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Biodiversitas Journal of Biological Diversity
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Articles 6,315 Documents
Short Communication: Genetic similarity analysis of in vitro cultivated pineapple (Ananas comosus) from Sipahutar, North Sumatra, Indonesia using ISSR markers FAUZIYAH HARAHAP; NUSYIRWAN NUSYIRWAN; ANNISA AFIVA; EKO PRASETYA; CICIK SURIANI; RIFA FADHILLAH MUNIFAH HASIBUAN; ROEDHY POERWANTO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 11 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231111

Abstract

Abstract. Harahap F, Nusyirwan N, Afiva A, Prasetya E, Suriani C, Hasibuan RFM, Poerwanto R. 2022. Short Communication: Genetic similarity analysis of in vitro cultivated pineapple (Ananas comosus) from Sipahutar, North Sumatra, Indonesia using ISSR markers. Biodiversitas 23: 5623-5628. Sipahutar pineapple from North Sumatra, Indonesia, is considered difficult to breed, resulting in a lack of seed availability. However, tissue culture allows the emergence of somaclonal variations, especially in plantlets undergoing several subculture stages. This study aimed to identify somaclonal variations in Sipahutar pineapple seedlings generated from in vitro culture using Inter Simple Sequence Repeat (ISSR) markers. Fifteen accessions were used in this study consisting of Sipahutar pineapple grown in its original environment, a laboratory environment, acclimatized, and in vitro cultured. Total of 10 primers, of which seven primers detected polymorphisms among the population. This study resulted in a low genetic similarity among the Sipahutar pineapple population. The genetic diversity of Sipahutar pineapple plants propagated by in vitro culture had a similarity level of 75%-94%, showing somaclonal variations in vitro culture. The genetic similarity of pineapple accessions was divided into two main clusters, namely cluster A consisted of 11 accessions and cluster B consisted of 4 accessions. Cluster separation does not correlate to the source of plant materials studied.
Tolerance response of varied tomato genotypes grown at excess manganese (Mn) SITI ZAHARA; NONO CARSONO; RIJA SUDIRJA; MOCHAMAD ARIEF SOLEH SOLEH
Biodiversitas Journal of Biological Diversity Vol. 23 No. 6 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230648

Abstract

Abstract. Zahara S, Carsono N, Sudirja R, Soleh MA. 2022. Tolerance response of varied tomato genotypes grown at excess manganese (Mn). Biodiversitas 23: 3209-3218. High Mn concentration in acidic soil has been known as a limiting factor of sustainable tomato production. In addition, the number of tomato cultivars available to be grown under such conditions is still limited. Therefore, this study tested the Mn tolerance response among four tomato genotypes, Opal, Mutiara, Ratna, and Mirah, grown hydroponically. Nutrient solutions were added to Mn for concentrations of 0, 50, 100, and 200 ppm. The increase of Mn concentration ([Mn]) significantly decreased chlorophyll fluorescence and stomatal conductance. Shoot Tolerance Index (STI) and Root Tolerance Index (RTI) among genotypes were significant differences in response to Mn concentration. Genotype Mirah was the lowest Mn accumulation in the shoot and root compared to other genotypes. Most of the Mn absorbed by Mirah was accumulated in the roots. The tolerance ranks of the tomato genotype tested for Mn toxicity were Mirah > Opal > Ratna > Mutiara. This indicates that genetic composition plays an essential role in Mn tolerance. The tolerance response of Mirah was due to its ability to prevent Mn from being absorbed by the shoot, which led to the effect of shoot toxicity. This response might help improve tomato genotypes tolerance under high Mn soil.
Taxonomy and molecular phylogeny analysis of the genus Diogenes “Edwardsii group” (Decapoda: Anomora: Diogenidae) based on mitochondrial DNA sequences TYANI FITRIAN; HAWIS MADDUPPA; MEUTIA SAMIRA ISMET; DWI LISTYO RAHAYU
Biodiversitas Journal of Biological Diversity Vol. 23 No. 10 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231040

