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PERENDAMAN BERBAGAI DOSIS EKSTRAK DAUN BAKAU (Rhizophora apiculata) UNTUK PENGOBATAN KEPITING BAKAU (Scylla serrata) YANG DIINFEKSI BAKTERI Vibrio harveyi Putri, Amanda Mega; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 4, Nomor 4, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Budidaya kepiting bakau (Scylla serrata) sering terkendala karena serangan penyakit bakterial Vibrio harveyi. Penggunaan antibiotik dalam jangka panjang dapat menimbulkan resistensi dan mencemari lingkungan. Penggunaan bahan alami untuk pengobatan infeksi bakteri sedang digalakkan, salah satunya adalah ekstrak daun bakau (Rhizophora apiculata). Penelitian ini bertujuan untuk mengetahui pengaruh perendaman ekstrak daun bakau terhadap kelulushidupan, laju pertumbuhan spesifik, dan histopatologi hepatopankreas kepiting serta mengetahui dosis terbaik untuk mengobati kepiting yang diinfeksi bakteri V. harveyi. Kepiting bakau yang digunakan berjumlah 48 ekor dengan berat tubuh antara 40.11Â±2.53 gram, yang kemudian disuntik bakteri V. harveyi dengan kepadatan 106 CFU/ml secara intramuskular. Perendaman ekstrak daun bakau dilakukan 60 â€“ 90 menit pasca penyuntikan setelah muncul gejala klinis seperti merenggangnya kaki renang dan kaki jalan, karapas menghitam, terdapat bintik putih, dan munculnya bercak merah. Perendaman ekstrak daun bakau berpengaruh sangat nyata (P<0.05) dan P(<0.01) terhadap kelulushidupan kepiting yang diinfeksi bakteri V. harveyi. Nilai rerata kelulushidupan tertinggi hingga terendah berturut-turut yaitu 100% (perlakuan D), 66.67% (perlakuan C), 58.33% (perlakuan B), dan 41.67% (perlakuan A). Sebaliknya, perendaman ekstrak daun bakau tidak memberikan pengaruh nyata (P>0.05) dan P(>0.01) terhadap pertumbuhan kepiting bakau. Hasil pengamatan histopatologi diperoleh adanya kerusakan berupa nekrosis dan vakuolisasi pada organ hepatopankreas. Pada penelitian ini, dosis 900 ppm merupakan dosis terbaik dalam mengobati kepiting bakau yang diinfeksi V. harveyi yang mampu memberikan efek penyembuhan terhadap kepiting bakau.Â Mud crab (Scylla serrata) culture often found obstacle due to bacterial infection Vibrio harveyi. Long-term use of antibiotics cause bacterial resistante and contaminated the environment. Natural ingredients has been for treatment of diseased crab, one of which was mangrove leaf extract (Rhizophora apiculata). This research was aimed to observe the effect of immersion mangrove leaf extract to the survival rate, specific growth rate, and histopathological picture of hepatopancreas mud crab and to know the best dose to treat mud crab that was infected by bacteria V. harveyi. Mud crab used in this research was 48 animals Â with body weight between 40.11Â±2.53 grams, then injected by bacteria V. harveyi with a density of 106 CFU/ml intramuscularly. Dipping in mangrove leaf extract was conducted for 60 â€“ 90 minutes after injection with the appearance of clinical signs such as wide apart swimming and walking legs, blackened carapace, white spots, and red spots. Dipping of mangrove leaf extract significantly effect (P<0.05) and P(<0.01) on the survival of mud crab that was infected by bacteria V. harveyi. The average value of highest to the lowest survival 100% (treatment D), 66.67% (treatment C), 58.33% (treatment B), and 41.67% (treatment A) respectively. The other way, the submersion with mangrove leaf extract showed not giving significant different (P>0.05) and P(>0.01) on growth of mud crab. Histopathological observations obtained were necrosis and vakuolisation in hepatopancreas organ. In this research, dose of 900 ppm give the best treatment to mud crab (S. serrata) were infected V. harveyi. Therefore, immersion mangrove (R. apiculata) leaf extract was able to provides a healing effect on mud crab infected bacteria V. harveyi.

