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I Putu Cahyadi Putra
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Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.2024.v16.i05.p01
Core Subject : Health, Science,
Immunology & microbiology Public Health Veterinary
FOCUS The journal focused on Veterinary Medicine, Animal Sciences, and Health Studies with various developments. SCOPE Zoonoses, Public Health, One Health, Epidemiology, Reproduction, Virology, Bacteriology, Parasitology, Mycology, Internal Medicine, Surgery, Orthopedics, Vaccines, Genetics, Molecular Biology, Physiology, Nutrition and Dietetics, Feed Science, Meat Science, Animal Welfare, Animal Behavior, Endocrinology and Metabolism, Animal Care, Veterinary Education, Livestock Management and Production, Poultry, Horse Science, Cattle Science, Small Ruminants, Pig Science, Aquaculture, Fish Diseases, Fisheries Science and Fish Nutrition, Zoo Animal Management, Zoo Animal Disease Studies, Wildlife Diseases, Conservation Science, and other related studies.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 394 Documents
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MICROBIAL CONTAMINATION TEST OF MIMOSA LEAVES SIMPLICIA Kezia Joana Limarta; Luh Made Sudimartini; Anak Dharmayudha
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.59

Abstract

The Mimosa plant (Mimosa pudica L.) is a wild plant that can be easily found in Indonesia and all of its parts have been used in traditional medicine, especially the leaves. Seeing its pharmacological content and benefits, the leaves of the Mimosa plant have the potential to be used as a simplicia as raw material for herbal medicine. This study aims to determine microbial contamination of mimosa leaves simplicia according to the General Standard Parameters of Medicinal Plant Extracts of the Ministry of Health of Republic of Indonesia in 2000 and Regulation of the Head of Food and Drug Monitoring Agency of Republic of Indonesia Number 32 Year 2019 concerning Quality Requirements for Traditional Medicines. This research is a non-experimental observational research with a comparative descriptive analysis design. The microbial contamination level was assessed by Total Plate Count (TPC) test and Total Yeast and Mold Count (TYMC) test. The research data obtained is quantitative data which was analysed by counting the number of microbes that grew on Plate Count Agar (PCA) media and Potato Dextrose Agar (PDA) media after being incubated at the appropriate growth temperature. The Total Plate Number of the Mimosa leaves sample is 3,9x104 CFU/gram and the Total Yeast and Mold Count is 2,5x103 CFU/gram. The results showed that the mimosa leaf simplicia met the microbial contamination requirements for Total Plate Number (£5x107) and Total Yeast and Mold Count (£5x105). It can be concluded that the mimosa leaves simplicia sample can be processed into drug preparations. Further research on pathogen Escherichia coli, Enterobacteriaceae, Clostridia, Salmonella, and Shigella microbial contamination and other quality standardization tests are needed.
PREVALENCE OF ECTOPARASITE TICK AND MITE INFESTATION ON DOMESTIC CHICKEN IN BALI Hesty Kartika Tandisalla; Ida Bagus Made Oka; I Made Dwinata
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.60

Abstract

The raising of free-range chickens is generally intended as laying hens, broilers, and pets by the people of Indonesia. One of the common diseases affecting free-range chickens is the infestation of ectoparasites ticks and mites that can interfere with the health of chickens and even cause economic losses on a farm. The aim of this study was to determine the prevalence of ectoparasite infestation of ticks and mites and to identify the types of ticks and mites that infest free-range chickens in Bali. The objects in this study were tick and mite ectoparasites that infested 5-6 month-old of 60 free-range chickens. Ticks ectoparasites were taken directly one by one using tweezers and for non-scabies mites were observed using a magnifying glass and taken using cotton wool soaked in 70% alcohol. Each ectoparasite taken from one individual chicken was put into an eppendorf tube filled with 70% alcohol. Identification was done by examining the preparations using a light microscope based on the CDC identification key (1966). The results of this study showed that the prevalence of ectoparasites in free-range chickens in Bali was 78% with a prevalence of ticks at 32% and a prevalence of mites at 78%. The types of ticks found were Haemaphysalis spp. and Megninia ginglymura mites. Further research is needed to identify the types of ectoparasites ticks and mites that infest chickens in Indonesia.
QUALITY OF BALINESE BEEF PRODUCED BY PESANGGARAN SLAUGHTERHOUSE WITH ABIANSEMAL VILLAGE ABATTOIRS Anak Agung Sagung Sandatsari Dewi; I Ketut Suada; I MAde Sukada
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.61

