Pepstatin A (-5.55 kcal/mol), Scopoletin (-5.26 kcal/mol), Kaempferol (-5.13 kcal/mol). Bioactive compounds from noni (Morinda citrifolia) can be an appropriate choice as antifungal and for further experiments through in vitro and in vivo tests.

Pelatihan Pemanfaatan Limbah Perkebunan Kelapa Sawit sebagai Bahan Baku Baglog Budidaya Jamur Tiram di Desa Sabah Balau, Lampung Selatan Khaerunissa Anbar Istiadi; Erma Suryanti; Hida Arliani Nur Anisa; Arysca Wisnu Satria; Deviany Deviany; Yuni Sukmawati; Dini Meilinda; Andreas Dwi Nugroho; Triya Yuli Andini; Aji Hafidz Talaga
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 4 (2023)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/jb.v4i4.6601

Kelapa sawit sebagai salah satu komoditas perkebunan unggulan di Indonesia menghasilkan limbah berupa pelepah selama proses produksi kelapa sawit. Desa Sabah Balau, Lampung Selatan merupakan kawasan yang dikelilingi oleh perkebunan karet sebagai komoditas utama serta tanaman kelapa sawit yang diusahakan masyarakat sekitar. Pengolahan limbah pelepah sawit berpeluang menyelesaikan permasalahan lingkungan serta menghasilkan nilai ekonomi bila diproses menjadi produk yang bernilai tinggi, yaitu media tanam jamur tiram (baglog). Pemberian informasi mengenai potensi limbah pelepah kelapa sawit di Desa Sabah Balau dilakukan melalui Pengabdian Kepada Masyarakat yang terdiri dari empat tahap, yaitu tahap perencanaan dan persiapan, tahap pelaksanaan, serta tahap evaluasi. Tahap pelaksanaan kegiatan dilakukan melalui sosialisasi dan praktik. Materi sosialisasi terintegrasi dengan praktik meliputi bahan baku dan produksi baglog, proses sterilisasi dan inokulasi serta cara budidaya jamur tiram yang baik. Kegiatan pengabdian kepada masyarakat yang telah dilakukan di Desa Sabah Balau berupa sosialisasi dan praktik pembuatan media tanam jamur tiram (baglog) meningkatkan pengetahuan masyarakat mengenai potensi limbah pelepah kelapa sawit sebagai bahan baku baglog, proses pembuatan baglog, serta proses budidaya jamur tiram.

Pelatihan Pemanfaatan Limbah Perkebunan Kelapa Sawit sebagai Bahan Baku Baglog Budidaya Jamur Tiram di Desa Sabah Balau, Lampung Selatan Khaerunissa Anbar Istiadi; Erma Suryanti; Hida Arliani Nur Anisa; Arysca Wisnu Satria; Deviany Deviany; Yuni Sukmawati; Dini Meilinda; Andreas Dwi Nugroho; Triya Yuli Andini; Aji Hafidz Talaga
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 4 (2023)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/jb.v4i4.6601

Kelapa sawit sebagai salah satu komoditas perkebunan unggulan di Indonesia menghasilkan limbah berupa pelepah selama proses produksi kelapa sawit. Desa Sabah Balau, Lampung Selatan merupakan kawasan yang dikelilingi oleh perkebunan karet sebagai komoditas utama serta tanaman kelapa sawit yang diusahakan masyarakat sekitar. Pengolahan limbah pelepah sawit berpeluang menyelesaikan permasalahan lingkungan serta menghasilkan nilai ekonomi bila diproses menjadi produk yang bernilai tinggi, yaitu media tanam jamur tiram (baglog). Pemberian informasi mengenai potensi limbah pelepah kelapa sawit di Desa Sabah Balau dilakukan melalui Pengabdian Kepada Masyarakat yang terdiri dari empat tahap, yaitu tahap perencanaan dan persiapan, tahap pelaksanaan, serta tahap evaluasi. Tahap pelaksanaan kegiatan dilakukan melalui sosialisasi dan praktik. Materi sosialisasi terintegrasi dengan praktik meliputi bahan baku dan produksi baglog, proses sterilisasi dan inokulasi serta cara budidaya jamur tiram yang baik. Kegiatan pengabdian kepada masyarakat yang telah dilakukan di Desa Sabah Balau berupa sosialisasi dan praktik pembuatan media tanam jamur tiram (baglog) meningkatkan pengetahuan masyarakat mengenai potensi limbah pelepah kelapa sawit sebagai bahan baku baglog, proses pembuatan baglog, serta proses budidaya jamur tiram.