PENGGUNAAN EKSTRAK JAHE MERAH (Zingiber officinale var. Rubrum) SEBAGAI ALTERNATIF PENGOBATAN IKAN NILA (Oreochromis niloticus) YANG DIINFEKSI BAKTERI Aeromonas hydrophila Indriani, Aprilia Dwi; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Volume 3, Nomor 3, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Ikan nila (Oreochromis niloticus) merupakan jenis ikan air tawar yang mudah dipelihara. Intensifikasi budidaya dapat menyebabkan serangan penyakit bakteri seperti Aeromonas hydrophila. Hal ini dapat menyebabkan kerugian ekonomi dalam kegiatan budidaya. Upaya yang dilakukan untuk mengobati serangan bakteri A. hydrophila salah satunya menggunakan bahan alami, ekstrak jahe merah (Zingiber officinale var. Rubrum). Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan ekstrak jahe merah untuk mengobati ikan nila yang diinfeksiÂ A. hydrophila. Ikan nila yang digunakan sebanyak 120 ekor dengan ukuran 7 â€“ 9 cm kemudian disuntik bakteri A. hydrophila dengan kepadatan 108 CFU/ml secara intramuskular. Pelarut yang digunakan dalam ekstraksi jahe merah adalah ethanol 96%. Pengobatan ini menggunakan metode short bathing selama 8 menit dan dilakukan sebanyak 2 kali selama penelitian, yaitu hari pertama dan hari ke 8 setelah penyuntikan. Penggunaan ektrak jahe merah berpengaruh nyata (P<0,05) untuk mengobati ikan nila yang diinfeksi A. hydrophila dan ekstrak ini mampu menyembuhkan luka (ulcer) pada ikan nila yang diinfeksi A. hydrophila dalam 14 hari. Nilai rata-rata kelulushisupan tertinggi hingga terendah berturut-turut yaitu 57,78% (perlakuan C), 46,71% (perlakuan B), 40,78% (perlakuan D) dan 8,85% (perlakuan A).Â Tilapia (O. niloticus)Â is one of the freshwater species which is easy to be cultured. Intensification of Tilapia causes bacterial diseases, such as A. hydrophila. This case resulted in economic disadvantage in aquaculture. One of the effort used to eliminate A. hydrophila infection was the usage of natural ingredient, red ginger (Zingiber officinale var. Rubrum) extract. The aims of this research was to determine the effect of red ginger extract for healing Tilapia infected by A. hydrophila. The Tilapia used were 120 fish, around 7 â€“ 9 cm in size and were injected by bacteria A. hydrophila with density 108CFU / mL intramuscularly. Red ginger extract was obtained by dipping in ethanol 96%. This treatment used short bathing method for 8 minutes and it was done as much as 2 times, at the 1st and 8th days post-injection. The utilization of red ginger extract showed a significant effect (P<0.05) for healing Tilapia infected by A. hydrophila and this extract could heal ulcer on Tilapia infected by A. hydrophila in 14 days. The results showed that the average value of the highest to the lowest survival in a row were 57.78% (treatment C), 46.71% (treatment B), 40.78% (treatment D) and 8.85% (treatment A) respectively.

STUDY KEBERADAAN EKTOPARASIT Octolasmis sp. PADA KEPITING BAKAU (Scylla serrata) JANTAN DAN BETINA PADA PERTAMBAKAN SEMARANG Wardhani, Catleya Kusuma; Sarjito, - -; Haditomo, Alfabetian Harjuno Condro
Journal of Aquaculture Management and Technology Volume 7, Nomor 1, Tahun 2018
Publisher : Journal of Aquaculture Management and Technology

