African Journal of Clinical Medicine and Pharmacy Research
African Journal of Clinical Medicine and Pharmacy Research aims to publish rigorous, peer-reviewed scholarship that advances clinical medicine, pharmacy, and health-related sciences through scientifically sound, ethically grounded, and practically relevant research. • Clinical Advancement: disseminate high-quality studies that strengthen diagnosis, treatment, patient care, and evidence-based clinical practice. • Pharmacy and Therapeutics: promote research on drug development, pharmacology, pharmaceutical practice, therapeutics, and medication-related outcomes. • Health-Science Integration: encourage interdisciplinary links among medicine, pharmacy, pathology, microbiology, physiology, public health, and allied biomedical sciences. • Practice and Impact: support studies with meaningful implications for clinical decision-making, pharmaceutical care, healthcare systems, and patient safety. Submissions should clearly define the medical or pharmaceutical problem, report methods transparently, present defensible evidence, and articulate a meaningful contribution to clinical or pharmacy research. Scope AJCMPR welcomes original research papers and related scholarly contributions in clinical medicine, pharmacy, and associated health sciences, especially studies with clear scientific rigor, biomedical relevance, and practical healthcare implications. • Clinical Medicine: internal medicine, clinical diagnosis, therapeutic interventions, disease management, and patient-centered clinical studies. • Pharmacy Research: pharmacology, pharmaceutics, pharmaceutical chemistry, clinical pharmacy, drug safety, and rational use of medicines. • Biomedical and Laboratory Sciences: pathology, microbiology, physiology, molecular medicine, toxicology, and related translational or laboratory-based investigations. • Public and Applied Health: health systems, community health, medication adherence, healthcare delivery, and applied studies relevant to medical and pharmaceutical practice. • Interdisciplinary Health Research: studies bridging medicine, pharmacy, and allied health sciences with clear relevance to clinical or therapeutic outcomes. Priority is given to original research articles that demonstrate methodological rigor, ethical compliance, and a clear contribution to clinical medicine, pharmaceutical science, or evidence-based healthcare practice.
Articles
57 Documents
Phytochemicals and Antimicrobial Analysis of Ixora Coccinea Flower Extract
Gani J;
Nkafamiya I. I;
Akinterinwa A
African Journal of Clinical Medicine and Pharmacy Research Vol 1 No 1 (2024): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys
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DOI: 10.58578/ajcmpr.v1i1.4144
Ixora coccinea, also known as jungle geranium, is a flowering plant from the Rubiaceae family. It is a tiny, bushy shrub that produces beautiful scarlet flowers and is commonly grown for its ornamental qualities. In addition to its visual appeal, Ixora coccinea has long been employed in a variety of therapeutic traditions due to its potential health advantages. The aim of this work was to conduct phytochemical screening and antimicrobial analysis on a methanolic extract of the flower of Ixora Coccinea. Phytochemicals are plant-derived bioactive molecules that have been proved to offer a variety of health benefits. The phytochemical screening of the methanolic extract identified alkaloids, glycosides, proteins, tannins, saponins, phenols, terpenoids, and steroids while flavonoids was absent. The antimicrobial results of the investigation demonstrated that Ixora coccinea floral extract had strong antibacterial activity against a variety of pathogens. Minimum inhibitory concentration (MIC) values for the extract ranged from 25 % to 75 % while the minimum bactericidal concentration (MBC) values for active extract ranged from 15 to 37 mg/ml. The extract had inhibitory effects on both Gram-positive and Gram-negative bacteria, as well as fungal strains. These findings indicate that the phytochemicals found in Ixora coccinea have powerful antibacterial activities that could be used for medicinal purposes.
