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Biodiversitas Journal of Biological Diversity
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Articles 6,269 Documents
Smallholder farmers livestock production on the face of climate change in Bahir Dar, Zuria District, Northwestern Ethiopia BIMREW ASMARE; FENTAHUN MEHERET
Biodiversitas Journal of Biological Diversity Vol. 19 No. 6 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190642

Abstract

Asmare B, Meheret F. 2018. Smallholder farmers livestock production on the face of climate change in Bahir Dar, Zuria District, Northwestern Ethiopia. Biodiversitas 19: 2329-2334. The objective of the study was to assess the livestock production and marketing system in smallholder systems of the area Bahir Dar Zuria district. Three kebeles were selected from Bahir Dar Zuria district based on livestock production experience and accessibility to the road. A total of 90 respondent farmers were used to conduct the assessment, and 4 key informants in each Kebele were used to get additional information. Primary data were collected using a semi-structured questionnaire and supplemented with key informants and secondary data sources. Descriptive statistics such as frequency (%) and mean were employed to present the qualitative variables obtained from the survey. The average land holding in the area was 0.73 ha of total land with 0.53 ha cropland and 0.2 ha private grazing land. With regard to labor allocation for livestock, family labor was involved in 91.18% of respondents while in the rest hired labor herded, fed, and watered livestock. With regard to water sources, the major source of water for livestock in the households was river (89%) while the remaining respondents use dug well for their livestock. The frequency of watering livestock was twice a day (51%) in the dry season and once a day (44.3%) in the wet season. Most of respondents (53.65%) sell their live animals during religious festivities followed by selling during the season of critical feed shortage (24.45%). Although smallholder farmers are vulnerable groups to climate change the majority (42%) had no information about climate change problem in the study area. The perception of household heads indicated that the livestock holding in the last five years of the family increased in most of the respondents (51%), followed by decreased condition (33%) and remained constant for the rest of the respondents (16%). Overall, it is important to assist livestock producer in enabling them benefit most from livestock and their products.
The morphological diversity of Chrysanthemum resulted from gamma ray irradiation EMI SUSILA; ARI SUSILOWATI; AHMAD YUNUS
Biodiversitas Journal of Biological Diversity Vol. 20 No. 2 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200223

Abstract

Abstract. Susila E, Susilowati A, Yunus A. 2019. The morphological diversity of Chrysanthemum resulted from gamma ray irradiation. Biodiversitas 20: 463-467. Chrysanthemum is a popular ornamental plant. The high market demand makes breeders develop new cultivars to meet market preferences. One way to get superior varieties of Chrysanthemum is by using gamma ray mutation induction which will increase the morphological variations. The purpose of this study is to determine the Chrysanthemum’s morphological diversity resulted from gamma irradiation through morphological markers. This experimental research used a completely randomized design (CRD) with one factor, i.e. radiation doses: 0 Gy (control), 10 Gy, 15 Gy, and 20 Gy. The observations were carried out qualitatively and quantitatively. The data obtained were analyzed using the SPSS 15.0 and the NTSYS (Numerical Taxonomy and Multivariate Analysis System) 2.02 program. The results showed that increasing the dose of gamma irradiation inhibited plant growth. Irradiation at 10 Gy and 20 Gy produced the most color changes in flowers compared to other doses. The basic color of 0 Gy irradiation is purple. Irradiation at 10 Gy and 20 Gy produced dark purple and deep red. Quantitative data showed that the treatment of gamma ray irradiation significantly affected the leaf length, leaf width, stem diameter, stem length, and diameter of flowers. The irradiation also caused changes in shape and texture of the leaves. The dendrogram showed that 20 Gy irradiation resulted the most diverse morphology compared to the control, 10 Gy and 15 Gy.
Characterization of phytochemicals and yield components in various okra (Abelmoschus esculentus) genotypes MUHARAMA YORA; MUHAMAD SYUKUR; SOBIR SOBIR
Biodiversitas Journal of Biological Diversity Vol. 19 No. 6 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190641

