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I Putu Cahyadi Putra
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Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.2024.v16.i05.p01
Core Subject : Health, Science,
Immunology & microbiology Public Health Veterinary
FOCUS The journal focused on Veterinary Medicine, Animal Sciences, and Health Studies with various developments. SCOPE Zoonoses, Public Health, One Health, Epidemiology, Reproduction, Virology, Bacteriology, Parasitology, Mycology, Internal Medicine, Surgery, Orthopedics, Vaccines, Genetics, Molecular Biology, Physiology, Nutrition and Dietetics, Feed Science, Meat Science, Animal Welfare, Animal Behavior, Endocrinology and Metabolism, Animal Care, Veterinary Education, Livestock Management and Production, Poultry, Horse Science, Cattle Science, Small Ruminants, Pig Science, Aquaculture, Fish Diseases, Fisheries Science and Fish Nutrition, Zoo Animal Management, Zoo Animal Disease Studies, Wildlife Diseases, Conservation Science, and other related studies.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 394 Documents
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COMPARISON OF MANUAL AND AUTOMATIC METHODS IN DETERMINATION OF HEMATOCRIT AND HEMOGLOBIN LEVELS IN GREEN SEA TURTLE Rindar Mentari Nusanti Putri; Ida Bagus Adnyana; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.70

Abstract

Sea turtles as protected animals in Indonesia need to recover and increase their population through comprehensive rehabilitation efforts. Examination of hematocrit and hemoglobin in green sea turtles (Chelonia mydas) at the rehabilitation center can function to determine the condition of green sea turtles because arriving turtles have different conditions. Dehydration and anemia in green sea turtles can occur due to transportation of turtles and unsuitable environmental conditions before the turtles were rescued. Examination of hematocrit and hemoglobin can be done manually or using a hematology analyzer. However, automated hematological analysis used for mammals generally not applicable for reptiles including green sea turtles due to the presence of nucleated erythrocytes. This study aims to compare the results of hematocrit and hemoglobin examination in green turtles using manual and automatic methods. This study used blood samples from 10 green turtles at the Turtle Conservation and Education Center (TCEC), Serangan Bali. Then a manual check was carried out at TCEC, Serangan, Bali and automatically using the Vet AutoRead IDEXX tool. Manual hematocrit examination uses the microhematocrit method and manual hemoglobin examination uses the Sahli method. The results were then analyzed using the Statistical Product and Service Solution (SPSS) application. Analysis of the significance of manual and automatic calculations of hematocrit and hemoglobin using paired sample t-test. The results of hematocrit (p=0.972) and hemoglobin (p=0.841) showed no significant difference (p>0.05). It can be concluded that the examination of hematocrit and hemoglobin in green turtles is not different so that it can be done manually and automatically.
THE AKUT TOXICITY TESTING LETHAL DOSE ORAL GREEN MENIRAN LEAF EXTRACT BY USING MICE Ni Kadek Eka Widiadnyani; Luh Made Sudimartini
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.71

Abstract

The use of a dose limit of green meniran leaf extract (Phylanthus niruri Linn) must be known for its safety which is supported by research results. The aims of this research to determine the acute lethal dose (LD50) toxicity and to examine the macropathological changes in the vital organs of mice. A total of 30 male mice and 30 female mice were divided into five groups and six replicates. Experimental mice in the control group (K) were given 0.9% NaCl, while those given meniran extract at doses of 50, 300, 2000 and 5000mg/KgBW were grouped as treatments. The preparation was given orally once and then the observations were made in the first 30 minutes after giving the preparation and periodically every 4 hours for the first 24 hours and once a day then the mice were observed for clinical symptoms, mortality and body weight. At the end of the study (day 14), the experimental mice were sacrificed for macropathological evaluation. giving meniran extract orally up to a dose of 5000 for 14 days did not cause death or showed toxic symptoms and normal growth. On macropathological examination of size, color and consistency, all organs were in normal condition. Green meniran leaf extract preparations > 5000 mg/kgBW are practically non-toxic and didn’t cause organ abnormalities. It is necessary to do research on toxicity, biocompatibility, effects, and potential for subchronic toxicity to changes in histopathological structure with a longer study duration, higher doses and more varied dosage ranges.
LEVEL OF BIOSECURITY IMPLEMENTATION IN CLOSED HOUSE BROILER CHICKEN FARMS IN SUSUT DISTRICT, BANGLI REGENCY, BALI Luh Putu Manik Samitha Utami; I Ketut Suada; Nyoman Adi Suratma
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i03.p02

Abstract

Biosecurity is a management step that must be taken by farmers to prevent disease germs from entering the farm and to prevent diseases on the farm from spreading to other farms. This research aims to determine the implementation and level of application of biosecurity components in closed house cages for broiler chicken breeders in Susut District, Bangli Regency, Bali. The method used is a census of respondents, namely all breeders. The data obtained from the interviews is tabulated and then the percentage of the amount of data that is in accordance with biosecurity implementation practices is calculated. The results show that overall farmers have implemented biosecurity components with each biosecurity component, namely the sanitation component showing the Very Good category (85%), the traffic control component showing the Poor category (38.81%) and the sanitation component showing the Very Good category. (97.7%). From the results obtained, chicken farmers should further improve their biosecurity implementation system, especially for guests/farm workers, because a very big factor in the spread of disease is the traffic component, including people, equipment and vehicles.
IDENTIFICATION OF BLOOD AND GASTROINTESTINAL PARASITES IN K-9 DOGS AT THE REPUBLIC OF INDONESIA STATE POLICE OF MALANG CITY RESORT Adithya Fauzan; Nyoman Adi Suratma; Sri Kayati Widyastuti
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.74

Abstract

K9 dogs are one of the animals of the Indonesian National Police where they have a fairly extreme and heavy work environment in assisting tasks such as finding explosives, drug tracking operations, security operations and searching for victims of natural disasters such as landslides to earthquakes. This study was conducted to determine the incidence of infection and the types of blood and digestive tract parasites that infect K9 dogs in the Indonesian National Police Malang City Resort. The blood examination method used is by observing blood reviews with Diff-quick staining and fecal examination using the flotation concentration method which is then observed with a microscope. The results of the incidence of parasitic infections in K9 dogs of the Indonesian National Police Malang City Resort were 100%, namely from 10 dog samples, 50% were infected with blood parasites and 60% were infected with digestive tract parasites. In the blood test examination only found Babesia sp. while in the feces examination found Ancylostoma sp., Strongyloides sp. and Trichuris sp. It is recommended to take countermeasures in the form of routine anti-parasitic administration.
HISTOPATOLOGICAL CHANGES OF WHITE RATS' SKIN CAUSED BY APPLICATION OF MIMOSIN FROM SIMPLISIA OF THE LAMTORO LEAF Dwi Muslifah Nur Hijayani; I Ketut Berata; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.75

Abstract

Mimosine is one of the substances contained in lamtoro (Leucaena leucocephala) leaves. The toxic effect of mimosine on livestock is highly dependent on the concentration of mimosine in feed ingredients and the length of time livestock consume feed high in mimosine. This study aims to determine the histopathology of white rat skin exposed to mimosine compounds with different doses. This study used male white rats with Wistar strain, 2 months old and 300-350 g body weight. The 20 rats used were divided into four treatment groups, namely P0 (negative control, P1 (positive control, given standard mimosine 5 mg/head/day), P2 (given lamtoro leaf simplisia at a dose of 50 mg/head/day orally), P3 (given lamtoro leaf simplisia at a dose of 150 mg/head/day). On the 15th day of necropsy, the skin organs were taken and fixed using 10% NBF. After the skin organs were fixed, histopathology preparations were made using HE staining. Histopathologic examination was performed including three lesion variables: hair follicle necrosis, congestion, and inflammation. The severity of the lesions was scored as 0, 1, 2, and 3 for normal, mild, moderate and severe lesions, respectively. Data were then analyzed using Kruskal-Wallis and Mann-Whitney non-parametric tests. The results showed that exposure to mimosine from lamtoro leaf simplisia did not cause hair loss in experimental animals as it does in cattle. However, exposure to mimosine in P1 (5 mg/head/day) and P3 (150 mg/head/day) caused histopathological skin lesions in the form of congestion, necrosis, and mild inflammation. The results showed that exposure to mimosine from lamtoro leaf simplisia did not cause hair loss in experimental animals as it does in cattle. However, exposure to mimosine in P1 (5 mg/head/day) and P3 (150 mg/head/day) caused histopathological skin lesions in the form of congestion, necrosis, and mild inflammation. The conclusion of the study is that mimosine compounds from lamtoro leaf simplisia cause histopathological changes in white rat skin tissue, especially congestion lesions. However, there was no difference in the effect of mimosine administration from lamtoro leaf simplisia between a dose of 50 mg/head/day and a dose of 150 mg/head/day on the histopathology of rat skin tissue. Further research needs to be done on the effect of exposure to mimosine from lamtoro leaf simplisia with higher concentrations.
DETECTION AND IDENTIFICATION OF NEMATODE WORM EGGS IN LAYING HENS AT HASNA FARM, WAJO REGENCY AND ANDI MUKTI FARM, PINRANG REGENCY, SOUTH SULAWESI Anggi Aprianti; Lilis Juniarti; Andi Magfira Satya Apada; Muh. Danawir Alwi; Zainal Abidin Kholilullah; Zulfikri Mustakdir; Fedri Rell
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.76

Abstract

Laying chickens are a type of poultry that has the potential to produce quite large eggs in Indonesia. One of the pathogens that often attacks laying hens is nematode worms which can cause economic losses for farmers. This research aims to detect and identify the presence of nematode worm eggs that infect laying hens on the Hasna farm, Wajo Regency and the Andi Mukti farm, Pinrang Regency. The research was carried out at the Maros Veterinary Center Laboratory and the Integrated Veterinary Laboratory at Hasanuddin University. The samples used in this research were 48 feces taken from each farm from laying hens. Samples were examined using three test methods, namely native test, floating method and sedimentation method. The results obtained were that on Hasna's farm there were 7 individuals that were detected positive for nematodes, of which there were 3 types of worm eggs, namely Ascaridia galli, Heterakis gallinarum and Strongyloides sp and 12 animals on the Andi Mukti farm were detected positive for nematodes, where there were 2 types of worm eggs, namely Ascaridia galli and Heterakis gallinarum. Based on this research, there was nematode worm infection on Hasna and Andi Mukti's farms.
COCCIDIOSIS IN BROILER CHICKEN AGED 26 DAYS FROM A FARM IN BANJARANGKAN, KLUNGKUNG, BALI Luh Putu Syamadina Pramesya Nareswari; Nyoman Adi Suratma; I Ketut Berata; Putu Henrywaesa Sudipa; Ida Bagus Kade Suardana
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i03.p04

Abstract

The object of this case study is a broiler chicken from a farm located in Banjarangkan District, Klungkung Regency, Bali. This broiler chicken is 26 days old which was reported to have had diarrhea mixed with blood for 5 days. This chicken also experience weakness, pale wattles, and separate itself in the corner of the shed. This case study aims to discuss the anatomical pathology, histopathology, and laboratory examination results to obtain a definitive diagnosis of the disease that attacked this chicken. Data collection in the form of indications, anamnesis, epidemiology, with parasite examination were carried out for further descriptive qualitative analysis. Necropsy after the animal died showed bleeding, and necrosis of the cecum accompanied by enlargement from cecum to colon. Petechiae bleeding were also observed along the small intestine. Histopathological observations showed necrosis in the mucosa of the cecum and small intestine accompanied by bleeding, inflammatory cell infiltration, and clusters of Eimeria spp. with different life stages. Changes also occur in the colon where necrosis of mucosa and proliferation of Lieberkhun crypts were observed. Schizonts of Eimeria spp. were found in qualitative fecal examinations. Based on all data and laboratory examination results of this chicken, it was concluded that the chicken was infected with coccidiosis.
COMMUNITY KNOWLEDGE AND ATTITUDE TO RABIESIN IN THE BANJAR PEKANDELAN, BEDULU VILLAGE, BLAHBATUH DISTRICT, GIANYAR REGENCY I Gede Arya Bayu Mahendra; I Wayan Masa Tenaya; Romy Muhammad Dary Mufa
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.85

Abstract

Rabies is known as Lyssa, hygrophobia or also mad dog disease which is caused by a virus from the genus Lyssavirus, family Rhabdoviridae which is acute and attacks the central nervous system. Rabies eradication does not only depend on the problem of dogs or other rabies-transmitting animals, but also involves human problems. This study aims to determine the knowledge and attitudes of the community about Rabies Disease in Banjar Pekandelan, Bedulu Village, Blahbatuh District, Gianyar Regency, Bali. There are 64 household heads in Banjar Pekandelan and 13 families who keep dogs. The design of this research is observational. The number of respondents in this study were all families who kept dogs in Banjar Pekandelan. Research data collection was carried out by interviewing the dog owner's family using a questionnaire. The data obtained from interviews regarding the knowledge and attitude aspects of the dog keeping community were tabulated using Ms. Excel, data were analyzed descriptively qualitatively and displayed in table form. From the research results, it was found that the community already ewell understood about rabies. While the attitude of the community is worth, which means that the community has a positive attitude in dealing with rabies in dogs. It can be concluded that the knowledge and attitude of the community in Banjar Pekandelan, Bedulu Village towards rabies is high and positive in dealing with rabies. So that it makes it easier to carry out the prevention and control of rabies there
OPTIMISATION OF PRIMER CONCENTRATION AND ANNEALING TEMPERATURE IN PCR TEST METHOD FOR AFRICAN SWINE FEVER VIRUS DETECTION Luh Dewi Anggreni; Ni Made Ritha Krisna Dewi; I Gusti Ngurah Kade Mahardika; I Gusti Ngurah Narendra Putra
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.88

Abstract

African Swine Fever disease causes high fatality number in domestic pigs. The most sensitive and specific laboratory diagnotic is PCR. The successful of PCR is determined by DNA quality, Annealing temperature, MGCl2 concentration, polymerase enzymes, primer concentration, and PCR cycles. The Annealing process requires in the right temperature. The difference annealing temperature causes amplification process failure. The specificity and sensitivity of PCR products are determined by the optimal annealing temperature. PCR optimization, variations of time, temperature, and primer concentration can be adjusted. This study was conducted by PCR testing to detect DNA ASF virus using primer concentration and the most optimum Annealing temperature. Identification of primer concentrations and annealing temperature begins to make PCR components volume 10 µl consist of 5µL PCR Master Mix, a pair of ASF primer 0.5 µl, 1µL DNA templates, and 3µL ddH2O. The PCR component is processed in a thermocycler with a temperature condition of 95°C pre-denaturation for 5 minutes, and denaturation at 94°C for 45 seconds. The gradient annealing temperature starts at 50°C, 52°C, 55°C, 59°Cm and 65°C for 1 minute. Temperature 72 ° C for synthesis 1 minute. repetition of the cycle is 34 times. Final synthesis for 72°C for 5 minutes, the storage temperature is 22°C. The result of optimization at the primer concentration of 10 µm obtained a cleared band according to the target compared to the primary concentration of 5 µm. The best annealing temperature in this research is 55 °C. If the Annealing temperature is given lower, causing primer attachment to the DNA template is not specific so non-specific PCR products are formed many bands. Conversely, if the annealing temperature is higher, causing primary attachment to the template DNA will be released so that the band that appears is very thin PCR product is not formed does not appear.
URINE ANALYSIS OF ETAWA BREED GOATS IN SUKAWATI DISTRICT, GIANYAR REGENCY BALI Gusti Putu Arni Safitri; Anak Agung Sagung Kendran; Luh Gde Sri Surya Heryani; Ida Bagus Komang Ardana; I Nyoman Suarsana; Sri Kayati Widyastuti; Romy Muhammad Dary Mufa
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i03.p07

Abstract

Etawa crossbreed goats (Capra aegagrus hircus) are livestock that are starting to be developed among breeders. In goat farming there are health problems that arise both clinically and subclinically, including disorders of the urinary tract. This study aims to determine the clinical condition of Etawa crossbreed goats (PE) in Sukawati District, Gianyar Regency by using urine examination. This study used 30 urine samples from Etawa crossbreed goats at the Raka Etawa Farm. A total of 10-30 ml of urine is taken using the mid-stream method and collected in a urine container which is then tested using a dipstick pad. This research is an observational study with a cross sectional study design using a purposive sampling method. Based on the results of the examination, data was obtained in the form of specific gravity (BJ) with a range of 1,005-1,010 and an average of 1.00567; pH in the range 8-9 and an average of 8.867; a total of 18 samples were found to contain proteins in the range 15-30 and an average of 15.83; and negative results were found in the parameters glucose, negative bilirubin, ketones, erythrocytes, urobilinogen, nitrites and leukocytes. These results indicate that the clinical condition of Etawa crossbreed goats (PE) is healthy (not sick) through urine examination in the form of physical examination and dipstick. It is necessary to carry out additional examinations in the form of examination of urine sidemen and bacterial culture, as well as measuring the specific gravity (BJ) using a refractometer to obtain a more accurate value.

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