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Kepiting bakau (Scylla serrata) merupakan salah satu komoditas andalan perikanan yang bernilai ekonomis tinggi, namun kendala yang sering dihadapi dalam proses perkembangan budidaya adalah infeksi parasit yang dapat menurunkan produksi budidaya yaitu parasit Octolasmis sp. Tujuan penelitian ini adalah mengetahui jenis-jenis parasit Octolasmis sp. yang menginfeksi kepiting bakau di pertambakan Semarang dan mengetahui tingkat infestasi parasit Octolasmis sp. pada kepiting jantan dan betina. Peneltian ini menggunakan metode eksploratif dan teknik metode random sampling. Kepiting yang diidentifikasi sebanyak 100 ekor dengan perbandingan jantan dan betina 1 : 1 yang diperoleh dari 4 sumber tambak dengan masing-masing berjumlah 25 ekor kepiting, dengan ukuran panjang karapaks7-11 cm dengan nilai rerata panjang karapaks 8,6 ± 0,73cm dan beratbobot 54-188 g dengan nila irerata112,68 ± 23,29 g. Hasil penelitian menunjukan bahwa jenis Octolasmis sp. yang teridentifikasi menyerang kepiting bakau pada pertambakan Semarang yaitu Octolasmis cor dan Octolasmis angulata. Tingkat infeksi parasit Octolasmis berdasarkan jumlah parasit yang ditemukan, pada kepiting betina parasit Octolasmis menginfeksi sebesar 68,09% dengan jumlah parasit O. cor sebesar 36,7% dan jumlah O. angulata sebesar 31,37%, sedangkan pada kepiting jantan parasit Octolasmis menginfeksi sebesar 31,9% dengan jumlah parasit O. cor sebesar 18,42% dan jumlah O. angulata sebesar 13,49%. Tingkat infeksi parasit Octolasmis berdasarkan jumlah parasit yang ditemukan lebih banyak menginfeksi kepiting betina daripada kepiting jantan.Mud crab (Scyllaserrata) is aaquaculture commodity that has a high economic value, however the constraints that are often found in aquaculture process is parasite infection, that can reduce the production of aquaculture is called parasite Octolasmissp.. The purpose of this study was to determine the types of parasites Octolasmissp. who infects mud crabs in Semarang ponds and to determine the level of Octolasmissp. parasite infestation between male and female crabs.This research uses explorative method and random sampling method technique. The crab identified as many as 100 crabs with a ratio of male and female 1:1 obtained from 4 sources of pounds with 25 crabs each, with leght of carapace is 7-11 cm with the average length is 8.6 ± 0.73 cm and the weight is 54-188 g with the mean of weight is 112.68 ± 23.29 g.The results showed that the type of Octolasmissp. who were identified to attack mud crabs at Semarang pounds is Octolasmiscor and Octolasmis angulata. Octolasmis parasite infection rate based on the number of parasites found, on female crab Octolasmis parasite infected 68.09% with the number of parasites O. cor is 36.7% and the number of O. angulata is 31.37%, meanwhile on male crab Octolasmis parasite infected 31.9% with the number of parasites O. cor is 18.42% and the number of O. angulata is 13.49%. The rate of parasitic infection of Octolasmis based on the number of parasites found more infects female crabs than male crabs.Mud crab (Scyllaserrata) is aaquaculture commodity that has a high economic value, however the constraints that are often found in aquaculture process is parasite infection, that can reduce the production of aquaculture is called parasite Octolasmissp.. The purpose of this study was to determine the types of parasites Octolasmissp. who infects mud crabs in Semarang ponds and to determine the level of Octolasmissp. parasite infestation between male and female crabs.This research uses explorative method and random sampling method technique. The crab identified as many as 100 crabs with a ratio of male and female 1:1 obtained from 4 sources of pounds with 25 crabs each, with leght of carapace is 7-11 cm with the average length is 8.6 ± 0.73 cm and the weight is 54-188 g with the mean of weight is 112.68 ± 23.29 g.The results showed that the type of Octolasmissp. who were identified to attack mud crabs at Semarang pounds is Octolasmiscor and Octolasmis angulata. Octolasmis parasite infection rate based on the number of parasites found, on female crab Octolasmis parasite infected 68.09% with the number of parasites O. cor is 36.7% and the number of O. angulata is 31.37%, meanwhile on male crab Octolasmis parasite infected 31.9% with the number of parasites O. cor is 18.42% and the number of O. angulata is 13.49%. The rate of parasitic infection of Octolasmis based on the number of parasites found more infects female crabs than male crabs.

EFIKASI PERENDAMAN EKSTRAK SAMBILOTO (Andrographis paniculata Ness) DENGAN SALINITAS BERBEDA DAN PENGARUHNYA PADA KELULUSHIDUPAN SERTA INDEKS FAGOSITOSIS IKAN NILA (Oreochromis niloticus) YANG DIINFEKSI Aeromonas hydrophila Darma, Rahmi Gusti; Sarjito, -; Haditomo, Alfabetian Harjuno Condro
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Ikan nila (O. niloticus) merupakan ikan air tawar yang dapat hidup pada air bersalinitas. Salinitas dipengaruhi oleh reaksi osmotik dalam tubuh ikan tetapi kemampuan yang dimilliki ikan nila untuk mempertahankan salinitas berbeda tidak mampu melindungi dari serangan bakteri. Upaya yang dilakukan untuk mencegah serangan bakteri A. hydrophila salah satunya menggunakan bahan alami yaitu ekstrak sambiloto (A. paniculata Ness) yang dimasukan pada media salinitas berbeda. Penelitian ini bertujuan mengetahui konsentrasi salinitas yang paling efektif untuk menyerap ekstrak sambiloto dan pengaruh ekstrak sambiloto terhadap kelulushidupan ikan nila yang diinfeksi A.hydrophila. Ikan nila yang digunakan sebanyak 120 ekor dengan ukuran 7,5Â±0,03 cm. Perendaman ikan dengan salinitas 0, 5, 10, dan 15 ppt. Dosis ekstrak yang digunakan adalah 100 ppm dalam 5 L air dengan 3 kali ulangan dan direndam selama 5 jam. Setelah itu ikan disuntik bakteri A. hydrophila dengan kepadatan 108 CFU/ml. Nilai rata-rata kelulushidupan tertinggi hingga terendah berturut-turut yaitu 81,14% (perlakuan C), 78,93% (perlakuan D), 72,29% (perlakuan B) dan 54,70% (perlakuan A). Perendaman ikan dengan salinitas berbeda tidak berpengaruh nyata terhadap kelulushidupan ikan nila yang diinfeksi A. hydrophila. Perendaman ikan pada salinitas 10 ppt yang ditambahkan ekstrak sambiltoto merupakan perendaman terbaik yang memberi pengaruh pada indeks fagositosis. Diketahui nilai tertinggi indeks fagositosis perlakuan C (70,33%).Â Â Tilapia (O. niloticus) is one of fish fresh water can live in the salinity. Salinity affected by osmotic reaction in fish body but the ability in tilapia for maintain by different salinity not able to protect bacterial desease. Efforts are being made to prevent bacterial attack one A. hydrophila using natural ingredients that extracted from sambiloto (A. paniculata Ness) and put into in different salinity media. The aim of this study to determine the effective concentration of salinity to absorb the effect of the extract of sambiloto and effect of sambiloto extract on survival of tilapia which has infected by A.hydrophila. 120 Tilapia this research used with lenght of 7.5 Â± 0.03 cm. The treatment used with a salinity of 0, 5, 10, and 15 ppt . With extract dose 100 ppm in 5 L of water with 3 replications and deeping for 5 hours . After that, the Â fishes were injected with bacteria A. Hydrophila in density of 108 CFU / ml . The average value of the highest survival rate to the lowest row is 81.14 % ( treatment C ), 78.93 % ( treatment D ), 72.29 %Â ( treatment B ) and 54.70 % ( treatment A ) . The deeping treatment in different salinity did not significantly affect survival of tilapia which infected by A. hydrophila . Soaking the fish at 10 ppt salinity with sambiltoto extract gives the best immersion effect on phagocytosis index. It is discovered that the highest value of phagocytosis index treatment C ( 70.33 % ) .