Abstract

Yora M, Syukur M, Sobir. 2018. Characterization of phytochemicals and yield components in various okra (Abelmoschus esculentus) genotypes. Biodiversitas 19: 2323-2328. Okra is a group of vegetable and medicinal plants possessing various health benefits. This plant has different shapes, colors and nutritional content. In Indonesia, this plant has not been widely cultivated, because the socialization and information of okra benefits to the Indonesian people are still limited. The aims of this study were to analyze phytochemicals, anthocyanins and carotene content, and to analyze the correlation among okra genotypes based on their morphological characters. This study was conducted from September 2017 to January 2018, using Complete Randomized Group Design with three replications and 13 genotypes namely PB01Nai, PB02Zah, PB03Clem, PB04Stri, PB13-293, PB05-291, PB06-004, PB07-127, PB08P1, PB09P2, PB10-MC, PB11-069, and PB12-OR. The observation was made on the variables of phythochemical, anthocyanin, carotene, chlorophyll and yield components on okra fruit. The results showed that all okra fruits have steroid phytochemical content and some of them had phytochemical content of Saponin and Tanin. Based on the carotene and chlorophyll analysis of 13 okra fruit genotypes, it showed that PB11-069 genotype contained the highest carotene and chlorophyll contents. While okra fruit having the highest anthocyanin content was PB05-291. The results of cluster analysis showed that okra was clustered into three color groups. The anthocyanin content found in okra fruit does not correlated with the yield component variable. Chlorophyll-?, ?, and total chlorophyll are positively correlated to carotene in okra fruit and variable number of fruits. The character of number and weight per plant greatly influences the determination of the selection process. Accordingly, the characters can be used as a selection character so as to produce a high yield okra containing high chlorophyll and carotene contents.
Mass production of the entomopathogenic nematode, Steinernema carpocapsae on Tenebrio molitor and Spodoptera litura HERI PRABOWO; SRI ADIKADARSIH; JANIS DAMAIYANI
Biodiversitas Journal of Biological Diversity Vol. 20 No. 5 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200525

Abstract

Abstract. Authors. 2019. Mass production of the entomopathogenic nematode, Steinernema carpocapsae on Tenebrio Molitor and Spodoptera litura. Biodiversitas 20: 1344-1349. Steinernema carpocapsae is the most developed biological agents to control pests nowadays, to support this nematode as a biological agent mass production technique is required. S. carpocapsae can be produced via in vivo methods. In vivo production has some advantages, such as cheap, easy, and sustainable to be developed in small farm agriculture. The purpose of this research was to evaluate the mass production of S. carpocapsae using Tenebrio molitor and Spodoptera litura larvae as hosts. Twenty-five last instar larvae were inoculated with different concentrations of infective juvenile (IJ) (e.g., 100, 200, 300 and 400 IJ/larvae) of S. carpocapsae Asembagus. The production of S. carpocapsae with concentrations of 100, 200, 300 and 400 IJ/larvae on T. molitor and S. litura showed 3.62x103; 6.29x103; 1.13x104; 1.52x104; 1.13x104; 1.46x104; 1.54x104; and 1.94x104 IJ/larvae, respectively. The IJ harvest from T. molitor and S. litura shows the longest body length for IJ at a concentration of 400 IJ/larva of 437.48µm and 591.42µm, respectively. This study indicates that T. molitor and S. litura can be used to produce S. carpocapsae. The production of S. carpocapsae with a concentration of 400 IJ / larvae on T. molitor and S. litura showed the highest total yield and body length of IJ.
Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria PRAMESITA PRAWADIKA APSARI; SRI BUDIARTI; ARIS TRI WAHYUDI
Biodiversitas Journal of Biological Diversity Vol. 20 No. 5 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200504

Abstract

Abstract. Apsari PP, Budiarti S, Wahyudi AT. 2019. Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria. Biodiversitas 20: 1259-1265. Based on the ability of actinomycetes as an antibacterial compounds producer and the need of finding novel antibacterial compounds, this study aims to look for antibacterial compounds from rhizosphere actinomycetes against bacteria in urine of UTI patients (Escherichia coli, Citrobacter braakii, Acinetobacter calcoaceticus, and Klebsiella pneumoniae). The screening of 21 actinomycetes was conducted based on the paper disc method. Potential actinomycetes that inhibited test bacteria were, then cultured in liquid medium and the supernatant was tested to six test bacteria. Then, the supernatant was extracted using ethyl acetate and crude extract from extraction process was tested to test bacteria. Afterward, the crude extract was scanned for bioactive compounds by GC-MS. Potential actinomycetes were identified by 16S rRNA gene to reveal the species. The screening results showed that ARJ 16, 24, and 36 had a wider inhibition zone than others. All of them showed that the supernatant and the crude extract could inhibit UTI's bacteria. The highest abundance of bioactive compound of crude extract was found in Propane, 1,2-dichloro, n-Hexadecanoic acid, and Carbonochloridic acid, 2-chloroethyl ester, respectively. Identification of potential actinomycetes based on 16S rRNA gene showed that ARJ 16 and ARJ 24 were highly similar to Streptomyces sp. in 99% and ARJ 36 was similar to Streptomyces tendae in 98%.
Identification of Beche-de-mers from Indonesia by molecular approach GINTUNG PATANTIS; ARIYANTI S DEWI; YUSRO N FAWZYA; MUHAMMAD NURSID
Biodiversitas Journal of Biological Diversity Vol. 20 No. 2 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200233

