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PEMUPUKAN DAN INDUKSI Curcuma mangga Val. UNTUK PENINGKATAN ZAT ANTIKANKER DAN UJI SITOTOKSITASNYA PADA T47D Retno Sunarminingsih Sudibyo; Taryono Taryono
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian ini bertujuan untuk meningkatkan zat antikanker dan uji sitotoksitasnya pada T47D dengan menggunakan metode pemupukan dan induksi pada tanaman Curcuma mangga Val. Penelitian dilaksanakan di Greenhouse milik Pusat Inovasi Agroteknologi Terpadu (PIAT) Universitas Gadjah Mada di Kalitirto, Kecamatan Berbah, Sleman, Yogyakarta. Waktu pelaksanaan awal Januari-Oktober 2019. Pelaksanaan penelitian dibagi menjadi 3 tahap. Tahap pertama penanaman dan pemupukan C. mangga Val. Pemupukan dilakukan menggunakan nitrogren (N) organik dari kompos daun-daun legume, fosfor (P) organik dari pupuk SP36, Kalium (K) dari KCl, dan kombinasi pupuk NPK. Tahap kedua adalah ekstraksi dan penetapan kandungan zat aktif menggunakan alat vaccum-rotavapor dan densitometer. Tahap ketigaadalah Uji sitotoksisitas (MTT Assay) menggunakan bahan Sel T47D, media RPMI, MTT, PBS, SDS, dan bahan-bahan disposable, serta ELISA reader. Hasil penelitian menunjukan bahwa pemupukan N organik pada C. mangga Val. mampu meningkatkan produksi minyak dalam rimpang dan sitotoksisitas minyak terhadap sel kanker payudara T47D. Penghentian pemberian air menjelang masa dorman dan suhu lingkungan yang panas (37oC) mampu menginduksimajunya masa dorman pada tanaman C. mangga Val.INCREASING ANTICANKER SUBSTANCES AND THE Cytotoxicity TEST ON T47DUSING FERTILIZATION AND INDUCTION ON Curcuma Mango Val.This study was aimed at increasing the anticancer agent and conducting a cytotoxicity test on T47D by using fertilization and induction methods on Curcuma Val mango plant. The study was conducted at the Greenhouse of Agro-technology Innovation Center (AIC), Gadjah Mada University in Kalitirto, Berbah, Sleman, Yogyakarta. The study was conducted from January to October 2019. The research was divided into 3 stages. The fi rst stage of planting and fertilizing C. mango Val. Fertilization was carried out using organic nitrogren (N) fromcompost of legume leaves, organic phosphorus (P) from SP36 fertilizer, Potassium (K) from KCl and NPK fertilizer combination. The second step is the extraction and the determination of the active ingredient using a vacuum-rotary evaporator and a densitometer. The third stage is the cytotoxicity test (MTT Assay) using T47D cell material, RPMI media, MTT, PBS, SDS and disposable materials, and ELISA reader. The results showed that organic N fertilizationon C. mango Val. able to increase oil production in rhizomes and cytotoxicity of oil against T47D breast cancer cells. Discontinuation of water delivery prior to dormancy and hot ambient temperature ( 37oC) is able to induce the advancement of dormancy in C. mango Val plants.

