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Journal of Marine Research
Published by Universitas Diponegoro
ISSN : -     EISSN : 24077690     DOI : 10.14710/jmr.v9i4.28340
Core Subject : Science,
Earth & Planetary Sciences
The Journal of Tropical Fisheries Management is managed by the Department of Water Resource Management, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University aims to publish the results of basic, applied research in the scope of fisheries resources, fish stock studies, and population dynamics, fish biodiversity, fisheries technology, industrialization and fish trade, fisheries management, and fisheries development policies in the tropics, especially Indonesia. The scope of the area includes: Marine Fisheries Coastal Fisheries Inland Fisheries The focus and scope of this publication are expected to contribute thoughts for the government to strengthen the science of fisheries management
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Articles 687 Documents
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Peningkatan Pertumbuhan Artemia sp. melalui Aplikasi Ekstrak Ulva sp. Natasya Erdza Aulia; Ervia Yudiati; Retno Hartati
Journal of Marine Research Vol 12, No 2 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i2.34629

Abstract

Kandungan gizi pada Artemia sp. merupakan salah satu faktor yang mempengaruhi penggunaannya Artemia sp. sebagai pakan alami pada budidaya biota laut. Ulva sp. memiliki potensi sebagai pakan Artemia sp. karena memiliki kandungan protein dan karbohidrat yang tinggi dan keberadaannya melimpah di Indonesia. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak Ulva sp. terhadap pertumbuhan Artemia sp. Metode penelitian yang digunakan adalah eksperimental menggunakan rancangan acak lengkap dengan berbagai konsentrai ekstrak Ulva sp. sebanyak 3 pengulangan. Laju pertumbuhan spesifik (SGR) dan laju pertumbuhan (GR) diukur setiap hari selama 5 hari, dan nilai toksisitas (LC50) menggunakan metode BSLT pada 24 jam dengan mengaplikasikan ekstrak Ulva sp. kepada Artemia sp. Hasil uji toksisitas dengan nilai LC50 sebesar 59603.40 ppm yang menunjukkan ekstrak Ulva sp. tidak toksik. Hasil penelitian menunjukkan bahwa Artemia sp. yang dipelihara dengan pemberian ekstrak Ulva sp. konsentrasi 1500 ppm mempunyai nilai laju pertumbuhan spesifik (SGR) tertinggi (29.15%) dan laju pertumbuhan (GR) tertinggi (329.58 um/day). ANOVA test menunjukkan pengaruh pada perbedaan konsentrasi Ulva sp. terhadap pertumbuhan Artemia sp. yang berbeda nyata. Penelitian ini menghasilkan pemberian ekstrak Ulva sp. mempengaruhi peningkatan pertumbuhan Artemia sp. Pertumbuhan Artemia sp. yang dipelihara dengan konsentrasi 1500 ppm memiliki nilai pertumbuhan paling tinggi. The nutritional content of Artemia sp. is one of the factors that influence the use of Artemia sp. as natural food for marine biota cultivation. Ulva sp. has potential as feed for Artemia sp. because it has a high protein and carbohydrate content and is abundant in Indonesia. This study aims to determine the effect of Ulva sp. on the growth of the toxicity of Artemia sp. The research method used is experimental using a completely randomized design using Ulva sp. extract with different concentration in 3 repetitions. Specific growth rate (SGR) and growth rate (GR) were measured daily for 5 days, and toxicity value (LC50) using the BSLT method at 24 hours by applying extracts of Ulva sp. to Artemia sp. The results of the toxicity test with an LC50 value of 59603.40 ppm which shows the extract of Ulva sp. is not toxic. The results showed that Artemia sp. which were maintained by administration of Ulva sp. concentration of 1500 ppm had the highest specific growth rate (SGR) (29.15%) and the highest growth rate (GR) (329.58 um/day). ANOVA test showed the effect on differences in concentrations of Ulva sp. on the growth of Artemia sp. significantly different. This research resulted in the administration of Ulva sp. affect the growth of Artemia sp. Growth of Artemia sp. reared with a concentration of 1500 ppm had the highest growth value.
Sebaran Mikroplastik di Pantai Sepanjang, Kabupaten Gunungkidul Dea Nebraska Norindra; Heny Budi Setyorini; Sri Haryanti Prasetiyowati
Journal of Marine Research Vol 12, No 2 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i2.37442