PENGARUH KAROTENOID DARI TEPUNG ALGA Haematococcus pluvialis DAN MARIGOLD BERBASIS ISOKAROTENOID PADA PAKAN BUATAN TERHADAP KECERAHAN WARNA ORANYE, EFISIENSI PEMANFAATAN PAKAN DAN PERTUMBUHAN IKAN MAS KOKI (Carassius auratus) Uly, Marta; Pinandoyo, - -; Hastuti, Sri
Journal of Aquaculture Management and Technology Volume 6, Nomor 3, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

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Ikan mas koki (Carassius auratus) merupakan salah satu ikan hias yang banyak diminati karena keindahan warnanya. Â Warna ikan mas koki didapatkan dari karotenoid yang terkandung dalam pakan. Â Karotenoid ini berasal hewan dan tumbuhan yang mengandung pigmen warna seperti tepung alga H. puvialis dan marigold. Â Penelitian ini bertujuan untuk mengetahui pengaruh karotenoid dari tepung alga H. puvialis dan marigold terhadap peningkatan kecerahan warna oranye ikan mas koki, mengetahuiÂ pengaruh pemberian karotenoid dari tepung alga H. pluvialis dan marigold terhadap total konsumsi pakan, rasio konversi paka, laju pertumbuhan spesifik, dan tingkat kelulushidupan ikan mas koki, dan mengetahui jenis bahan terbaik dalam meningkatkan kecerahan warna oranye ikan mas koki. Â Padat tebar ikan mas koki yaitu 1 ekor/l. Â Penelitian ini menggunakan metode eksperimental dengan 4 perlakuan dan 3 ulangan. Â Perlakuan dalam penelitian ini yaitu Perlakuan A (penambahan karotenoid 0 mg/kg), B (penambahan karotenoid 200 mg/kg dari tepung alga H. puvialis), C (penambahan karotenoid 200 mg/kg dari tepung marigold), dan D (penambahan karotenoid 100 mg/kg dari tepung alga H. puvialis dan 100 mg/kg dari tepung marigold). Â Data yang diamati meliputi nilai hue, rasio konversi pakan, laju pertumbuhan spesifik, tingkat kelulushidupan, dan kualitas air. Â Hasil penelitian menunjukkan bahwa penambahan karotenoid pada pakan buatan berpengaruh sangat nyata nilai hue dan FCR namun tidak berpengaruh nyata terhadap laju pertumbuhan spesifik dan tingkat kelulushidupan. Â Perlakuan C (penambahan karotenoid 200 mg/kg dari tepung marigold) yang menghasilkan tingkat penurunan nilai hue terbesar yaitu 4,82o.Â Goldfish (C. auratus) is one of the ornamental fish that are much sought because of the beauty of its color.Â The color of ornamental fish is obtain from carotenoids contained within feed. Â Carotenoids are derive from animals and plants that contain color pigments such as flour H. pluvialis of algae and marigold. Â This research purposed to know effect of addition carotenoids from flour H. pluvialis of algae and marigold towards increased the brightness orange color of goldfish, effect of addition carotenoids from flour H. pluvialis of algae and marigold towards total feed consumption, feed conversion ratio, spesific groeth rate, and survival rate of gold fish, and to know the best type of material in improving the brightness orange color of goldfish. Â The density of goldfish was 1 tail/l. This research used experimental method with complete randomize design of 4 treatments and 3 repetitions. Â The treatments of this research were Treatment A (addition carotenoids 0 mg/kg), B (addition carotenoids 200 mg/kg from flour H. pluvialis of algae), C (addition carotenoids 200 mg/kg from flour marigold), and D (addition carotenoids 100 mg/kg from flour H. pluvialis of algae and 100 mg/kg from flour marigold). Â Data that observed during this research were value of hue, feed conversion ratio, spesific growth rate, survival rate, and water quality. Â The results of this research showed that the addition carotenoid in feed give significant different towards value of hue and feed conversion ratio but not sigficant different Â towards spesific growth rate and survival rate. Â Treatment C (addition carotenoid 200 mg/kg from flour marigold) produced a reduced rate of the biggest value of hue was 4,82o.