KEANEKARAGAMAN ACARINA DI PUSAT INOVASI AGRO TEKNOLOGI MANGUNAN Soenarwan Hery Poerwanto; Anggun Handiani; Dila Hening Windyaraini
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian ini dilakukan untuk mengetahui keanekaragaman Acarina dan faktor lingkungan yang mempengaruhi keberadaan Acarina di Pusat Inovasi Agro Teknologi (PIAT) Mangunan, Yogyakarta. Penelitian ini dilakukan di PIAT Mangunan untuk pengambilan sampel dan Laboratorium Sistematika Hewan bagian Parasitologi Fakultas Biologi UGM untuk preparasi dan identifikasi. Metode yang digunakan adalah ekstraksi sampel menggunakan corong Barlese Tullgren dengan beberapa modifikasi. Sampel diambil pada 3 area vegetasi (sirsak, srikaya, dan sawo) dan setiap area diambil lima titik sampling. Pengukuran lingkungan meliputi kelembaban udara dan suhu udara diukur dengan alat higrometer, pH, dan suhu tanah dengan soil tester digital, serta kelembaban tanah dengan soil tester Takemura DM-5. Acarina yang diperoleh dilihat di bawah mikroskop dan di-mounting dengan larutan hoyer’s. Identifikasi Acarina menggunakan buku identifikasi A Manual of Acarology. Data yang diperoleh dari penelitian dianalisis menggunakan indeks keanekaragaman Shannon-Wiener. Berdasarkan identifikasi Acarina yang telah dilakukan, didapatkan 20 Family dan 28 Genus dan 399 cacah individu. Tingkat keanekaragaman Acarina termasuk dalam kategori sedang. Faktor lingkungan yang mempengaruhi kehadiran Acarina adalah suhu dan kelembaban.THE DIVERSITY OF ACARINA IN AGRO TECHNOLOGY INNOVATION CENTER MANGUNANThis study was aimed at determining the diversity of Acarina and environmental factors that affect the existence Acarina at Agro Technology Innovation Center (ATIC) Mangunan, Yogyakarta. This study was conducting at ATIC Mangunan for sampling and the Animal Systematics Laboratory of the Parasitology, Faculty of Biology, Gajah Mada University for preparation and identification. The method used was sample extraction using Barlese Tullgren funnel with several modifications. Samples were taken at 3 vegetation areas (soursop, sarikaya a.k.a Annona squamosa, and sapodilla) and each area was taken five sampling points. The environmental measurements include air humidity and air temperature measured by means of a hygrometer, pH and soil temperature with digital soil tester, and soil moisture with a soil tester Takemura DM-5. Acarina obtained was seen under a microscope and mounted with Hoyer’s solution. The identification of Acarina using identification book A Manual of Acarology. The data obtained from the study were analyzed using the Shannon-Wiener diversity index. Based on Acarina’s identification, 20 families and 28 genera and 399 individual numbers were obtained. The level of diversity of Acarina is categorized as the medium category. The environmental factors that influence the presence of Acarina are temperature and humidity.

MODIFIKASI KAIN AKTIF ANTIBAKTERI BERBASIS KOMPLEK Mn(II) BASA SCHIFF KITOSAN–SALISILALDEHID Ismiyarto - Ismiyarto; Sesika Novari; Ngadiwiyana Ngadiwiyana; Purbowatiningrum Ria Sarjono; Noor Basid Adiwibawa Prasetya
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian ini bertujuan untuk memperoleh kompleks Mn(II)-basa Schiff kitosan-salisaldehida dan menguji aktivitasnya sebagai bahan aktif kain antibakteri. Metode penelitian dilakukan melalui tiga tahapan. Pada tahap pertama dilakukan sintesis basa Schiff kitosan–salisilaldehid dengan mereaksikan kitosan dengan variasi mol salisilaldehid menggunakan campuran pelarut asam asetat dan etanol, diaduk pada suhu 50oC selama 6 jam. Pada tahap kedua, basa Schiff kitosan–salisilaldehid dijadikan kompleks dengan MnCl2.2H2O menggunakan campuran pelarut asam asetat 2% dan etanol, diaduk pada suhu 60oC selama 12 jam. Pada tahap ketiga dilakukan pelapisan kompleks pada kain katun menggunakan metode spray dan selanjutnya dilakukan uji antibakteri terhadap E. coli (G-) dan S. aureus (G+) menggunakan metode difusi cakram. Hasil penelitian menunjukkan bahwa basa Schiff kitosan–salisilaldehid dengan rasio substitusi paling besar (0,122) diperoleh ketika disintesis menggunakan salisilaldehid 0,00227 mol. Senyawa kompleks Mn(II)-basa Schiff kitosan–salisilaldehid yang terbentuk berupa serbuk berwarna hitam dengan rendemen 66%. Kain katun yang telah dilapisi kompleks Mn(II)-basa Schiff kitosan–salisilaldehid