Epidemiology of Antibiotic Multidrug Resistant genes in Gram Negative Bacteria among Symptomatic Patients with Bacteriuria Attending Federal Medical Center Yenagoa Bayelsa State Nigeria
Oburu V. O;
Anyiam I. V
African Journal of Clinical Medicine and Pharmacy Research Vol 2 No 1 (2025): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys
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DOI: 10.58578/ajcmpr.v2i1.4273
The global rise in antibiotic resistance among Gram-negative bacteria poses a significant threat to public health, particularly in resource-limited settings. This study investigates the epidemiology of multidrug-resistant (MDR) genes in Gram-negative bacteria isolated from symptomatic patients with bacteriuria at the Federal Medical Center Yenagoa, Bayelsa State, Nigeria. A total of 100 urine specimens were collected from patients within the age range of 13 to 70 years attending Federal Medical Centre Yenagoa. The specimens were analyzed by culture, biochemical tests, and molecular methods. The Antibiotic susceptibility test was done using the Kirby-Bauer disc diffusion method following clinical and laboratory standard institute (CLSI) guidelines. The results showed that 26 bacterial isolates were obtained which include; Escherichia coli 14(53.8%), Klebsiella pneumoniae 6(23.1%), Proteus mirabilis 4 (15.4%), and Pseudomonas aeruginosa 2(7.7%). The highest occurring isolate was E. coli 14(53.8%) while the lowest was P. aeruginosa 2(7.7%). E. coli is the predominant isolate in females 9(34.6%). Female had the highest occurring isolates 14(53.5%) compared to male 12(46.1%). The age ranges with the highest isolates was 51-60 years and 61-70 years with 7(26.9%) respectively, while the lowest was within the age range of 13-20 years with 1(3.8%). The antibiotic susceptibility tests revealed that E. coli showed the highest resistance to ampicillin (85.7%), ceftriaxone (78.57%), piperacillin/tazobactam (64.28%), and cotrimoxazole (64.28%), with notable susceptibility to ciprofloxacin (57.14%) and levofloxacin. K. pneumoniae demonstrated strong resistance to ampicillin and piperacillin/tazobactam at 83.33% respectively, while being most susceptible to ciprofloxacin, gentamicin, and azithromycin at 66.7% respectively. P. mirabilis exhibited high resistance to ceftriaxone, tetracycline, and azithromycin (75%) respectively, but showed maximum susceptibility to piperacillin/tazobactam, ciprofloxacin, gentamicin, and levofloxacin (75%) respectively. P. aeruginosa was most resistant and completely susceptible to multiple antibiotics at 50% and 100%. The resistance genes CTX-M and SHV were present in P. mirabilis while TEM was absent. CTX-M, SHV and TEM were not present in E. coli, K. pneumoniae, and P. aeruginosa. The 16S rRNA of the isolates showed a percentage similarity to other species at 100%. The evolutionary distances computed using the Jukes-Cantor method were in agreement with the phylogenetic placement of the 16S rRNA of the isolates within the E. coli, Klebsiella, Pseudomonas, and Providencia sp., and revealed a close relatedness to the E. coli, K. pneumoniae, P. aeruginosa, and Providencia stuartii. In conclusion, the observed antibiogram profile revealed multidrug resistance among the isolated bacteria and at the same time pathogenic. Therefore, it is recommended to conduct comprehensive surveillance, investigate risk factors, utilize molecular techniques, evaluate antimicrobial stewardship, and promote one health approach to prevent bacteriuria.
