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I Putu Cahyadi Putra
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Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.2024.v16.i05.p01
Core Subject : Health, Science,
Immunology & microbiology Public Health Veterinary
FOCUS The journal focused on Veterinary Medicine, Animal Sciences, and Health Studies with various developments. SCOPE Zoonoses, Public Health, One Health, Epidemiology, Reproduction, Virology, Bacteriology, Parasitology, Mycology, Internal Medicine, Surgery, Orthopedics, Vaccines, Genetics, Molecular Biology, Physiology, Nutrition and Dietetics, Feed Science, Meat Science, Animal Welfare, Animal Behavior, Endocrinology and Metabolism, Animal Care, Veterinary Education, Livestock Management and Production, Poultry, Horse Science, Cattle Science, Small Ruminants, Pig Science, Aquaculture, Fish Diseases, Fisheries Science and Fish Nutrition, Zoo Animal Management, Zoo Animal Disease Studies, Wildlife Diseases, Conservation Science, and other related studies.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 394 Documents
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SURGICAL TREATMENT OF UMBILICAL HERNIA IN A LOCAL CAT Nethania Liady; I Gusti Agung Gde Putra Pemayun; Anak Agung Gde Jayawardhita
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p07

Abstract

Umbilical hernia is an anatomical abnormality in which the muscles around the umbilicus fail to close properly, causing part of the intestine or omentum to enter the hernia sac through the abdominal cavity. The purpose of this article is to present a case study of congenital umbilical hernia in cats, including methods of diagnosis, management, and treatment. A 3-month-old male local cat weighing 1.5 kg came with a lump in the abdominal area, accompanied by a ring and mass that could be pushed back in. According to the owner, the lump had been there since the cat was born and continued to grow over time. Based on the results of physical, clinical, and supporting examinations in the form of barium contrast X-rays, the cat was diagnosed with an umbilical hernia which is a congenital disease with a Fausta prognosis. Treatment is carried out by surgically repositioning the contents of the hernia in the form of intestines into the abdominal cavity and suturing. Postoperatively, the antibiotic cefotaxime injection was given, followed by oral antibiotics, nonsteroidal anti-inflammatory drug, and topical antibiotic cream. On the 8th day after surgery, the case cat was declared cured with a dry and fused surgical wound, normal appetite, normal defecation and urination. It can be concluded that the surgical procedure and subsequent treatment successfully cured the hernia. Cats with umbilical hernias should be treated as soon as possible to prevent the hernia opening from getting bigger and causing conditions that endanger the cat's health.
PREVALENCE OF BALANTIDIUM SP. INFECTION IN SWAMP BUFFALOES USED IN THE MAKEPUNG TRADITION IN JEMBRANA REGENCY, BALI Akilah Balkis; I Made Dwinata; Ida Bagus Made Oka
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p03

Abstract

Buffaloes play an essential role in society, both as a food source and in socio-cultural aspects. One tradition involving buffaloes is Makepung in Jembrana Regency, Bali, which requires the buffaloes to be in optimal health. Gastrointestinal parasitic infections, such as Balantidium sp., can affect buffalo health and pose zoonotic risks. This study aims to determine the prevalence of Balantidium sp. infection in Makepung buffaloes and analyze the factors associated with its prevalence, including age, management practices, and geographical region. This research employs an observational method with a cross-sectional study design. A total of 120 male Makepung buffaloes from three districts in Jembrana Regency Melaya, Negara, and Mendoyo were selected using purposive sampling. Fecal samples were collected and examined using a modified sedimentation method to detect Balantidium sp. presence. Data were analyzed descriptively, and relationships between variables were tested using the Chi-square test. The results showed that the prevalence of Balantidium sp. infection in Makepung buffaloes was 45.8%. Geographical region and feed source significantly influenced infection prevalence (P<0.05), whereas age and management factors, such as housing system, and cleanliness, did not show significant effects (P>0,05). Makepung buffalo farmers are advised to improve sanitation, improve drainage, separate the enclosures, and use antiprotozoal drugs under the supervision of a veterinarian to prevent Balantidium sp. infections and resistance.
MICROBIAL CONTAMINATION TEST NUMBER OF YEAST MOLD SIMPLICIA MARIGOLD FLOWERS AS STANDARD RAW MATERIAL FOR HERBAL MEDICINE Maulia Suciani; I Made Merdana; Samsuri; Nyoman Adi Suratma
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p15