Abstract

Pantai Sepanjang di Kabupaten Gunungkidul memiliki kegiatan pariwisata yang menyebabkan produksi sampah plastik. Mikroplastik akan berdampak buruk bagi biota, manusia, dan lingkungan. Tujuan dari penelitian ini adalah mengetahui jenis, jumlah dan sebaran mikroplastik di Pantai Sepanjang. Metode dalam penelitian ini adalah observasi atau survei lapangan. Lokasi penelitian ditentukan secara purposive sampling sebanyak 9 titik sampling. Sampel sedimen diambil menggunakan pipa paralon 4inch pada kedalaman 10-15 cm. Tahap pengumpulan data meliputi pengeringan, pemisahan sedimen, pembuatan supernatan, penyaringan dan identifikasi jenis mikroplastik menggunakan mikroskop binokuler dengan perbesaran 10x. Analisis data dilakukan secara statistik dan spasial. Analisis statistik menggunakan uji regresi dan korelasi antara jarak pengambilan sampel dan jumlah mikroplastik. Analisis spasial menggunakan software GIS untuk menghasilkan peta sebaran mikroplastik. Hasil penelitian menunjukkan bahwa terdapat 4 jenis mikroplastik di Pantai Sepanjang, antara lain fiber, film, fragmen, dan pelet. Jumlah mikroplastik tertinggi adalah jenis fiber sebanyak 60 partikel/gr di titik C. Hasil analisis regresi dan korelasi tertinggi terdapat pada jenis fragmen dengan nilai R2 = 0,27, yang menunjukkan hubungan antara jarak pengambilan sampel dan jumlah mikroplastik lemah. Hasil analisis spasial menunjukkan bahwa sebaran mikroplastik di Pantai Sepanjang dominan ke arah barat, dan semakin ke arah barat maka jenis mikroplastik semakin beragam. Sepanjang Beach at Gunungkidul District have tourism activities that led to plastic waste production. The microplastic will be considered harmful to biota, human, and environment. This study aims to identify the various types, numbers and distributions of microplastic at Sepanjang Beach. The method used in the study was a survey method. The research locations were determined by site survey using purposive sampling with 9 sample points. The sediment sample collection was conducted by using the PVC pipes 4inch in depth of 10-15 cm. The data collection step is drying, sediment separation, making of the supernatant, filtration, and identification using a binocular microscope with the 10x zoom. The analysis of data was conducted statistically and spatially. The statistical analysis was done by using the regression and correlation analysis between sampling distance and number of microplastics. The spatial analysis was done by using GIS software to create the map of microplastic distribution. The result showed that 4 types of microplastics at Sepanjang Beach were fiber, films, fragments, and pellets. The highest number of microplastics were fiber with 60 particles/gram in the sampling location of C. The highest of regression and correlation analysis were fragments with the value of R2 = 0,27, that indicated the weak correlation between sampling distance and number of microplastics. The spatial analysis showed that distribution of microplastics at Sepanjang Beach were dominant to the west, and the further to the west area, the type of microplastics were more diverse.
Pemodelan Perubahan Dasar Perairan (Bed Level Change) di Pantai Moro, Kabupaten Kendal Muhammad Chiesa Fathirayan; Hariyadi Hariyadi; Alfi Satriadi
Journal of Marine Research Vol 12, No 2 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i2.31252