Molecular Analysis and Detection of Antibiotic Resistance Genes in Aerobic Bacteria Isolated from Bacteremia Patients at Tertiary Healthcare Institutions
George W. O;
Anyiam I. V
African Journal of Clinical Medicine and Pharmacy Research Vol 2 No 1 (2025): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys
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DOI: 10.58578/ajcmpr.v2i1.4274
Bacteremia is the presence of bacteria in the bloodstream. Aerobic bacteria, such as Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus, are common causes of bacteremia and have developed resistance to various antibiotics. The study aims to analyze and detect antibiotic resistance genes of aerobic bacteria isolated from bacteremia patients in tertiary healthcare institutions. A total of 110 blood specimens were collected from patients attending Federal Medical Centre Yenagoa and Gloryland INRI Medical Centre Yenagoa. The specimens were analyzed by culture, biochemical test, and molecular methods. Out of the 110 blood cultures, 4.5% were positive. Organisms isolated were Staphylococcus aureus (60%) and Klebsiella pneumoniae (40%). The isolates were more common in males (80%) than females (20%). The prevalence of bacteremia between children and adults was 5% and 4.4% respectively. Antibiotic susceptibility test the gram positive isolates showed maximum resistance to ampiclox (100%), zinnacef (100%), erythromycin (100%), and 66.7% resistance to amoxacillin and rocephin respectively. However, high susceptibility was shown to gentamicin (100%), levofloxacin (100%), and ciprofloxacin (66.7%). The gram negative isolates showed maximum resistance to ciprofloxacin (100%) and 50% resistance to cefotaxime, gentamicin, chloramphenicol, ceftriaxone, levofloxacin ampicillin/sulbactam and co-trimoxazole respectively. Nevertheless, maximum susceptibility was shown to ofloxacin, tetracycline, and azithromycin with 100% respectively. The molecular detection of antibiotics resistance genes revealed the presence of CTX-M (100%), and QnRA (33.3%) in the S. aureus strains. CTX-M (100%), QnRA (100%), and QnRB (50%) were seen in the K. pneumoniae strains. However, the aaC gene was found neither in the S. aureus strains nor K. pneumoniae strains. Phylogenetic analysis using 16s rRNA sequences identified bacterial isolates; S. aureus and K. pneumoniae. These results underscore the complexity of resistance patterns and the molecular mechanisms driving them in the studied pathogens. Crucial effort should be put in place to enhance surveillance and regularly update resistance profiles to guide treatment.
Determinants of Exclusive Breastfeeding Practices Among Mothers of Infants [0-6 Months] Attending Immunization Clinic in University of Port Harcourt Teaching Hospital, Rivers State
Dim Lilian Chinyere;
Imarenezor Edobor Peter Kenneth
African Journal of Clinical Medicine and Pharmacy Research Vol 2 No 1 (2025): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys
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DOI: 10.58578/ajcmpr.v2i1.4592
Exclusive breastfeeding (EBF) for the first six months of life is essential for an infant's optimal growth, immunity, and development. However, global adherence remains inadequate, particularly in developing regions. This study aimed to explore the factors influencing EBF practices among mothers of infants aged 0-6 months attending the immunization clinic at the University of Port Harcourt Teaching Hospital (UPTH) in Rivers State, Nigeria. A cross-sectional approach, data were gathered from 382 mothers through structured questionnaires. Analysis was done using Pearson correlation and chi-square tests. The findings showed that social support from healthcare providers, family and breastfeeding support groups played a crucial role in encouraging EBF. A substantial portion of mothers (47.1%) receive help with breastfeeding decisions from healthcare providers, followed by 39.3% from husbands or relatives and 13.6% from friends. Encouragement from husbands or family members plays a crucial role, with 83.8% of mothers reporting support in this regard. Additionally, 26.2% of mothers participate in breastfeeding support groups, and 75.9% of healthcare providers discuss the benefits of breastfeeding with them, further supporting their decisions. A notable 68.1% of healthcare providers assist with the initiation of breastfeeding, and 39.3% receive post-discharge breastfeeding support. The influence of healthcare providers in the decision to exclusive breastfeeding is significant, with 47.1% attributing their decision to this guidance. The findings show that significant correlations were observed between breastfeeding education and antenatal clinic attendance (r = 0.33, p < 0.001), as well as between belief in breastfeeding benefits and the number of antenatal visits (r = 0.22, sp < 0.01). The findings show that attending antenatal clinics, receiving breastfeeding education, and strong belief in the benefits of breastfeeding were positively associated with the practice of EBF. Additionally, factors like the place of delivery, mode of delivery, and home delivery supervisor also played a significant role in determining exclusive breastfeeding practices. Logistic regression analysis further highlighted that maternal education, healthcare support, and socioeconomic factors were key predictors of EBF practices. Conclusively, this study highlights the importance of social support, breastfeeding education, and healthcare provider encouragement in promoting EBF practices. Targeted interventions that focus on enhancing breastfeeding education during antenatal visits and addressing cultural and healthcare-related barriers are recommended to improve EBF rates among mothers attending UPTH and in Rivers State.