Abstract

The increasing demand for beef causes the intensity of cattle slaughter to increase. This makes the existence of slaughterhouses (RPH) and abattoirs (TPH) indispensable in supporting the availability of meat in an area. The purpose of this study was to determine the quality of Balinese beef in terms of subjective and objective tests and to determine the difference in the quality of Balinese beef produced by Pesanggaran slaughterhouses and Abiansemal Village of abattoirs. The samples used in this study were Balinese beef slaughtered at Pesanggaran slaughterhouses and Abiansemal Village abattoirs, at the silver side (M. biceps femoris) as much as 125 grams/head of 16 meat samples. Each sample was subjectively (color test, odor test, consistency test, and texture test) and objectively (pH test, water binding capacity test, and moisture content test) tested for physical quality. Subjective data was analyzed by Mann Whitney test and objective data was tested by T test (Independent). The results of the subjective data research with the Mann Whitney test obtained the results of the color test of Balinese beef produced by RPH Pesanggaran and TPH Abiansemal Village respectively 6.03 and 8.77, the odor test both had a score of 2, the consistency test had a score of 2.6 and 1.8, the texture test had a score of 2.43 and 1.38. While the T Test (Independent) test for objective data obtained the pH test results of 5.48 and 5.6, respectively, the water binding capacity test was 73.29% and 76.07%, and the water content test was 66.42% and 69.36%. It can be concluded that the quality of Balinese beef from Pesanggaran slaughterhouses and Abiansemal Village abattoirs is in good condition and suitable for consumption. In order to obtain ASUH meat products, it is recommended to carry out guidance on abattoirs in accordance with the standards for slaughtering animals at slaughterhouses.
EHRLICHIOSIS AND DEMODICOSIS IN BALI LOCAL DOG Muhammad Farhan Al Ma’arif; Putu Devi Jayanti; I Gede Soma
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.62

Abstract

Ehrlichiosis is a zoonotic disease caused by infection with intracellular bacteria Ehrlichia sp. which can be transmitted by ticks. Demodicosis is a parasitic disease caused by the mite Demodex sp. which has a predilection for the dermis, hair follicles, and sebaceous glands. A male local breed dog aged approximately 2 years with a body weight of 8 kg came with complaints of hair loss and high intensity itching with a scale of 8/10. The results of clinical examination showed alopecia, scale, and erythema almost all over the body accompanied by tick infestations found on the back, anemic mucosa, left prescapularis lymph nodes and left popliteus experienced swelling. Hematological examination showed macrocytic hypochromic anemia, leukocytosis, lymphocytosis, granulocytosis, and thrombocytopenia. Blood smear and serology examination showed Ehrlichia sp.. infection while deep skin scrapping examination showed Demodex sp. infection. Based on the anamnesis, physical examination and laboratory examination, the case of the dog suffering from ehrlichiosis accompanied by demodecosis. The therapy given was in the form of doxycycline, ivermectin, diphenydramine, vitamin B complex, and bath therapy with sulfur soap. Observation of therapy on the 14th day provided clinical improvement in terms of reducing itching intensity, Demodex sp. infection intensity, and hematological parameters. On the 28th day of the last treatment, the dog's body condition was getting better, new hair started to grow and the body condition score increased. Dogs should be given food rich in nutrients such as liver, meat and egg yolks, as well as providing nutritional supplements to speed up the dog's recovery.
COMMUNITY KNOWLEDGE, ATTITUDE, AND BEHAVIOR TOWARDS RABIES IN BANJAR BATUSARI, SANGEH VILLAGE, ABIANSEMAL DISTRICT, BADUNG REGENCY I Gusti Ngurah Putu Krisnu Mahaputra; I Wayan Masa Tenaya; I Made Sukada
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.63