Abstract

Abstract. Fitrian T, Madduppa H, Ismet MS, Rahayu DL. 2022. Taxonomy and molecular phylogeny analysis of the genus Diogenes “Edwardsii group” (Decapoda: Anomora: Diogenidae) based on mitochondrial DNA sequences. Biodiversitas 23: 5302-5313. Many variations of morphological characters differ from one species to another, showing high interspecific variation within the genus Diogenes Dana, 1851. Subtle morphological differences among certain species make it difficult to identify diagnostic characters. Although the genus is very speciose, the study on the molecular is poorly reported. This study aimed to evaluate the taxonomy and phylogeny of the genus Diogenes using morphological and molecular features. The molecular phylogeny of 15 species of Diogenes included in the "Edwardsii group" was constructed based on two mitochondrial genes COI and 16S rRNA. The results of the phylogeny tree construction based on concatenated mitochondrial genes COI and 16S rRNA mtDNA formed 5 clades of monophyletic. Phylogenetic relationships of Diogenes species based on concatenated mitochondrial genes COI and 16S rRNA sequenced suggest that the morphological and molecular analyses of the species in this study corroborated one another. Among 15 species studied, three species were challenging to identify due to their resemblance to other species traits. The phylogenetic tree demonstrates that the three taxa denoted by 'sp.' were different from the known species of Diogenes so far. Diogenes sp.1 was closely related to Diogenes moosai Rahayu & Forest, 1995, Diogenes sp.2 resembles Diogenes laevicarpus Rahayu, 1996, while Diogenes sp.3 was closely related to Diogenes goniochirus Forest, 1956.
Short Communication: Analysis of the ultimate wood composition of a forest plantation species, Eucalyptus pellita, to estimate its bioelectricity potency MUHAMMAD TAUFIQ HAQIQI; DUDU HUDAYA; HELMI ALFATH SEPTIANA; RICO RAMADHAN; YULIANSYAH YULIANSYAH; WIWIN SUWINARTI; RUDIANTO AMIRTA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 5 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230516

Abstract

Abstract. Haqiqi MT, Hudaya D, Septiana HA, Ramadhan R, Yuliansyah, Suwinarti W, Amirta R. 2022. Short Communicarion: Analysis of the ultimate wood composition of a forest plantation species, Eucalyptus pellita, to estimate its bioelectricity potency. Biodiversitas 23: 2389-2394. Eucalyptus pellita F. Muell is one of the short rotation wood crop species widely planted in tropical countries, including Indonesia. Woody biomass obtained from this species is commonly utilized to produce fiber in the pulp and paper industry. Due to the growing interest in expanding E. pellita plantations, the potential application of E. pellita woody biomass to provide sustainable energy feedstock has been studied. Therefore, this study aimed to investigate the ultimate composition of E. pellita wood (carbon (C), hydrogen (H), and oxygen (O)) to estimate its higher heating value (HHV) and bioelectricity potency. The wood samples were harvested at different plant ages, from the first to the fifth year. The percentage of biomass composition, including cellulose, hemicellulose, lignin, and extractives, was also calculated. The results demonstrated that lignin in the E. pellita wood increased to align with the increased plant age. Thus, this pattern was followed by significantly increased C content in the wood since lignin contained a primary source of C. Hence, this condition might enhance the HHV and electricity potency. The ratio of H/C and O/C was found to be one of the most promising factors in improving HHV compared to the extractive/lignin ratio. In the fifth year, the electricity potency of E. pellita showed the highest value (1.71 MWh ton-1). Therefore, this study suggests that E. pellita possesses the potential to be one of the promising crops for green electricity production.
Freshwater gastropod community in South Konawe District, Southeast Sulawesi, Indonesia DEDY OETAMA; MUHAMMAD FAJAR PURNAMA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 7 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230707

Abstract

Abstract. Oetama D, Purnama MF. 2022. Freshwater gastropod community in South Konawe District, Southeast Sulawesi, Indonesia. Biodiversitas 23: 3364-3372. The freshwater gastropod population in South Konawe District, Southeast Sulawesi, Indonesia, consists of aquatic and terrestrial snail communities. This resource (gastropod) is an ecological and economic potential that needs to be maintained and utilized optimally and sustainably. This research was conducted in April-July 2021 and aimed to determine the ecological index of the inland gastropod community in the South Konawe District. The sampling location was determined qualitatively using the purposive sampling method and a simple random sampling technique (Quantitative based). Gastropod samples were collected manually (hand picking) with a paralon pipe (3.5 inches), Sieve (1 mm), and Gloves. The total gastropods obtained at 33 sampling locations in this district were 2635 individuals. The inland gastropods comprised 19 genera and 33 species spread over 9 families, namely Achantinidae, Ampullariidae, Ariophantidae, Lymnaeidae, Neritidae, Pachychilidae, Planorbidae, Thiaridae, and Viviparidae. The average abundance of gastropods reached 79.85 ind./m2 and the abundance of gastropod species ranged from 0.58-13.48 ind./m2. Overall, the analysis of gastropod ecology index showed a stable condition, where the diversity of gastropod species was categorized as high (H'= 3.03), moderate evenness (E= 0.87), and high species richness (R= 4.06). There were no dominant species (C= 0.08) and the gastropod distribution showed a clustered pattern (Ip= 0.02). These ecological index values indicated that the gastropod community in the inland waters of South Konawe District was in a stable condition. Although the gastropod community in the inland waters of the South Konawe District simultaneously has the same area as the invasive gastropod from the family Thiaridae, namely Melanoides tuberculata (O.F.Müller, 1774) and Tarebia granifera (Lamarck, 1816).
Diversity, distribution, and nest characteristics of stingless bees (Hymenoptera: Meliponini) in Baluran National Park, East Java, Indonesia Regina Diah Rachmawati; Ali Agus; Nafiatul Umami; Agussalim Agussalim; Hari Purwanto
Biodiversitas Journal of Biological Diversity Vol. 23 No. 8 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230805