Abstract

Pantai Moro, Gubugsari, merupakan salah satu daerah wisata yang potensial di Kabupaten Kendal. Pantai Moro memiliki menara pandang yang menjadi ciri khas. Faktor alam berupa perubahan dasar perairan di Pantai Moro perlu diperhatikan untuk menunjang kegiatan masyarakat. Tujuan dari penelitian ini adalah untuk mengetahui sebaran jenis sedimen dan perubahan dasar perairan berdasarkan hasil model. Pemodelan untuk mengetahui perubahan dasar perairan dilakukan menggunakan perangkat lunak MIKE21 modul Sand Transport dengan memasukkan data-data seperti batimetri, pasang surut, arus, gelombang, angin dan karakteristik sedimen dasar. Pemodelan dilakukan selama satu bulan setiap musim. Sedimen dasar di Perairan Pantai Moro berupa pasir, lanau, pasir lanauan dan lanau pasiran pada daerah pantai dan lanau pada daerah laut. Hasil pemodelan menunjukkan terjadi sedimentasi pada daerah dekat pantai sebesar 0,0945 m - 0,0005 m, dan terjadi erosi pada daerah muara sebesar 0,005 m.   Moro Beach, Gubugsari District is one of potential tourism areas in Kendal Regency. Moro Beach has sight tower, which is the hallmark of the area. Natural factor in the form of bed level changes is need to be considered to support community activities. The purpose of this study was to determine the distribution of sediment types and the bed level change of Moro Beach based on model result. The model is using MIKE 21 software ; Sand Transport (ST) module with input data of bathimetry, tides, current, waves, wind and seabed sediment. Sediment of Moro Beach is sand, silt, sandy silt and silty sand in beach area and silt in sea area. The result of modeling is indicates the sedimentation process near the beach with a value of 0,0945 m - 0,0005 m and erosion process in the estuary area with a value of 0,005 m.
Fenomena Infeksi Acute Hepatopancreatic Necrosis Disease pada Budidaya Udang Vaname di Kabupaten Bangkalan Amirul Suryana; Eka Nurrahema Ning Asih; Insafitri Insafitri
Journal of Marine Research Vol 12, No 2 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i2.35632

Abstract

Acute hepatopancreatic necrosis disease (AHPND) merupakan salah satu penyakit yang menginfeksi udang vaname (Litopenaeus vannamei) dengan tanda berupa udang lesu, hepatopankreas mengalami fenomena nekrosis, atrofi dan terlihat pucat. Infeksi penyakit ini minim publikasi, sehingga perlu dilakukan penelitian awal untuk menginspeksi keberadaan penyakit ini di udang vaname di Indonesia khususnya di tambak udang Bangkalan. Upaya ini dilakukan sebagai langkah antisipasi agar prevelensi AHPND tidak meluas di perairan Bangkalan. Tujuan penelitian ini adalah untuk mengetahui karakteristik infeksi dari penyakit AHPND pada budidaya udang vaname di Kabupaten Bangkalan.Pengambilan sampel udang menggunakan metode purposive sampling dari tambak udang bagian selatan dan utara Bangkalan. Identifikasi AHPNDdilakukan dengan metode nested polymerase chain reaction (nested PCR) menggunakan primer AP4. Hasil diagnosis morfologis yang diduga diakibatkan oleh penyakit AHPND terdapat pada sampel 1, 2, dan 3 dengan karakteristik pertumbuhan yang lambat dan pada sampel 9 berupa kotoran yang berwarna putih. Diagnosis genetik DNA VpAHPND menunjukkan sampel 1, 2,dan 3 positif penyakit AHPND dan sesuai gejala morfologis yang di dapat. Munculnya penyakit AHPND  di duga karena ketidaksesuainsuhudan pH dengan standar baku mutu untuk pemeliharaan udang vanamei. Data penelitian ini menjadi informasi awal tentang visualisi dan ciri-ciri udang yang terinfeksi AHPND yang dapat dimanfaatkan pembudidaya udang vaname agar waspada terhadap penyakit tersebut.  Acute hepatopancreatic necrosis disease (AHPND) is a disease that infects white shrimp (Litopenaeus vannamei) with signs of lethargic shrimp, hepatopancreas experiencing necrosis, atrophy, and pale appearance. The infection of this disease is minimally published, so it is necessary to conduct preliminary research to inspect the presence of this disease in white shrimp in Indonesia, especially in Bangkalan shrimp ponds. This effort is carried out as an anticipatory step so that the prevalence of AHPND does not expand in Bangkalan waters. The purpose of this study was to determine the characteristics of infection from AHPND disease in vaname shrimp culture in Bangkalan Regency. A sampling of shrimp using the purposive sampling method from shrimp ponds in the south and north of Bangkalan. Identification of AHPND was done by nested PCR method using AP4 primer. The results of the morphological diagnosis suspected to be caused by AHPND were found in samples 1, 2, and 3 with slow growth characteristics and sample 9 in the form of white feces. Genetic diagnosis of VpAHPND DNA showed samples 1, 2, and 3 were positive for AHPND disease and according to the morphological symptoms obtained. The emergence of AHPND disease is thought to be due to the incompatibility of temperature and pH with quality standards for the maintenance of vaname shrimp. The data of this study serve as initial information about the visualization and characteristics of shrimp infected with AHPND that can be used by vaname shrimp farmers to be aware of the disease.
Antimicrobial Potential of Gorgonian Alcyonium sp. Associated Bacteria Against Human Pathogenic Skin Diseases Avicenna Wijayanto; Agus Sabdono; Endang Sri Lestari; Mada Triandala Sibero
Journal of Marine Research Vol 12, No 2 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i2.35042