Abstract

Rabies is a zoonotic disease caused by the genus Lyssavirus of the Rhabdoviridae family which is very fatal. In Indonesia, rabies has infected 26 of the 34 existing provinces. Bali has tested positive for rabies since 2008. The government and the people of Bali have made efforts to control rabies by carrying out mass rabies vaccinations for dogs, eliminating dogs that are not owned, and conducting education about the dangers of rabies to the public. This study aims to determine the knowledge, attitudes and behaviour of the community towards rabies in Banjar Batusari, Sangeh Village, Abiansemal District, Badung Regency, Bali. Data collection in this study was carried out directly by going to the field to conduct surveys and interviews with the community. The number of heads of families in Banjar Batusari is 153 families. Data obtained from the results of interviews regarding aspects of knowledge, aspects of attitudes, and aspects of the behaviour of the dog keeping community were tabulated using Ms. Excel, the data were analysed descriptively and qualitatively and displayed in the form of tables or graphs. The results showed that 100% of the knowledge of the people in Banjar Batusari had a high level of knowledge. The attitude of the people of Banjar Batusari can be categorized as a 100% positive attitude towards rabies. For the level of behaviour of the Banjar Batusari community, it is classified as good behaviour at 68.8%. Thus, the knowledge, attitude and behaviour of the community is expected to control rabies in the research location so that rabies cases are very small and make it easier to carry out rabies prevention and control there.
HEART HISTOPATHOLOGY CHANGES OF WHITEWISTAR RATS SATISFIED WITH CHRONIC APPICAL PERIODONTITIS POST ADMINISTRATION OF GREEN MENIRAN LEAF ETHANOL EXTRACT IN CALCIUM HYDROXIDE AND 2% CHLORHEXIDIN DIGLUCONATE AS ROOT CANAL MEDICAMENT PASTE Komang Hendry Wibawa Pramartha; Luh Made Sudimartini; Anak Dharmayudha; Ni Kadek Eka Widiadnyani
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.64

Abstract

Periodontitis is a disease that affects cats and dogs, which the herbal medicine that is used to treat periodontitis, namely meniran. Meniran is used as pain reliever and has antioxidant, anti-inflammatory properties, and antibacterial. The aim of this research was establishing the allotmen of ethanol extract of green meniran leaves on the histopathological features of the heart of Wistar rats with chronic apical periodontitis. This research of laboratory experiment was using the control of group desgn posttest randomly that conducted in several stages. Four groups was made for the test subjects that is consisting of 12 rats each, and each group was tested and divided into 3 groups. Each rat was dissected from one heart preparation in five microscopic fields of view. The analyzed data was the rat heart that is examined histopathologically to see if there is necrosis and congestion. In addition, the analysis was actualized by concerning the effect of the ethanol extract in green meniran leaves that was gived to histopathology of the heart utilizing the non-parametric statistical test, namely Kruskal-Wallis. The Kruskal-Wallis analysis resulted that treatment using ethanol extract of green meniran leaves had an effect on relieving necrosis (p<0.05) and congestion (p<0.05) in the heart organ, which means better than control group. Based on the result, the conclusion was obtained that the distribution of ethanol extract in green meniran can improve the histopathology of the heart of Wistar rats towards normal as seen from a decrease in congestive lesions and necrosis, especially in K3 given calcium hydroxide, 2% chlorhexidine digluconate and 10% of ethanol extract of green meniran. The future research need to inspect the impact of giving a combination of calcium hydroxide, 2% chlorhexidine digiluconate and 10% of ethanol extract green meniran in the long term.
THE EFFECT OF GIVING NATURAL GUARD ESSENTIAL OIL IN DRINKING WATER ON BLOOD UREA NITROGEN LEVELS, CREATININE, AND AMINOTRANSFERASE IN BROILERS Muhamad Abdul Shidiq; Ida Bagus Komang Ardana; I Ketut Sumadi; I Made Merdana; Luh Made Sudimartini; Yousef Haig Setrak Babikian; Haig Yousef Babikian; Rubiyanto Widodo Haliman; Kristina; Theng In Yen; Hendi Yanto Efendy
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.66