Performa Profil Darah Lele Dumbo (Clarias gariepinus) Yang Terserang Penyakit Kuning Setelah Pemeliharaan Dengan Penambahan Vitamin C Pada Pakan Hartanti, Siwi; Hastuti, Sri; -, Sarjito
Journal of Aquaculture Management and Technology Vol 2, No 1 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstrak Vitamin C yang ditambahkan dalam pakan diharapkan dapat mengurangi stress dan mempercepat proses penyembuhan luka pada ikan. Tujuan dari penelitian ini adalah untuk mengetahui performa profil darah pada lele dumbo (Clarias gariepinus) yang terserang penyakit kuning setelah penambahan vitamin C pada pakan dan mengetahui nilai profil darah setelah penambahan vitamin C pada pakan. Hasil penelitian menunjukkan bahwa pemberian vitamin C tidak berpengaruh nyata (P>0,05) terhadap nilai profil darah ikan lele dumbo (C. gariepinus). Nilai eritrosit tertinggi pada perlakuan A sebesar 2,02Â±0,26 x106 sel/ul, nilai leukosit tertinggi pada perlakuan A sebesar 102Â±9,86 x103 sel/ul, nilai hemoglobin tertinggi pada perlakuan A sebesar 7,9Â±1,03 g/dl, nilai hematokrit tertinggi pada perlakuan A sebesar 24,07Â±3,83 %, nilai glukosa darah tertinggi pada perlakuan D sebesar 48,67Â±4,04 mb/L, nilai biliriubin direk tertinggi pada perlakuan AÂ sebesar 0,17 mg/dl, bilirubin indirek nilai tertinggi pada perlakuan B, C dan DÂ sebesar 0,13 mg/dl, nilai bilirubin total tertinggi pada perlakuan C sebesar 0,3 mg/dl, nilai SGPT tertinggi ada pada perlakuan C sebesar 21,33Â±2,08 U/L dan pada nilai SGOT tertinggi ada pada perlakuan B sebesar 103,67Â±21,50 U/L. Hasil pengamatan jaringan hati ikan lele dumbo kuning menujukkan perubahan struktur hati pada semua perlakuan selama 4 minggu. ABSTRACT Vitamin C which was added in feed was expected to reduce stress and accelerate the process of healing wounds in fish. The purpose of this research is to find out the performence profile of blood dumbo catfish (Clarias gariepinus) by the yellow disease after increment vitamin C on feed and find out the profile blood value after increment vitamin C on feed. The result from this research, that vitamin C addition was not significantly affectÂ (P>0,05) to the profile of dumbo cathfish (C. gariepinus) blood sample. The highest Erythrocytes value of treatment A was 2,02Â±0,26 x106 sel/ul, the highest leukocyte value of treatment A was 102Â±9,86 x103 sel/ul, the highest hemoglobin value of treatment A was 7,9Â±1,03 g/dl, the highest Hematokrit value of treatment A was 24,07Â±3,83 %, the highest blood glucose value ofÂ treatment D was 48,67Â±4,04 mb/L, the highest direct biliriubin value of treatment A was 0,17 mg/dl, the highest indirect Bilirubin value of treatment B, C and D were 0,13 mg/dl, The highest value of total of bilirubin in treatment C was 0,3 mg/dl, the highest value ogÂ SGPT was exist inÂ treatment C of 21,33Â±2,08 U/L, and the highest value of SGOT was exist in treatment B as much as 103,67Â±21,50 U/L. The results from observation of heart tissue catfishes which were affected by theÂ yellow disease,Â indicated stucture changedÂ of its heart in all treatment for four weeks. Â

PENGARUH PENAMBAHAN ENZIM PAPAIN PADA PAKAN KOMERSIAL TERHADAP EFISIENSI PEMANFAATAN PAKAN, LAJU PERTUMBUHAN DAN KELULUSHIDUPAN IKAN SIDAT (Anguilla bicolor) Sagita, Fadil; Rachmawati, Diana; Suminto, - -
Journal of Aquaculture Management and Technology Volume 6, Nomor 4, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