efektif menghambat pertumbuhan bakteri dengan persen daya hambat sebesar 16,02% terhadap bakteri E. coli (G-) dan 14,02% terhadap bakteri S. aureus (G+).MODIFICATION OF ANTIBACTERIAL FABRIC FROM Mn(II) SCHIFF BASE CHITOSAN - SALICYLALDEHYDE COMPLEXThis study was aimed at obtaining the complex of Mn(II)-Schiff base chitosan- salicylaldehyde and evaluating the activity of antibacterial agent on cotton fabric. The study was conducted through three treatment steps. In the first step, chitosan-salicylaldehyde Schiff base was synthesized by reacting chitosan with mole variations of salicylaldehyde and combined with a mixture of acetic acid and ethanol as the solvent which was then stirred at 50oC for 6 hours. In the second step, preparation complexes Mn(II)- Schiff base chitosan-salicylaldehyde was conducted by mixing chitosan-salicylaldehyde Schiff base and MnCl2.2H2O using acetic acid 2% -ethanol as a solvent at 60oC for 12 h. In the third step, the complex was coated onto fabric using spray method and the E. coli (G-) dan S. aureus (G+) antibacterial test was done to the coated fabric using disk diffusion method. The results showed that 0.00227 mole salicylaldehyde lead to the highest substitution ratio of Schiff base chitosan-salicylaldehyde of 0.122. The Mn(II)-Schiff base chitosan-salicylaldehyde complex was obtained as black powder with a yield of 66%. Cotton fabric that has been coated with the complex, effectively inhibits bacterial growth with a percent inhibition against E. coli (G-) and S. aureus (G+) bacteria of 16.02% and 14.02%, respectively.

PRODUKSI PIGMEN DAN ASAM γ-AMINOBUTIRAT (GABA) OLEH Monascus purpureus PADA KONSENTRASI INOKULUM DAN WAKTU INKUBASI YANG BERBEDA Maria Sarah Fadillah; Endang Kusdiyantini; Wijanarka Wijanarka
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian ini bertujuan untuk mengetahui produksi pigmen dan GABA oleh M. purpureus dengan konsentrasi inokulum dan waktu inkubasi yang berbeda. Fermentasi dilakukan menggunakan metode fermentasi cair dengan konsentrasi inokulum serta waktu inkubasi yang berbeda. Pengukuran pigmen intraselular dilakukan dengan mengekstraksi pelet sel menggunakan etanol 95%. Produksi GABA ditentukan menggunakan metode ninhidrin. Spektrofotometer digunakan untuk mengukur pigmen pada panjang gelombang 500, 470, dan 400 nm, sementara GABA pada panjang gelombang 401 nm. Penelitian menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor. Hasil penelitian menunjukkan rerata nilai pigmen merah, jingga dan kuning tertinggi pada pigmen ekstraseluler (P0,05) terjadi pada perlakuan C3, sementara pigmen intraseluler (P0,05) pada perlakuan C1, dengan perlakuan waktu inkubasi (P0,05) W14. Interaksi antar kedua perlakuan (C*W) terjadi pada pigmen ekstraseluler merah dan jingga (P0,05). Produksi GABA tidak berbeda secara signifikan pada pada perlakuan konsentrasi inokulum (P0,05), tetapi berbeda secara signifikan pada perlakuan waktu inkubasi (P0,05) dan perlakuan W14 menunjukkan produksi tertinggi (6,1085 mg/ml). Tidak adanya interaksi antardua perlakuan dalam produksi GABA (P0,05).PRODUCTION OF PIGMENTS AND γ-AMINOBUTYRIC ACID (GABA) BY Monascus purpureusThis study was aimed at examining the production of pigments and GABA by M. purpureus in varied inoculum concentration and incubation time. The fermentation was carried out by submerged fermentation method with inoculum concentration and varied incubation time. Cell pellet was extracted using 95% ethanol for intracellular pigment measurement. GABA production was determined by ninhydrin method. Pigments were measured at 500, 470, and 400 nm wavelength by spectrophotometry, and GABA was measured at 401 nm wavelength. The experimental design was Completely Randomize Design (CRD) factorial with two factors. The higher colour value of extracellular (P0,05) red, orange and yellow pigments showed at C3 while intracellular (P0,05) showed at C1 with incubation time at W14. There are some interactions between two factors (C*W) for red and orange extracellular pigments (P0,05). It was observed that inoculum concentrations have no significant difference (P0,05) for GABA production. In other hand, there is significant difference for incubation time factors (P0,05) with the highest production at W14 (6,1085 mg/ml). There is no interaction between two factors for GABA production (P0,05).