Abstract

Skin diseases are common in tropical countries due to microbial pathogens. The use of antibiotics is one of the efforts to treat skin diseases. However, the misuse and overuse of antibiotics can stimulate the emergence of multi-drug resistance (MDR) pathogens. This study focused on obtaining gorgonian-associated bacteria with antimicrobial activity against Cutibacterium acnes, Staphylococcus epidermidis, Malassezia furfur, and Candida albicans. This research procedure includes sampling, isolation, and purification of bacteria, antimicrobial test with agar plug method, morphological characterization, and molecular identification. The study results showed that one isolate of gorgonian-associated bacteria Alcyonium sp. from Seruni Island, Karimunjawa has antimicrobial activity against Cutibacterium acnes, Staphylococcus epidermidis, and Candida albicans. The results of molecular identification showed that isolate SE.4.2 had a similarity of 98.96% with Pseudoalteromonas shioyasakiensis.
Pemangsaan Herbivori Daun Mangrove Di Kawasan Tracking Mangrove Kemujan, Kepulauan Karimunjawa Bambang Jati Laksono; Nirwani Soenardjo; Raden Ario
Journal of Marine Research Vol 12, No 2 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i2.35780

Abstract

Ekosistem mangrove merupakan kumpulan komunitas flora dan fauna di kawasan perairan bersalinitas. Ekosistem mangrove menghasilkan sebuah interaksi saling ketergantungan antara organisme dengan lingkungan. Herbivori merupakan peristiwa hilangnya komponen daun mangrove akibat di mangsa biota. Kerusakan herbivori yang tinggi mampu mempengaruhi aktivitas fotosintesis mangrove serta mempengaruhi produktivitas perairan. Penelitian ini bertujuan untuk menganalisis tingkat herbivori daun mangrove Ceriops tagal dan Rhizophora mucronata pada ekosistem mangrove di sekitar Tracking Mangrove Kemujan, Kepulauan Karimunjawa, Kabupaten Jepara, Jawa Tengah. Metode deskriptif digunakan dalam menganalisis data sedangkan metode purposive digunakan dalam penentuan lokasi dan pengambilan sampel. Sampel daun spesies Ceriops tagal dan Rhizophora mucronata diambil berdasarkan tiga kategori ketinggian <1 m, 1-2 m, dan >2 m, masing-masing sebanyak 10 pohon sebagai ulangan. Daun dipisahkan berdasarkan umur daun (muda dan tua) dan kondisi daun (rusak dan utuh). Total daun yang diambil sebanyak 10% dari setiap pohon. Hasil penelinitan menunjukkan nilai rata-rata tingkat herbivori pada setiap kategori spesies, umur daun dan tinggi pohon yaitu pada spesies Ceriops tagal 7,66% kisaran 0,01-39,05 hingga 10,93% kisaran 0,2-55,46 sedangkan pada spesies Rhizophora mucronata sebesar 5,52% kisaran 0,01-11,13 hingga 7,73% kisaran 0,01-39,05. Tingkat herbivori C. tagal lebih tinggi daripada R. mucronata. Rendahnya persentase tingkat herbivori mengidentifikasikan bahwa ekosistem mangrove Kemujan masih tergolong sehat. The mangrove ecosystem is a collection of flora and fauna communities in saline waters. The mangrove ecosystem produces an interdependent interaction between organisms and the environment. Herbivory is the loss of mangrove leaf components due to being eaten by biota. High damage to herbivores can affect the photosynthetic activity of mangroves and affect water productivity. The purpose of this study was to analyze the herbivory level of mangrove leaves of Ceriops tagal and Rhizophora mucronata in the mangrove ecosystem around the Kemujan Mangrove Tracking, Karimunjawa Islands. Descriptive method is used in analyzing the data while purposive method is used in determining the location and taking samples. Leaf samples of Ceriops tagal and Rhizophora mucronata species were taken based on three different height categories, namely <1 m, 1-2 m, and> 2 m, each with 10 trees as replicates. The leaves are separated based on the age of the leaves (young and old) and the condition of the leaves (damaged and intact). Total leaves taken as much as 10% of each tree.The results showed an average value of herbivory levels in each species category, age of leaves and height of the tree, namely the species Ceriops tagal of 7.73% range 0.18-30.38 to 10.93% range 0.2- 55,46 while in Rhizophora mucranata species the range is 5.52% 0.01-11.13 to 7.73% range 0.01-39.05. The herbivory level of C. tagal is higher than that of R. mucronata. The low percentage of herbivory indicates that the Kemujan mangrove ecosystem is still relatively healthy.
Estimasi Serapan Karbon Lamun di Pulau Kelapa Dua, Taman Nasional Kepulauan Seribu Josua Kristanto Pandiangan; Munasik Munasik; Ita Riniatsih
Journal of Marine Research Vol 12, No 2 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i2.35572