Abstract

Abstract. Patantis G, Dewi AS, Fawzya YN, Nursid M. 2019. Identification of Beche-de-mers from Indonesia by molecular approach. Biodiversitas 20: 537-543. Sea cucumbers have been commercially traded around the world due to their nutritional values and health benefits. Sea cucumbers are marketed as processed sea cucumber, also known as beche de mer, teripang, haisen or gamat. Due to the high global demand of beche-de-mers, there have been several cases of fraud due to the visual shape and color similarities of processed sea cucumbers. Additionally, the local name diversity of Indonesian sea cucumbers making the identification of beche-de-mers difficult. Therefore, this study aimed to identify commercial sea cucumbers from selected locations in Indonesia by molecular approach. The targeted Cytochrome c oxidase subunit I (COI) was used in this research. A total of 25 beche-de-mers was purchased from local markets in Indonesia, i.e. Boalemo (17 local names), Pesawaran (5), Surabaya (2) and West Lombok (1). Results showed that 22 species of beche-de-mer were successfully identified, ie. Acaudina sp., Actinopyga echinites, A. lecanora, A. miliaris, Bohadschia argus, B. marmorata, B. ocellata, B. vitiensis, Holothuria sp. H. atra, H. coluber, H. edulis, H. excellens, H. fuscocinerea, H. fuscogilva, H. fuscopunctata, H. scabra, Pearsonothuria graeffei, Phyrella sp., Stichopus herrmanni, S. horrens and S. monotuberculatus. The identification of Indonesian beche-de-mers using molecular method could support the identification of the local sea cucumbers to overcome mislabeled and fraud. Correct identification is important for the record of beche-de-mer trade in Indonesia and further conservation management.
The genetic diversity and population structure of shallots (Allium cepa var. aggregatum) in Indonesia based on R gene-derived markers LINA HERLINA; REFLINUR REFLINUR; SOBIR SOBIR; AWANG MAHARIJAYA; SURYO WIYONO
Biodiversitas Journal of Biological Diversity Vol. 20 No. 3 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200312

Abstract

Abstract. Herlina L, Reflinur, Sobir, Maharijaya A, Wiyono S. 2019. The genetic diversity and population structure of shallots (Allium cepa var. aggregatum) in Indonesia based on R gene-derived markers. Biodiversitas 20: 696-703. Molecular markers are very useful for determining plant genetic diversity, especially when they are associated with useful traits. In shallots, type of markers still very limited. Therefore, development of new molecular markers is challenging if the future demand for this crop is to be met. The present study reports the genetic diversity analysis of 36 accessions of shallots using molecular markers derived from partial R gene sequences. Six polymorphic R gene-derived markers (Acepa-1, Acepa-2, Acepa-3, Acepa-4, Acepa-5, and Acepa-6) were developed and applied to analyze the genetic diversity of shallots in Indonesia. These loci produced a total of 963 alleles with an average of 0.51 + 0.77 alleles per marker. The polymorphic information content value ranged from 0.2784 to 0.5236 with an average of 0.36015. The markers were able to differentiate the shallot genotypes into three major clusters, suggesting that shallots are characterized by poor levels of genetic diversity in Indonesia. After further validation, the markers will be very useful, serving as tools to support the breeding of disease-resistant shallot varieties.
Short Communication: Correlation, path analysis, and heritability of phenotypic characters of bread wheat F2 populations YUDIWANTI WAHYU; NURWANITA EKASARI PUTRI; TRI KOESOEMA NINGTYAS; SURJONO HADI SUTJAHJO; AMIN NURa
Biodiversitas Journal of Biological Diversity Vol. 19 No. 6 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190644

Abstract

Wahyu Y, Putri NE, Trikoesoemaningtyas, Sutjahjo SH, Nur A. 2018. Short Communication: Correlation, path analysis, and heritability of phenotypic characters of bread wheat F2 populations. Biodiversitas 19: 2344-2352. Wheat varieties are still limited for Indonesia. A high yield wheat plant is necessary to be assembled by selecting plants from a segregated population. The selection will need information on some characters that contribute to the high yield and the relationship among the observed characters. This study aimed to determine the relationship between characters and the effects of the observed characters to grain yield per plant. This research was conducted from July 2017 to March 2018 at Balithi Experimental Farm (1100 m asl.), Cipanas, Cianjur District, West Java, Indonesia. The genetic material used were three F2 population of bread wheat (G1/Se, HP/Se, and Ja/Se) and four parent genotypes. Observations were made on each individual of F2 populations. Parent genotypes were planted by using completely randomized block design with three replications. The results showed that the number of a grain of non-main spike and the number of grain per plant characters had a high correlation in each F2 population and these characters also had positive and significant direct effect to grain weight per plant. It could be a character selection for high yield breeding program. The broad sense heritability was medium to a high value for all characters in F2 G1/Se and HP/Se populations.
Lichen diversity in geothermal area of Kamojang, Bandung, West Java, Indonesia and its potential for medicines and dyes JOKO KUSMORO; IIN SUPARTINAH NOER; MUHAMAD FEISAL JATNIKA; RIRIN EKA PERMATASARI; RUHYAT PARTASASMITA
Biodiversitas Journal of Biological Diversity Vol. 19 No. 6 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190643