PENGARUH VARIASI TANAMAN BARIER SEBAGAI NATURAL PEST CONTROL TERHADAP POPULASI HAMA DAN PRODUKTIVITAS PADI Tien Aminatun; Budiwati Budiwati; Lili Sugiyarto; Amin Khusnadiyah; Anisa Setya Hidayah; Ema Imtihana
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Tujuan dari penelitian ini adalah untuk menganalisis pengaruh variasi jenis tanaman barier terhadap dinamika populasi serangga hama pada tanaman padi dan produktivitas tanaman padi. Penelitian ini dilakukan di Kebun Percobaan Fakultas Pertanian UGM Banguntapan Bantul. Variabel bebas dari penelitian ini adalah perlakuan variasi tanaman barier yaitu kontrol (tanpa barier), gulma alami, bunga matahari, dan bunga kenikir. Variabel terikatnya adalah dinamika populasi serangga hama dan produktivitas tanaman padi. Pengamatan serangga hama dan musuh alaminya dilakukan terbatas pada pagi hari antara pukul 08.00-11.00 dengan metode scan sampling yang dilakukan pada 3 minggu sekali. Data produktivitas tanaman padi yang diambil meliputi berat kering gabah hasil panen per plot perlakuan. Untuk melihat pengaruh perlakuan variasi jenis tanaman barier terhadap populasi serangga hama tanaman padi dan produktivitas tanaman padi dilakukan uji ANOVA. Hasil penelitian menunjukkan tidak terdapat perbedaan yang signifikan dari pengaruh variasi jenis tanaman barier terhadap perbedaan dinamika populasi serangga hama maupun terhadap produktivitas tanaman padi, tetapi perlu diadakan penelitian lanjutan yang dikaitkan dengan variabel lain yang mempengaruhi produktivitas tanaman padi.THE EFFECT OF BARRIER PLANT VARIATION AS NATURAL PEST CONTROL ON PEST POPULATION AND RICE PRODUCTIONThis study was aimed at analyzing the effect of the barrier plant variation to the population dynamic of rice pest insect and on rice crop productivity. The study was conducted in the experimental Garden of Faculty of Agriculture of Gadjah Mada University in Banguntapan Bantul. The independent variable was the treatment of barrier plant variation, i.e. control (without barrier), natural weed, sunflower and kenikir flower plants, while the dependent variables were population dynamic of pest insect and rice crop productivity. Pest insects and their natural enemies observation was carried out in between 08.00-11.00 am by scan sampling every 3 weeks. The retrieval of crop productivity data was done when the harvest per treatment plot. The data obtained was then tested with ANOVA, to find out the effect of the treatments on population dynamic of rice crop pest, as well as rice crop productivity. The results show that there was no significant difference in the effect of barrier plant variation to the differences in population dynamic of pest insects; as well as in the productivity of rice crop, but it is necessary to conduct advanced research that is associated with other variables that affect rice crop productivity.