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Ikan sidat (Anguilla bicolor) merupakan jenis ikan yang memiliki pertumbuhan lambat karena daya cernanya yang rendah. Salah satu usaha yang dapat dilakukan untuk meningkatkan pencernaan pakan adalah dengan penambahan enzim eksogenus ke dalam pakan ikan sidat. Enzim eksogenus yang sering ditambahkan ke dalam pakan adalah enzim papain. Penambahan enzim papain pada pakan komersial diduga dapat mengoptimalkan pencernaan ikan sidat (A. bicolor) melalui aktivitas proteolitiknya. Tujuan dari penelitian ini adalah mengetahui pengaruh dan dosis terbaik penambahan enzim papain pada pakan komersial terhadap efisiensi pemanfaatan pakan, pertumbuhan dan kelulushidupan ikan sidat (A. bicolor). Ikan uji yang digunakan dalam penelitian ini adalah elver ikan sidat (A. bicolor) sebanyak 120 ekor dengan bobot rata â€“ rata 6,05Â±1,17 g/ekor. Wadah pemeliharaan yang digunakan berupa akuarium dengan volume 70 liter sebanyak 12 buah. Penelitian dilakukan dengan metode eksperimental RAL dengan satu faktor yaitu dosis enzim papain berbeda yang diulang sebanyak tiga kali. Perlakuan yang digunakan adalah A (dosis enzim papainÂ 0%/kg pakan), B (dosis enzim papain 0,85%/kg pakan), C (dosis enzim papain 1,70%/kg pakan), dan D (dosis enzim papain 3,40%/kg pakan). Hasil penelitian menunjukkan bahwa penambahan enzim papain pada pakan berpengaruh nyata terhadap EPP, PER, dan RGR (Sig.<0,05). Perlakuan penambahan dosis enzim papain terbaik adalah perlakuan D (dosis enzim papain 3,40%/kg pakan) yang menghasilkan nilai EPP sebesar 59,12Â±1,74%, PER sebesar 1,74Â±0,05%, dan RGR sebesar 1,59Â±0,10%/hari. Kualitas air pada media pemeliharaan terdapat pada kisaran yang layak untuk pemeliharaan ikan uji.Â Freshwater eel (Anguilla bicolor) is a type of fish that has slow growth due to low digestibility. One effort that can be done to improve the digestion of feed is by the addition of exogenous enzymes into the eel feed. Exogenous enzyme that are often added to the feed is papain enzymes. The addition of papain enzyme into commercial feed is expected to optimize freshwater eelâ€™s digestion through its proteolytic activities. The purpose of this study is to know the effect and the best dose of papain enzyme addition into commercial feed on feed efficiency, growth rate and survival rate of freshwater eel (A. bicolor). The test fish used in this study is elver freshwater eelÂ as much as 120 fish with average weight 6,05Â±1,17 g/fish. As many as 120 aquariums with a volume of 70 liters is used as a culture container in this study. The study was conducted by experimental method of completely randomized design with one factor that is the different dose of papain enzyme which is repeated three times. The treatment used were A (dose of enzyme papain 0%/kg feed), B (dose of enzyme papain 0,85%/kg feed), C (dose of enzyme papain 1,70%/kg feed), and D (dose of enzyme papain 3,40%/kg feed). The study result showed that the addition enzyme papain in feed had significantly effect on EPP, PER and RGR (Sig.<0,05). The best treatment is treatment D (dose of papain enzyme 3,40%/kg feed) which is produce EPP values of 59,12Â±1,74%, PER of 1,74Â±0,05%, and RGR of 1,59Â±0,10%/day. Water quality in media is within a reasonable range for the culture of test fish.

Pengaruh Kepadatan yang Berbeda Terhadap Kelulushidupan Dan Pertumbuhan Ikan Nila (Oreochromis niloticus) Pada Sistem Resirkulasi Dengan Filter Arang Nugroho, Arif; Arini, Endang; Elfitasari, Tita
Journal of Aquaculture Management and Technology Vol 2, No 3 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Tilapia fish is a commodity freshwater economical. Tilapia relatively easily domesticated and have some lead among the rapid growth, high tolerance for the environment, and resistant to diseases. Fishery cultivation intensive use and dosage dense the weft of which is too high, impacts to decreasing growth and survival rate as well as the water quality. One of the efforts to overcome the problem is with the improvement of the agricultural system through by using charcoal filter in recirculation system. The study was conducted to determine the effect of different densities by using a charcoal filter in the recirculation system on the growth andsurvival rate of tilapia (Oreochromis niloticus). This study was conducted during 40 days of observation, starting from July to October 2012 at the Laboratory of Aquaculture, Diponegoro University, Semarang. Fish sample used is tilapia seed (Oreochromis niloticus) with average zise 4-6 cm. Methods used in this research is completely randomized design (CRD) with 3 treatment and 3 replication. Different dense stocking on every treatment A, B and C, are respectively 10, 15, and 20 tilapia/10 liters which are obsorved in the recirculation system using charcoal filter. The trial diets given with â€˜at satiationâ€™ methode 08.00, 12.00, and 16.00 WIB. The result showed density does not affect (P0,05) growth. The used of charcoal filter as a very effective, showed by the low concentration of ammonia in the density 20 tilapia/10 liters until 40 days of observation.