Abstract

Abstract. Rachmawati RD, Agus A, Umami N, Asgussalim, Purwanton H. 2022. Diversity, distribution, and nest characteristics of stingless bees (Hymenoptera: Meliponini) in Baluran National Park, East Java, Indonesia. Biodiversitas 23: 3890-3901. Baluran National Park is a conservation area located in Situbondo, East Java. It has become a natural habitat for many animals, including stingless bees. Stingless bees, known as klanceng in Java, are eusocial insects that produce honey and play an important role as pollinators. This study aimed to explore the diversity of stingless bees in BNP, East Java. Two species of stingless bees were found in nine sites with various types of habitats: teak forest, secondary forest, evergreen, savanna, mangrove, and settlements. Based on their morphological and morphometric characteristics, the bees were identified as Lepidotrigona terminata and Tetragonula laeviceps. The results of species identification were confirmed by molecular analysis using the 16S rRNA gene from mitochondrial deoxyribonucleic acid. Furthermore, we described the distinction of nest entrance appearances of stingless bees from their natural nest and beehive in meliponiculture. The stingless bee Tetragonula laeviceps is considered an adaptive stingless bee because it is distributed in all types of habitats in this study. These data would add information to the biology and distribution of stingless bee species in Indonesia, especially in Java Island, and provide bases for their utilization in the future.
The application of the River Continuum Concept (RCC) on the headwaters of the Sampolawa River in Southeast Sulawesi, Indonesia LA ODE MUHAMMAD ERIF; TJUT SUGANDAWATY DJOHAN; SUDARMADJI SUDARMADJI; ASRAMID YASIN; LA BACO SUDIA; KAHIRUN KAHIRUN
Biodiversitas Journal of Biological Diversity Vol. 24 No. 1 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240135

Abstract

Abstract. Erif, LOM, Djohan TS, Sudarmadji, Yasin A, Sudia LB, Kahirun. 2023. The application of the River Continuum Concept (RCC) on the headwaters of the Sampolawa River in Southeast Sulawesi, Indonesia. Biodiversitas 24: 290-299. Benthic aquatic insect communities in temperate regions have been used as bioindicators of a healthy forest in the stream headwaters.This research analyzes the abundance of benthic insects, the quality of water and nutrient sediment, and the response of the insect community in the Sampolawa River. The samples were collected using stratified random sampling methods with a Surber sampler 30 cm x 30 cm, 5 segmented streams, and 200 m with 4 replication. The results show that 19 species of 5 community feeding groups were found, including collector filters, gatherers, predators, scrapers, and shredders. However, the shredders community is very small, at about 2-4 %. The benthic insect aquatic dominance by collector filter with 29 % reflected that input from terrestrial vegetation is Fine Particulate Organic Matter (FPOM). Therefore, it can be deduced that Sampolawa headwaters do not fit with the prediction of the "River Continuum Concept (RCC)"for a typical headwater since the allochthonous energy supply from riparian vegetation is in the form of fine detritus.
New distribution record of Varuna litterata from Caraga Region, Philippines: Analysis on morphometry, length/width-weight relationship, and condition factor Jess H. Jumawan; Jeco Jed J Ruales; Maria Cristina A Avila
Biodiversitas Journal of Biological Diversity Vol. 23 No. 6 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230620