Abstract

Ekosistem pesisir dan lautan memiliki peran penting dalam mengontrol siklus karbon karena kemampuannya menyerap serta menyimpan karbon dalam jumlah besar dan dalam jangka waktu panjang. Padang lamun merupakan salah satu ekosistem pesisir yang memiliki peran penting sebagai carbon sink di laut. Penelitian ini bertujuan untuk mengetahui kandungan simpanan karbon, jumlah spesies/jenis lamun, nilai persen cover dan tegakan lamun yang ada di Pulau Kelapa Dua, Kepulauan Seribu. Pengambilan data persentase tutupan lamun dilakukan dengan metoda line transek kuadran yang mengacu pada metode LIPI 2017 dan penentuan lokasi dipilih dengan menggunakan metode purposive sampling. Pengambilan data lamun dalam penelitian ini dilakukan pada lokasi penelitian, Pengambilan data dilakukan untuk menghitung nilai kerapatan serta persentase penutupan lamun yang dilakukan pada titik yang telah di tentukan. Pengambilan sampel biomassa lamun dilakukan pada titik 0m, 50m dan 100m menggunakan sekop pada daerah di luar transek kuadran, pada cuplikan kuadran seluas 20x20cm. Kandungan karbon dihitung dengan metode Loss on Ignition (LOI), kemudian dikonversikan dengan nilai biomassa setiap titiknya. Jenis lamun yang ditemukan pada ketiga stasiun terdapat 4 spesies yaitu Cymodocea rotundata, Thalassia hemprichii, Halophila ovalis dan Halodule pinifolia. Cymodocea rotundata mendominasi pada stasiun pertama dan kedua dengan kerapatan mencapai 301 tegakan/m2 pada stasiun 1 dan 881 tegakan/m2 pada stasiun 2, sedangkan pada stasiun 3 didominasi oleh Thalassia hemprichii dengan kerapatan mencapai 559 tegakan/m2. Nilai biomassa dibawah substrat lebih besar dibandingkan di atas substrat (131,96 gbk/m2 dan 78,43 gbk/m2). Total kandungan karbon pada Pulau Kelapa Dua, Kepulauan Seribu adalah 39,96 gC/m2. Kandungan karbon lebih banyak tersimpan di jaringan lamun bawah substrat (akar dan rhizoma) pada spesies Thalassia hemprichii. Coastal and oceanic ecosystems have an important role in controlling the carbon cycle because of their ability to absorb and store large amounts of carbon over a long period of time. Seagrass beds are one of the coastal ecosystems that have an important role as a carbon sink in the sea. This study aims to determine the content of carbon storage, the number of species/types of seagrass, the value of percent cover and seagrass stands in Kelapa Dua Island, Seribu Islands. Data collection on the percentage of seagrass cover was carried out using the quadrant line transect method which refers to the 2017 LIPI method and the determination of the location was selected using the purposive sampling method. Seagrass data collection in this study was carried out at the research location. Data collection was carried out to calculate the density value and the percentage of seagrass cover carried out at a predetermined point. Sampling of seagrass biomass was carried out at 0m, 50m and 100m using a shovel in the area outside the quadrant transect, on a quadrant sample of 20x20cm. Carbon content is calculated by the Loss on Ignition (LOI) method, then converted to the biomass value at each point. There were 4 species of seagrass found at the three stations, namely Cymodocea rotundata, Thalassia hemprichii, Halophila ovalis and Halodule pinifolia. Cymodocea rotundata dominated at the first and second stations with a density reaching 301 stands/m2 at station 1 and 881 stands/m2 at station 2, while at station 3 it was dominated by Thalassia hemprichii with a density reaching 559 stands/m2. The biomass value under the substrate was greater than that above the substrate (131.96 gbk/m2 and 78.43 gbk/m2). The total carbon content at the Kelapa Dua Island, Kepulauan Seribu is 39.96 gC/m2. More carbon content is stored in the seagrass tissue under the substrate (roots and rhizomes) in Thalassia hemprichii species.
Potensi Simpanan Karbon Padang Lamun di Pulau Sintok dan Menjangan Kecil, Kepulauan Karimunjawa Marthin Ricky Sipayung; Ita Riniatsih; Subagiyo Subagiyo
Journal of Marine Research Vol 12, No 2 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i2.34175