ELECTRICITY PRODUCTION FROM FOOD WASTE LEACHATE USING DOUBLE CHAMBER MICROBIAL FUEL CELL Willie Prasidha; Akmal Irfan Majid
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

This study was aimed at evaluating the performance of non-aerated and aerated double chamber microbial fuel cells from food waste leachate. The value of open circuit voltage (OCV) and close circuit voltage (CCV) were taken to analyze power density and current density of both configurations. Two double chamber microbial fuel cells (MFC) with different configurations were developed to produce electricity from food waste leachate and studied for 30 days. Anode and catode were made by uncoated carbon felt and graphite rod. Food waste and water were incubated inside a reactor. After 30 days, the electricity production characteristics between the two configurations were obtained. Both configurations reached the same maximum power density and maximum current density but the aerated MFC showed higher performance of maximum open-circuit voltage (OCV), average power density, and current density than non-aerated MFC. The results show that the supplying continuous dissolved air in the cathode chamber resulted in higher voltage, higher average power density, and higher average current density in double chamber microbial fuel cell.

RESPONS FISIOLOGIS DAN ANATOMI AKAR TANAMAN BAYAM (Amaranthus tricolor L.) TERHADAP CEKAMAN NaCl Ian Prabowo; Diah Rachmawati
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Tujuan dari penelitian ini untuk mengetahui pengaruh cekaman NaCl terhadap pertumbuhan dan anatomi akar tanaman bayam serta mengetahui konsentrasi NaCl yang dapat menghambat pertumbuhan tanaman bayam. Penelitian ini digunakan perlakuan NaCl sebanyak 0, 200, 400, 600 dan 800 mM pada tanaman bayam (Amaranthus tricolor L.), Media tanam berupa campuran tanah dan pupuk kandang dan alkohol, safranin, aseton. Alat yang digunakan adalah med line, cawan poerslen, spektrofotometer dan mikroskop. Parameter yang diukur meliputi tinggi tanaman, jumlah daun, warna daun, panjang akar, jumlah akar, berat basah, berat kering, kadar klorofil, tebal epidermis akar, tebal korteks akar dan diameter stele akar. Data dianalisis dengan uji ANOVA dilanjutkan dengan DMRT taraf kepercayaan 95% menggunakan program SPSS 15. Hasil yang diperoleh menunjukkan penambahan NaCl menyebabkan penurunan pertumbuhan tanaman bayam meliputi tinggi tanaman, jumlah daun, kadar klorofil, rasio tajuk dibanding akar dan menurunkan diameter stele akar. Pertumbuhan tanaman menurun seiring peningkatan konsentrasi NaCl karena NaCl menyebabkan cekaman osmotik yang menghambat penyerapan air dan unsur hara yang diperlukan tanaman untuk proses metabolisme.PHYSIOLOGICAL RESPONSE AND ANATOMY OF ROOTY PLANT [ Amaranthus tricolor L.] AGAINST NaClThe study was aimed at determining the effect of NaCl stress on the growth and anatomy of spinach roots and the concentration of NaCl which can inhibit the growth of spinach plants. This study used 0, 200, 400, 600 and 800 mM NaCl treatments on spinach (Amaranthus tricolor L.), planting media in the form of a mixture of soil and manure and alcohol, safranin, acetone. Med line, poerslen cup, spectrophotometer, and microscope were used in this study. The parameters measured plant height, number of leaves, leaf color, root length, number of roots, wet weight, dry weight, chlorophyll content, root epidermis thickness, root cortex thickness, and root stele diameter. The collected data then were analyzed by ANOVA test followed by DMRT 95% confidence level using the SPSS 15 program. The results obtained showed that the addition of NaCl caused a decrease in spinach plant growth including plant height, the number of leaves, chlorophyll content, the ratio of the crown to root and decreased diameter of root stele. Plant growth decreases with increasing NaCl concentration since NaCl causes osmotic stress. This stress inhibits the absorption of water and nutrients needed by plants for metabolic processes.