Abstract

The marigold plant is widely cultivated for medicinal purposes, as an ornamental plant and natural food colouring. Marigold flowers (Tagetes erecta L.) contain secondary metabolites in the form of terpenoids, essential oils, phenols, flavonoids, carotenoids, and so on. Looking at its contents and pharmacological benefits, marigold flowers have the potential to be used as simplicia as a raw material for herbal medicine. This research aims to determine the value of Total Yeast and Mold Count (TYMC) test simplicia marigold flower (Tagetes erecta L.) as a raw material for medicinal preparations based on microbial contamination standards according to the General Standard Parameters of BPOM RI Regulation Number 32 of 2019 concerning Quality Requirements for Traditional Medicines. This research is a type of non-experimental observational research because there is no treatment of the research object with a research design in the form of descriptive-quantitative analysis. The level of microbial contamination is reviewed through the Yeast and Mold Count (TYMC) test. The research data obtained was quantitative data which was analyzed by counting the number of microbes that grew on Potato Dextrose Agar (PDA) media after incubation at the appropriate growth temperature. The Total Yeast and Mold Count is 1,4 x 102 CFU/gram. The research results showed that marigold flower simplicia met the microbial contamination requirements for yeast and Mold Count numbers (≤5x105). It can be concluded that the mimosa leaves simplicia sample can be processed into drug preparations. Further research is needed on pathogenic microbial contamination, as well as additional quality standardization tests on marigold (Tagetes erecta L.) simplicia.
MANAGEMENT OF CHRONIC MASTITIS DUE TO FUNGAL INFECTION IN MINIATURE PINSCHER DOGS WITH MASTECTOMY METHOD I Nyoman Perdana Adi Putra; I Gusti Agung Gde Putra Pemayun; Anak Agung Gde Jayawardhita
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p08

Abstract

Mammary tumors in dogs are often mistaken for mastitis due to their very similar clinical symptoms. The purpose of this article is to provide information on the surgical management of chronic mastitis cases caused by fungal infections through the method of mastectomy. A Miki dog developed a lump in the fourth left mammary gland. Physical examination showed that the mass had a solid consistency. Hematology tests revealed lymphocytosis, microcytic anemia, and thrombocytopenia. Radiographic examination showed a radiopaque mass in the mammary gland area. Based on the physical examination, clinical signs, hematology, and supported by radiographic findings, the dog was temporarily diagnosed with a mammary tumor, and mastectomy surgery was performed. Postoperative tumor tissue was sent for histopathological examination at the Denpasar Veterinary Center, and the result showed no metastatic tumor cells, but fungal hyphae, macrophages, and lymphocytes were found. The dog was finally diagnosed with chronic mastitis due to a fungal infection. After surgery, the dog was treated with cefotaxime sodium injection (20 mg/kg body weight twice a day) and meloxicam injection (0.2 mg/kg body weight once a day). On the 4th day post-surgery, the treatment continued with cefixime trihydrate (10 mg/kg body weight twice a day for 7 days orally). Additionally, FuFang Ejiao Jiang was given to increase platelet production, 1 ml mixed with food. The wound was cleaned with chlorhexidine, Kenalog ointment was applied, and Enbatic powder was also used. The dog was declared healed on the 9th postoperative day with a dried wound, healed skin, good appetite and drinking, and normal urination and defecation. Biopsy of abnormal tissue is highly recommended to identify the type of tumor for considering therapy plans. However, mastectomy surgery is also necessary in this case because the dog had chronic mastitis, which could contribute to further complications.
CASE STUDY OF NEWCASTLE DISEASE VELOGENIC STRAIN WITH MULTIORGAN LESSIONS IN VACCINATED BROILER IN SUSUT, BANGLI I Gde Andhika Putra Pratama; I Gusti Ngurah Kade Mahardika; Ida Bagus Oka Winaya; I Nengah Kerta Besung; Ida Bagus Made Oka
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p16

Abstract

Newcastle disease (ND) is a highly contagious viral disease of poultry with high mortality rates in velogenic strains. This paper present findings about velogenic strain of Newcastle disease in case study. The methods used in examination of animal case are observation, anatomical pathology examination, histopathology examination, TAB cultivation, serological tests, bacterial identification and parasite identification. In this case study, 28-day-old broiler chickens were obtained from a farmer in Demulih Village, Susut, Bangli. The clinical symptoms observed in the affected chickens included weakness, anorexia, difficulty breathing, greenish-white diarrhea, and torticollis. Pathological anatomical examination revealed congestion in the brain, hemorrhages in the trachea, lungs, heart, and intestines, as well as petechiae in the proventriculus. Histopathological examination showed inflammatory changes were dominated marked by mononuclear cell infiltration in the brain, trachea, lung, heart, proventriculus and intestine. Result of TAB cultivation continued with using HA test showed that virus can agglutination erythrocytes with titer 210 HA Unit. ND virus was confirmed by HI test. Based on this case study, it was concluded that the chickens were infected with ND.
SECONDARY ANTIBODY TITER RESPONSE IN PIGS FOLLOWING STREPTOCOCCUS SUIS VACCINATION WITH VARIOUS ADJUVANTS: DETECTION USING SUPERNATANT ANTIGEN-BASED ELISA Gede Gita Pratama; I Nengah Kerta Besung; Hapsari Mahatmi; I Gusti Ngurah Kade Mahardika; Ni Ketut Suwiti
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p09