In-vitro Cytotoxic and Anti-vibro Cholerae Activities of Methanol Roots Extracts of Morinda citrifolia L (Noni)
Isaac John Umaru;
Daniel Konka;
Iseko Iyoko Kingsly;
Solomon Ossom Asare;
Okrah George Hassan;
Joseph Oteng;
Tensaba Andes Akafa;
Kerenhappuch Isaac Umaru
African Journal of Clinical Medicine and Pharmacy Research Vol 2 No 1 (2025): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys
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DOI: 10.58578/ajcmpr.v2i1.4720
Knowledge on medicinal properties of plants has extensively been applied all over the World to cure or ameliorate suffering of mankind. The use of remedies from plant origin covers a wide variety of maladies and constitutes an alternative way to antibiotics, which is otherwise no longer promising due to the generalized antibiotic resistance among pathogenic microorganisms. The majority of epidemic Vibrio cholerae strains have become resistant to multiple antimicrobial agents for many reasons, including mutations, horizontal gene transfers, etc. Therefore, secondary means of treatment are needed to overcome this impediment in combating cholera. Morinda citrifolia roots extensively used in traditional medicine. Therefore, the present study aims to evaluate the In-vitro Cytotoxic and Anti-vibro Cholerae Activities of Methanol Roots Extracts of Morinda citrifolia against V. cholerae isolates. Methods: Initially, different concentration of the extracts materials was subjected to qualitative phytochemical screening. Anti-V.cholerae activities were assessed by performing MIC (minimum inhibitory concentration) test using disc diffusion method with different concentrations (1.25, 2.25, 5.00, 8.00, 10.00mg/ml) where standard discs of gentamycin and meropenem (10 μg/disc) were used. Further, Brine shrimp lethality bioassay was done to evaluate the extracts’ cytotoxic activities using different concentrations (5,10, 20, 50, 100, 200, 400, 800μg/ml) against vincristine sulphate. Results: The preliminary screening confirms the presence of convenient phytochemical constituents like flavonoids, alkaloids, glycosides, tannins, etc., and are liable for the emergence of the antibacterial activities in different strains. The minimum inhibitory concentrations of methanolic Morinda citrifolia roots was found to be (a) 1.25, (b) 2.25, (c) 5, (d) 8, and (f) 10 mg/ml, respectively. Moreover, 100% mortality was observed only at the highest concentration (800 μg/ml). In comparison, the rest of the test concentration showed less than 90% mortality at the highest concentration (400 μg/ml). Conclusions: The Morinda citrifolia roots possess significant phytochemicals and showed potential anti-V. cholerae activities with mild toxicity. Hence, Morinda citrifolia (L.) roots may be recommended as potential phytotherapy against cholera disease.
Anticancer effect of Copper Activated Plasma Water on MCF7 Breast Cancer Cells
Isaac John Umaru;
Iseko Iyoko Kingsly;
Edom Favour Ogochukwu;
Tensaba Andes Akafa;
Jackson Soyinka Malantso;
Bilyaminu Habibu;
Kerenhappuch Isaac Umaru
African Journal of Clinical Medicine and Pharmacy Research Vol 2 No 1 (2025): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys
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DOI: 10.58578/ajcmpr.v2i1.4721
Broad biological activities of “plasma-activated water” (PAW) have drawn great attentions recently. Treatment of water using gas discharge plasma led to acidic solutions with excellent and broad antibacterial activity. Because PAW caused severe membrane damages in bacteria and diffused freely in extracellular matrix, PAW also demonstrated good anti-biofilm activity. However, further studies revealed that trace amounts of metal ions (mainly copper) in PAW brought by plasma treatment played key roles in bacteria inactivation. The contribution of metal ions to the antibacterial activity varied among PAWs from different working gases. However, solution acidification caused by reactive species in plasma was essential. The experimental results demonstrated that potential artifacts in reported biological activities of PAWs should be considered. Therefore, Copper has important redox activity and can participate in various biochemical reactions by accepting and donating electrons. As a trace element, thus, Anticancer effect of Copper Activated Plasma Water on MCF7 Breast Cancer Cells. Materials and method, used are a non-thermal micro-hollow cathode discharge (MHCD) was used to generate plasma-activated waters (CU-PAWs), The MCF-7 human breast adenocarcinoma cell line (IBRC C10082), and 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was acquired. Cell Viability Measurements; After 48 h of incubation 0.5 mg/ml MTT (20 μl) was added to the control and experimental cells and the cells were incubated for 3 h in a CO2 incubator at 37 °C, Measurement of Reactive Oxygen Species (ROS) and Flow Cytometry was conducted. Results: The 3- and 4-min CU-PAW reduced MCF-7 cells viability to approximately 62% and 56% of control (p< 0.01), respectively. However, in the cases of 1- and 2-min CU-PAW cell