Abstract

Karbon dioksida (CO2) memiliki kontribusi yang paling tinggi terhadap kandungan Gas Rumah Kaca yaitu sebesar 55% dari emisi karbon oleh aktivitas manusia. Fenomena ini menyebabkan perubahan iklim yang berdampak pada meningkatnya suhu ekstrim, banjir, topan, badai, kekeringan dan naiknya permukaan laut hingga makhluk hidup merasakan dampak negatif langsung dari pemanasan global. Padang lamun adalah vegetasi yang memiliki potensi besar dalam mengurangi dampak emisi di lingkungan dengan menyerap CO2 dan menghasilkan O2. Penelitian ini bertujuan untuk mengetahui estimasi simpanan karbon, jumlah spesies/jenis lamun, nilai persen cover dan tegakan lamun yang ada di Pulau Sintok dan Pulau Menjangan Kecil, Kepulauan Karimunjawa. Penelitian ini menggunakan metode survei dan penentuan lokasi dipilih dengan menggunakan metode purposive sampling, sedangkan metode pengambilan data lamun melalui metode line transect quadrant. Hasil pada penelitian ini ditemukan 4 jenis lamun, yaitu Cymodocea rotundata, Thalassia hemprichii, Halophila ovalis dan Enhalus acoroides. Cymodocea rotundata mendominasi dikedua lokasi dengan kerapatan mencapai 3156 ind/m2 pada lokasi Pulau Sintok, dan 828 ind/m2 pada lokasi Pulau Menjangan Kecil. Nilai biomassa dibawah substrat (akar dan rhizoma) dan diatas substrat (daun) pada Lokasi I (223,99 gbk/m2 dan 199,02 gbk/m2) menunjukkan nilai yang lebih kecil dibandingkan nilai biomassa dibawah substrat dan diatas substrat pada Lokasi II (474,48 gbk/m2 dan 269,64 gbk/m2). Total kandungan karbon pada lokasi Pulau Sintok, Karimunjawa adalah 26,09 gC/m2 dan pada lokasi Pulau Menjangan Kecil, Karimunjawa adalah 74,15 gC/m2. Kandungan karbon lebih banyak tersimpan di jaringan lamun bawah substrat (akar dan rhizoma) dengan spesies Enhalus acoroides.   Carbon dioxide (CO2) has the highest contribution to the Greenhouse Gas content, which is 55% of carbon emissions by human activities. This phenomenon causes climate change which has an impact on increasing extreme temperatures, floods, typhoons, storms, droughts and rising sea levels so that living things feel the direct negative impact of global warming. Seagrass beds are vegetation that has great potential in reducing the impact of emissions on the environment by absorbing CO2 and producing O2. This study aims to determine the estimated carbon storage, the number of species/types of seagrass, the value of percent cover and seagrass stands in Sintok Island and Menjangan Kecil Island, Karimunjawa Islands. This study used a survey method and the determination of the location was selected using the purposive sampling method, while the seagrass data collection method was through the line transect quadrant method where this method refers to the 2017 LIPI method. There were 4 species of seagrass found in both locations, namely Cymodocea rotundata, Thalassia hemprichii, Halophila ovalis and Enhalus acoroides. Cymodocea rotundata dominated at both locations with densities reaching 3156 ind/m2 at the Sintok Island location, Karimunjawa and 828 ind/m2 at the Menjangan Kecil Island location, Karimunjawa. The value of biomass below the substrate and above the substrate at Location I (75,66 gbk/m2 and 34,03 gbk/m2) showed a smaller value than the value of biomass below the substrate and above the substrate at Location II (233,24 gbk/m2 and 121,63 gbk/m2). The total carbon content at the location of Sintok Island, Karimunjawa is 26,09 gC/m2 and at the location of Menjangan Kecil Island, Karimunjawa is 74,15 gC/m2. Most of the carbon content is stored in the seagrass tissue under the substrate (roots and rhizomes) with of Enhalus acoroides. 
Potensi Bakteri Halofilik Ekstrim dari Tambak Garam Tradisional sebagai Penghambat Aktivitas Bakteri Salmonella sp. Eka Nurrahema Ning Asih; Dewi Anugrah Fitri; Ary Giri Dwi Kartika; Sri Astutik; Makhfud Efendy
Journal of Marine Research Vol 12, No 3 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i3.35372