Abstract

Abstract. Jumawan JH, Ruales JJJ, Avila MCA. 2022. New distribution record of Varuna litterata from Caraga Region, Philippines: Analysis on morphometry, length/width-weight relationship, and condition factor. Biodiversitas 23: 2935-2942. The study reported the occurrence of Varuna litterata in its habitat as a new record in Caraga Region, Philippines. The crab samples were collected using a hand-picked method and were of marketable sizes. There were 14 morphometric traits measured with the corresponding weight and sex of each specimen. Majority of the crab samples were medium-sized, with weight ranging from 11-20 grams, a carapace width range of 28-34 cm, and a carapace length range of 27-32 cm. Exploratory analysis of length /width-weight data fits the power regression model depicted into graphs. A strong relationship and highly significant was observed on pooled samples of WT-CW variables (R2: 0.9229: P<0.001) and WT-CL variables (R2: 0.9197); P<0.001). The derived log-transformed for WT-CW relationship was logWT: -2.7994+2.6456 logCW. Results suggest that carapace length and width tend to be smaller relative to the weight, which is an indication of a negative allometric growth pattern. The computed Fulton's condition factor was higher in males than females, as supported by Kruskal Wallis test (P<0.001). The data provides baseline information for comparing similar studies and in understanding the population dynamics of this crab species.
Characteristic of the material deposits, microclimate profile, epiphyte abundance, and bacteria populations in the above-ground ecosystem of oil palm IPUT PRADIKO; RANA FARRASATI; SUROSO RAHUTOMO; FADILLA SAPALINA; RIZKI DESIKA PUTRI PANE; FANDI HIDAYAT; EKO NOVIANDI GINTING
Biodiversitas Journal of Biological Diversity Vol. 23 No. 7 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230756

Abstract

Abstract. Pradiko I, Farrasati R, Rahutomo S, Sapalina F, Pane RDP, Hidayat F, Ginting EN. 2022. The physical, chemical, and biological characteristics of microhabitats inside oil palm trunk axils in North Sumatra, Indonesia. Biodiversitas 23: 3793-3807. Above-ground ecosystems (AGE) components in oil palm plantations consist of epiphytic plants and fauna in microhabitat within the axil of pruned fronds. This research aimed to identify the characteristics of microhabitat, including microclimate profile, physical and chemical properties of the material deposit, epiphyte abundance, and microbe population. The study sites were located in the eastern and western regions of North Sumatra. Furthermore, vertical observations were taken on oil palm trunks at the height of 20, 100, 150, 200, and 300 cm. The study indicated that the microclimate, nutrients content, physical properties of the material deposit, and bacterial populations in the trunk axils of oil palm in the eastern differed from the western region. There was no obvious pattern in the microclimate profile for individual oil palms. However, there was a change in N, C-organic, and C/N ratios with the increasing height of microhabitat sites. In terms of abundance, Nephrolepis bisserata and Vittaria ensiformis epiphytes were more dominant in the upper and bottom trunk, respectively. The microhabitat at the bottom trunk had a denser bacterial population, negatively correlated with N, C-organic, C/N ratio, Mg, and pH.
Biological control of Sclerotinia minor attack on pyrethrum plants by Trichoderma harzianum in glasshouse experiment Yan Ramona; Ida Bagus Gede Darmayasa; Martin A Line
Biodiversitas Journal of Biological Diversity Vol. 23 No. 6 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230655

Abstract

Abstract. Ramona Y, Darmayasa IBG, Line MA. 2022. Biological control of Sclerotinia minor attack on pyrethrum plants by Trichoderma harzianum in glasshouse experiment. Biodiversitas 23: 3264-3269. The aim of this research was to elucidate the efficacy of Trichoderma harzianum (isolate Td22) grown in a ratio of 2:8 millet seeds and wood fiber waste (WFW) compost mixture to suppress Sclerotinia minor infection in pot trails on the pyrethrum plants (in 0.5 L pots). The pots were filled with soil and mixed with Td22-grown WFW compost to obtain a concentration of 5% v/v. The S. minor (fungal pathogen) previously grown in millet seeds amounted at 2.0 g per pot, was then evenly inoculated at 2 cm below the surface of potting mix. Soil without compost amendment, amended with pathogen only, or without pathogen inoculation served as controls. All pots were acclimatized for 4 days in a shade house prior to transplanting (4 seedlings per pot) of pyrethrum seedlings (aged of 3 weeks). Eight replications per treatment were run for 8 weeks. The results showed that 5% v/v compost-grown Td22 provided 78% protection to pyrethrum plants at week 8. Each surviving plant in Td22-treated pots also showed significantly higher average dry weight (p<0.05) than those planted in S. minor control treatment, indicating that Td22 has a potential to be developed as a novel fungal antagonist or a plant growth-promoting fungus.

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