PENGARUH BERBAGAI RASIO E/P PAKAN BERKADAR PROTEIN 30% TERHADAP EFISIENSI PEMANFAATAN PAKAN DAN PERTUMBUHAN IKAN MAS (Cyprinus carpio) Pratama, Mahendra Adi; Subandiyono, -; Pinandoyo, -
Journal of Aquaculture Management and Technology Volume 4, Nomor 4, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Pakan yang baik adalah pakan yang mengandung nutrisi yang lengkap sesuai kebutuhan ikan dengan energi total yang mencukupi kebutuhan hidup ikan dan pertumbuhan ikan. Protein merupakan salah satu komponen nutrisi yang digunakan untuk sumber energi pertumbuhan. Pengaturan rasio energi protein sangat diperlukan agar dapat memaksimalkan peran protein dalam menunjang pertumbuhan benih ikan mas (Cyprinus carpio), sehingga protein tidak digunakan sebagai sumber utama energi dalam kegiatan metabolisme ikan sehari-hari. Penelitian ini bertujuan untuk mengkaji pengaruh pada perbedaan rasio energi proteinÂ dalam pakan terhadap pemanfaatan pakan dan pertumbuhan ikan mas (C. carpio). Metode penelitian yang dilakukan adalah metode eksperimental dengan rancangan acak lengkap (RAL) yang terdiri dari 4 perlakuan dan 3 kali ulangan.Â Perlakuan yang diterapkan adalah perlakuan A, B, C, dan D (E/P 8,6 kkal/g Prot; 8,8 kkal/g Prot; 9,0 kkal/g Prot; dan 9,2 kkal/g Prot). Hewan uji yang digunakan adalah ikan mas (C. carpio) dengan padat penebaran 1 ekor/L air yang ditampung dalam toples, dengan masa pemeliharaan selama 35 hari. Kualitas air pada wadah pemeliharaan ikan mas dalam kondisi optimal,dengan sistem semi resirkulasi air tertutup. Hasil penelitian menunjukan bahwa perbedaan rasio energi/protein dalam pakan dengan protein 30% pada tiap perlakuan, memberikan perbedaan nyata (P<0,05) terhadap laju pertumbuhan relatif (RGR), efisiensi pemanfaatan pakan (EPP) dan protein efisiensi rasio (PER) pada ikan mas (C. carpio).Â Pada perlakuan D merupakan hasil terbaik dengan nilai RGR (3,13 Â±0,17 %/hari), EPP (57,63Â±2,45 %), PER (1,92Â±0,08 %), TKP (28.40Â± 0.36 g) dan SR (95.83Â±0,72%).Â Perbedaan rasio energi protein pada pakan buatan memberikan pengaruh yang nyata (P<0,05) terhadap efisiensi pemanfaatan pakan, protein efisiensi rasio, dan laju pertumbuhan relatif pada benih ikan mas (C. carpio).Â A good feed contained complete nutrition according to the fish need with an adequate total energy for the fish growth. Protein was one of the nutrient component that used for the fish growth. Energy protein ratio was useful for maximize the role of protein in supporting the growth of the seed carp (Cyprinus carpio), so that the protein was not used as the main energy in the daily metabolism activity of fish. This research aim to assess the influence of by the difference in the energy protein ratio inÂ feed on the utilization of fodder and the growth of common carp (C. carpio)The research method is experimental method with completely randomized design (RAL), which consists of 4 treatments and 3 repetitions. The treatment used is treatment A, B, C, and D (E/P 8.6 Kcal/g Prot; 8.8 kcal/g Prot; 9.0 kcal/g Prot; and 9.2 kcal/g Prot). Animal tests used were carp (C. carpio) with a stocking density 1 fish /L of water is collected in a plastic tub, with a maintenance period of 35 days. The quality of water in the container maintenance of carp in optimal condition, with a closed water circulation system. The results showed that differences in protein energy ratio in the diet with 30% protein at each treatment, providie significant differences (P<0.05) on the relative growth rate (RGR), the efficiency of feed utilization (EPP), and protein efficiency ratio (PER) in carp (C. carpio). In the treatment D is the best result with the value of RGR (3.13 Â± 0.17%/day), EPP (57.63 Â± 2.45%), PER (1.92 Â± 0.08%), TKP (28.40 Â± 0.36 g ) and SR (95.83 Â± 0.72%). The difference in energy protein ratio on artificial feed provides real impact (P<0,05) against utilization efficiency of feed, protein efficiency ratio, and growth rate relative to the seed carp (C. carpio).