Abstract

Infeksi bakteri Salmonella sp. di perairan dapat merugikan perikanan budidaya, menurunkan kualitas hasil perikanan tangkap dan merusak kualitas produk olahan perikanan. Perlu dilakukan penelitian untuk mengatasi dan mencegah aktivitas bakteri Salmonella sp. pada beberapa sektor perikanan tersebut. Salah satu upaya yang bisa dilakukan adalah memanfaatkan sumberdaya bakteri halofilik yang hidup melimpah di air meja kristalisasi tambak garam tradisional sebagai agen penghambat aktivitas bakteri Salmonella sp.. Penelitian ini bertujuan untuk mengetahui potensi bakteri halofilik ekstrim yang dikultur dari tambak garam tradisional Pamekasan-Madura sebagai penghambat aktivitas bakteri Salmonella sp.. Metode yang digunakan dalam penelitian terdiri dari beberapa tahap yaitu isolasi, purifikasi, uji pewarnaan gram dan uji zona hambat bakteri. Karakteristik morfologi dan pengelompokan gram bakteri halofilik ekstrim diamati dibawah mikroskop binokuler model CX43RF dengan tipe kamera digital MDCE-5C. Uji aktivitas isolat bakteri halofilik ekstrim terhadap Salmonella sp. dilakukan dengan uji zona hambat menggunakan metode overlay dan difusi. Hasil penelitian diperoleh menunjukkan bahwa isolat bakteri halofilik esktrim mampu menghambat aktivitas Salmonella sp. dengan kisaran zona hambat berdiameter 4,28 mm dan 2,45 mm. Hasil penelitian ini diharapkan menjadi informasi awal untuk dipertimbangkan dalam pengembangan pemanfaatan bakteri halofilik ekstrim di bidang bioteknologi khususnya farmakologi laut kedepannya. Salmonella sp. in the waters can be detrimental to aquaculture, reduce the quality of captured fishery products and damage the quality of processed fishery products. Research needs to be done to overcome and prevent the activity of Salmonella sp. in some of the fisheries sectors. One of the efforts that can be done is to utilize the halophilic bacteria resources that live abundantly in the crystallization table water of traditional salt ponds as agents that inhibit the activity of these bacteria. This study aims to determine the potential of extremely halophilic bacteria cultured from Pamekasan-Madura traditional salt ponds as inhibitors of Salmonella sp. bacteria activity. The method used in this study consisted of several stages, namely isolation, purification, gram staining test, and bacterial inhibition zone test. Morphological characteristics and gram grouping of extremely halophilic bacteria were observed under a CX43RF binocular microscope with a digital camera type MDCE-5C. Activity test of extremely halophilic bacteria isolates against Salmonella sp. was carried out by testing the zone of inhibition using overlay and diffusion methods. The results showed that the extreme halophilic bacteria isolates were able to inhibit the activity of Salmonella sp. with a range of 4.28 mm and 2.45 mm diameter inhibition zones. The results of this study are expected to be initial information to be considered in the development of the use of extremely halophilic bacteria in the field of biotechnology, especially marine pharmacology in the future.
Konsentrasi Benzalkonium Klorida Di Perairan Segara Anakan, Cilacap Nurina Ayu; Dewi Kresnasari
Journal of Marine Research Vol 12, No 3 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i3.40387