proliferation did not diminish significantly as compared with the control group (p> 0.05). This observation is consistent with earlier studies, which illustrated that plasma irradiation reduced cell viability in a time-dependent manner. Thus, in this research, DOX (0.45 µM) combined with 3- or 4-min CU-PAW killed MCF-7 cell efficiently (44% and 39% cell viability, respectively; p< 0.01) than DOX (54% cell viability) or 3- or 4-min CU-PAW alone (63% and 56% cell viability, respectively). These was in line with a that PAW plus cisplatin at low doses reduced viability of human endometrial carcinoma more effectively than cisplatin or PAW alone. Conclusion: Although further investigations are crucial, CU-PAW combined with DOX could be a promising cancer treatment strategy, contributing to a more positive therapeutic agent.
Sociodemographic Determinants Affecting the Practice of Breast Self-Examination Among Women in Northern Ghana
Joseph Oteng
African Journal of Clinical Medicine and Pharmacy Research Vol 2 No 1 (2025): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys
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DOI: 10.58578/ajcmpr.v2i1.4854
Cancer is a group of diseases in which genetically damaged cells proliferate autonomously. Such cells cannot respond to normal regulatory mechanisms that ensure the intercellular cooperation required in multicellular organisms. Consequently, they continue to proliferate, thereby robbing nearby normal cells of nutrients and eventually crowding surrounding healthy tissue. Breast cancer is the most widespread malignancy among females worldwide, accounting for the greatest percentage of deaths (15.5%) from all cancers in women. It was estimated the number of new cases in 2020 would exceed 2.2 million. The research work showed that the participants were abreast with Breast self-examination (BSE) techniques. And that majority of our respondents have a significantly positive impression of BSE. This tallies with the outcome of a study on affected perception of BSE among female secondary school teachers with high perception of BSE. Regarding age, there is a noticeable trend regarding the increase in age and the mean age. Specifically, women aged 30-39 years have the highest mean BSE practice score (41.8947), followed by those aged 40-49 years (40.3333), while the lowest mean score is observed among women aged 10-19 years (36.0000). This suggests a potential correlation between age and BSE practice, with older women exhibiting higher BSE engagement. In conclusion this study's primary objective is to assess the practice and determinants of breast self-examination among women in Chereponi District, Northern Region of Ghana. It will help other part of the world to create awareness of the importance of Breast cancer examination at the early stage, avoiding complication in feature.
Efficacy and Safety of Triple Synergy Therapy Containing Annona Senegalese, Ciprofloxacin, and Omeprazole for Helicobacter Pylori Initial Treatment
Isaac John Umaru;
Tensaba Andes Akafa;
Ocheifa Matthew Ngbede;
Artu Ishishen John;
Kerenhappuch Isaac Umaru
African Journal of Clinical Medicine and Pharmacy Research Vol 2 No 2 (2025): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys
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DOI: 10.58578/ajcmpr.v2i2.4970
Annona senegalensis is a complex plant, with several active compounds contributing to its medicinal properties. The presence of alkaloids such as annonaine and corossolin, which have been shown to possess anti-inflammatory and analgesic effects, is particularly noteworthy. Flavonoids like quercetin and kaempferol are potent antioxidants that help to reduce oxidative stress, while tannins contribute to the plant’s antimicrobial and anti-inflammatory properties. These compounds, working synergistically, may explain the plant’s potential. Material and methods: This study was conducted in accordance with the Declaration of Helsinki with little modification, The rats were completely randomized, numbered according to the sequence, and randomly enrolled in a ratio of 1:1:1 to receive the triple therapy (A group, Annona senegalensis, ciprofloxacin quadruple therapy (B group as Omeprazole quadruple therapy. Results: Annona Senegalese scheme for initial and rescue treatment of H. pylori infection are not inferior to the guideline-recommended therapy. A study compared the eradication efficacy of the Annona Senegalese quadruple therapy (300 mg, 3 times/day) with that of bismuth quadruple therapy. The eradication rates of H.pylori were 87.5% and 87.1% in PP analysis, with no statistical significance (P >0.05), It was observed that, the eradication rates of H. pylori in response to the new triple therapy consisting of Annona Senegalese’s triple therapy (A. Senegalese 500 mg, Ciprofloxacin 1000 mg, Omeprazole 20 mg, A group), were 70.0% and 81.4% by ITT and PP analyses, respectively. Conclusion: The efficacy of Annona Senegalese triple therapy for the initial eradication of H. pylori is commendable thus be used as a combination in clinical therapy.