PEMODELAN 2D LAPISAN BAWAH PERMUKAAN BERDASARKAN DATA GAYA BERAT MIKRO Supriyadi Supriyadi; Khumaedi Khumaedi; Fajar Setiawan; Teguh MM
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian ini bertujuan untuk mendeskripsikan lapisan bawah permukaan tanah berdasarkan data gaya berat. Pengukuran dilakukan pada bulan Januari 2019. Penelitian menggunakan metode geofisika yaitu metode gaya berat dan metode survei. Setelah dilakukan pengolahan kemudian data anomali gravitasi dapat diinterpretasikan dengan bantuan peta geologi. Alat yang digunakan adalah Gravimeter Scintrex CG-5 Autograv. Hasil penelitian menunjukkan bahwa struktur bawah permukaan dapat diinterpretasikan dalam empat lapisan. Lapisan pertama yaitu top soil yang termasuk formasi aluvium (Qa) dengan densitas rata-rata batuan 2.1 g/cm3 dengan kedalaman 0-50 m. Lapisan kedua memliki densitas rata-rata batuan 2.3 g/cm3 di kedalaman 50-120m. Lapisan ketiga memiliki densitas rata-rata batuan 2.4 g/cm3 di kedalaman 120-195m. Penyusun batuan lapisan pertama hingga ketiga merupakan batuan sedimen seperti lempung, batupasir, dan batu serpih. Lapisan keempat adalah base yang termasuk formasi damar (Qtd) dengan densitas 2,67 g/cm3 di kedalaman lebih dari 195 m dengan batuan penyusun didominasi breksi vulkanik dan tufan halus.2D MODELING SUBSURFACE LAYER BASED ON MICROGRAVITY DATAThis study was aimed at describing the subsurface layer based on the gravity data. The measurements were made in January 2019. The study used the geophysical method, namely the gravity method and the survey method. After being analyzed, the anomaly gravitation data then interpreted using geological maps. Gravimeter Scintrex CG-5 Autograph was used in this study. The results show that subsurface structures can be interpreted in four layers. The first layer is topsoil which includes alluvium (Qa) formation with an average rock density of 2.1 g/cm3 with a depth of 0-50 m. The second layer has an average rock density of 2.3 g/cm3 at a depth of 50-120m. The third layer has an average rock density of 2.4 g/cm3 at a depth of 120-195m. First to third layer rock compilers are sedimentary rocks such as clay, sandstone, and shale. The fourth layer is a base that includes the formation of resin (Qtd) with a density of 2.67 g/cm3 at a depth of more than 195 m with the constituent rocks dominated by volcanic breccias and fine tuffs.

UJI ANTIBAKTERI MASKER KEFIR SUSU KAMBING PADA Staphylococcus epidermidis SECARA IN VITRO Ajeng Desti Ningsih; Nur Khikmah
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian ini bertujuan untuk mengetahui antibakteri masker kefir susu kambing pada Staphylococcus epidermidis dan menghitung jumlah koloni Bakteri Asam Laktat (BAL) dan khamir. Uji antibakteri dilakukan dengan metode difusi sumuran. Antibakteri masker kefir ditunjukkan dengan adanya zona hambat yang terbentuk di sekitar sumuran. Spread plating dilakukan untuk menghitung koloni bakteri asam laktat dan khamir. Medium MRSA dan PDA diinkubasi pada suhu 37ºC selama 2-3 x 24 jam. Jumlah koloni yang tumbuh dihitung menggunakan metode Standard Plate Count dengan jumlah koloni 30-300, dan dinyatakan dalam satuan CFU/g. Hasil penelitian menunjukkan bahwa masker kefir mempunyai sifat bakteriostatik. Kemampuan antibakteri masker kefir susu kambing pada Staphylococcus epidermidis disebabkan karena di dalam supernatan masker kefir terdapat senyawa antibakteri. Hal ini ditandai dengan terbentuknya zona irradikal. Rerata koloni BAL pada masker kefir susu kambing A dan B adalah 1,5×109 dan 1,2×1010 CFU/g. Rerata jumlah koloni khamir pada masker kefir susu kambing A dan B adalah 2,1×1010 dan 3,0×1010 (3,9×1010) CFU/g.ANTIBACTERIAL OF GOAT’S MILK KEFIR MASK ON Staphylococcus epidermidis IN VITROThis study was aimed at determining the antibacterial goat’s milk kefir mask on Staphylococcus epidermidis and the number of Lactic Acid Bacteria (BAL) and the number of yeast colonies. The antibacterial test was carried out using the diffusion method of the wells. The antibacterial activity in the kefir mask is shown by the presence of inhibitory zones that form around the well. Spread plating was done to calculate the colonies of lactic and yeast acid bacteria. MRSA and PDA medium were incubated at 37ºC for 2-3 x 24 hours. The number of growing colonies is calculated using the Standard Plate Count method with the number of colonies of 30-300 and expressed in units of CFU/g. The results showed that kefir masks had bacteriostatic properties. The antibacterial ability of goat’s milk kefir mask was since the kefir supernatant contained antibacterial compounds. This is indicated by the formation of an nonradical zone. The mean of BAL colonies in goat milk masks A and B was 1.5 × 109 and 1.2 × 1010 CFU/g. The average number of yeast colonies in Goat milk masks A and B was 2.1 × 1010 and 3.0 × 1010 (3.9 × 1010) CFU/g.