A Computational Exploration: Docking Analysis of Compounds from Foeniculum vulgare as Potential Aromatase Inhibitors for Endometriosis Candidate Therapy Suryandari, Dwi Anita; Sari, Puji; Sunaryo, Hadi; Istiadi, Khaerunissa Anbar
Indonesian Journal of Medical Chemistry and Bioinformatics
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Aromatase inhibitors (AI) have controlling symptoms and size of endometriotic implants, making them a promising second-line therapy for endometriosis treatment.pretreatment with letrozole, an AI, combined with leuprolide acetate and resveratrol has been found to improve in vitro fertilization (IVF) outcomes in women mild endometriosis.in this study we screening and analysis of ten phenolic compounds from Foeniculum vulgare using molecular docking with Mcole server.from this results showed that three phenolic trans resveratrol (TR), caempherol coumaril (CC) have low gibbs energy compare with resveratrol (R). The binding modalities of compound TR and compound R were hydrogen-bonding between the hydroxyl and oxygen atom and Thr310 and hydrophobic interactions with Phe187, Ala272, Asp275, Ala189.and compound R exhibited cation-π interactions between Val336 as binding activity from aromatase.aromatase inhibitors and resveratrolfrom fennel lies in the potential of resveratrol to modulate hormonal pathways, including aromatase inhibition.

Molecular Insights into Propylthiouracil as a Thyroid Peroxidase Inhibitor: A Computational Study Approach Suryandari, Dwi Anita; Yunaini, Luluk; Sunaryo, Hadi; Istiadi, Khaerunissa Anbar; Pratomo, Irandi Putra
Indonesian Journal of Medical Chemistry and Bioinformatics
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Thyroid peroxidase (TPO) is a crucial enzyme in the biosynthesis of thyroid hormones, catalyzing the iodination of tyrosine residues in thyroglobulin and the coupling of iodotyrosines to form thyroxine (T4) and triiodothyronine (T3). Propylthiouracil (PTU) is an antithyroid drug commonly used to manage hyperthyroidism by inhibiting TPO. Understanding the molecular interactions between TPO and PTU can provide insights into the inhibitory mechanisms and guide the design of more effective antithyroid medications. Objective: This study aims to elucidate the binding interactions between TPO and PTU through molecular docking, providing a detailed understanding of how PTU inhibits TPO activity. Methods: The three-dimensional structure of TPO was obtained from Prosite and modelling by swissmodel and prepared for docking. The structure of PTU was optimized, and molecular docking was performed using AutoDock. The binding affinity, binding poses, and key interactions between TPO and PTU were analyzed. Visualization of the docking results was performed using PyMOL to identify critical residues involved in PTU binding. Results: The docking analysis revealed that PTU binds effectively to the active site of TPO with a binding affinity of -5.45 kcal/mol. The interaction involves coordination with the heme group and several key residues, including His239, which coordinates the heme, and Ser314, which forms hydrogen bonds with PTU. Additionally, hydrophobic interactions with residues Phe241 and Ile399 stabilize the binding of PTU in the active site. Conclusion: The docking study highlights the significant interactions between PTU and TPO, elucidating the molecular basis of TPO inhibition by PTU. The binding affinity and key interactions identified in this study provide a foundation for the design of more potent antithyroid drugs.

Perbedaan Profil Protein Plasma Darah Mencit Menggunakan SDS-PAGE Tanpa Penambahan dan dengan Penambahan 2-Mercaptoethanol Istiadi, Khaerunissa Anbar; Khairani, Iffa Afiqa; Andriani, Silvia
Jurnal Pendidikan Biologi Undiksha Vol. 11 No. 2 (2024)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v11i2.82139

SDS-PAGE is a method used to analyze proteins based on differences in molecular weight. Sample preparation in SDS-PAGE with 2-mercaptoethanol will reduce disulfide bonds in proteins. The sulfide bond reduction process will cause differences in protein conformation and profile. The purpose of this study was to compare the pattern of blood plasma protein profiles of mice analyzed using SDS-PAGE, with the addition of 2-mercaptoethanol and without the addition of 2-mercaptoethanol in a mixture of sample buffer solutions. The results of SDS-PAGE showed that the blood plasma protein profile of mice without the addition and with the addition of 2-mercaptoethanol showed differences in the number of protein bands, the molecular weight of several proteins and the thickness of the protein bands. There were nine protein bands with a molecular weight above 52 kDa in the profile without the addition of 2-mercaptoethanol, while there were only five protein bands with a molecular weight above 52 kDa in the profile with the addition of 2-mercaptoethanol. The difference in protein profile patterns shows that mouse blood plasma contains proteins containing disulfide bonds that can be reduced by 2-mercaptoethanol.

Pelatihan Pembuatan Pupuk Kompos di Desa Ngestirahayu dalam Upaya Kemandirian Pertanian Maretta, Gres; Istiadi, Khaerunissa Anbar; Wibawa, Fajri Arif; Budiono, Desi; Fatriani, Rizka; Hariyandi, Yopi; Astuti, Ayu Widia; Ferdinanda, Thierry; Aniesti, Frigia Rafilia
Lumbung Inovasi: Jurnal Pengabdian kepada Masyarakat Vol. 9 No. 4 (2024): December
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/linov.v9i4.2355

Desa Ngestirahayu merupakan salah satu Desa yang terletak di Kecamatan Punggur dengan mayoritas masyarakat Desa Ngestirahayu berprofesi sebagai petani. Selama ini, petani mengalami permasalahan salah satunya adalah ketersediaan dan supply pupuk subsidi yang dibatasi. Oleh sebab itu, diperlukan alternatif pupuk yang murah dan dapat diproduksi secara mandiri. Selain itu, pupuk organik dapat memperbaiki kualitas tanah dan menyediakan nutrisi esensial bagi tanaman secara lebih berkelanjutan. Pupuk organik dapat dibuat dengan menggunakan alat dan bahan yang sederhana dan murah. Namun masyarakat desa umumnya masih belum familiar dan memahami mengenai teknik pembuatan pupuk organik. Berdasarkan hal tersebut, maka perlu dilakukan pelatihan pembuatan pupuk organik pada KWT di desa Ngestirahayu. Hasil akhir dari pelaksanaan kegiatan adalah terjadinya peningkatan pengetahuan masyarakat sebesar 91,6% mengenai pupuk, bahan, proses pembuatan pupuk organik. Manfaat kegiatan ini yaitu meningkatkan pengetahuan mitra mengenai penggunaan pupuk organik, mengetahui bahan yang digunakan dan tatacara pembuatan pupuk organik. Masyarakat perlu dilakukan pembiasaan untuk mulai memilah sampah berdasarkan jenisnya mulai dari skala rumah tangga, sehingga memudahkan untuk mengolah sampah menjadi pupuk kompos. Selain itu, perlu dilakukan produksi pupuk kompos dalam jumlah banyak untuk mencukupi kebutuhan pupuk desa, sebagai kegiatan lanjutan dari program ini. Compost Fertilizer Production Training in Ngestirahayu Village to Achieve Agricultural Independence Abstract The village of Ngestirahayu is one of the villages located in the Punggur District, where the majority of the residents work as farmers. Farmers have long faced issues, one of which is the limited availability and supply of subsidized fertilizers. Therefore, there is a need for an affordable and self-produced fertilizer alternative. In addition, organic fertilizers can improve soil quality and provide essential nutrients for plants in a more sustainable manner. Organic fertilizers can be made using simple and inexpensive tools and materials. However, the village community is generally not yet familiar with or knowledgeable about organic fertilizer production techniques. Based on this, it is necessary to conduct training on organic fertilizer production for the Women's Farming Group (KWT) in the village of Ngestirahayu. The final outcome of this activity was a 91.6% increase in community knowledge regarding fertilizers, materials, and the process of making organic fertilizers. The benefits of this activity include enhancing partners' knowledge of organic fertilizer use, understanding the materials used, and learning the process of making organic fertilizers. The community needs to develop a habit of sorting waste by type at the household level, making it easier to process waste into compost. Furthermore, large-scale compost production is needed to meet the village's fertilizer needs as a follow-up activity of this program.