Abstract

Benzalkonium klorida (BAC) mengalami peningkatan penggunaan setelah pandemi Covid-19, namun penggunaan senyawa ini belum teregulasi secara intensif sehingga eksistensinya di perairan umum berpotensi menjadi ancaman serius bagi ekosistem. Penelitian ini bertujuan mengkaji konsentrasi BAC di Segara Anakan sebagai salah satu ekosistem yang mendapat masukan dari aliran-aliran sungai di mana terdapat banyak aktivitas manusia. Pengambilan sampel air dilakukan menggunakan metode purposive random sampling pada lima lokasi di Segara Anakan dengan kriteria yaitu: lokasi yang dekat dengan aktivitas domestik penduduk, lokasi yang jauh dari aktivitas domestik penduduk (termasuk yang dikelilingi oleh hutan mangrove), lokasi di sekitar kawasan industri, serta lokasi dengan limpasan air tawar atau muara sungai. Kisaran BAC yang ditemukan pada kolom air permukaan Segara Anakan dari Stasiun 1 hingga 5 adalah 0,139 µg/l, 0,235 µg/l, 0,189 µg/l, 0,206 µg/l, dan 0,245 µg/l. Sedangkan konsentrasi pada kolom air di permukaan dasar dari Stasiun 1 hingga 5 adalah 0,126 µg/l, 0,222 µg/l, 0,196 µg/l, 0,175 µg/l, dan 0,241 µg/l. Hasil penelitian menunjukkan bahwa BAC merupakan mikropolutan yang ditemukan di perairan Segara Anakan Cilacap. Konsentrasi BAC yang ditemukan juga dipengaruhi oleh nilai parameter fisikokimiawi perairan.  The use of benzalkonium chloride (BAC) has been increasing after the Covid-19 pandemic, but there are still no intensive regulation about the utilization of of this compound so that its presence in public waters has the potential to become a serious threat to ecosystems. This study aimed to examine the concentration of BAC in Segara Anakan as an ecosystem that receives input from rivers contaminated by domestic waste as a result of human activities. Water samples were taken using a purposive random sampling method at five locations in Segara Anakan with the following criteria: locations close to the domestic activities, locations far from domestic activities (including those surrounded by mangrove forests), locations around industrial areas, and locations with fresh water or river estuary runoff. The BAC concentration in the Segara Anakan surface water column from Stations 1 to 5 were 0.139 µg/l, 0.235 µg/l, 0.189 µg/l, 0.206 µg/l, and 0.245 µg/l, respectively. Meanwhile, the concentrations in the water column at the bottom surface from Stations 1 to 5 were 0.126 µg/l, 0.222 µg/l, 0.196 µg/l, 0.175 µg/l, and 0.241 µg/l, respectively. The results showed that BAC was a micropollutant found in the waters of Segara Anakan Cilacap. The concentrations were also influenced by the physicochemical parameter values of the waters.

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