Abstract

To increase growth and feed efficiency, we can use feed additives through feed or drinking water, one of which is the use of essential oils. NATURALGUARD is  a mixture of natural oils, namely pine oil (Pinus sp.), lavender (Lavandula sp.), and eucalyptus oil (Eucalyptus sp.) which contains active compounds as immunomodulators, antibacterials, antioxidants, anti-inflammatory, antifungal and antiviral. This study aims to determine the level of Blood Urea Nitrogen (BUN), Creatinine, Aspartate Aminotransferase (AST), and Alanine Aminotransferase (ALT) in broilers after being given NATURALGUARD via drinking water. This study used a completely randomized design with a total sample of 24 broiler CP-707 strain, with four treatments and six replications. The treatment applied was P0 as a control without giving NATURALGUARD, P1 P2, and P3 were given NATURALGUARD at doses of 1, 2, and 4 g/L respectively, adlibitum for 30 days. At the end of the study, 3 mL of blood sample was taken via the pectoral vein aseptically, and then a blood serum examination was carried out. The research results showed that the highest mean BUN level was at P0 (8.9 mg/dL) and the lowest was at P1 (5.2 mg/dL), the highest creatinine was at P3 (1.08 mg/dL) and the lowest was at P0 and P1 (1, 0 mg/dL), AST was highest at P0 (273 µ/L) and lowest at P2 (220 µ/L), and ALT was highest at P0 (7.5 µ/L) and lowest at P3 (5.48 µ/ L). Statistical results showed no significant differences in BUN, creatinine, AST, and ALT levels in all treatment groups. It was concluded that NATURALGUARD as feed additive at doses of 1, 2, and 4 g/L via drinking water is safe to be used in broilers.
MUSCA SPP. FLIES INFESTATION ON A PIG FARM IN THE CITY OF DENPASAR Maria Fatima Suzana das Dores Sanches; I Made Dwinata; Ida Bagus Made Oka
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.67

Abstract

The role of flies in spreading disease is as a mechanical vector and a biological vector. As a mechanical vector flies carry pathogenic agents through their limbs. The fly's body has many hairs, especially on its legs. The hairs on the legs contain a kind of adhesive liquid so that small objects stick easily. Diseases transmitted by flies depend on the species. This study aims to determine the fluctuations and differences in the number of Musca spp. in a different environment on a pig farm in Denpasar City. This type of research is an observational study. The sample for this study were Musca sp. flies in two pig farms in Denpasar City. Samples were taken every day for 4 weeks at 09.00 – 14.00. Data analysis used the Wilcoxon test. The results showed that the Musca spp. found in pig farms in Ubung Kaja Village, North Denpasar, as many as 358 pigs and in Sidakarya Village, South Denpasar, as many as 981 pigs. The population of flies on pig farms in Ubung Kaja Village, North Denpasar and Sidakarya Village, South Denpasar, during 4 weeks of observation, fluctuated with the highest decrease in the 4th week and while the increase occurred in the 2nd week in North Denpasar, while in South Denpasar there was an increase in the 2nd week. up to 3. Based on the results of the study, it can be concluded that the population of flies on pig farms in Ubung Kaja Village, North Denpasar and Sidakarya Village, South Denpasar, during 4 weeks of observation, fluctuated with an increase occurring in the 3rd week in North Denpasar, while in South Denpasar there was an increase in the 2nd to 2nd week. 3. There is no difference in the number of Musca spp. in a different environment on a pig farm in Denpasar City. From these results, it can be suggested that pig farms should further improve environmental sanitation in their pens and surroundings by disposing of waste in properly managed bins and implementing good biosecurity. The research can be continued by increasing the number of places studied at different seasons so that the fluctuations of flies in the rainy and dry seasons can be determined.
HAEMATOLOGICAL PROFILE OF LAYING HENS AFTER NEWCASTLE DISEASE VACCINATION Anak Agung Sagung Kendran; Gusti Ayu Yuniati Kencana
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i3.p18