Activity of Cellulolytic Bacteria Origin From Sediment of Mangrove Forest Ecotourism Pesawaran Lampung Chusniasih, Dewi; Handayani, Catie; Istiadi, Khaerunissa Anbar
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.371

Cellulolytic bacteria can hydrolyze substrates containing cellulose such as mangrove litter trapped in mangrove sediment by producing cellulase enzymes to break down cellulose into simpler compounds. The purpose of this study is to obtain bacterial isolates from mangrove sediment that have the ability to produce cellulase enzymes and to determine the activity of the cellulase enzymes they produce in degrading cellulose substrates. The methods used in this study include isolation of cellulolytic bacteria from mangrove sediments, qualitative enzyme activity test, standard curve of bacterial growth, bacterial growth curve for 24 hours, standard glucose curve, and quantitative enzyme activity test using a spectrophotometer. From the isolation results obtained 18 candidate cellulolytic bacteria with different macroscopic and microscopic characteristics. Based on the results of the qualitative enzyme test for 4 days on Zobell media added with 0.5% CMC, enzyme activity index was obtained from 5 isolates. The highest activity index of isolate M2 was on day 3 that was 1.96, isolate M3 had the highest activity index on day 4 that was 4.29, isolate M4 was 2.02 on day 4, isolate M6 was 4.39 on day 4, and isolate M13 was 3.87 on day 4. Furthermore, 3 potential iso-lates were selected based on the highest average index of enzyme activity for quantitative tests. The quantitative test results from the crude extract of the cellulase enzyme for 7 days on 0.5% CMC substrate showed that the highest hydrolysis activity occurred on day 5 by isolate M6, that was 0.151 U/mL. The other two cellulolytic isolates, isolate M3 and isolate M13, had the highest cellulose hydrolysis activity, 0.129 U/mL and 0.137 U/mL.

UTILIZATION OF PINEAPPLE PROCESSING LIQUID WASTE IN BIODEGRADATION OF DISPOSABLE FACE MASK BY BACTERIA FROM LAMPUNG BAY Deviany, Deviany; Zhofiroh, Nawrah Meisyah Muthi'ah; Febrina, Yane Fita; Yusuf, Reggina Aulia; Achmad, Feerzet; Yuniarti, Reni; Istiadi, Khaerunissa Anbar
Jurnal Bioteknologi dan Biosains Indonesia Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jbbi.11.2.8192

The designation of COVID-19 as a global pandemic led to an increased use of single-use face masks, which result in waste that is difficult to degrade and has the potential to release microplastic fibers into the environment. This study aims to examine the im-pact of adding pineapple peel liquid waste (LCN) as a growth medium for the biodeg-radation of single-use face masks by bacterial isolates obtained from the waters of the Lampung Bay. The study investigates how LCN affects the efficiency of mask degra-dation by microorganisms, as well as how the ratio of LCN mixed with other growth media, such as Nutrient Broth (NB), influences the degradation process. The biodeg-radation process was carried out using a biostimulation technique, where bacterial iso-lates were incubated in media containing LCN at a 1:1 ratio. The degradation process lasted for 15 days, with the results being analyzed using gravimetry and Fourier-Transform Infrared Spectroscopy (FTIR). Gravimetric results showed a greater weight reduction in treated masks compared to the control masks. FTIR analysis also indicat-ed changes in the intensity of functional groups in the degraded layers of the masks, as well as the emergence of C≡C functional groups in the second and third layers. This study demonstrates that the addition of LCN can accelerate the biodegradation of sin-gle-use face masks, offering a new approach for managing mask waste.

Growth of Female Mice (Mus musculus) Fed with Mung Beans (Vigna radiata) Based Feed Anggraini, Dina; Imaniar, Lisana Husna; Istiadi, Khaerunissa Anbar; Mulyana, Jeane Siswitasari; Anisa, Hida Arliani Nur
Konservasi Hayati Vol 21 No 1 (2025): APRIL
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v21i1.36980

Laboratory mice are widely used in biomedical research due to their genetic similarities to humans. The dietary needs of mice in Indonesia. However, they are frequently not satisfied by commercially available feed. This indicates the necessity for locally obtained, customized feed formulations, like those that include mung beans, which are high in vital nutrients and promote sustainable agriculture. This study aims to compare the effect of various mung bean-based feeds on the weight gain of female mice and their feed conversion value. Four feed formulations with varying concentrations of mung beans (85%, 75%, 65%, and 55%) were compared to commercial Broiler II feed, using 20 female mice over two months. The study involved daily feeding, weighing consumed feed, and mice’s body weight to determine the Feed Conversion Ratio (FCR), and the Kruskal-Wallis test for statistical analysis. The results show no statistical difference between treatments, which means all treatments are comparable to the control groups in promoting mice growth in all the parameters observed. Nevertheless, a certain trend was observed where the higher the mung bean content, the more similar the results to the control group. Thus, treatments MB85 containing 85% mung beans might have the greatest potential to promote mice growth.

Title

Found 15 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 15 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 15 Documents
Search