Abstract

Kusmoro J, Noer IS, Jatnika MF, Permatasari RE, Partasasmita R. 2018. Lichen diversity in geothermal area of Kamojang, Bandung, West Java, Indonesia and its potential for medicines and dyes. Biodiversitas 19: 2335-2343. The study of lichens diversity in Kamojang, West Java was conducted by survey in geothermal field area following the line transect 6 km along to the East, North West and south from the Power House of Geothermal Power Plant. The lichen samples were taken from bark, soil, and stone. Lichen identification was done by morphological, anatomy and chemical analysis. Dyes potency of Parmotrema and Usnea test using ammoniac fermentation was done in Plant Taxonomy Laboratory of Department Biology, Faculty of Mathematics and Natural Sciences, University of Padjadjaran. The survey has successfully collected 133 species of lichens, belong to 62 genera and 17 families. Parmeliaceae was found as dominant groups, consisting of 33 species and other co-dominant groups are Graphidaceae and Lobariaceae with 24 species and 8 species, respectively. Most lichens in Kamojang geothermal area belong to Ascomycetes, only one Basidiomycetes such as Dictyonema sericeum (Sw.) which found at Kawah Manuk (Manuk crater) area. The rare species of lichens such as Usnea longissima Ach, was found at Pine forest in Arboretum 6 km south of Powerhouse of Kamojang geothermal. Chemical analysis and literature study for Lichenic acid contains was done and generally, atranorin, usnic acid, barbatic and lecanoric acid was found in lichens samples. Amoniac fermentation result showed that Parmotrema tinctorum produced brownish red, red and purple, which occurred within 1 week to 5 weeks after fermentation. While Usnea produced variety of brown color, which occurred within 5 days up to 4 weeks after fermentation. Lichen species containing some medical properties are Bulbothrix, Cladonia and Usnea. While lichens having dyes properties are Hypogymnia, Lobaria, Peltigera, Usnea, and Parmotrema.
Mass vegetative propagation of rare and endangered tree species of Indonesia by shoot cuttings by KOFFCO method and effect of container type on nursery storage of rooted cuttings HENTI HENDALASTUTI RACHMAT; ATOK SUBIAKTO; ARIDA SUSILOWATI
Biodiversitas Journal of Biological Diversity Vol. 19 No. 6 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190645

Abstract

Rachmat HH, Subiakto A, Susilowati A. 2018. Mass vegetative propagation of rare and endangered tree species of Indonesia by shoot cuttings by KOFFCO method and effect of container type on nursery storage of rooted cuttings. Biodiversitas 19: 2353-2358. The Forestry Research and Development Agency (FORDA) and The Advance Research of Komatsu Ltd have developed a mass propagation technique using shoot cuttings, namely KOFFCO system (Komatsu-FORDA Fog Cooling System). KOFFCO system controls environmental factors (temperature, humidity, and light intensity) to produce high percentage of rooted cuttings. The seed production of dipterocarp species is unreliable and unpredictable. Thus, vegetative propagation is an alternative means for producing planting materials of dipterocarp species. An attempt was made to produce rooted cuttings of rare and endangered dipterocarp and non-dipterocarp species of the Indonesian native trees, for further use in both reforestation and plantation programs. In fact, this study attempts to answer two specific research questions: (i) What is the potential of KOFFCO system as a vegetative mass propagation technique for Indonesian dipterocarps and non-dipterocarps? (ii) What is the effect of container types on long-term post-nursery storage of dipterocarp planting stock? To answer the first question stated above, vegetative propagation through shoot cuttings of 24 species belonging to 7 genera of dipterocarps and 13 species belonging to 12 genera of non-dipterocarps was attempted (>30.000 cuttings, in total), using KOFFCO system. The results showed that KOFFCO is an efficient innovative technology for the successful mass propagation from cuttings. 100% root formation obtained for Nauclea orientalis, Anotephalus macrophyllus and Ficus sp. To find the answer for the second question, storage experiments and growth observations of the planting stock of selected dipterocarp species was performed, using three different types of containers. Rooted cuttings of six species of dipterocarps with same germination date were tested in each type of the container. The result showed that medium sized square and medium sized round containers produced an average height increment of 19.92 cm and 21.58 cm, respectively, in a year, while small sized square containers produced only 13.94 cm height increment. Both square and round shaped containers are appropriate for short term storage of planting stock, while small containers are more appropriate for storing planting stocks or seedlings for a longer period.

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