PENGARUH JENIS INOKULAN DAN DOSIS KOMPOS DALAM FITOREMEDIASI MENGGUNAKAN TANAMAN RAMI Desvia Diyanti Nursyabani; Pudjawati Suryatmana; Rija Sudirja
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian ini bertujuan untuk mengetahui interaksi antara pemberian jenis inokulan dan kompos rami terhadap laju degradasi hidrokarbon, total populasi Azospirillum sp., dan diameter batang rami. Penelitian ini dilaksanakan dari bulan November 2017 sampai dengan Februari 2018 di Laboratorium Biologi Tanah, Laboratorium Kimia Tanah dan Nutrisi Tanaman, Rumah Kaca dan Kebun Percobaan Fakultas Pertanian Universitas Padjadjaran. Penelitian ini menggunakan rancangan percobaan acak kelompok (RAK) faktorial yang terdiri dari dua faktor yaitu jenis inokulan dan dosis kompos. Perhitungan total populasi Azospirillum sp. dengan metode Total Plate Count (TPC) dan pengukuran kadar Total Petroleum Hidrokarbon (TPH) dengan metode gravimetri. Berdasarkan hasil penelitian dapat disimpulkan bahwa faktor perlakuan jenis inokulan dan dosis kompos pada fitoremediasi tanah tercemar hidrokarbon minyak bumi tidak menunjukkan adanya pengaruh interaksi terhadap laju degradasi hidrokarbon, total populasi Azospirillum sp., dan diameter batang tanaman rami. Terjadi pengaruh mandiri faktor perlakuan dosis kompos terhadap laju degradasi hidrokarbon dan diameter batang tanaman rami. Pengaruh mandiri dosis kompos rami memberikan hasil terbaik pada dosis 2,5% (w/w) terhadap peningkatan diameter batang tanaman rami.THE EFFECT OF INOCULANT TYPE AND COMPOST DOSAGE IN PHYTOREMEDIATION PROCESS USING RAMIE PLANTThis study was aimed at determining the interaction between the administration of inoculant types and hemp compost to the rate of hydrocarbon degradation, the total population of Azospirillum sp., and the diameter of the hemp stem. This study was conducted from November 2017 to February 2018 in the Soil Biology Laboratory, Soil Chemistry, and Plant Nutrition Laboratory, Greenhouse and Experimental Gardens, Faculty of Agriculture, Padjadjaran University. This study used a Randomized Complete Block Design (RCBD) consisting of two factors: the type of inoculant and compost dose. Calculation of the total population of Azospirillum sp. by the Total Plate Count (TPC) method and measurement of Total Petroleum Hydrocarbon (TPH) levels by the gravimetric method. Based on the results, it can be concluded that the factor of inoculant treatment and compost dosage in phytoremediation of petroleum hydrocarbon polluted soils does not show any interaction effect on the rate of hydrocarbon degradation, total population of Azospirillum sp., and stem diameter of hemp plants. There is an independent effect of compost dose treatment factor on the rate of hydrocarbon degradation and stem diameter of the hemp plant. The independent effect of hemp compost dose gives the best results at a dose of 2.5% (w/w) to increase the diameter of the hemp plant stems.

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Editorial Address
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Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA