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Biodiversitas Journal of Biological Diversity
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Articles 6,269 Documents
Agronomic and morphological characteristics of two rice genotypes plant in open land and under two years of sengon (Paraserianthes falcataria) DULBARI DULBARI; ZAINAL MUTAQIN; HERY SUTRISNO; NI SILUH PUTU NURYANTI; YURIANSYAH YURIANSYAH; DENNY SUDRAJAT; DESTIEKA AHYUNI; HIDAYAT SAPUTRA; LINA BUDIARTI; PRIYADI PRIYADI; FAJAR ROCHMAN; RIZKY RAHMADI; MUHAMMAD ALAM FIRMANSYAH; SAIJO SAIJO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240935

Abstract

Abstract. Dulbari, Mutaqin Z, Sutrisno H, Nuryanti NSP, Yuriansyah, Sudrajat D, Ahyuni D, Saputra H, Budiarti L, Priyadi, Rochman F, Rahmadi R, Firmansyah MA, Saijo. 2023. Agronomic and morphological characteristics of two rice genotypes plant in open land and under two years of sengon (Paraserianthes falcataria). Biodiversitas 24: 4927-4933. The increase in population is the biggest challenge for the agricultural sector in providing food needs. The main problem in increasing food production in Indonesia is the limited agricultural land. There is a need to explore alternative land options to address this issue and enhance production capacity, specifically for rice at the national level. One of the potential solutions is to use land currently occupied by plantation crops and forests that can be managed through agroforestry. Sengon (Paraserianthes falcataria (L) I.C.Nielsen) is a forestry plant that offers a comparative advantage for investigation in agroforestry systems due to its relatively open canopy cover and classification as a legume. Therefore, this research aimed to determine the response of the morphological and agronomic characters of two rice genotypes planted in open land under 2-year-old sengon stands. The experiment was conducted from October 2017 to March 2018 in the Sengon community forest of Cikarawang, Bogor, with coordinates 06° 33.061' S and 106° 43.987' E. The results showed that two rice genotypes grown under one-year-old sengon stands experienced decreased productive tillers, plant height, stem strength, and the number and weight of grains per panicle. The IR 64 genotype decreased by 40.65% in grain weight per panicle, while the Situ Patenggang genotype experienced a 56.21% decrease.
The potential of bitter melon extract on cellular non-specific immunity of koi fish (Cyprinus carpio) after Aeromonas salmonicida infection SRI ANDAYANI; MOH. FADJAR; HENY SUPRASTYANI; AULIA RAHMAWATI; NURHALISA NURHALISA; VINA NUR NADIRO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 10 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241001

Abstract

Abstract. Andayani S, Fadjar M, Suprastyani H, Rahmawati A, Nurhalisa, Nadiro VN. 2023. The potential of bitter melon extract on cellular non-specific immunity of koi fish (Cyprinus carpio) after Aeromonas salmonicida infection. Biodiversitas 24: 5215-5222. Indonesia has rich and potential fisheries resources both in freshwater (inland), coastal, and ocean. Potential fishery resources in freshwater include the diversity of fish species (germplasm) and fishery land. Utilization of freshwater fisheries potential can be realized by conducting fish farming, especially for economically valuable fish commodities, such as koi fish (Cyprinus carpio). Improving koi fish farming can be done by using bitter melon extract as an immunity enhancer. This study was conducted with the aim to determine the effect of bitter melon extract on cellular non-specific immune response. This study used a completely randomized design (CRD) method, with 4 treatments and 3 replicates, consisting of treatment K = not given bitter melon extract and not infected, A = dose of bitter melon extract 100 ppm, B = bitter melon extract 150 ppm, C = bitter melon extract 200 ppm. Before being given the treatment, the test fish were acclimatized with the aim of ensuring that the fish were healthy, not stressed and did not experience death. In addition, the dose of extract treatment and soaking time have been through the LD50 test as well as the density of bacterial infection and the length of time of infection have been through the LC50 test so that it has been confirmed that it does not cause stress and death in fish, with results SR 100%. A total of 10 fish were immersed into each treatment container (15 liters of water mixed with the extract) for 60 hours. Infection of Aeromonas salmonicida bacteria by immersion was carried out 5 days after immersion in bitter melon extract with a bacterial density of 107 cells.mL-1 for 1 hour. The results showed that lymphocytes increased after being given bitter melon extract and infected with A. salmonicida bacteria with a value of 63-83%. Monocytes increased from 3% to 8%, and Neutrophils increased from 4% to 9%. Phagocytosis activity increased from 18.4% to 28.9%, indicating that bitter melon extract can enhance non-specific immunity.
Length-weight relationship, condition factor, and reproductive aspects of Lutke's halfbeak Hemiramphus lutkei (Valenciennes, 1847) from Seram Sea, Maluku, Indonesia MATHEOS TALAKUA; SUADI SUADI; DJUMANTO DJUMANTO; EKO SETYOBUDI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 12 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241219

Abstract

Abstract. Talakua M, Suadi, Djumanto, Setyobudi E. 2023. Length-weight relationship, condition factor, and reproductive aspects of Lutke's halfbeak Hemiramphus lutkei (Valenciennes, 1847) from Seram Sea, Maluku, Indonesia. Biodiversitas 24: 6574-6583. Lutke's halfbeak is one of the Seram Sea's most commercially important fish species, providing food resources and community livelihood for an extended duration. Data and information on fish biology are important for responsible fisheries management. This research aimed to determine Luke's halfbeak's length-weight relationship, condition factor, and reproductive aspects at Seram Sea, Maluku. Fish samples were collected monthly for seven months, from August 2021 to February 2022. Each sample was measured for its length and weight and then dissected for sex and gonad maturity stage determination. The data was analyzed to determine the length-weight relationship, the condition factor, the distribution of gonadal maturity stage, and fecundity. Hemiramphus lutkei (Valenciennes, 1847), originating from the Seram Sea, indicates a negative allometric growth pattern. The average relative condition factor (Kn) of H. lutkei in the Maluku Seram Sea was around 1 and did not show a significant difference between males and females. The condition factor varied during the observation period, ranging from 0.92 to 1.09. The fish are mostly (80%) in good or very good condition. H. lutkei has a high potential fecundity; spawning was predicted throughout the year, with individual fecundity ranging from 263 to 1893 eggs. Luke's halfbeak populations in the Seram Sea could be exploited sustainably; however, this should be conducted with proper management.
Evaluating the quality of aquatic environment on the Planktonic-Index of Biotic Integrity (P-IBI) based whiteleg shrimp Litopenaeus vannamei (Boone, 1931) ponds MUHAMMAD AKBARURRASYID; VINI TARU FEBRIANI PRAJAYATI; ACHMAD SOFIAN; MUSTIKA KATRESNA
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240926

Abstract

Abstract. Akbarurrasyid M, Prajayati VTF, Sofian A, Katresna M. 2023. Evaluating the quality of aquatic environment on the Planktonic-Index of Biotic Integrity (P-IBI) based whiteleg shrimp Litopenaeus vannamei (Boone, 1931) ponds. Biodiversitas 24: 4845-4853. Plankton is one of the biotic indicators used to evaluate the quality of the aquatic environment in the ponds. This study aims to find out the quality of the aquatic environment of the whiteleg shrimp Litopenaeus vannamei (Boone, 1931) ponds using Planktonic-Index Biotic of Integrity (P-IBI). Samples were collected in intensive L. vannamei farming in Pandeglang, Banten, and analyzed by the P-IBI method using 4 categories and 35 attributes. Matrix attributes include community composition, number of species, species density and species diversity. The P-IBI value is compared with the assessment category to obtain the status of aquatic environmental quality. The environmental quality assessment categories are: very good (83-100%), good (73-82%), moderate (57-72%) and bad (0-56%). The results of the study showed the assessment of Community Composition (CC) of 66 (66%) and the scoring of Amount of Species (AS) of 65 (59.09%) on the attributes of the number of Bacilloryophycea, percentage of Cyanophycea, Dinophyceae, and Protozoa. The scoring of Species Density (SD) obtained 65 (59.09%) on attributes of the abundance of Chlorophyceae and Bacilloryophycea and the low abundance of Dinophyceae and Protozoa. Meanwhile, the scoring of Species Diversity (SDI) obtained a maximal score of 30 (100%), which showed that the species diversity of plankton based on the Shannon-Wiener Index (H'), Margalef Index (E), and Pielou Index (D) was still in good condition, the species diversity was stable, and there were no dominating species. The P-IBI score obtained was in the moderate category for L. vannamei farming activities.
Forests over limestone in Guiuan, Eastern Samar, the Philippines: Species composition and influencing environmental factors INOCENCIO E. BUOT JR.; ELAINE LOREEN C. VILLANUEVA; MARNE G. ORIGENES; REN DIVIEN R. OBEÑA
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240957

Abstract

Abstract. Buot Jr IE, Villanueva ELC, Origenes MG, Obeña RDR. 2023.  Forests over limestone in Guiuan, Eastern Samar, the Philippines: Species composition and influencing environmental factors. Biodiversitas 24: 5123-5136. Forests over limestone are critical ecosystems providing numerous ecosystem services. However, the ecology of these ecosystems is less studied like the Guiuan Marine Resource Protected Landscape and Seascape (GMRPLS) in Eastern Samar, Philippines which incidentally is affected by strong typhoons. Studies on forests over limestone are even more essential to understand the vegetation ecology providing insights in crafting appropriate management strategy to sustain ecosystem services. Hence, the study aims to identify the woody vegetation diversity and composition of forests over limestone in GMRPLS, and to analyze the environmental factors influencing the physiognomy and ecology of the forests. Standard vegetation techniques were used and a total of nine plots with size of  20 × 20m each were established to assess the tree species (?1m height). Plant abundance data were recorded and computed. Soil samples were collected while climate data were obtained from the Guiuan, Eastern Samar Weather Station. Cluster analysis using relative density of trees per plot was done in order to identify major plant communities. Subsequently, a canonical correspondence analysis was carried out to pinpoint which environmental variables influenced the plant data set. Results show a total of 37 species belonging to 17 families, with 18 species are listed under by IUCN and seven species are listed by DAO 2017-11. Cluster analysis indicated 3 vegetation groupings or clusters named after the dominant species per plot, namely Cluster I (Hancea-Calophyllum-Macaranga-Ficus-Gnetum), Cluster II (Monoon-Wallaceodendron-Artocarpus-Hancea-Bago-eho), and Cluster III (Bridelia-Monoon-Neonauclea-Gnetum-Artocarpus-Sterculia-Hancea). GUI 02 plot (Cluster 1) was observed as the most diverse with the highest Simpson’s index and Fisher’s alpha, as well as the highest in total number of tree species. Canonical correspondence analysis indicated elevation, temperature and anthropogenic disturbances as the major factors influencing the vegetation diversity and composition. This can be used to formulate forest management strategies for the sustainable conservation of forests over limestone in GMRPLS.
Community structure of periphyton on bamboo substrate in whiteleg shrimp (Litopenaeus vannamei Boone, 1931) pond with semi-biofloc system DIANA ARFIATI; UMI ZAKIYAH; SEPTI ANITASARI; ZAKIYYAH NUR INAYAH; RIZKY KUSMA PRATIWI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240952