Abstract

Newcastle disease (ND) is a viral disease that can cause high mortality in chickens. Hundreds of chickens died due to ND disease. Morbidity and mortality due to ND disease is very large. Viral diseases cannot be treated with antibiotics. Therefore prevention can only be done by vaccination. However, vaccination failure often occurs, it has been proven that there are still many reports of ND cases. Thus, to find out early before sick chickens are attacked by ND, this research aims to determine the hematological profile including: RBC, Hb, HCT, MCH, MCHC, MCH, PLT, WBC, Eosinophils, Monocytes, Neutrophils and Lymphocytes. The research method was a field survey with purposive sampling with blood sampling as a repeat period. The research sample consisted of 40 laying hens, 30 of which were vaccinated with the active ND vaccine of the Lasota strain and 10 without vaccination. Blood samples were taken with anticoagulation four times, namely once pre-vaccination and three times post-vaccination. The data obtained were analyzed by means of variance followed by the Duncan test using SPSS version 25. The results of the study stated that RBC, Hb, HCT, MCV, Neutrophils and Lymphocytes were significantly different (P<0.05). Meanwhile MCV, MCHC, PLT, WBC, Eosinophils and Monocytes were not significantly different (P>0.05).
IDENTIFICATION AND PREVALENCE OF GASTROINTESTINAL PARASITIC WORMS IN KING COBRAS IN BALI Ayala Lubiana Naro; Nyoman Adi Suratma; Sri Kayati Widyastuti
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.69

Abstract

The king cobra (Ophiophagus hannah) has an important role in the ecosystem, this snake eats other snakes so it has a role to keep the number of snakes in balance. However, until now parasitic worm infections in wild king cobras in Bali have not been reported, so research is needed to determine the genus of parasitic worms that infect wild king cobras in Bali and their prevalence. This research was conducted on 15 wild king cobras by taking faecal samples which were examined using the floating method, and taking oral samples which were examined directly under a microscope. The test results showed that wild king cobras in Bali can be infected with nematodes of the genus Kalicephalus sp., Strongyloides sp., Capillaria sp., and Rhabdias sp. Any king cobra infected with worms can have a single or mixed infection. The results of the research shows that the type of worm that infects the king cobras in Bali are worms of the genus Kalicephalus sp. (100%), Strongyloides sp. (60%), Capillaria sp. (73.3%), and Rhabdias sp. (6.67%), each king cobra can be infected by 1-4 types of worms, and the oral test of the snake only found Kalicephalus sp. (53.3%). It can be concluded that the types of worms that infect king cobras in Bali are nematodes of the genus Kalicephalus sp., Strongyloides sp., Capillaria sp., and Rhabdias sp. The prevalence of worms that infect king cobras in Bali is Kalicephalus sp. (100%), Strongyloides sp. (60%), Capillaria sp. (73.3%), and Rhabdias sp. (6.67%). On oral examination, Kalicephalus sp. (53.3%). It is recommended for snake keepers or veterinarians who handle snakes to examine snakes for worm infections. In addition, it is necessary to carry out further research regarding the identification and prevalence of gastrointestinal worm parasites and other types of worm parasites.

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