PATOGENISITAS ISOLAT K14 YANG DIISOLASI DARI LELE DUMBO (Clarias gariepinus) YANG BERASAL DARI DEMAK Wulandari, Ayu; Prayitno, Slamet Budi; Sarjito, -
Journal of Aquaculture Management and Technology Vol 3. No 2 (2014): Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Budidaya lele (Clarias gariepinus) berkembang pesat dan memiliki nilai ekonomis yang tinggi. Ikan lele dapat dibudidayakan di lahan dan sumber air tebatas serta padat penebaran tinggi.Â Pertumbuhan ikan lele cepat dan pemasaran mudah. Penelitian ini bertujuan untuk mengetahui tingkat patogenisitas dan leukosit total ikan lele dumbo (C. gariepinus) yang telah diinfeksi Isolat bakteri K14 dengan kepadatan yang berbeda serta identifikasi Isolat bakteri K14 pada lele dumbo secara biomolekuler berbasis PCR 16S rDNA. Penelitian ini menggunakan metode eksperimental. Ikan uji yang digunakan adalah ikan lele dumbo sebanyak 210 ekor dengan ukuran 8.02 Â± 0.6 cm Kepadatan Isolat bakteri K14 yang digunakan dalam penelitian adalah perlakuan (A) 100 CFU/mL, perlakuan (B) 104 CFU/ml, perlakuan (C) 105 CFU/ml, perlakuan (D) 106 CFU/ml, perlakuan (E) 107 CFU/ml, perlakuan (F) 108 CFU/ml, dan perlakuan (G) 109 CFU/ml. Pengamatan gejala klinis dilakukan selama 96 jam dan pengamatan leukosit total dilakukan setiap 24 jam sekali selama 6 hari. Hasil penelusuran sequen 16S rDNA mengunakan internet melalui sistem BLAST. Hasil pengamatan gejala klinis lele dumbo yang diinfeksi Isolat bakteri K14 adalah ikan berenang lemah di dasar air, berenang vertikal, nafsu makan berkurang, kulit mengelupas, haemorragic pada tubuh, kemudian timbulnya radang kemudian menjadi luka yang terbuka (ulcer). Kematian ikan tertinggi terlihat pada perlakuan F (108 CFU/mL) dan G (109 CFU/mL) yaitu 30 ekor, kemudian perlakuan E (107 CFU/mL) sebesar 24 ekor, perlakuan D (106 CFU/mL) sebesar 23 ekor, perlakuan C (105 CFU/mL) sebesar 22 ekor, dan kematian ikan paling sedikit dialami pada perlakuan B (104 CFU/mL) sebesar 2 ekor. Nilai LD50 dalam uji patogenisitas menunjukkan bahwa konsentrasi bakteri yang dapat mematikan 50% populasi ikan lele dumbo dalam waktu 96 jam adalah 4.977x105 CFU/ml. Jumlah leukosit total tertinggi setelah 24 jam penyuntikan Isolat bakteri K14 pada perlakuan G adalah 3.41x104 sel/mm3 dan terendah pada perlakuan A sebanyak 1.57x104 sel/mm3. Hasil penelusuran berdasarkan 16S rDNA menggunakan sistem BLAST diperoleh bahwa homogenitas sebesar 96% terhadap Aeromonas sp.Â Catfish farming have high value and high demand because a catfish can growt in limited water resources with high density. Catfish growth fast and easy to sell them. The purpose of this research was to determine the level of pathogenicity of K14 isolate to catfish (C. gariepinus that have inframoscularly nipelia misl K14 isolateat different densities and then biomolecularly identified based on PCR 16S Rdna. This research used experimental method. The fish sample was used catfish with 8.02 Â± 0.6 cm leght. The density of bacteria used in this research were (A) PBS, (B) 104 CFU/mL, (C) 105 CFU/mL, (D) 106 CFU/mL, (E) 107 CFU/mL, (F) 108 CFU/mL, (G) 109 CFU/mL. The observation of clinical signs carried out for 96 hours and total leukocytes were observed every 24 hours for 6 days. 16S rDNAÂ sequences of K14 isolate was casuistry the BLAST system. The clinical sign of catfish that infected by K14 isolates obtained that fish showed swimming weakly, found till vertically, poor appetite, skin peeling, haemoragic then followed by the inflammation and ulcer. The highest fish mortality was seen in the treatments F (108 CFU/mL) and G(109 CFU/mL) by 30 animals, E (107 CFU/mL) treatments 24 animal, D treatmentÂ 106 CFU/mL was valued 23 animals, C treatment 105 CFU/mL was 22 animals and B treatment 104 CFU/mL was 2 animals respectively. LD50 value of K14 demonstrated that the concentration of 105 CFU/mL has able to kill 50% of treated catfish within 96 hours. The highest total leukocytes after 24 hours injection of K14 isolate was found in the G treatment valued 3.41 x 104 cells/mm3 and the lowest found in A treatment at 1.57 x 104 cells/mm3. Reidentification of K 14 isolate using moleculer approach 16S rDNA then presented the sequence againt existing gen bank by BLAST system indicated that genetically the K14 isolate closes 96% to Aeromonas sp.

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Kota semarang,

Jawa tengah

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