Molecular Detection of Carbapenem Resistance in Multi-Drug Resistant Pseudomonas aeruginosa from Patients and Fomites in Federal Medical Centre Keffi, Nigeria
Abdulmumin R. I.;
Ngwai Y. B.;
Nkene I. H.
African Journal of Clinical Medicine and Pharmacy Research Vol 2 No 2 (2025): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys
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DOI: 10.58578/ajcmpr.v2i2.5021
This study is the aimed at the molecular detection of carbapenem resistance in multidrug resistant Pseudomonas aeruginosa from patients and fomites in Federal Medical Centre, Keffi, Nigeria. The study was carried out in Federal Medical Centre, Keffi (FMCK), Nigeria, between the period of February- August, 2020. Material and Methods: A total of 365 samples comprising 265 clinical (urine, wound swab, eye swab, ear swab, high vigal swab (HVS) and endocervical swab (ECS)) and 100 fomites samples were collected from Federal Medical Centre, Keffi, Nigeria. Results: Out of 365 clinical and formitesscreened, FMCK had overall occurrence 42 (11.5%) both in clinical andfomite samples examined. The isolates from both formites and clinical samples were more resistant to nalidixic acid (85.7%) and ampicilin (71.4%) but less resistant to imipenem (33.3%), ofloxacin (42.8%) and streptomycin (45.2%).The occurrence of carbapenemase producing imipenem resistant isolates was 28.5%.The occurrence of blaKPC was 100%.The occurrences of blaVIM and blaOXA genes were 75% each. The occurrence of isolates with co-carriage of blaVIM/KPC/OXA (75%) was higher than blaVIM/KPC, blaKPC/OXAand blaVIM/OXA (50%). Conclusions: The occurrence of the isolates from fomites and clinical samples was high and most antibiotics tested were not effective against the P. aeruginosa isolates.
Evaluation of Common Beliefs and Practices of the Slaughterhouse Butchers Tamale Metropolis Ghana
Solomon Ossom Asare;
Emmanuel Olofu Omanchi;
Amina Jummai Shehu;
Issifu Tahidu;
Dadaniel Konka
African Journal of Clinical Medicine and Pharmacy Research Vol 2 No 2 (2025): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys
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DOI: 10.58578/ajcmpr.v2i2.5022
Meat quality and butcher’s awareness plays a key role in production of hygienic meat and preserving the public health. A structured questionnaire was developed and a total of 150 butcher were surveyed in the pilot study. The aim was to evaluation the common beliefs and practices of the slaughterhouse butchers. The survey revealed that 54.5% of the butchers a family business and does not require any formal training and has No need for registration to work as a butcher. And about 62.6 % Use local implements in the slaughter of animal. With about 58.8 % has the knowledge of protective materials during operation, however, 53.8% practice skinning of animals with local knife and bare hands. Based on the results obtained in the study, it is concluded that most of the butchers required education and awareness training about hygienic meat production, threatful meat borne diseases, sanitary conditions and hygienic slaughter techniques in the butcher shops to safe guard the health of themselves and meat consumers.