Abstract

Abstract. Arfiati D, Zakiyah U, Anitasari S, Inayah ZN, Pratiwi RK. 2023. Community structure of periphyton on bamboo substrate in whiteleg shrimp (Litopenaeus vannamei Boone, 1931) pond with semi-biofloc system. Biodiversitas 24: 5080-5087. Periphyton is one aquatic organism that attaches to the substrate and can be used as a bioindicator. This study analyzes the community structure and dynamics of periphyton on bamboo substrates in whiteleg shrimp ponds with a semi-biofloc system. The study was conducted from May to July 2022. The bamboo substrate was placed in the ponds, and the samples were taken every seven days to 8 weeks. Identification and calculation of periphyton using Lackey Drop Micro transect Counting Method. The periphyton was found on a bamboo substrate in whiteleg shrimp ponds with a semi-biofloc system consisting of phyto-periphyton and zoo-periphyton. The phyto-periphyton group contains 4 phylum, 21 species, including the Bacillariophyta, Chlorophyta, Cyanophyta, and Ocrophyta. The zoo-periphyton group has 4 phylum, 10 species consisting of Arthropoda, Ciliophora, Nematoda, and Rotifera. The abundance of periphyton found changes every week. The relative abundance of phyto-periphyton includes phylum Bacillariophyta (17-69%), Chlorophyta (1-22%), Cyanophyta (30-65%), and Ocrophyta (0-25%), while zoo-periphyton includes phylum Arthropoda (0-3%), Ciliophora (69-99%), Nematodes (0-2%) and Rotifers (0-28%). The total abundance of phyto-periphyton on bamboo substrate ranged from 223,806-989,398 cells/L, while zoo-periphyton ranged from 396-1,924 individual/L. The diversity, uniformity, and dominance index of phyto-periphyton and zoo-periphyton are moderate. Water quality parameters during the study were relatively good for aquatic organism growth, except for very high total organic matter levels in several weeks.
Utilization of pretreated oil palm empty fruit bunches and their hydrolysate for ethanol production by Indonesian ethanologenic yeast SITA HERIS ANITA; MAULIDA OKTAVIANI; EUIS HERMIATI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 10 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241004

Abstract

Abstract. Anita SH, Oktaviani M, Hermiati E. 2023. Utilization of pretreated oil palm empty fruit bunches and their hydrolysate for ethanol production by Indonesian ethanologenic yeast. Biodiversitas 24: 5243-5252. Oil Palm Empty Fruit Bunches (OPEFB) represent a polysaccharide-rich raw material with promising potential for ethanol production. This study aimed to investigate the ethanologenic yeasts, specifically Saccharomyces cerevisiae InaCC Y93 and Kluyveromyces marxianus InaCC Y119, affect bioethanol production in three different systems: Separate Hydrolysis and Fermentation (SHF), Simultaneous Saccharification and Fermentation (SSF), and Prehydrolysis-Simultaneous Saccharification and Fermentation (PSSF). This work is distinguished by the use of indigenous Indonesian yeast strains, including a thermotolerant strain. In the pretreatment process, 1.13% oxalic acid was added to OPEFB and subjected to microwave treatment at 190°C for 3.01 min. Subsequently, cellulase enzymes (40 FPU/g) and a 10% (w/v) yeast inoculum were introduced into 5.27 g dry weight of pretreated OPEFB pulp. The OPEFB acid hydrolysate was also subjected to fermentation. Ethanol content was monitored at 24 h intervals for 72 h. The PSSF system employs K. marxianus InaCC Y119 at 48 h exhibited the highest ethanol concentration, yielding 0.290 g/g, equivalent to approximately 51.20% of the theoretical yield. Additionally, K. marxianus InaCC Y119 demonstrated its capability to ferment the OPEFB acid hydrolysate into ethanol. These findings underscore the considerable potential of K. marxianus for applications in fermenting both hexose and pentose sugars to produce ethanol within higher-temperature systems.
Genetic diversity and molecular analysis using RAPD markers of banana cultivars in the five regions of East Java, Indonesia SLAMETO SLAMETO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240947

Abstract

Abstract. Slameto. 2023. Genetic diversity and molecular analysis using RAPD markers of banana cultivars in the five regions of East Java, Indonesia. Biodiversitas 24: 5035-5043. The study explored banana plants in East Java, Indonesia, specifically in the districts of Jember, Banyuwangi, Bondowoso, Situbondo, and Lumajang, using Random Amplified Polymorphic Deoxyribonucleic Acid (RAPD) markers. Thirty accessions of Musa ssp. were collected from these five districts, revealing a high level of genetic diversity. Primers OPA-04 and OPC-05 were suitable for assessing genetic variation in banana plants. It was found that the OPD-07 primer produced the fewest DNA bands (8 bands and 7 polymorphic bands), while the OPA-04 primer produced the maximum number of DNA bands (14 bands, with 9 polymorphic bands). The OPB-08 primer had the lowest polymorphism percentage (56%), and the OPC-05 primer had the greatest percentage (92%). With genetic similarity scores ranging from 0.04 (Bigih Tanjung Glugur and Lilin Banyuwangi) to 0.94 (Agung Banyuwangi and Let). The phylogenetic tree of 30 banana plants formed two primary clusters: cluster I and II, included 26 and 4 banana varieties respectively. In addition, molecular variance (AMOVA) resulted a significant proportion (93%) of genetic diversity within the population. Further studies using more precise genetic markers are still needed to determine the exact identity of the banana plant genome.
Tillage systems affect soil nitrogen availability and interact growth parameters of different faba bean cultivars AHMED F. M. AL-ENEZY; BASIM A. ZAIDAN; ALI F. ALMEHEMDI; ALAA A. HUSSEIN; MUSTAFA ABED AL-JABBAR SALIH
Biodiversitas Journal of Biological Diversity Vol. 24 No. 10 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241037