Abstract

Streptococcus suis is a pathogenic bacterium that causes severe disease in pigs and poses a zoonotic risk to humans. Vaccination is one of the primary preventive strategies, and its effectiveness can be enhanced through the use of appropriate adjuvants. This study aimed to evaluate the secondary humoral immune response in pigs vaccinated with a locally derived S. suis vaccine formulated with two different adjuvants: Montanide ISA 201 VG and Montanide Gel-01. In addition, the study observed the dynamics of antibody titters over time post-vaccination. Twelve pigs were divided into three treatment groups: a control group (unvaccinated), a group vaccinated with Montanide ISA 201 VG, and a group vaccinated with Montanide Gel-01. Blood samples were collected weekly for five weeks following the booster vaccination, and antibody titers were measured using ELISA based on culture supernatant antigens. Data were analyzed using analysis of variance and linear regression. The results showed that both adjuvants significantly increased antibody titers compared to the control (p < 0.05), with Montanide Gel-01 producing the highest titer (0.718 ± 0.0397), followed by ISA 201 VG (0.703 ± 0.0320), and the control group (0.459 ± 0.0419). Furthermore, the timing of serum collection significantly influenced antibody titer levels (p < 0.005). This study concludes that the use of Montanide adjuvants, particularly Gel-01, is effective in enhancing the humoral immune response to S. suis vaccination. Further research is recommended to evaluate the effects of increasing antigen concentration for achieving a more robust immune response.
ANALYSIS OF MILK QUALITY AND TOTAL PLATE COUNT IN COW MILK TRADED IN YOGYAKARTA CITY AREA Nur Ika Prihanani; Muhammad Rosyid Ridlo; Febri Ariyanti; Nurulia Hidayah; Gihesa Lula Amalia; Ganita Gardin; Raisyha Kinanthi; Abiyyudha Hemiwarsanto; Dinda Ramadhan
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p09

Abstract

Ensuring food safety of animal origin is very important. Milk can contain microbial contamination as a result of contamination, poor management, and not implementing sanitary hygiene. Control of the quality of milk traded in the community needs to be carried out, so that safe, healthy, intact, and halal aspects are fulfilled. This study aims to determine the quality and contamination of microbes in cow's milk traded in the Yogyakarta City area. The sample consisted of 14 pasteurized milk and 6 samples of fresh milk. Milk quality testing includes specific gravity, fat content, and lean dry weight tests using Lactoscan® tools, as well as microbial contamination testing with the Total Plate Count (TPC) test. The data were analyzed descriptively. The results of the analysis of pasteurized milk showed an average specific gravity of 1.0183 g/ml, a fat content of 3.72%, and a dry weight without fat of 5.67%. The results of the analysis of fresh milk showed that the average specific gravity was 1.0187 g/ml, the fat content was 4.53%, and the dry weight without fat was 5.98%. The TPC test on pasteurized milk, 6 samples were not contaminated with bacteria and 8 samples showed an average TPC test result of 3.77x104 CFU/ml. The average TPC test on fresh milk was 0.742x106 CFU/ml. The quality of both milk meets the minimum requirements for specific gravity and fat content but has not met the minimum requirements for dry weight without fat, namely in pasteurized milk according to SNI 01-3951-1995 and fresh milk according to SNI 3141.1:2011. The quality of pasteurized milk and fresh milk meets the maximum requirements for microbial contamination according to SNI 7388:2009. Farmers are required to pay attention to the management aspect, especially feed management so that the specific gravity value and fat content can meet SNI standards.
DETERMINATION OF NON-SPECIFIC AND SPECIFIC QUALITY PARAMETERS OF MARIGOLD SIMPLICIA AS A RAW MATERIAL FOR MEDICINAL PREPARATIONS Martina; Samsuri; I Made Merdana; I Wayan Sudira; Putu Devi Jayanti
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p31