Abstract

Abstract. Al-Enezy AFM, Zaidan BA, Almehemdi AF, Hussein AA, Salih MAA. 2023. Tillage systems affect soil nitrogen availability and interact growth parameters of different faba bean cultivars. Biodiversitas 24: 5558-5564. A field trial was conducted during the winter season of 2021-2022 in one of the agricultural lands of Hamid Shaban village, which belongs to Abu Ghraib District of Baghdad Province (33°17'51.3"N, 44°04'55.0"E). The aim of the experiment was to investigate nitrogen availability in two different tillage systems and the growth characteristics of six broad bean varieties on clay-loamy soils. The field trial was triplicated using the Randomized Complete Block (RCBD) method. The first factor was the six broad bean varieties (Turkish, Dutch, Italian, Spanish, American and local), the second factor was the cultivation system Conservation Tiltage (no-till) and the traditional cultivation with a mouldboard plow. The results showed that the availability of nitrogen in the soil in the traditional tillage system corresponds to the superior to conservative tillage systems. Where this gives an average available nitrogen concentration of 23.3 mg kg-1, an increase of 21 in relation to conservation tillage, which resulted in an average concentration of available nitrogen of 19.1 mg kg-1. In addition, all the growth characteristics of the plants favored the traditional agricultural system. In the variety comparison, the Italian variety was characterized by the highest nitrogen content available in the soil of 26.10 mg kg-1 as well as the highest values ??of plant growth properties among the six varieties.
Diversity and abundance of ants (Hymenoptera: Formicidae) as indicators of sugarcane agroecosystem stability in Blitar, East Java, Indonesia SUJAK SUJAK; BAMBANG TRI RAHARDJO; FAIZ NASHIRUDDIN MUHAMMAD; AKHMAD RIZALI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 10 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241015

Abstract

Abstract. Sujak, Rahardjo BT, Muhammad FN, Rizali A. 2023. Diversity and abundance of ants (Hymenoptera: Formicidae) as indicators of sugarcane agroecosystem stability in Blitar, East Java, Indonesia. Biodiversitas 24: 5336-5342. The abundance of ant communities can be used as an indicator of stability in agroecosystems. This study was designed to evaluate the diversity and abundance of ant in three sugarcane cultivation ecosystems. The research was conducted in Ngembul Village, Binangun Subdistrict, Blitar, East Java, Indonesia from August 2021 to February 2022 The three observed sugarcane ecosystems were Ratoon Cane (RC) without tillage, Replanting Ratoon Cane (PRC) with tillage, and Newly Cultivated Plant Cane (NPC) with tillage. Ant collections were made using the pitfall trap method. In each field, pitfalls were systematically placed at 5 points. Pitfall traps were set for 24 hours, and samples were collected every 2 weeks. Identification was done at the genus level and further separated based on morphology (morphospecies). The research obtained 2,920 ant individuals belonging to 3 subfamilies, 7 genera, and 9 morphospecies. The highest diversity and abundance were found in the RC ecosystem compared to the PRC ecosystem and the NPC agroecosystem. The most dominant ant species was Crematogaster sp.1. The ant population in the RC ecosystem has more population growth compared to the PRC and NPC ecosystems. The results of the ANOSIM analysis indicated differences in ant community composition among the treatments. In conclusion, the ratoon sugarcane ecosystem sustains diversity and abundance of ants more compared to the other two ecosystems.

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