Abstract

Marigold flower (Tagetes erecta L.) is a type of ornamental plant from the Asteraceae family that has significant potential for cultivation due to its numerous uses. Besides being an ornamental flower, a natural dye, and an attractant for pest predators, marigold contains carotenoids, which can act as antioxidants, relieve mild fever and sore throat, serve as a natural moisturizer, and function as a natural mosquito repellent. Given its various beneficial components and properties, marigold has the potential to be used as a raw material for pharmaceutical preparations. This study aims to determine whether marigold flower simplicia meets the requirements as a raw material for pharmaceutical preparations based on non-specific and specific parameters according to established standards. The non-specific parameters include moisture content and ash content tests, while the specific parameters include organoleptic tests, water-soluble extract content, ethanol-soluble extract content, and yield percentage. This research is an observational non-experimental study with a descriptive-qualitative analysis design. Data collection was carried out by conducting non-specific and specific tests to obtain results for each parameter. The obtained results were as follows: moisture content 4.95%, ash content 4.8%, water-soluble extract content 37.34%, ethanol-soluble extract content 28%, and yield 13.48%. Based on these results, it can be concluded that the marigold flower simplicia is suitable and meets the requirements to be used as raw material for medicinal preparations, in accordance with the previously established parameters, namely the Materia Medica Indonesia Volume VI of 1995, the Ministry of Health of the Republic of Indonesia in 2017, and the Regulation of the Head of the Indonesian Food and Drug Authority (BPOM) Number 32 of 2019 concerning the quality requirements for traditional medicines. Further quality standardization tests should be conducted, such as microbial contamination, total aflatoxin, heavy metal contamination, weight uniformity, and alcohol content determination in marigold flower simplicia to establish additional quality standards across different parameters.
COINFECTION OF SEVERE TRICHURIOSIS AND COCCIDIOSIS IN A DUROC WEANER PIG Kezia Joana Limarta; I Ketut Berata; Hapsari Mahatmi; I Nyoman Mantik Astawa; I Putu Cahyadi Putra
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p10

Abstract

The presence of gastrointestinal parasites can inhibit the growth of weaning-phase pigs. In addition, gastrointestinal parasites can make pigs more susceptible to other pathogens and even cause death. This case report discusses the co-infection of severe trichuriosis and coccidiosis with secondary Escherichia coli infection in a Duroc weaner pig. Data were collected through anamnesis, epidemiological studies, clinical signs, anatomical pathology and histopathological examinations, and bacteriology and parasitology laboratory examinations. The case animal is a male Duroc pig, 2.5 months old, originating from Sulahan village, Susut sub-district, Bangli regency, Bali. The clinical signs observed were diarrhea with dark feces and decreased appetite. On anatomical pathology examination, 2329 adult Trichuris suis worms were found in the cecum and colon. Changes in the organs included wounds and hemorrhage in the cecum and colon, hemorrhage in the stomach and small intestine, and a singular white spot found on the uneven-colored liver. Histopathological examination showed enteritis hemorrhagis et necroticans, colitis necroticans verminosa, gastritis necroticans, and hepatitis necroticans. Bacteriological examination identified Escherichia coli in the intestine and liver specimens. Qualitative examination of feces revealed T. suis eggs and Eimeria spp. oocysts. According to McMaster's calculations, there were 36,200 eggs per gram (EPG) of T. suis and 15,800 oocysts per gram (OPG) of Eimeria spp. Based on all data, along with the results of laboratory examinations, it can be concluded that the pig was infected with severe trichuriosis and coccidiosis with secondary Escherichia coli infection. Pigs that are still alive and are confirmed to be infected with trichuriosis and coccidiosis should be treated.
THE EFFECT OF NATURAL GUARD IN DRINKING WATER ON NEWCASTLE DISEASE ANTIBODY TITER IN LAYING HENS Misfah Maulidiyah; Ida Bagus Komang Ardana; Ida Bagus Kade Suardana
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p17

Abstract

Natural Guard is a combination of pine oil, lavender oil and eucalyptus oil which can be used as an immunomodulator. The aim of this study was to determine the effect of Natural Guard administration through drinking water on the antibody titer against Newcastle Disease (ND) virus. This study used 24 Hy-line Brown laying hens randomly assigned to four treatment groups. Natural Guard was mixed into the drinking water at different concentrations: 0 ml/L (control), 0.1 ml/L (P1), 0.2 ml/L (P2), and 0.3 ml/L (P3), and administered for 30 days. ND antibody titers were measured using the Hemagglutination Inhibition (HI) test. The data obtained were analyzed using analysis of variance. The results showed that administration of Natural Guard at doses of 0.1 ml/L, 0.2 ml/L, and 0.3 ml/L did not significantly (P > 0.05) increase ND virus antibody titers in laying hens.

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